scholarly journals Functional Pattern of Increasing Concentrations of Brain-Derived Neurotrophic Factor in Spiral Ganglion: Implications for Research on Cochlear Implants

2017 ◽  
Vol 5 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Emina Ramku ◽  
Refik Ramku ◽  
Dugagjin Spanca ◽  
Valbona Zhjeqi
Keyword(s):  
Neurotrophic Factor ◽  
Spiral Ganglion ◽  
Brain Derived Neurotrophic Factor ◽  
Future Research ◽  
Nmri Mice ◽  
Hearing Research ◽  
Neurite Density ◽  
Show Evidence ◽  
Spiral Ganglia ◽  
Research Institute

BACKGROUND: As previously various studies have suggested application of brain-derived neurotrophic factor (BDNF) may be considered as a promising future therapy for hearing deficits, in particular for the improvement of cochlear neurone loss during cochlear implantation.AIM: The present study's aim was to establish the upper threshold of the concentration of BDNF in Naval Medical Research Institute (NMRI) mice spiral ganglion outgrowth.METHODS: Spiral ganglion explants were prepared from post-natal day 4 (p4) (NMRI) mice of both sexes under the approval and guidelines of the regional council of Hearing Research Institute Tubingen.RESULTS: Spiral ganglion explants were cultured at postnatal days 4 in the presence of different concentrations of BDNF as described under methods. We chose an age of postnatal day (P4) and concentrations of BDNF 0; 6; 12.5; 25 and 50 ƞg/ml. Averaged neurite outgrowth is measured in 4 different cultures that were treated with different concentrations. Results show that with increasing concentrations of BDNF, the neurite density increases.CONCLUSION: The present finding show evidence that BDNF has a clear incremental effect on the number of neurites of spiral ganglia in the prehearing organ, but less on the neurite length. The upper threshold of exogenous BNDF concentration on spiral ganglion explant is 25 ƞg/ml. This means that concentration beyond this level has no further incremental impact. Therefore our suggestion for hydrogel concentration in NMRA mice in future research should be 25 ƞg/ml.

scholarly journals Consecutive Treatment with Brain-Derived Neurotrophic Factor and Electrical Stimulation Has a Protective Effect on Primary Auditory Neurons

2020 ◽  
Vol 10 (8) ◽  
pp. 559 ◽  
Author(s):  
Verena Scheper ◽  
Ira Seidel-Effenberg ◽  
Thomas Lenarz ◽  
Timo Stöver ◽  
Gerrit Paasche
Keyword(s):  
Electrical Stimulation ◽  
Neurotrophic Factor ◽  
Combined Treatment ◽  
Spiral Ganglion ◽  
Brain Derived Neurotrophic Factor ◽  
Auditory Brainstem ◽  
Contralateral Side ◽  
Auditory Brainstem Responses ◽  
Drug Delivery Device ◽  
Ganglion Neurons

Degeneration of neurons, such as the inner ear spiral ganglion neurons (SGN), may be decelerated or even stopped by neurotrophic factor treatment, such as brain-derived neurotrophic factor (BDNF), as well as electrical stimulation (ES). In a clinical setting, drug treatment of the SGN could start directly during implantation of a cochlear implant, whereas electrical stimulation begins days to weeks later. The present study was conducted to determine the effects of consecutive BDNF and ES treatments on SGN density and electrical responsiveness. An electrode drug delivery device was implanted in guinea pigs 3 weeks after deafening and five experimental groups were established: two groups received intracochlear infusion of artificial perilymph (AP) or BDNF; two groups were treated with AP respectively BDNF in addition to ES (AP + ES, BDNF + ES); and one group received BDNF from the day of implantation until day 34 followed by ES (BDNF ⇨ ES). Electrically evoked auditory brainstem responses were recorded. After one month of treatment, the tissue was harvested and the SGN density was assessed. The results show that consecutive treatment with BDNF and ES was as successful as the simultaneous combined treatment in terms of enhanced SGN density compared to the untreated contralateral side but not in regard to the numbers of protected cells.

The biological effects of cell-delivered brain-derived neurotrophic factor on cultured spiral ganglion cells

Neuroreport ◽  
2007 ◽  
Vol 18 (16) ◽  
pp. 1683-1686 ◽  
Author(s):  
Athanasia Warnecke ◽  
Kirsten Wissel ◽  
Andrea Hoffmann ◽  
Nicola Hofmann ◽  
Nurdanat Berkingali ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Ganglion Cells ◽  
Biological Effects ◽  
Spiral Ganglion ◽  
Brain Derived Neurotrophic Factor

scholarly journals Relationship between exclusive breastfeeding and brain-derived neurotrophic factor in children

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248023
Author(s):  
Carlos Berlanga-Macías ◽  
Mairena Sánchez-López ◽  
Montserrat Solera-Martínez ◽  
Ana Díez-Fernández ◽  
Inmaculada Ballesteros-Yáñez ◽  
...  
Keyword(s):  
Exclusive Breastfeeding ◽  
Sexual Maturation ◽  
Neurotrophic Factor ◽  
Developmental Stages ◽  
Serum Levels ◽  
Brain Derived Neurotrophic Factor ◽  
Future Research ◽  
Significant Positive Association ◽  
Cross Sectional ◽  
The Relationship

Objective A positive relationship between breastfeeding and brain-derived neurotrophic factor (BDNF) in infants has been suggested due to the presence of BDNF in human milk. This study aimed to determine the relationship between exclusive breastfeeding and BDNF serum levels in Spanish schoolchildren. Methods A cross-sectional analysis including 202 schoolchildren, aged eight to 11 years, from Cuenca, Spain, was conducted. Information on sociodemographic and anthropometric variables, sexual maturation, birth weight and exclusive breastfeeding (‘no exclusive breastfeeding’, and exclusive breastfeeding for ≤6 and >6 months), and BDNF serum levels using an ELISA method were obtained. Covariance analyses (ANCOVA) were conducted to examine the relationship between serological BDNF and exclusive breastfeeding after controlling for potential confounders. Results ANCOVA models showed no significant differences in BDNF levels between children who were exclusively breastfed for more than six months versus those who were not (p > 0.05). No significant differences were observed by age group (eight to nine years versus 10 to 11 years; p > 0.05). Additionally, no clear negative trend in BDNF serum levels according to sexual maturation categories was found (p > 0.05). Conclusion These findings suggest that exclusive breastfeeding does not have a significant positive association on BDNF from eight to 11 years, since children who were exclusively breastfed did not have significantly higher BDNF levels than those who were not exclusively breastfed. Likewise, BDNF levels were not found to be negatively affected by hormonal development. Future research should examine the influence of exclusive breastfeeding on BDNF over the different developmental stages.

The Peripheral Processes of Spiral Ganglion Cells After Intracochlear Application of Brain-Derived Neurotrophic Factor in Deafened Guinea Pigs

2013 ◽  
Vol 34 (3) ◽  
pp. 570-578 ◽  
Author(s):  
Laurien Waaijer ◽  
Sjaak F. L. Klis ◽  
Dyan Ramekers ◽  
Martinus H. W. Van Deurzen ◽  
Ferry G. J. Hendriksen ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Guinea Pigs ◽  
Ganglion Cells ◽  
Spiral Ganglion ◽  
Brain Derived Neurotrophic Factor

scholarly journals Brain-Derived Neurotrophic Factor in Central Nervous System Myelination: A New Mechanism to Promote Myelin Plasticity and Repair

2018 ◽  
Vol 19 (12) ◽  
pp. 4131 ◽  
Author(s):  
Jessica Fletcher ◽  
Simon Murray ◽  
Junhua Xiao
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neural Development ◽  
Neurotrophic Factor ◽  
Molecular Mechanisms ◽  
Demyelinating Disease ◽  
Cell Types ◽  
Brain Derived Neurotrophic Factor ◽  
Future Research ◽  
Health And Disease

Brain-derived neurotrophic factor (BDNF) plays vitally important roles in neural development and plasticity in both health and disease. Recent studies using mutant mice to selectively manipulate BDNF signalling in desired cell types, in combination with animal models of demyelinating disease, have demonstrated that BDNF not only potentiates normal central nervous system myelination in development but enhances recovery after myelin injury. However, the precise mechanisms by which BDNF enhances myelination in development and repair are unclear. Here, we review some of the recent progress made in understanding the influence BDNF exerts upon the myelinating process during development and after injury, and discuss the cellular and molecular mechanisms underlying its effects. In doing so, we raise new questions for future research.

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