ARTIFICIAL OOCYTE ACTIVATION IMPROVES THE LABORATORY AND CLINICAL OUTCOME IN VITRIFIED DONOR OOCYTE GROUP

Introduction: Oocyte donation is proved effective. Vitrification of donor eggs allows creation of donor egg banking. Simultaneously, for good clinical outcome it is recommended to thaw 10-15 oocytes at once. In the current study, we demonstrate the benefit of using artificial oocyte activation in order to reduce the number of thawed donor eggs for IVF program without any affect on laboratory and clinical outcome. Aim of study: To improve the good quality blastocyst formation rate using artificial activation with vitrified donor eggs. Is it possible to increase the clinical pregnancy rate (CPR) and live birth rate (LBR) thawing only 6-8 donor eggs? Materials and Methods: The retrospective cohort studyincluded 40 fresh (Group A) and 12 vitrified (Group B) donor egg programs. ICSI was conducted to all oocytes. In Group B, we also used artificial oocyte activation with calcium ionophore. Student T test was used to infer statistical significance. P value < 0.05 was considered significant. Results: The fertilization and good quality blastocyst formation rate is not different between the groups. The majority of usable blastocysts, 72% in Group A and 93% in Group B were formed on Day 5. The CPR is not statistically different between groups A and B and is 52.5% and 50% respectively. The IR is not statistically significant and is 39% in Group A and 42% in Group B. The LBR is higher in Group A (50%) comparing to Group B (25%), but the difference is not statistically significant. Conclusions: Considering our data, we suggest that artificial oocyte activation is feasible for use with vitrified donor eggs. It might decrease the expenses of patients on thawing less number of donor oocytes without negative impact on the laboratory and clinical outcome.

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P–082 Effect of semen hyper viscosity (SHV) on blastocyst formation rate and implantation rate

Human Reproduction ◽

10.1093/humrep/deab130.081 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

A Suthar ◽

N Sharma ◽

V Mishra ◽

R Aggarwal ◽

H Sheth ◽

...

Keyword(s):

Seminal Fluid ◽

Semen Analysis ◽

Formation Rate ◽

Implantation Rate ◽

Fertilization Rate ◽

Accessory Gland ◽

Blastocyst Formation ◽

Registration Number ◽

Group A ◽

Group B

Abstract Study question Does semen hyper viscosity effects blastocyst formation rate Summary answer Hyper viscosity of semen sample later results in poor blastocyst formation rate and lower implantation rate. What is known already Normal range of semen hyper viscosity ranges between 12–29%.Highly viscous semen samples impairs the physical and chemical characteristics of seminal fluid and due to which seminal oxidative damage increases which further increases the ROS and reduces the sperm motility there are some factors that can affect the seminal viscosity out of which one is Male accessory gland infection, Hypo function of prostate seminal vesicles and varicoceles. SHV create hindrance in semen preparation. Study design, size, duration Retrospective study was conducted from June 2019 to Oct 2020 at IVF unit IKDRC hospital. Participants/materials, setting, methods 142 patients were enrolled from June 2019 to Oct 2020 in IVF unit IKDRC hospital and divided into two groups. Group A (n = 83) patients with hyper semen viscosity and Group B (n = 69) patients with normal semen viscosity, inclusion and exclusion criteria’s were same for both the groups, only patient with normozoospermia were taken. Semen analysis was done by using WHO manual 2010. Main results and the role of chance In group A with hyper semen viscosity fertilization rate was (49.2% vs. 70% p = <0.001) vs in group B with normal semen viscosity which is significantly higher in group B, Blastocyst formation rate ( 18.4% vs 35% p = <0.01) and implantation rate (9.4% vs 20% p = <0.005) both are significantly higher in group B . Which implies fertilization rate , blastocyst formation rate and implantation rate is significantly lower in patients with semen hyper viscosity. Limitations, reasons for caution Larger randomized control studies are needed to strengthen these results. Wider implications of the findings: Our study demonstrates that patients having higher semen viscosity have poor blastocyst formation rate and implantation rate due to oxidative stress. Trial registration number Not applicable

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Increasing dominant follicular proportion negatively associated with good clinical outcomes in GnRH-a prolonged protocol: a large-sample retrospective analysis

10.21203/rs.3.rs-870140/v1 ◽

2021 ◽

Author(s):

Houming Su ◽

Youhua Lai ◽

Jie Li ◽

Tingting Liao ◽

Licheng Ji ◽

...

Keyword(s):

Regression Analysis ◽

Linear Regression ◽

Retrospective Analysis ◽

Formation Rate ◽

Clinical Pregnancy ◽

Blastocyst Formation ◽

Negatively Associated ◽

Large Sample ◽

Group A ◽

Group B

Abstract Background: Nowadays, there is no universal criteria for trigger time during controlled ovarian hyperstimulation (COH). Particularly, in the so-called GnRH-a prolonged protocol, widely used in China, the ideal time to trigger ovulation is not yet well defined. Methods: This was a large-sample retrospective analysis. Between January 2016 and January 2020, 1,925 young patients who underwent their first in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles with normal ovarian response were divided into three groups based on their dominant follicular proportions (DFP, defined as ≥18 mm follicles /≥14 mm follicles; Group A: < 30%; Group B: 30% - 60%; Group C: ≥ 60%). Binary logistic regression and multivariate linear regression were used to assessed whether DFP levels were related to clinical pregnancy, number of blastocysts frozen, blastocyst formation rate and low blastocysts frozen. Results: Binary logistics regression analysis showed that compared with Group A, the OR for clinical pregnancy was 1.345 in Group B (P = 0.023); however, there was no statistical difference between Group C and Group A (P = 0.216). On one hand, multivariate linear regression analysis indicated that DFP was negatively associated with number of blastocysts frozen (β ± SE: Group B vs Group A = -0.319 ± 0.115, P = 0.006; Group C vs Group A = -0.432 ± 0.154, P = 0.005) as well as blastocyst formation rate (β ± SE: Group B vs Group A = -0.035 ± 0.016, P = 0.031; Group C vs Group A = -0.039 ± 0.021, P = 0.067). On the other hand, compared with group A, the OR for low blastocyst frozen was 1.312 in Group B (P = 0.039) and was 1.417 in Group C (P = 0.041). Conclusions: Excessive delay of trigger in GnRH-a prolonged protocol might reduce the developmental potential of oocytes and reduce the number of available blastocysts, which might result in a lower cumulative pregnancy rate. But further confirmation by strict prospective randomized controlled study should be needed.Trial registration: https://clinicaltrials.gov/; NCT03305510; Registered 08 October 2017 - Retrospectively registered.

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Effects of Gender of Reciprocal Chromosomal Translocation on Blastocyst Formation and Pregnancy Outcome in Preimplantation Genetic Testing

Frontiers in Endocrinology ◽

10.3389/fendo.2021.704299 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hui Song ◽

Hao Shi ◽

En-tong Yang ◽

Zhi-qin Bu ◽

Zi-qi Jin ◽

...

Keyword(s):

Genetic Testing ◽

Pregnancy Outcome ◽

Reciprocal Translocation ◽

Maternal Age ◽

Formation Rate ◽

Chromosomal Translocation ◽

Blastocyst Formation ◽

Preimplantation Genetic Testing ◽

Group A ◽

Group B

ObjectiveTo determine the effect of gender of reciprocal chromosomal translocation on blastocyst formation and pregnancy outcome in preimplantation genetic testing, including different parental ages.MethodsThis was a retrospective cohort study that enrolled 1034 couples undergoing preimplantation genetic testing-structural rearrangement on account of a carrier of reciprocal chromosomal translocation from the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2015 to December 2019. Group A represented 528 couples in which the man was the carrier of reciprocal translocation and group B represented 506 couples in which the woman was the carrier of reciprocal translocation. All patients were divided into two groups according to their age: female age<35 and female age≥35. Furthermore, the differences in blastocyst condition and pregnancy outcome between male and female carriers in each group were further explored according to their father’s age.ResultsThe blastocyst formation rate of group A (55.3%) is higher than that of group B (50%) and the results were statistically significant (P<0.05). The blastocyst formation rate of group A is higher than that of group B, no matter in young maternal age or in advanced maternal age (P<0.05). The blastocyst formation rate in maternal age<35y and paternal age<30y in group A(57.1%) is higher than that of Group B(50%); Similarly, the blastocyst formation rate in maternal age≥35 and paternal age≥38y(66.7%) is higher than that of Group B(33.3%)(all P<0.05). There was no difference in fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate between Group A and Group B.ConclusionWhen the carrier of reciprocal translocation is male, the blastocyst formation rate is higher than that of female carrier. While there is no significant difference between the two in terms of fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate.

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Analysis of the difference in characteristics of patients and IVF cycles and the parameters of in vitro embryo culture between normal individuals with high and low blastocyst formation rate

10.21203/rs.3.rs-17214/v1 ◽

2020 ◽

Author(s):

Chun-mei Yu ◽

Xiu-liang Dai ◽

yu-feng Wang ◽

Li Chen

Keyword(s):

Embryo Culture ◽

Formation Rate ◽

Ovarian Response ◽

Individual Characteristics ◽

Blastocyst Formation ◽

Significant Difference ◽

Group A ◽

The Difference ◽

Group B

Abstract Background: The main purpose of this study is to analyze the possible factors that were related with high useful blastocyst formatio rate in normal patients.Methods: This was a retrospective cohort study included 706 normal patients(excluding PCOS, DOR, endometriosis and other special diseases),according to the useful blastocyst in vitro formation rate(UBIVFR), the patients were divided into two groups respectively,group A（0-50%,including 50%）and group B（50%-100%). Results: There were no significant difference in patient ages between the two groups,compared with group A , the level of basic LH(bLH) was significantly increased. The clincal data showed that ,compared with group A ,the total Gn dose and the number of dominant follicles , oocytes retrieved，MII mature oocytes, normal fertilized oocytes rate,high-quality embryos rate and FET clinical pregnancy rate were significantly higher than group B.Conclusion:Our study is to investigate the useful blastocyst formation rate in relation to bLH levels in IVF/ICSI cycles, showing that high bLH is associated with higher ovarian response and higher UBIVFR.AMH,bFSH and AFC which were defined as ovarian reserve and ovarian response are seem not associated with the useful blastocyst formation. Key words:Useful blastocyst formation; normal patients; individual characteristics; basic LH; ovarian response; in vitro embryo culture

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Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI

Journal of Mammalian Ova Research ◽

10.1274/jmor.32.115 ◽

2015 ◽

Vol 32 (3) ◽

pp. 115-120

Author(s):

Masahiro Sakurai ◽

Shinichi Watanabe ◽

Toyomi Tanaka ◽

Rie Matsunaga ◽

Naoko Yamanaka ◽

...

Keyword(s):

Calcium Ionophore ◽

Oocyte Activation ◽

Artificial Oocyte Activation

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A positron emission tomography study of cerebrovascular reserve before and after shunt surgery in patients with idiopathic chronic hydrocephalus

Neurosurgical FOCUS ◽

10.3171/foc.2000.8.2.8 ◽

2000 ◽

Vol 8 (2) ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Petra M. Klinge ◽

Georg Berding ◽

Thomas Brinker ◽

Wolfram H. Knapp ◽

Madjid Samii

Keyword(s):

Positron Emission Tomography ◽

Clinical Outcome ◽

Emission Tomography ◽

Control Group ◽

Chronic Hydrocephalus ◽

Cerebrovascular Reserve ◽

Shunt Placement ◽

Positron Emission ◽

Group A ◽

Group B

Object In this study the authors use positron emission tomography (PET) to investigate cerebral blood flow (CBF) and cerebrovascular reserve (CVR) in chronic hydrocephalus. Methods Ten patients whose mean age was 67 ± 10 years (mean ± standard deviation [SD]) were compared with 10 healthy volunteers who were 25 ±3 years of age. Global CBF and CVR were determined using 15O–H2O and PET prior to shunt placement and 7 days and 7 months thereafter. The CVR was measured using 1 g acetazolamide. Neurological status was assessed based on a score assigned according to the methods of Stein and Langfitt. Seven months after shunt placement, five patients showed clinical improvement (Group A) and five did not (Group B). The average global CBF before shunt deployment was significantly reduced in comparison with the control group (40 ± 8 compared with 61 ± 7 ml/100 ml/minute; mean ± SD, p < 0.01). In Group A the CBF values were significantly lower than in Group B (36 ± 7 compared with 44 ± 8 ml/100 ml/minute; p < 0.05). The CVR before surgery, however, was not significantly different between groups (Group A = 43 ± 21%, Group B = 37 ± 29%). After shunt placement, there was an increase in the CVR in Group A to 52 ± 37% after 7 days and to 68 ± 47% after 7 months (p < 0.05), whereas in Group B the CVR decreased to 14 ± 18% (p < 0.05) after 7 days and returned to the preoperative level (39 ± 6%) 7 months after shunt placement. Conclusions The preliminary results indicate that a reduced baseline CBF before surgery does not indicate a poor prognosis. Baseline CBF before shunt placement and preoperative CVR are not predictive of clinical outcome. A decrease in the CVR early after shunt placement, however, is related to poor late clinical outcome, whereas early improvement in the CVR after shunt placement indicates a good prognosis.

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Microrna Expression Profile Identifies Distinct Clinically Relevant Sub-Groups in Multiple Myeloma: Novel Prognostic Markers and Potential Targets for Therapy

Blood ◽

10.1182/blood.v112.11.96.96 ◽

2008 ◽

Vol 112 (11) ◽

pp. 96-96 ◽

Cited By ~ 2

Author(s):

Sophia Adamia ◽

Herve AvetLoiseau ◽

Samirkumar B Amin ◽

Yu-Tzu Tai ◽

Steven P. Treon ◽

...

Keyword(s):

Multiple Myeloma ◽

Cell Differentiation ◽

Clinical Outcome ◽

Signaling Pathways ◽

Cell Lines ◽

Plasma Cells ◽

Normal Plasma ◽

Group A ◽

Group B

Abstract MicroRNA, an abundant class of small endogenous RNAs, regulate target genes through inducing translational inhibition and cleavage of targeted transcripts. To date, microRNAs have been implicated in normal biological processes, including development, cell differentiation, apoptosis and proliferation as well as in malignant transformation. However, their role in multiple myeloma (MM) remains unknown. Here we investigated role of microRNAs in myelomagenesis, and their influence on prognosis and clinical outcome. We evaluated profiles of 384 microRNAs in bone marrow derived CD138+ plasma cells (PC) from 79 uniformly treated MM patients, 11 MM cell lines and 9 healthy donors using qRT-PCR based microRNA array. The relative expression was calculated using comparative Ct method, and data was normalized using endogenous controls and analyzed using SDS, RQ manager, R and dChip softwares. MicroRNA expression profiles detected in MM patients were correlated with clinical outcome measures. We observed significant modulate expression of 61 microRNAs in myeloma cells compared to normal plasma cells. When more stringent criteria were used, we identified 24 differentially expressed microRNAs in patient samples. Further, unsupervised hierarchical clustering of filtered microRNAs, based on their DCt values, identified two major groups within the MM population (groups A and group B). Samples of Group A clusters with MM cell lines, indicating more proliferative nature of MM patient cells. Within B group, a second degree node group B2, clusters with normal plasma cells indicating more indolent course, while patients in an additional node B1 represented an assorted pattern. The unsupervised clustering of all MM samples showed consistent changes in miR-30b, -30c, -30d, -142-5p, -24, -191, -181d, -374, -146b, -140, -145, -125a, -151, -223, -155, let7b, indicative of a role of these microRNA in myelomagenesis; while supervised analysis of samples within groups A and B identified modulated expression of different sets of miRNAs. In group A miR-585 and let-7f were upregulated 8–12 fold, while miRs -125a, -126, -155, -223, -146a, -374 -19a, -20a, -26a, -30a -5p, -30b, and -30d were significantly downregulated; in group B, all differentially expressed microRNAs were downregulated (p<0.001) compared to normal plasma cells. These modulated miRNAs target critical signaling pathways including apoptosis, hematopoietic cell differentiation and proliferation, survival and angiogenesis by upregulating function of HOX9, c-myc, VCAM-1, Bcl-2, E2F1, SHP1, SHP2, VEGF, and DUSp6 molecules. We further analyzed the effect of microRNA on clinical outcome. We have observed significantly superior event free and overall survival of patients in group B2 compared to patients in group A (2 yr estimated EFS 79% versus 54% respectively; p=0.05; and 2 yr estimated OS 94% versus 70% respectively; p =0.017). Taken together this data identifies critical microRNAs as modulators of gene expression and signaling pathways and provides potential novel microRNA and gene targets in MM to both understand biological behavior and for therapeutic application.

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Artificial oocyte activation with CALCIUM IONOPHORE (A+23187) following ICSI fertilization failure

Fertility and Sterility ◽

10.1016/j.fertnstert.2015.07.945 ◽

2015 ◽

Vol 104 (3) ◽

pp. e302

Author(s):

A. Sdrigotti ◽

G.J. Rey Valzacchi ◽

F.A. Leocata Nieto ◽

V.E. Canada

Keyword(s):

Calcium Ionophore ◽

Oocyte Activation ◽

Fertilization Failure ◽

A 23187 ◽

Artificial Oocyte Activation

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Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-013-0110-2 ◽

2013 ◽

Vol 30 (12) ◽

pp. 1569-1575 ◽

Cited By ~ 12

Author(s):

Hye Jin Yoon ◽

In Hee Bae ◽

Hyoung Jun Kim ◽

Jung Mi Jang ◽

Yong Su Hur ◽

...

Keyword(s):

Clinical Outcomes ◽

Calcium Ionophore ◽

Oocyte Activation ◽

Artificial Oocyte Activation

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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype

10.21203/rs.2.14678/v2 ◽

2019 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Embryonic Development ◽

Formation Rate ◽

Early Embryonic Development ◽

High Quality ◽

Abnormal Embryo ◽

Molecular Karyotype ◽

Group A ◽

The Difference ◽

Group B ◽

Euploid Embryo

Abstract Background: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population. The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype. Results: Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrieved in the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization. After intracytoplasmic sperm injection, 181 oocytes normally fertilized.The rates of embryos forming on day3 in three groups were 87.88%, 97.78% and 77.14%, which was significantly different (P=0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C (P=0.01). Furthermore, the rates of high-quality blastocysts in three group were 14.71%, 48.15% and 62.96%, respectively, which was significantly different (P=0.00). Compared with group B and C, the rate of high-quality blastocysts in group A was statistically significantly lower (P=0.00; P=0.00). Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectoderm cells and 8 blastomeres. Except 7 embryos failed to amplify, 9.01% embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71% and the abnormal embryo rate was 89.29%. In group B, the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C. There were no significant differences among the three groups (P = 0.55). Conclusions: The double two-way translocations couples have more chance to get balanced or normal embryos probably, and there may be more high-quality blastocysts in exceptional CCRs, but the blastocyst formation rate was similar among the three type of BCCR. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic development and molecular karyotype should be further studied.

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