scholarly journals Antioxidant properties of aqueous media with molecular hydrogen used in tnviromental medicine

2019 ◽  
Vol 59 (8) ◽  
pp. 140-146
Author(s):  
Anatoly A. Lapin ◽  
◽  
Irek G. Garifullin ◽  
Valery N. Zelenkov ◽  
Sergey D. Filippov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Molecular Hydrogen ◽  
Oral Fluid ◽  
Synergistic Effects ◽  
Aqueous Media ◽  
Antioxidant Properties ◽  
Operating Conditions ◽  
Aquatic Systems ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

The article presents the results of the biotechnological study of aquatic environments with molecular hydrogen by antioxidant activity. Molecular hydrogen has selective antioxidant activity against dangerous free radicals. It has anti-inflammatory, anti-apoptotic, anti-allergic effects and stimulates energy metabolism. The problem is urgent nowadays because of the great interest to molecular hydrogen as an antioxidant over the past ten years, but its quantitative metabolic parameters have not been estimated yet. The total antioxidant activity of aquatic systems saturated by hydrogen have been studied using coulometric analysis. It was shown that their antioxidant activity of aquatic systems increase of 8-10 times depending on the operating conditions and time of the "H2 Inhaler" device and 3 times when using a mineral generator active Hydrogen "Aquaspectr Mineral". The effect of molecular hydrogen have been studied by changing the antioxidant status of the human body, which was determined by changing the total antioxidant activity of urine and oral fluid during inhalation of hydrogen and oral administration of hydrogen-saturated water. The synergistic effects has been revealed in determining the total antioxidant activity of the oral fluid in the range from 14.02 to 17.59% rel. and synergism/antagonism of urine in the range from 37.17 to 27.62% rel., compared with control measurements. The total antioxidant activity was determined using electro-generated bromine, the samples were analyzed on an Expert-006 coulometer (Econix-Expert LLC, Russia) according to our certified method.

scholarly journals Antioxidant properties of aqueous media. Part 3. Water research during the Clarias gariepinus farming in the closed containment aquaculture system

2019 ◽  
Vol 60 (10) ◽  
pp. 74-80
Author(s):  
Anatoly A. Lapin ◽  
◽  
Maria E. Gordeeva ◽  
Marina L. Kalaida ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Clarias Gariepinus ◽  
Antioxidant Properties ◽  
Food Plants ◽  
Phosphorus Compounds ◽  
Total Antioxidant Activity ◽  
Waste Products ◽  
Aquaculture System ◽  
Vital Functions ◽  
Total Antioxidant

The article presents the results of a biochemical study of the total antioxidant activity of water during the Clarias gariepinus farming in closed containment aquaculture system, including the dynamics without replacement. For the first time is shown an increasing the total antioxidant activity with the highest values in the lower part of the biological filter. According to the results of daily observation, the fish behavior was adequate until the end of the experiment, which was stopped with the beginning of their mass death (more than 20%). Researches have shown the promise of using the indicator of total antioxidant activity to assess the work of the closed containment aquaculture system for hydrobionts farming. The issues of reducing the cost of farmed fish by the closed containment aquaculture system using aquaponics, which distinguished among other technologies by the high quality of environmentally friendly food products while significantly saving water resources which is an important problem for closed containment aquaculture system are considered. Food plants feed on bacteria from the waste products of fish while they consume the necessary secretion products from water – chemicals (nitrogenous, potassium, phosphorus compounds), naturally purifying the water. Aquaponics simulates the natural whirlpool, using the natural processes of fish vital functions for plants nutrient medium. As food plants can be used Salicornia europaea and glycophyte Nasturtium officinale, which has a high productivity and high content of vitamins, iron and glycosides. Both plants are tested in the closed containment aquaculture system including human, increased degree of isolation.

scholarly journals CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF MEDICINAL EXTRACTS (SAPONARIA OF-FICINALIS L.)

2017 ◽  
pp. 137-143 ◽  
Author(s):  
Галина (Galina) Батоевна (Batoevna) Ендонова (Endonova) ◽  
Татьяна (Tat'jana) Петровна (Petrovna) Анцупова (Ancupova) ◽  
Сысыгма (Sysygma) Дашиевна (Dashievna) Жамсаранова (Zhamsaranova)
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Properties ◽  
Aqueous Extracts ◽  
Total Antioxidant Activity ◽  
Chelating Activity ◽  
Total Antioxidant ◽  
The Republic ◽  
The Village ◽  
Saponaria Officinalis

The paper presents new data on chemical composition and antioxidant properties in extracts of the aerial organs of Saponaria officinalis growing on the territory of the Republic of Buryatia. It was revealed that soapberry leaves extracts contain a number of organic acids such as oxalic, formic, fumaric, succinic, malic, citric, and benzoic ones, the dominant being formic, malic, acetic and citric acids. Of vitamins B2, B3. BC extracted from the plant the dominant one was riboflavin. The method of and Fthigh-performance liquid chromatography helped to reveal five compounds identified as aglycone and glycosidic in character. They are orientin, isoeryentin, isovitinexin-7-О-glycoside, orientin-7-О-glycoside, of which isovitoxin is dominant. The greatest total antioxidant activity was demonstrated by soapberry leaves and inflorescences picked up on abandoned sites of the village of Selenginsk. Extractions from Saponaria officinalis leaves showed showed antiradical and Fe2+-chelating activity comparable to that of the extractions from plants with pronounced antioxidant activity. Thus, the data obtained indicated the antioxidant activity of S.officinalis aqueous extracts due to the presence of natural flavonoids. This fact shows the plant as a promising new source of antioxidants.

scholarly journals Effect of ultraviolet radiation on plant biochemical properties

2020 ◽  
Vol 63 (8) ◽  
pp. 134-140
Author(s):  
Valery N. Zelenkov ◽  
◽  
Anatoly A. Lapin ◽  
Vyacheslav V. Latushkin ◽  
Vladimir. V. Karpachev ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sugar Beet ◽  
Ultraviolet Radiation ◽  
Antioxidant Properties ◽  
Biochemical Properties ◽  
Ultrastructural Changes ◽  
Maximum Effect ◽  
Antioxidant Systems ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Depletion of the ozone layer leads to increased ultraviolet radiation, which affects the growth and functioning of plants and leads to their various physiological, biochemical, morphological and ultrastructural changes. When studying the effect of ultraviolet radiation on seed sowing qualities and biometric indicators of morphological organs of plants of various cultures, scientists did not come to a consensus on the optimal parameters of its effects. For seeds of each plant variety, there is an optimal amount of energy absorbed, resulting in a maximum effect. Ecologically significant low ultraviolet radiation changes the metabolism of reactive oxygen species and plant antioxidant systems by increasing enzyme regulation. The relevance of studies in this direction is obvious, as it allows you to stimulate the germination of seeds with physical influences, increasing their laboratory and field germination. The authors obtained and published new data on comparison of sown, crop and antioxidant properties of seeds and seedlings, seed viability, microzelenium biomass formation and change of total antioxidant activity of vegetable crops after thermodehydration. As a continuation of these studies, the purpose of this work was to study the effect of ultraviolet radiation on the biochemical properties of plants. The total antioxidant activity of sugar beet and nougat sprouts in the experiment increased under the influence of stress ultraviolet radiation. Compared to control samples (germination according to GOST in the dark), under the influence of ultraviolet radiation, antioxidant activity increases by 11.4-17.4% otn. Ultraviolet radiation is damaging factors of plant growth and development, which is manifested at different stages of ontogenesis. Thus, sowing properties are reduced (germination of sugar beet seeds is less than control by 18%, in nougat seeds by 12%), above-ground biomass at the end of the germination period is less by 49.0% in sugar beet and by 16.5% in Abyssinian nougat. Resistance to the damaging effects of ultraviolet radiation depends on the genetic nature of the plant: in the experiment, the sprouts of the Abyssinian nuga turned out to be more resistant.

scholarly journals Comparison of the sowing, harvesting and antioxidant properties of seeds and seedlings of a giant field moth (Agrostis gigantea Roth) after air drying and thermal dehydration

2020 ◽  
Vol 61 (3) ◽  
pp. 120-125
Author(s):  
Valery N. Zelenkov ◽  
◽  
Vyacheslav V. Lаtushkin ◽  
Аnatoly А. Lapin ◽  
Vladimir M. Kosolapov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Study ◽  
Antioxidant Properties ◽  
Thermal Dehydration ◽  
Total Antioxidant Activity ◽  
Time Data ◽  
Air Drying ◽  
Constant Weight ◽  
Feed Production ◽  
Total Antioxidant

The study of the biological characteristics of feed crops and the use of modern methods in the study of the processes of their selection is relevant for the intensification of feed production. The article presents the results of a biochemical study of the giant VIK-2 (Agrostis gigantea Roth) field birch seed, their sowing and yield properties after thermal dehydration (drying of air-dried samples at 105 oС to constant weight), as well as their total antioxidant activity during germination in the dark. The total antioxidant activity of seeds and seedlings was studied using coulometric analysis and determined using electrogenerated bromine, the samples were analyzed on a certified couponometer Expert-006 (LLC Econix-Expert, Russia) using a certified method. The work shows that, compared with air drying after thermohydration, there is a decrease in the viability and yield properties of seeds. Seed germination decreased by 5.5%, the height of plants at the end of the germination period (on the 14th day after sowing) by 9.4%, the mass of 100 sprouts – by 21.3%, the yield of green mass (microgreen) also by 21.3%. The antioxidant activity of seeds after thermal dehydration also decreased by 21.4%. For the first time, data were obtained on the viability, formation of microgreen biomass and a change in the total antioxidant activity of the seeds of the VIK-2 giant cultivar after thermal dehydration (drying the initial seeds) in the test at 105 °C to constant weight. It was shown that thermal dehydration of seeds at 105 °C significantly affects the viability of seeds and seedlings of a bent field, which is associated with the characteristics of metabolism, forms and state of water activity in cells and tissues of plants.

scholarly journals Antioxidant potentials of different potato genotypes

2018 ◽  
Vol 43 (3) ◽  
pp. 369-381
Author(s):  
SMZ Al Meraj ◽  
TK Ghosh ◽  
AKMA Islam ◽  
M Mohi Ud Din
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Food Security ◽  
Significant Variation ◽  
Antioxidant Properties ◽  
Total Content ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Total Carotenoids ◽  
Total Antioxidant

The present investigation was undertaken to analyze the antioxidant potential of sixteen different potato genotypes. Eleven yellow fleshed potato genotypes namely Forza, Courage, Laura, Rosa Gold, Lady Rosetta, Cumbica, Asterix, Coronada, Granola, Cardinal and Diamant and five purple fleshed potato genotypes namely Jam Alu, KAC 10063, KAC 10064, KAC 10069, KAC 10097 were used as experimental materials. Total carotenoids, anthocyanin, phenolics, flavonoids, ascorbic acid and antioxidant activity in the above mentioned genotypes were determined to compare the antioxidant potentials of the genotypes. Analysis of above mentioned parameters resulted significant variation in their contents in both yellow and purple fleshed genotypes. The results indicate the purple fleshed genotypes showed significantly higher carotenoids, anthocyanin, flavonoids and total antioxidant activity than those of yellow fleshed genotypes. The findings also suggested the antioxidant activity of the genotypes positively correlates to the total content of carotenoids, anthocyanin and flavonoids. Since, purple fleshed genotypes showed higher antioxidant properties, the results of this study claim that the purple fleshed genotypes would be suitable for direct consumption as vegetables which might increase the health and food security of human beings.Bangladesh J. Agril. Res. 43(3): 369-381, June 2018

scholarly journals Fruit of raspberry Rubus Idaeus L. as a source of functional ingredients (review)

2019 ◽  
Vol 48 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Екатерина Жбанова ◽  
Ekaterina Zhbanova
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Rubus Idaeus ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Red Raspberry ◽  
Total Anthocyanin ◽  
Wide Range ◽  
Total Antioxidant

The article gives a comparative analysis of the data obtained by Russian and foreign scientists considering the content of vitamins and other valuable substances in raspberry fruit. That determines high antioxidant properties of raspberry. The goal of the study was to generalize information which shows specific nature of that plant in relation to the complex of vitamins and polyphenol compounds which it contains. The author shows that fruit of raspberry R. idaeus L. consists of 5–40 mg/100 g of vitamin C, folic acid - 26-44 mg/100 g. Consumption of only 100 g of raspberries satisfies daily requirement of vitamin C (60 mg) by 8.3 - 66.7%, in folates (200 mg) by 13–22%. Total anthocyanin content in red raspberry varies within wide range (20–100 mg/100 g), in black raspberry – form 200 to 600 mg/100 g. Raspberry accumulates from 38 to 270 mg/100 g of ellagic acid. In small amounts the cultivated red raspberry consists of the following substances: carotenoids (β-carotene – 9.3 mg/100 g, zeaxanthin – 11 mg/100 g, lutein – 320 mg /100 g), vitamin E – 0.15–0.44 mg/100 g tocopherol equivalents, thiamine – 0,020 mg/100 g, riboflavin – 0.034 mg/100 g, niacin – 0.036 mg/100 g, pyridoxin – 0.05 mg/100 g, biotin – 5.7 mg/100 g. Total content of antioxidants in the cultivated raspberry fruit is 1.71 mg/g (standard quercetin). As a result of the analysis of different literary sources the author determined that raspberry antioxidant properties are mainly connected with high polyphenol content. Contribution of vitamin C into total antioxidant activity is relatively low. Though raspberry fruit chemical composition has already been studied quite well it is necessary to perform further more detailed research of different raspberry cultivars considering their total antioxidant activity as well as certain biochemical components which comprise antioxidant complex of raspberry fruit. The obtained results will form the base for further research aimed at investigating phytochemical compounds of fruit crops which have become an essential part of healthy human diet and developing nutraceutical products.

scholarly journals Justification for the use of “Aquaspectrum” containers for saturation of aqueous media with active hydrogen using a biochemical indicator of total antioxidant activity

2020 ◽  
Vol 61 (3) ◽  
pp. 59-65
Author(s):  
Anatoly A. Lapin ◽  
◽  
Ashot P. Khachatryan ◽  
Keyword(s):  
Antioxidant Activity ◽  
Alternative Energy ◽  
Biochemical Study ◽  
Aqueous Media ◽  
Operating Time ◽  
Volume Ratio ◽  
Total Antioxidant Activity ◽  
Technical Problems ◽  
Hydrogen Saturation ◽  
Total Antioxidant

The article presents the results of a biochemical study of aqueous environments with molecular hydrogen by antioxidant activity. Activated hydrogen has selective antioxidant activity against dangerous free radicals for the human body and animals. In addition, its anti-inflammatory, anti-apoptotic, anti-allergic properties were found and it stimulates energy metabolism in cells. This problem is currently relevant due to the great interest in activated hydrogen as an antioxidant over the past ten years, but its quantitative metabolic parameters have not yet been assessed at a sufficient scientific level. The total antioxidant activity of aqueous systems saturated with hydrogen was studied using coulometric analysis. It has been shown that their total antioxidant activity increases depending on the quality of aqueous media and the operating time of the “Aquaspectr Hydrogen Mug Mug for Activating Water” device: for Arkhyz mineral water, 20 times after 26 minutes of hydrogen saturation, drinking natural oxalis Kristalia with mineral drinking medicinal water DonatMg (in volume ratio) 31 times after 55 minutes and DonatMg mineral drinking medical water 44 times after 28 minutes. The total antioxidant activity was determined using electrically generated bromine, the samples were analyzed on a certified Expert-006 coulometer (Econix-Expert LLC, Russia) according to a certified method. In the work it was shown that saturation of water systems, in particular mineral and drinking water, by the method of hydrogen evolution on the surface of containers from environmentally friendly magnesium alloys, can be quite an effective way to activate various liquid media with antioxidants used in environmental medicine. In addition, our results can be useful in solving scientific and technical problems in the development of alternative energy sources.

scholarly journals Estimation of antioxidant properties of experimental poly-component complexes

2020 ◽  
Vol 82 (3) ◽  
pp. 63-66
Author(s):  
N. Raksha ◽  
Ju. Sokolovskaya ◽  
E. Manzhaliy ◽  
D. Dobryanskiy ◽  
O. Savchuk
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Superoxide Anion ◽  
Antioxidant Properties ◽  
General Mechanism ◽  
Scavenging Activity ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

The involvement of free radicals appears to be the feature of most human diseases. The general mechanism of cell damage involves the excessive uncontrolled production of reactive oxygen species resulting in the intensification of lipid peroxidation processes and damaging of macromolecules. These processes are generally accompanied by a decrease in the concentration and/or activity of natural antioxidants. Moreover, the exhaustion of the antioxidant capacity of the organism is among the key reasons leading to the development of pathological states. The maintenance of the prooxidant-antioxidant balance by the systematic use of products or dietary supplements, the components of which exhibit antioxidant activity, can be considered as a part of a strategy for the prevention and control of diseases associated with oxidative stress. The current work aims to study the free radical scavenging activity of two experimental poly-component complexes. Antioxidant properties of complexes were determined in experiments in vitro. The estimation of total antioxidant activity, nitric oxide, hydrogen peroxide radical, and superoxide anion scavenging activity was performed. The results revealed that both complexes have the potentials to prevent the formation of free radicals and can be used as agents with antioxidant properties. It was established that the effect of complex 1 and 2 is complex and first of all aimed at the prevention of the formation of dangerous free radicals by reducing the level of hydrogen peroxide. The additional mechanism involves the chelating of metal ions, in particular ferrous iron. The antioxidant ability exhibited by complex 1 was found to be higher than the total antioxidant activity of complex 2. Complex 1 was more effective in the ability to neutralize superoxide anion-radicals while complex 2 showed a high percentage inhibition of Fe2+-induced lipid peroxidation.

scholarly journals PORTABLE AMPEROMETRIC SENSOR FOR MEASURING THE TOTAL ANTIOXIDANT ACTIVITY OF SUBSTANCES

2021 ◽  
Vol 10 (5) ◽  
pp. 13-22
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Indicator Electrode ◽  
Measured Signal ◽  
Total Antioxidant Activity ◽  
Depolarization Current ◽  
Measuring Device ◽  
Amperometric Method ◽  
Total Antioxidant

Understanding the role of antioxidants as substances interrupting free radical reactions intensifies the search for new natural and synthetic substances with antioxidant properties. Along with this, intensive research is underway to create methods for assessing the antioxidant (AO) properties of various substances and devices for determining antioxidant activity (AOA). This article proposes an amperometric method and a sensor for measuring the total antioxidant activity of substances. The method is based on the process of oxygen electroreduction in the absence and presence of antioxidants of various origins in the solution. The measurement of the analytical signal before and after the introduction of the antioxidant substance into the electrochemical cell is carried out using a portable two-electrode sensor, "pencil" type, with a silver cathode, on which a potential of -500 mV is applied from a DC source (batteries). As an antioxidant standard, a 1% solution of ascorbic acid in a phosphate buffer with a pH of 6.86 was conventionally adopted. The sensor is connected to the measuring and driving device through a microcircuit - an operational amplifier. The measured signal in arbitrary units is displayed on the liquid crystal screen of the setting and measuring device and characterizes the maximum depolarization current of the indicator electrode. Using the resistors of the measuring device, a range of 0 - 100 units is set, for the analyte in the absence of an antioxidant and in the presence of a standard - 1% ascorbic acid solution, conventionally taken as 100%, respectively. Thus, the antioxidant activity of substances can be measured as a percentage relative to ascorbic acid (or other antioxidant). The results of determining the total antioxidant activity of some drinks and aqueous-alcoholic extracts of medicinal plants are presented.

scholarly journals Effect of dark chocolate on plasma epicatechin levels, DNA resistance to oxidative stress and total antioxidant activity in healthy subjects

2009 ◽  
Vol 103 (7) ◽  
pp. 1008-1014 ◽  
Author(s):  
A. Spadafranca ◽  
C. Martinez Conesa ◽  
S. Sirini ◽  
G. Testolin
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Healthy Subjects ◽  
Antioxidant Properties ◽  
Experimental Period ◽  
Dark Chocolate ◽  
Total Antioxidant Activity ◽  
Dna Damages ◽  
Balanced Diet ◽  
Total Antioxidant

Dark chocolate (DC) may be cardioprotective by antioxidant properties of flavonoids. We investigated the effect of DC (860 mg polyphenols, of which 58 mg epicatechin) compared with white chocolate (WC; 5 mg polyphenols, undetectable epicatechin) on plasma epicatechin levels, mononuclear blood cells (MNBC) DNA damage and plasma total antioxidant activity (TAA). Twenty healthy subjects followed a balanced diet (55 % of energy from carbohydrates, 30 % from fat and 1 g protein/kg body weight) for 4 weeks. Since the 14th day until the 27th day, they introduced daily 45 g of either WC (n 10) or DC (n 10). Whole experimental period was standardised in antioxidant intake. Blood samples were collected at T0, after 2 weeks (T14), 2 h and 22 h after the first chocolate intake (T14+2 h and T14+22 h), and at 27th day, before chocolate intake (T27), 2 h and 22 h after (T27+2 h and T27+22 h). Samples, except for T14+2 h and T27+2 h, were fasting collected. Detectable epicatechin levels were observed exclusively 2 h after DC intake (T14+2 h = 0·362 (se 0·052) μmol/l and T27+2 h = 0·369 (se 0·041) μmol/l); at the same times corresponded lower MNBC DNA damages (T14+2 h = − 19·4 (se 3·4) % v. T14, P < 0·05; T27+2 h = − 24 (se 7·4) % v. T27, P < 0·05; T14+2 hv. T27+2 h, P = 0·7). Both effects were no longer evident after 22 h. No effect was observed on TAA. WC did not affect any variable. DC may transiently improve DNA resistance to oxidative stress, probably for flavonoid kinetics.

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