scholarly journals BIOHYDROGEN PRODUCTION FROM WASTES OF PLANT AND ANIMAL ORIGIN VIA DARK FERMENTATION

2019 ◽  
Vol 27 (2) ◽  
pp. 101-113 ◽  
Author(s):  
Weronika Cieciura-Włoch ◽  
Sebastian Borowski
Keyword(s):  
Hydrogen Production ◽  
Sugar Beet ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Animal Origin ◽  
Hydrogen Yield ◽  
Vegetable Waste ◽  
Fruit And Vegetable ◽  
Kitchen Waste ◽  
High Hydrogen

This study investigated the batch experiments on biohydrogen production from wastes of plant and animal origin. Several substrates including sugar beet pulp (SBP), sugar beet leaves (SBL), sugar beet stillage (SBS), rye stillage (RS), maize silage (MS), fruit and vegetable waste (FVW), kitchen waste (KW) and slaughterhouse waste (SHW) including intestinal wastes, meat tissue, post flotation sludge were tested for their suitability for hydrogen production. Generally, the substrates of plant origin were found to be appropriate for dark fermentation, and the highest hydrogen yield of 280 dm3 H2/kg VS was obtained from fruit and vegetable waste. Contrary to these findings, slaughterhouse waste as well as kitchen waste turned out to be unsuitable for hydrogen production although their methane potential was high. It was also concluded that the combined thermal pretreatment with substrate acidification was needed to achieve high hydrogen yields from wastes.

scholarly journals Biohydrogen production by fermentive bacterium Clostridium sp. Tr2 using batch fermenter system controlled pH under dark fermentation

2018 ◽  
Vol 9 (1) ◽  
pp. 4-10
Author(s):  
Thi Thu Huyen Nguyen ◽  
Thi Yen Dang ◽  
Thuy Hien Lai
Keyword(s):  
Hydrogen Production ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
Dark Fermentation ◽  
Gas Production ◽  
Biohydrogen Production ◽  
Great Promise ◽  
Hydrogen Yield ◽  
Promising Alternative ◽  
Fermentative Bacteria

Limitation of fuels reserves and contribution of fossil fuels to the greenhouse effect leads to develop anew, clean and sustainable energy. Among the various options, biohydrogen appears as a promising alternative energy source. The fermentative hydrogen production process holds a great promise for commercial processes. Hydrogen production by fermentative bacteria is a very complex and greatly influenced by pH. This paper presents biohydrogen production by bacterial strain Clostridium sp. Tr2. Operational pH strongly affected its hyrogen production. Its gas production rate as well as obtained gas product were roughly increase twice under controlled pH at 6 than non-controlled condition. Dark fermentation for hydrogen production of strain Tr2 was performed under bottle as well as automatic fermenter scale under optimal nutritional and environmental conditions at 30°C, initial pH at 6.5, then pH was controlled at 6 for bioreactor scale (BioFlo 110). Bioreactor scale was much better for hydrogen production of strain Tr2. Clostridium sp. Tr2 produced 0.74 L hydro (L medium)-1 occupying 72.6 % of total gas under bottle scale while it produced 2.94 L hydro (L medium)-1 occupying 95.82 % of total gas under fermenter scale. Its maximum obtained hydrogen yield of Clostridium sp. Tr2 under bioreactor scale Bioflo 110 in optimal medium with controlled pH 6 was 2.31 mol hydro (mol glucose)-1. Dự trữ nhiên liệu có giới hạn và việc sử dụng nhiên liêu hoá thạch góp phần không nhỏ gây hiệu ứng nhà kính dẫn đến cần phải phát triển năng lượng mới, sạch và bền vững. Trong số các giải pháp, hydro sinh học xuất hiện như một nguồn năng lượng thay thế đầy hứa hẹn. Quá trình lên men sản xuất hydro có tiềm năng lớn để áp dụng trong sản xuất thương mại. Tuy nhiên qúa trình này rất phức tạp và chịu ảnh hưởng lớn bởi pH. Nghiên cứu này trình bày sản xuất hydro sinh học do chủng vi khuẩn Clostridium sp. Tr2. Quá trình sản xuất hydro của chủng này bị ảnh hưởng mạnh mẽ bởi pH thay đổi trong quá trình lên men. Tốc độ tạo khí cũng như lượng khí thu được của chủng này tăng gần gấp đôi trong môi trường có duy trì pH ở pH 6 so với môi trường không kiểm soát pH. Quá trình lên men tối sản xuất hydro của chủng Tr2 được thực hiện ở quy mô bình thí nghiệm cũng như bình lên men tự động trong điều kiện môi trường tối ưu ở 30°C, pH ban đầu 6.5, ở qui mô bình lên men tự động (BioFlo 110), pH môi trường sau đó được duy trì ổn định ở pH 6. Lên men sản xuất hdyro của chủng Tr2 trong bình lên men tự động tốt hơn rất nhiều so với lên men trong bình thí nghiệm. Clostridium sp. Tr2 chỉ tạo ra được 0,74 L hydro (L medium)-1 chiếm 72,6 % tổng thể tích khí thu được ở điều kiện lên men bình thí nghiệm trong khi chủng này sản xuất được 2,94 L hydro (L medium)-1 chiếm 95,82 % tổng thể tích khí ở điều kiện lên men tự động. Sản lượng hydro thu được lớn nhất của chủng này trong bình lên men tự động BioFlo 110 trong trong môi trường tối ưu có kiểm soát pH tại pH 6 là 2,31 mol hydro (mol glucose)-1.

scholarly journals Investigating Dark Fermentation as a Sustainable Organic Waste Management Technology for Producing Biohydrogen From Fruit and Vegetable Waste

2021 ◽  
Author(s):  
Primrose Magama ◽  
Idan Chiyanzu ◽  
Jean Mulopo
Keyword(s):  
Waste Management ◽  
Waste Treatment ◽  
Dark Fermentation ◽  
Valeric Acid ◽  
Hydrogen Yield ◽  
Vegetable Waste ◽  
Fruit And Vegetable ◽  
Promising Alternative ◽  
Management Technology ◽  
Pre Treatment

Abstract Fruit and vegetable waste (FVW) is made up of biodegradable organic compounds whose disposal in landfills leads to water and environmental pollution and the emission of lethal greenhouse gases such as methane. Given the high organic content of FVW and its abundant and constant supply, FVW can serve as valuable feedstock for energy production and other value added bio-commodities. Whilst anaerobic digestion is a mature and proven waste management technology, there is need to investigate and develop other environmentally friendly waste treatment technologies. Dark fermentation is a promising alternative waste treatment technology that can be utilized to generate biohydrogen, however, more R&D is still required to improve process efficiency and enhance hydrogen yields. This study investigated the production of biohydrogen via dark fermentation using fruit and vegetable waste as a substrate. To enhance hydrogen production the study applied seed inoculum pre-treatment including heat, alkali, acid, and heat-alkali. The results show that FVW can be used as a feedstock for biohydrogen production and that inoculum pre-treatment enhances hydrogen yields. Acid pre-treatment resulted in the highest hydrogen yield (142, 74 Nml/g VS) and hydrogen content (54%) while heat pre-treatment generated the lowest hydrogen yield (0, 90 Nml/g VS). The hydrogen yields of the pre-treatments tested were substantially different (p<0.001). From highest to lowest, the order of pre-treatment efficacy in terms of hydrogen yield enhancement was acid>heat-alkali>alkali>heat. Under all pre-treatments and controls, the main volatile fatty acid (VFA) formed was valeric acid. The maximum valeric acid fraction observed was 86.1% under acid inoculum pre-treatment.

scholarly journals Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. M. Silva ◽  
A. A. Abreu ◽  
A. F. Salvador ◽  
M. M. Alves ◽  
I. C. Neves ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Anaerobic Bacteria ◽  
Dark Fermentation ◽  
Inorganic Materials ◽  
Biohydrogen Production ◽  
High Rate ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Fermentation Products ◽  
Zeolite Type

AbstractThermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.

Bio-electrochemical production of hydrogen and electricity from organic waste

2021 ◽  
Author(s):  
Giorgia De Gioannis ◽  
Alessandro Dell'Era ◽  
Aldo Muntoni ◽  
Mauro Pasquali ◽  
Alessandra Polettini ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Electron Flow ◽  
Dark Fermentation ◽  
Electrochemical Process ◽  
Integrated System ◽  
Galvanic Cell ◽  
Hydrogen Yield ◽  
Cell Coupling ◽  
Production Of Hydrogen ◽  
Acid Metabolites

Abstract This study investigated the performance of a novel integrated bio-electrochemical system for synergistic hydrogen production from a process combining a dark fermentation reactor and a galvanic cell. The operating principle of the system is based on the electrochemical conversion of protons released upon dissociation of the acid metabolites of the biological process and is mediated by the electron flow from the galvanic cell, coupling biochemical and electrochemical hydrogen production. Accordingly, the galvanic compartment also generates electricity. Four different experimental setups were designed to provide a preliminary assessment of the integrated bio-electrochemical process and identify the optimal configuration for further tests. Subsequently, dark fermentation of cheese whey was implemented both in a stand-alone biochemical reactor and in the integrated bio-electrochemical process. The integrated system achieved a hydrogen yield in the range 75.5 – 78.8 N LH2/kg TOC, showing a 3 times improvement over the biochemical process.

Enhancing Hydrogen Production Performance by Developing Thermotolerant Fermentative Mixed Consortium via High Temperature Stress Acclimation

2020 ◽  
Vol 14 (3) ◽  
pp. 340-348
Author(s):  
Jinyu Du ◽  
Qinglin Wu ◽  
Weixing Cao ◽  
Jian Wang ◽  
Nirmal Joshee ◽  
...  
Keyword(s):  
High Temperature ◽  
Hydrogen Production ◽  
Temperature Stress ◽  
High Temperature Stress ◽  
Production Performance ◽  
Biohydrogen Production ◽  
High Hydrogen ◽  
Metabolic Characteristics ◽  
Stress Acclimation ◽  
Tropical Areas

A thermotolerant fermentative mixed consortium (TFMC) has been screened by directional high temperature stress acclimation for biohydrogen production. The kinetics and metabolic characteristics of TFMC for hydrogen production at high temperature has also been studied. the TFMC can achieve stable hydrogen production under mesophilic conditions at 38 °C with the maximum potential hydrogen production of 665.09 mL, which is decreases by 9.27% as compared to the original mixed consortium at 30 °C. The TFMC also has characteristic of short lag time of 27 h which is 22 h lower than initial group and high hydrogen production rate of 9.48 mL/h which improved 12.9%. The newly obtained TFMC highlights a great potential for biohydrogen production in tropical areas.

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