scholarly journals Prevalence of virulence genes and their association with biofilm formation in VRE faecium isolates from Ahvaz, Iran

2018 ◽  
Vol 12 (11) ◽  
pp. 970-977
Author(s):  
Saber Soltani ◽  
Maniya Arshadi ◽  
Muhammad Ibrahim Getso ◽  
Farzaneh Aminharati ◽  
Mahmood Mahmoudi ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Positive Association ◽  
Microtiter Plate ◽  
Clinical Specimens ◽  
Microbiological Methods ◽  
Antibiotic Susceptibility Patterns ◽  
Pcr Method ◽  
Susceptibility Patterns

Introduction: Vancomycin-resistant Enterococcus faecium (VREfm) is a common cause of nosocomial infections. Biofilm formation is an important factor in recurrence of infections, facilitating transfer of genetic elements, leading to treatment failures. The aim of this study was to investigate the virulence genes in biofilm producing isolates and to determine possible association between biofilm formation and the presence of these genes; also to determine association between antibiotic susceptibility patterns of VREfm isolates and their biofilm formation ability. Methodology: A total of 57 isolates of VREfm were recovered from different sources of hospitals under Ahvaz University, Iran. The isolates were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility patterns of the isolates were determined by disk-diffusion and E-test. The biofilm formation ability of the isolates was investigated by Modified Congo red agar and microtiter plate techniques. The presence of virulence genes was examined using Multiplex-PCR method. Results: Out of 57 VREfm isolates, 63.15% of isolates were biofilm producers. The frequency of biofilm producing isolates from clinical specimens, colonized patients and environmental sources were 78.26%, 60%, and 42.85%, respectively. The prevalence of acm, esp and hyl genes among biofilm producing isolates was 86.10%, 55.56% and 52.77%, respectively. There was statistically significant association between esp gene and biofilm formation among isolates from the clinical specimens. Conclusion: Clinical isolates producing biofilms showed a positive association with the presence of the esp. Our study further suggests that the link between virulence genes and biofilms is affected by the environmental context.

scholarly journals Correlation of biofilm production with antibiotic susceptibility pattern of Pseudomonas aeruginosa from various clinical specimens

2022 ◽  
Vol 13 (1) ◽  
pp. 88-92
Author(s):  
M Swapna ◽  
G Sumathi ◽  
M Anitha
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Antibiotic Sensitivity ◽  
Microtiter Plate ◽  
Resistance Pattern ◽  
Sensitivity Testing ◽  
Clinical Specimens ◽  
Biofilm Production ◽  
Microbiological Methods

Background: Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogens that cause a life-threatening infection. One of the important characteristics of P. aeruginosa is biofilm formation which leads to antibiotic resistance. Aims and Objectives: The aim of the study was to study the antibiotic resistance pattern of P. aeruginosa isolates and correlation with their biofilm-production. Materials and Methods: A total of 87 P. aeruginosa isolates from different clinical specimens were processed and confirmed by conventional microbiological methods as per standard methodology. Antibiotic sensitivity testing was done for all isolates. Biofilm producing isolates were identified by the microtiter plate method (MTPM). Results: Of 87 P. aeruginosa isolates, majority were from pus 33 (38%), followed by urine 26 (30%), sputum 19 (22%), body fluids 7 (8%), and blood 2 (2%). Biofilm producing isolates showed more resistance in comparison to non-biofilm producers. The observed difference between biofilm formation for multidrug resistant and susceptible isolates was found to be statistically significant. Conclusion: MTPM method was an effective test for detection of biofilm formation and was also able to verify biofilm production by P. aeruginosa. This indicated a higher propensity among the clinical isolates of P. aeruginosa to form biofilm and revealed a positive correlation between biofilm formation and antibiotic resistance. This indicates the need for testing of even susceptible isolates for virulence factors such as biofilm production.

scholarly journals A study of bacterial pathogens and antibiotic susceptibility patterns in chronic suppurative otitis media

2017 ◽  
Vol 132 (1) ◽  
pp. 41-45 ◽  
Author(s):  
M R Mofatteh ◽  
F Shahabian Moghaddam ◽  
M Yousefi ◽  
M H Namaei
Keyword(s):  
Otitis Media ◽  
Antibiotic Susceptibility ◽  
Chronic Suppurative Otitis Media ◽  
Bacterial Isolates ◽  
Suppurative Otitis Media ◽  
Microbiological Methods ◽  
Significant Difference ◽  
Antibiotic Susceptibility Patterns ◽  
Periodic Evaluation ◽  
Susceptibility Patterns

AbstractObjective:To assess the frequency of bacterial agents in chronic suppurative otitis media and the antibiotic susceptibility patterns of isolates among patients.Methods:A total of 185 patients clinically diagnosed with chronic suppurative otitis media were interviewed and middle-ear effusion samples were collected using sterile swabs. All bacterial isolates were identified by conventional microbiological methods. Antibiotic susceptibility patterns of the isolates were determined by Kirby–Bauer disc diffusion.Results:Staphylococci spp. (64.9 per cent) were the most prevalent bacteria isolated, followed by Klebsiella spp. (12.9 per cent) and Pseudomonas aeruginosa (10.3 per cent). The most effective antibiotic for treatment of bacterial chronic suppurative otitis media was ciprofloxacin. Statistical analysis showed no significant difference in bacterial infestations among chronic suppurative otitis media patients and the antimicrobial susceptibility patterns of the bacterial isolates based on gender and age (p > 0.05).Conclusion:Our findings highlight the importance of a continuous and periodic evaluation of the bacteriological profile and antibiotic susceptibility patterns in chronic suppurative otitis media patients for efficacious treatment of the infection.

scholarly journals Antibiotic Susceptibility Patterns, Biofilm Formation and esp Gene among Clinical Enterococci: Is There Any Association?

2019 ◽  
Vol 16 (18) ◽  
pp. 3439 ◽  
Author(s):  
Poh Leng Weng ◽  
Ramliza Ramli ◽  
Rukman Awang Hamat
Keyword(s):  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Cross Sectional Study ◽  
Control Measures ◽  
Effective Control ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns

Enterococci are commonly found in humans, animals and environments. Their highly adaptive mechanisms are related to several virulent determinants and their ability to resist antibiotics. Data on the relationship between the esp gene, biofilm formation and antibiotic susceptibility profiles may differ between countries. This cross-sectional study was conducted to determine the proportion of esp gene and biofilm formation among Enterococcus faecalis and Enterococcus faecium clinical isolates. We also investigated the possible association between the esp gene with antibiotic susceptibility patterns and biofilm formation. The isolates were collected from clinical samples and identified using biochemical tests and 16SRNA. Antibiotic susceptibility patterns and a biofilm assay were conducted according to the established guidelines. Molecular detection by PCR was used to identify the esp gene using established primers. In total, 52 and 28 of E. faecalis and E. faecium were identified, respectively. E. faecium exhibited higher resistance rates compared to E. faecalis as follows: piperacillin/tazobactam (100% versus 1.9%), ampicillin (92.8% versus 1.9%), high-level gentamicin resistance (HLGR) (89.3% versus 25.0%) and penicillin (82.1% versus 7.7%). E. faecium produced more biofilms than E. faecalis (59.3% versus 49.0%). E. faecium acquired the esp gene more frequently than E. faecalis (78.6% versus 46.2%). Interestingly, the associations between ampicillin and tazobactam/piperacillin resistance with the esp gene were statistically significant (X2 = 4.581, p = 0.027; and X2 = 6.276, p = 0.012, respectively). Our results demonstrate that E. faecium exhibits high rates of antimicrobial resistance, esp gene acquisition and biofilm formation. These peculiar traits of E. faecium may have implications for the management of enterococcal infections in hospitals. Thus, concerted efforts by all parties in establishing appropriate treatment and effective control measures are warranted in future.

Antibiotic susceptibility patterns and clones of Pseudomonas aeruginosa in Swedish ICUs

2008 ◽  
Vol 40 (6-7) ◽  
pp. 487-494 ◽  
Author(s):  
Marcus Erlandsson ◽  
Hans Gill ◽  
David Nordlinder ◽  
Christian G. Giske ◽  
Daniel Jonas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns
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