Analysis of the Essential Oil of the Aerial Parts of the Medicinal Plant Aristolochia indica Linn. (Aristolochiaceae) from South-India

The essential oil of the aerial parts of Aristolochia indica Linn. (Anstolochiaceae) from South-India was analyzed by gas chromatographic-spectroscopic (GC-FID and GC-MS) and olfactoric methods to identify those compounds responsible for the characteristic odor as well as partly for the folk medicinal use of this plant. Especially sesqui- and monoterpenes were found to be dominating constituents of this essential oil, such as: β-caryophyllene, α-humulene, ishwarone, caryophyllene oxide I, ishwarol, ishwarane and aristolochene as well as linalool and α-terpinolene.The odor impression of the sample is described and the possible biological activity of some single volatiles shortly discussed.

Download Full-text

Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities

Planta Medica ◽

10.1055/a-1628-2299 ◽

2021 ◽

Author(s):

Pedro Modesto Nascimento Menezes ◽

Emanuella Chiara Valença Pereira ◽

Kátia Simoni Bezerra Lima ◽

Bismarques Augusto Oliveira da Silva ◽

Mariana Coelho Brito ◽

...

Keyword(s):

Medicinal Plant ◽

Cannabis Sativa ◽

Phenol Red ◽

Traditional Use ◽

Aerial Parts ◽

Medicinal Use ◽

Pharmacological Test ◽

Experimental Protocols ◽

Brazilian Northeast ◽

Future Therapy

Abstract Cannabis sativa is a millenary medicinal plant. However, contrary to worldwide paradigm-shifting, countries like Brazil still prohibit C. sativa cultivation and its medicinal use, even though many populations use aerial parts and roots of this plant for healthcare. As such, the objective of this work was to identify substances in the samples of the C. sativa roots, tracing a correlation with antitussive and expectorant effects. Therefore, samples of C. sativa roots were donated by the Polícia Federal Brasileira, and its aqueous extract (AECsR) was prepared with subsequent lyophilization, to maintain the material stability. After that, the material was analyzed by LC-MS to observe its chemical profile. Four samples (AECsR-A, B, C, and D) were tested in animal models of citric acid-induced cough (0.4 M) and phenol red expectoration (500 mg/kg). Using LC-MS it was possible to identify 5 molecules in C. sativa roots: p-coumaroyltyramine, tetrahydrocannabinol-C4, feruoiltyramine, anhydrocanabisativine, and cannabisativine. In experimental protocols, male mice (Mus musculus) were treated with samples of AECsR at doses of 12.5, 25, or 50 mg/kg regardless of the pharmacological test. In these tests, all samples showed the potential to treat cough and promote fluid expectoration, differing only in the dose at which these effects were observed. Therefore, the data showed that the C. sativa roots of the Brazilian Northeast showed antitussive and expectorant effects, even with intense secondary metabolitesʼ variation, which alters its potency, but not its effect. This highlights the importance of this medicinal plant for future therapy and corroborates to traditional use.

Download Full-text

Chemical Composition of the Essential Oil of Croton bonplandianus from India

Natural Product Communications ◽

10.1177/1934578x1400900234 ◽

2014 ◽

Vol 9 (2) ◽

pp. 1934578X1400900

Author(s):

Rajesh K. Joshi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Gas Chromatography Mass Spectrometry ◽

Caryophyllene Oxide ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D ◽

Total Oil

The essential oil obtained from the aerial parts of Croton bonplandianus Baill. was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 37 compounds have been identified, representing 96.2% of the total oil. The main constituents were identified as β-caryophyllene (16.7%), germacrene D (14.7%), borneol (8.3%), Z-β-damascenone (6.(%), isobornyl acetate (6.2%), α-humulene (6.1%), germacrene A (5.2%) and caryophyllene oxide (4.5%). The oil was rich in sesquiterpene hydrocarbons (60.1%).

Download Full-text

Chemical investigation of the essential oil of Laggera crispata (Vahl) Hepper & Wood from India

Journal of the Serbian Chemical Society ◽

10.2298/jsc100801048v ◽

2011 ◽

Vol 76 (4) ◽

pp. 523-528 ◽

Cited By ~ 4

Author(s):

Ram Verma ◽

Rajendra Padalia ◽

Chandan Chanotiya ◽

Amit Chauhan ◽

Anju Yadav

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oil ◽

Chemical Investigation ◽

Gas Chromatography Mass Spectrometry ◽

Western Himalayas ◽

Caryophyllene Oxide ◽

Oil Composition ◽

Aerial Parts ◽

Total Oil

Hydrodistilled essential oil of the aerial parts of Laggera crispata (Vahl) Hepper & Wood, collected from the Kumaon region of the western Himalayas was analysed by gas chromatography and gas chromatography-Mass Spectrometry. Eighty constituents, accounting for 83.9 % of the total oil composition, were identified. The oil was mainly dominated by sesquiterpenoids (45.3 %) and benzenoid compounds (33.9 %). Among them, 2,5-dimethoxy-p-cymene (32.2 %), 10-epi-?-eudesmol (14.7 %), ?-caryophyllene (6.9 %), and caryophyllene oxide (5.4 %) were major components of the oil.

Download Full-text

Chemical Composition of the Essential Oil of Baccharoides lilacina from India

Natural Product Communications ◽

10.1177/1934578x1300800331 ◽

2013 ◽

Vol 8 (3) ◽

pp. 1934578X1300800

Author(s):

Rajesh K. Joshi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oil ◽

Essential Oil Composition ◽

Gas Chromatography Mass Spectrometry ◽

Caryophyllene Oxide ◽

Oil Composition ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Total Oil

The essential oil composition from the aerial parts of Baccharoides lilacina (Dalzell & A. Gibson) M. R. Almeida was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 41 compounds have been identified, representing 97.4% of the total oil. The main constituents were identified as β-caryophyllene (27.7%), epi-α-cadinol (25.1%), caryophyllene oxide (9.9%), α-muurolol (7.6%), α-cadinene (6.1%) and α-cadinol 4.5%). The oil was found to be rich in oxygenated sesquiterpenes (47.1%) and sesquiterpene hydrocarbons (46.2%).

Download Full-text

Chemical Composition and Antibacterial Activity of the Essential Oils of Blainvillea Rhomboidea (Asteraceae)

Natural Product Communications ◽

10.1177/1934578x0600100510 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100

Author(s):

Andreza Maria L. Pires ◽

Maria Rose Jane R. Albuquerque ◽

Edson P. Nunes ◽

Vânia M. M. Melo ◽

Edilberto R. Silveira ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Caryophyllene Oxide ◽

Bacterial Strains ◽

Aerial Parts ◽

Leaf Oil

The essential oils of Blainvellea rhomboidea (Asteraceae) were obtained by hydrodistillation and analyzed by GC/MS and GC/FID. Initially, the essential oil from the aerial parts was investigated. From the 18 identified components, 5-indanol (14.5%) followed by p-cymen-8-ol (10.1%), β-caryophyllene (9.6%), caryophyllene oxide (9.6%), limonene (8.6%), terpinolene (7.8%), and spathulenol (7.7%) were the major constituents. The oil was tested against seven bacterial strains and the results showed significant antimicrobial activity. As a consequence, the essential oils from leaves and from flowers were analyzed separately. The major constituents of the leaf oil were terpinolene (21.2%), β-caryophyllene (19.2%), spathulenol (9.1%), caryophyllene oxide (7.4%), and bicyclogermacrene (7.1%), while the oil of the flowers contained terpinolene (28.1%), 5-indanol (16.3%), p-cymen-8-ol (15.3%) and limonene (14.7%) as prevalent compounds. The oils were tested against the same bacterial strains and the flower oil was the more active. These results indicated that the components of the essential oil from flowers seem to be responsible for the activity.

Download Full-text

The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils

Zeitschrift für Naturforschung C ◽

10.1515/znc-2016-0038 ◽

2016 ◽

Vol 71 (11-12) ◽

pp. 403-408 ◽

Cited By ~ 2

Author(s):

Mohaddese Mahboubi ◽

Elaheh Mahdizadeh ◽

Rezvan Heidary Tabar

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Inhibition Zone ◽

Chemical Compositions ◽

Caryophyllene Oxide ◽

Aerial Parts ◽

Dilution Assay ◽

Main Components ◽

Broth Dilution

Abstract The purpose of our study was to compare the chemical compositions and antimicrobial and antioxidant activities of Pycnocycla spinosa and Pycnocycla flabellifolia essential oils. cis-Asarone (62.5%) and widdra-2,4(14)-diene (9%) were the main components of P. spinosa aerial part essential oil, while elemicin (60.1%) and caryophyllene oxide (9.8%) were the main components of P. spinosa seed essential oil. α-Phellandrene (25.5%), p-cymene (15.3%), and limonene (13.3%) were found in P. flabellifolia essential oil. The inhibition zone diameters for P. flabellifolia essential oil were significantly higher than for the two other essential oils from P. spinosa (p<0.05). In broth dilution assay (µL/mL), the sensitive microorganism to Pycnocycla sp. (P. spinosa, P. flabellifolia) was Aspergillus niger, followed by Candida albicans. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) system, P. spinosa aerial parts essential oil (IC50=548 µg/mL) had higher antioxidant activity than that of two other essential oils.

Download Full-text

Essential Oil Composition of the Aerial Parts of Bupleurum Candollii

Natural Product Communications ◽

10.1177/1934578x0800301125 ◽

2008 ◽

Vol 3 (11) ◽

pp. 1934578X0800301

Author(s):

Rajesh K. Joshi ◽

Chitra Pande

Keyword(s):

Essential Oil ◽

Methyl Ether ◽

Essential Oil Composition ◽

Caryophyllene Oxide ◽

Oil Composition ◽

Aerial Parts ◽

Total Oil

The essential oil composition of the aerial parts of Bupleurum candollii Wall. ex DC. was analyzed by GC and GC/MS. A total of 19 compounds have been identified, representing 91.8% of the total oil. The main constituents were identified as β-caryophyllene (34.1%), caryophyllene oxide (20.7%), dehydro-aromadendrene (7.6%) and thymol methyl ether (7.2%).

Download Full-text

ESTUDO ALELOPÁTICO APLICADO DE ASTER LANCEOLATUS, WILLD.

Visão Acadêmica ◽

10.5380/acd.v6i1.577 ◽

2005 ◽

Vol 6 (1) ◽

Author(s):

Joseane F. G. Dias ◽

Marilis Dallarmi Miguel

Keyword(s):

Essential Oil ◽

Lactuca Sativa ◽

Inhibitory Influence ◽

Caryophyllene Oxide ◽

Aerial Parts ◽

Applied Study ◽

Hexahydrofarnesyl Acetone ◽

Pure Compounds ◽

Stems And Leaves ◽

Asteraceae Family

O presente trabalho realizou estudo sobre Aster lanceolatus, Willd., pertencente a família Asteraceae, cujas partes aéreas foram divididas em duas porções. A primeira, composta de flores e a segunda, de caules e folhas. Da análise de CG nas frações hexano, verificou-se presença de β-sitosterol. Utilizando-se resultados obtidos com o estudo alelopático dos extratos etanólicos e frações, realizaram-se estudos fitoquímicos com o intuito de isolar compostos com provável atividade alelopática e testá-los com técnica desenvolvida denominada alelopatografia. Verificou-se a presença de espinasterol e canferol-rhamnosil-galactosídeo que ao serem submetidos a alelopatografia apresentaram influência alelopática inibitória da germinação e formação de folíolos. Da análise de CG/EM realizada com o óleo essencial, identificou-se treze constituintes: Mirtenol; α-Muroleno; 1,2-dihidro-1,1,6-trimetil Naftaleno; Bisaboleno; β-Lanona; Espatulenol; Oxido de Cariofileno; 3-Ciclohexeno-1-carboxaldeído; 3,4, Cedren-13-ol, 8; Neocloveno-(I) dihidro; Azuleno 1-4-dimetil-7-(1-metila); 2H-Benzociclohepteno-2-ona, 1,4a,5,6,7,8,9,9a-octahidro 4a metil trans e 1, hexahidrofarnesil acetona. A água aromática quando submetida a ensaio alelopático apresentou atividade alelopática inibitória da germinação e do crescimento de hipocótilo de Lactuca sativa, provavelmente devido aos constituintes do óleo essencial. De acordo com análise comparativa dos ensaios alelopáticos, pode-se referir que a influência alelopática é mais intensa ao utilizar-se fração inteira em comparação aos compostos isolados, e que a alelopatografia pode representar um método mais rápido e de fácil visualização de influência alelopática de constituintes impuros. Até este momento, não havia relatos da presença dos constituintes químicos relatados neste trabalho em Aster lanceolatus. Palavras chave: alelopatografia, alelopatia, fitoquímica, atividade antifúngica e antibacteriana. Allelophatic Applied Study from Aster lanceolatus, Willd Abstract The present work accomplished study about Aster lanceolatus, Willd., belonging the Asteraceae family, whose aerial parts were shared in two portions. The first one was composed of flowers and the second one of stems and leaves. Trough the analysis of CG accomplished in the hexan fractions, a presence of β-sitosterol has been verified. Using the results obtained with the allelophatic study of the ethanolics extracts and fractions, it has taken place the phytochemistries studies with the intention of to isolate composed with probable allelophatic activity and to test with a developed technique called allelophatography. It has been verified a presence of spinasterol and kaempferol-rhamnosyl-galactoside, which presented inhibitory influence of the germination and leaves formation when the allelopatography has been submitted. Through the analysis of CG/MS accomplished with the essential oil, thirteen compounds has been verified: myrtenol; α-muurolene; naphthalene, 1,2 dihydro-1,1,6-trimethyl; bisabolene; β-lanona; spathulenol; caryophyllene oxide; 3-cyclohexen-1-carboxaldehyde, 3, 4; cedren-13-ol, 8; neoclovene-(I), dihydro; azulene, 1,4-dimethyl-7-(1-methyle); 2H-benzocyclohepten-2-one, 1, 4a, 5, 6, 7, 8, 9, 9a-octahydro 4a, methyl, trans; 1, hexahydrofarnesyl acetone. The aromatic water when submitted in the allelophatic study showed inhibitory allelophatic activity from the germination and the growth of hypocotyl of Lactuca sativa, probably because the essential oil fractions. In agreement with comparative analysis of the allelophatics rehearsals, it can refer the allelophatic influence is more intense when using complete fraction in comparison with the isolated compounds, and the allelophatography can represent a faster and easier method of visualization from the allelophatic influence of non-pure compounds. From now there have been no reports about the presence of chemistry compounds reported in this work in Aster lanceolatus. Key words: allelophatography, allelophaty, phytochemistry, antifungal and antibacterial activity.

Download Full-text

Essential Oil Constituents ofTanacetum cilicicum: Antimicrobial and Phytotoxic Activities

Journal of Food Quality ◽

10.1155/2017/6214896 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Zeynep Ulukanli ◽

Salih Demirci ◽

Murat Yilmaztekin

Keyword(s):

Essential Oil ◽

Mediterranean Region ◽

Industrial Applications ◽

Caryophyllene Oxide ◽

East Mediterranean ◽

First Report ◽

Oxygenated Monoterpenes ◽

Aerial Parts ◽

Antimicrobial Assay ◽

East Mediterranean Region

Aerial parts ofTanacetum cilicicumwere hydrodistillated for 3 h using Clevenger. Essential oil (EO) yield was 0.4% (v/w). According to the GC/MS analyses, EO ofT. cilicicumconsisted of monoterpenes [α-pinene (2.95 ± 0.19%), sabinene (2.32 ± 0.11%), and limonene (3.17 ± 0.25)], oxygenated monoterpenes [eucalyptol (5.08 ± 0.32%), camphor (3.53 ± 0.27%), linalool (7.01 ± 0.32%),α-terpineol (3.13 ± 0.23%), and borneol (4.21 ± 0.17%)], and sesquiterpenes [sesquisabinene hydrate (6.88 ± 0.41%), nerolidol (4.90 ± 0.33%),α-muurolol (4.57% ± 0.35), spathulanol (2.98 ± 0.12%), juniper camphor (2.68 ± 0.19%), (-)-caryophyllene oxide (2.64 ± 0.19%), 8-hydroxylinalool (2.62 ± 0.15%), andΔ-cadinene (2.48 ± 0.16%)]. In the antimicrobial assay, MIC/MBC values of the EO were the most significant onB. subtilis(0.39/0.78 µL/mL) andB. cereus(0.78/1.56 µL/mL).The most prominent phytotoxic activities of the EO were observed onL. sativa,L. sativum, andP. oleracea. The results of the present study indicated that EO ofT. cilicicumincludes various medicinally and industrially crucial phytoconstituents that could be in use for industrial applications. The finding of this study is the first report on this species from the East Mediterranean region.

Download Full-text