scholarly journals Comparison of the sowing, harvesting and antioxidant properties of seeds and seedlings of a giant field moth (Agrostis gigantea Roth) after air drying and thermal dehydration

2020 ◽  
Vol 61 (3) ◽  
pp. 120-125
Author(s):  
Valery N. Zelenkov ◽  
◽  
Vyacheslav V. Lаtushkin ◽  
Аnatoly А. Lapin ◽  
Vladimir M. Kosolapov ◽  
...  

The study of the biological characteristics of feed crops and the use of modern methods in the study of the processes of their selection is relevant for the intensification of feed production. The article presents the results of a biochemical study of the giant VIK-2 (Agrostis gigantea Roth) field birch seed, their sowing and yield properties after thermal dehydration (drying of air-dried samples at 105 oС to constant weight), as well as their total antioxidant activity during germination in the dark. The total antioxidant activity of seeds and seedlings was studied using coulometric analysis and determined using electrogenerated bromine, the samples were analyzed on a certified couponometer Expert-006 (LLC Econix-Expert, Russia) using a certified method. The work shows that, compared with air drying after thermohydration, there is a decrease in the viability and yield properties of seeds. Seed germination decreased by 5.5%, the height of plants at the end of the germination period (on the 14th day after sowing) by 9.4%, the mass of 100 sprouts – by 21.3%, the yield of green mass (microgreen) also by 21.3%. The antioxidant activity of seeds after thermal dehydration also decreased by 21.4%. For the first time, data were obtained on the viability, formation of microgreen biomass and a change in the total antioxidant activity of the seeds of the VIK-2 giant cultivar after thermal dehydration (drying the initial seeds) in the test at 105 °C to constant weight. It was shown that thermal dehydration of seeds at 105 °C significantly affects the viability of seeds and seedlings of a bent field, which is associated with the characteristics of metabolism, forms and state of water activity in cells and tissues of plants.

2019 ◽  
Vol 59 (7) ◽  
pp. 134-141
Author(s):  
Anatoly A. Lapin ◽  
◽  
Irek G. Garifullin ◽  
Valery N. Zelenkov ◽  
◽  
...  

The article presents the results of biochemical studies of the total antioxidant activity of the hepatoprotective collection of plant origin and its individual components, which show that it consists of birch leaves (Betulae folia), tansy flowers (Tanaceti flores), phytohepatol No. 2 (choleretic collection No. 2) and individual its components have pronounced antioxidant activity. More antioxidant activity founded in tansy and true hepatoprotective collection. Comparative tests of the flowers of meadowsweet, with the highest antioxidant activity, showed the promise of their use in the collections of medicinal plants as effective natural metabolic regulators. The total antioxidant activity of the water extract collection at 13.56% rel. exceeded the calculated value obtained by summing the activities of the individual components of the collection, which indicates the presence of synergism in their action, which may be of value for use in the treatment and prevention of liver diseases. When all components of the drug collection are finished, the indicator increases by 11-24 % rel., which indicates the thermal stability of the antioxidant substances that make up their composition. In the oxidation of aqueous extracts of the components of hepatoprotective collection with hydrogen peroxide, the most stable were antioxidants of birch leaves. It was found that collection only at a ratio of 7.7 g per liter of water shows synergism (15 % rel.), and the rest of all the samples we tested are antagonistic. The detected increase in the antioxidant activity of the new collection compared with the effects of its individual components allows us to consider it as a promising dosage form for use in the treatment and prevention of liver diseases.


2019 ◽  
Vol 60 (10) ◽  
pp. 74-80
Author(s):  
Anatoly A. Lapin ◽  
◽  
Maria E. Gordeeva ◽  
Marina L. Kalaida ◽  
◽  
...  

The article presents the results of a biochemical study of the total antioxidant activity of water during the Clarias gariepinus farming in closed containment aquaculture system, including the dynamics without replacement. For the first time is shown an increasing the total antioxidant activity with the highest values in the lower part of the biological filter. According to the results of daily observation, the fish behavior was adequate until the end of the experiment, which was stopped with the beginning of their mass death (more than 20%). Researches have shown the promise of using the indicator of total antioxidant activity to assess the work of the closed containment aquaculture system for hydrobionts farming. The issues of reducing the cost of farmed fish by the closed containment aquaculture system using aquaponics, which distinguished among other technologies by the high quality of environmentally friendly food products while significantly saving water resources which is an important problem for closed containment aquaculture system are considered. Food plants feed on bacteria from the waste products of fish while they consume the necessary secretion products from water – chemicals (nitrogenous, potassium, phosphorus compounds), naturally purifying the water. Aquaponics simulates the natural whirlpool, using the natural processes of fish vital functions for plants nutrient medium. As food plants can be used Salicornia europaea and glycophyte Nasturtium officinale, which has a high productivity and high content of vitamins, iron and glycosides. Both plants are tested in the closed containment aquaculture system including human, increased degree of isolation.


2017 ◽  
pp. 137-143 ◽  
Author(s):  
Галина (Galina) Батоевна (Batoevna) Ендонова (Endonova) ◽  
Татьяна (Tat'jana) Петровна (Petrovna) Анцупова (Ancupova) ◽  
Сысыгма (Sysygma) Дашиевна (Dashievna) Жамсаранова (Zhamsaranova)

The paper presents new data on chemical composition and antioxidant properties in extracts of the aerial organs of Saponaria officinalis growing on the territory of the Republic of Buryatia. It was revealed that soapberry leaves extracts contain a number of organic acids such as oxalic, formic, fumaric, succinic, malic, citric, and benzoic ones, the dominant being formic, malic, acetic and citric acids. Of vitamins B2, B3. BC extracted from the plant the dominant one was riboflavin. The method of and Fthigh-performance liquid chromatography helped to reveal five compounds identified as aglycone and glycosidic in character. They are orientin, isoeryentin, isovitinexin-7-О-glycoside, orientin-7-О-glycoside, of which isovitoxin is dominant. The greatest total antioxidant activity was demonstrated by soapberry leaves and inflorescences picked up on abandoned sites of the village of Selenginsk. Extractions from Saponaria officinalis leaves showed showed antiradical and Fe2+-chelating activity comparable to that of the extractions from plants with pronounced antioxidant activity. Thus, the data obtained indicated the antioxidant activity of S.officinalis aqueous extracts due to the presence of natural flavonoids. This fact shows the plant as a promising new source of antioxidants.


2019 ◽  
Vol 60 (10) ◽  
pp. 60-66
Author(s):  
Ekaterina V. Ferubko ◽  
◽  
Valery N. Zelenkov ◽  
Tamara D. Dergaeva ◽  
Anatoly A. Lapin ◽  
...  

At present, the expansion of research to find sources for obtaining new effective and safe medicines of plant origin, including those used in gastroenterological practice, is a pressing task of medical science, considering that the range of medicinal plant drugs used in practical health care is more than 40%. The article presents the results of biochemical study of total antioxidant activity of anti-ulcer collection of plant origin and its individual components. We show that it consists of the leaves of the planter large (Plantago major L.), grass of a sushchenitsa uliginose (Gnaphalium uliginosum L.), rhizomes and roots of the devisil high (Inula helenium L.), flowers of the chamomile pharmacy (Matricaria chamomilla L.), the roots of licorice naked (Glycyrrhiza glabra L.), grass of bird's mountain (Polygonum aviculare L.), leaves of the nettle (Urtica dioica L.), fruits of a mountain ash ordinary (Sorbus aucuparia L.) and its individual components have pronounced antioxidant activity. The greatest activity was found in the roots of licorice naked, grass of bird 's mountain and flowers of chamomile pharmacy. Total antioxidant activity of water extraction of collection recovery at 6.34% from exceeded the calculated value obtained by summing the activities of the individual components of the collection, which indicates the presence of synergy in their action, which may be of importance for use in the treatment and prevention of diseases in gastroenterological practice. The detected increase in the antioxidant activity of the new collection compared to the effects of its individual components allows it to be considered as a promising dosage form for the treatment and prevention of gastroenterological diseases.


2020 ◽  
Vol 61 (1) ◽  
pp. 118-125
Author(s):  
Anatoly A. Lapin ◽  
◽  
Marina L. Kalaida ◽  
Valery N. Zelenkov ◽  
◽  
...  

Expanding research to find sources for new effective and environmentally friendly fish feed additives based on natural plant-based raw materials is a pressing agricultural concern Biotechnology. This is especially true because feed additives for fish farming, as opposed to the use of feed additives for farm animals, come into contact with the environment of reservoirs and, accordingly, can have a significant impact on the ecosystem water environment for all inhabitants of reservoirs for breeding fish as aquaculture. The article presents the results of a biochemical study of the total antioxidant activity of the developed feed additives based on the components of the grain of spring wheat (Triticum) of the Kazan Jubilee and amaranth herbs (Amaranthus cruentus L) Shuntuk varieties from the collection of the botanical garden of the All-Russian Research Institute of Medicinal and Aromatic Plants. We show that when applying the technological equipment for grinding the original components for the preparation of feed additives, the total antioxidant activity of the wheat and amaranth grain fractions after grinding depends on the degree of the grinding or from the variance of component particles after sowing on the Sith. It is shown that when preparing fish feed it is best to use wheat with the size of particles 0.25-0.4 mm, which corresponds to the maximum manifestation of the total antioxidant activity of the crushed grain. For the first time in the experiment revealed a synergistic effect on the manifestation of total antioxidant activity when adding protein-vitamin flour from dry amaranth herb to the crushed wheat grain with a maximum value of 4% of its content from the total mass Feed.


2020 ◽  
Vol 63 (8) ◽  
pp. 134-140
Author(s):  
Valery N. Zelenkov ◽  
◽  
Anatoly A. Lapin ◽  
Vyacheslav V. Latushkin ◽  
Vladimir. V. Karpachev ◽  
...  

Depletion of the ozone layer leads to increased ultraviolet radiation, which affects the growth and functioning of plants and leads to their various physiological, biochemical, morphological and ultrastructural changes. When studying the effect of ultraviolet radiation on seed sowing qualities and biometric indicators of morphological organs of plants of various cultures, scientists did not come to a consensus on the optimal parameters of its effects. For seeds of each plant variety, there is an optimal amount of energy absorbed, resulting in a maximum effect. Ecologically significant low ultraviolet radiation changes the metabolism of reactive oxygen species and plant antioxidant systems by increasing enzyme regulation. The relevance of studies in this direction is obvious, as it allows you to stimulate the germination of seeds with physical influences, increasing their laboratory and field germination. The authors obtained and published new data on comparison of sown, crop and antioxidant properties of seeds and seedlings, seed viability, microzelenium biomass formation and change of total antioxidant activity of vegetable crops after thermodehydration. As a continuation of these studies, the purpose of this work was to study the effect of ultraviolet radiation on the biochemical properties of plants. The total antioxidant activity of sugar beet and nougat sprouts in the experiment increased under the influence of stress ultraviolet radiation. Compared to control samples (germination according to GOST in the dark), under the influence of ultraviolet radiation, antioxidant activity increases by 11.4-17.4% otn. Ultraviolet radiation is damaging factors of plant growth and development, which is manifested at different stages of ontogenesis. Thus, sowing properties are reduced (germination of sugar beet seeds is less than control by 18%, in nougat seeds by 12%), above-ground biomass at the end of the germination period is less by 49.0% in sugar beet and by 16.5% in Abyssinian nougat. Resistance to the damaging effects of ultraviolet radiation depends on the genetic nature of the plant: in the experiment, the sprouts of the Abyssinian nuga turned out to be more resistant.


2019 ◽  
Vol 59 (8) ◽  
pp. 140-146
Author(s):  
Anatoly A. Lapin ◽  
◽  
Irek G. Garifullin ◽  
Valery N. Zelenkov ◽  
Sergey D. Filippov ◽  
...  

The article presents the results of the biotechnological study of aquatic environments with molecular hydrogen by antioxidant activity. Molecular hydrogen has selective antioxidant activity against dangerous free radicals. It has anti-inflammatory, anti-apoptotic, anti-allergic effects and stimulates energy metabolism. The problem is urgent nowadays because of the great interest to molecular hydrogen as an antioxidant over the past ten years, but its quantitative metabolic parameters have not been estimated yet. The total antioxidant activity of aquatic systems saturated by hydrogen have been studied using coulometric analysis. It was shown that their antioxidant activity of aquatic systems increase of 8-10 times depending on the operating conditions and time of the "H2 Inhaler" device and 3 times when using a mineral generator active Hydrogen "Aquaspectr Mineral". The effect of molecular hydrogen have been studied by changing the antioxidant status of the human body, which was determined by changing the total antioxidant activity of urine and oral fluid during inhalation of hydrogen and oral administration of hydrogen-saturated water. The synergistic effects has been revealed in determining the total antioxidant activity of the oral fluid in the range from 14.02 to 17.59% rel. and synergism/antagonism of urine in the range from 37.17 to 27.62% rel., compared with control measurements. The total antioxidant activity was determined using electro-generated bromine, the samples were analyzed on an Expert-006 coulometer (Econix-Expert LLC, Russia) according to our certified method.


2020 ◽  
Vol 62 (6) ◽  
pp. 131-137
Author(s):  
Valery N. Zelenkov ◽  

The study examined germination of seeds, antioxidant properties of seeds, sprouts and biomass of four vegetable crops under controlled conditions under various modes of seed drying. After thermal dehydration of the seeds (drying of samples at 105 °C), a sharp decrease in sowing properties occurs compared to air drying. Thermal dehydration strongly affects the viability of seeds and plants, but the level of adaptation to this stress effect is genetically determined and associated with metabolic features, forms and the state of water in plant cells and tissues. So, coriander seeds completely lose germination, and chicory seeds germinate quite well. New data are obtained on seed viability, microzelenium biomass formation and change of total antioxidant activity of studied vegetable crops under conditions of increased temperatures. In addition to chicory, carrot seeds showed some resistance to thermal dehydration. The height of the sprouts in chicory in experimental and control versions is close, while in carrots they differ almost 9 times. The yield of microselenium of carrots decreased by 11.5 times with the use of seeds subjected to thermal dehydration, the yield of chicory microselenium – by 2.7 times. The antioxy-data activity of coriander seeds, carrots and pasternaci decreases after thermodehydration, chicory seeds practically do not change. The antioxidant activity of sprouts is several times higher than that of seeds, which is characteristic of all studied crops. The formation of microzelenium during dark selection lasts no more than 2 weeks from the moment of sowing, obviously, therefore, it is impractical to grow microzelenium in the dark longer than this period.


2018 ◽  
Vol 43 (3) ◽  
pp. 369-381
Author(s):  
SMZ Al Meraj ◽  
TK Ghosh ◽  
AKMA Islam ◽  
M Mohi Ud Din

The present investigation was undertaken to analyze the antioxidant potential of sixteen different potato genotypes. Eleven yellow fleshed potato genotypes namely Forza, Courage, Laura, Rosa Gold, Lady Rosetta, Cumbica, Asterix, Coronada, Granola, Cardinal and Diamant and five purple fleshed potato genotypes namely Jam Alu, KAC 10063, KAC 10064, KAC 10069, KAC 10097 were used as experimental materials. Total carotenoids, anthocyanin, phenolics, flavonoids, ascorbic acid and antioxidant activity in the above mentioned genotypes were determined to compare the antioxidant potentials of the genotypes. Analysis of above mentioned parameters resulted significant variation in their contents in both yellow and purple fleshed genotypes. The results indicate the purple fleshed genotypes showed significantly higher carotenoids, anthocyanin, flavonoids and total antioxidant activity than those of yellow fleshed genotypes. The findings also suggested the antioxidant activity of the genotypes positively correlates to the total content of carotenoids, anthocyanin and flavonoids. Since, purple fleshed genotypes showed higher antioxidant properties, the results of this study claim that the purple fleshed genotypes would be suitable for direct consumption as vegetables which might increase the health and food security of human beings.Bangladesh J. Agril. Res. 43(3): 369-381, June 2018


2019 ◽  
Vol 48 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Екатерина Жбанова ◽  
Ekaterina Zhbanova

The article gives a comparative analysis of the data obtained by Russian and foreign scientists considering the content of vitamins and other valuable substances in raspberry fruit. That determines high antioxidant properties of raspberry. The goal of the study was to generalize information which shows specific nature of that plant in relation to the complex of vitamins and polyphenol compounds which it contains. The author shows that fruit of raspberry R. idaeus L. consists of 5–40 mg/100 g of vitamin C, folic acid - 26-44 mg/100 g. Consumption of only 100 g of raspberries satisfies daily requirement of vitamin C (60 mg) by 8.3 - 66.7%, in folates (200 mg) by 13–22%. Total anthocyanin content in red raspberry varies within wide range (20–100 mg/100 g), in black raspberry – form 200 to 600 mg/100 g. Raspberry accumulates from 38 to 270 mg/100 g of ellagic acid. In small amounts the cultivated red raspberry consists of the following substances: carotenoids (β-carotene – 9.3 mg/100 g, zeaxanthin – 11 mg/100 g, lutein – 320 mg /100 g), vitamin E – 0.15–0.44 mg/100 g tocopherol equivalents, thiamine – 0,020 mg/100 g, riboflavin – 0.034 mg/100 g, niacin – 0.036 mg/100 g, pyridoxin – 0.05 mg/100 g, biotin – 5.7 mg/100 g. Total content of antioxidants in the cultivated raspberry fruit is 1.71 mg/g (standard quercetin). As a result of the analysis of different literary sources the author determined that raspberry antioxidant properties are mainly connected with high polyphenol content. Contribution of vitamin C into total antioxidant activity is relatively low. Though raspberry fruit chemical composition has already been studied quite well it is necessary to perform further more detailed research of different raspberry cultivars considering their total antioxidant activity as well as certain biochemical components which comprise antioxidant complex of raspberry fruit. The obtained results will form the base for further research aimed at investigating phytochemical compounds of fruit crops which have become an essential part of healthy human diet and developing nutraceutical products.


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