scholarly journals Welcome to the Czech Republic again! Rare northern mosses Calliergon megalophyllum and Drepanocladus sordidus (Amblystegiaceae) in South Bohemia in light of their European distribution and habitat preferences

PhytoKeys ◽  
2020 ◽  
Vol 154 ◽  
pp. 111-136
Author(s):  
Łukasz Krajewski ◽  
Lubomír Adamec ◽  
Marta Saługa ◽  
Halina Bednarek-Ochyra ◽  
Vítězslav Plášek
Keyword(s):  
Czech Republic ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Habitat Preferences ◽  
Internal Transcribed Spacers ◽  
Aquatic Moss ◽  
Moss Species ◽  
The Czech Republic ◽  
Morphological Studies ◽  
South Bohemia

Two aquatic moss species, Calliergon megalophyllum and Drepanocladus sordidus (Amblystegiaceae, Bryophyta), which had been considered extinct in the Czech Republic, were found in the Třeboň Basin, South Bohemia, in 2016–2017. They co-occurred in extensive reed- and sedge-dominated fen pools with humic water on the shore of an old fishpond and the former species was also discovered in a small humic pool in an old shallow sand-pit. The new Czech sites of these rare boreal species represent one of the southernmost known outposts within their entire European range. Previously, the two species were only known from single records in the Czech Republic from the late 19th and early 20th centuries. To confirm our morphological observations, we used phylogenetic analyses of DNA sequence variation in four chloroplast loci (atpB-rbcL, trnL-trnF, rpl16, trnG) and one nuclear region, the internal transcribed spacers of ribosomal DNA (ITS). We found (1) monophyly of all Calliergon megalophyllum specimens tested; (2) based on chloroplast DNA sequences, monophyly among all Drepanocladus sordidus specimens and representatives of Pseudocalliergon turgescens and P. lycopodioides moss species; (3) based on nuclear ITS sequences, monophyly of all original D. sordidus specimens. These results corroborate morphological studies and thus confirm the existence of natural sites for the studied moss species in the Třeboň Basin, South Bohemia, Czech Republic.

scholarly journals Pyrenochaetopsis kuksensis (Pyrenochaetopsidaceae), a new species associated with an ornamental boxwood in the Czech Republic

Phytotaxa ◽  
2021 ◽  
Vol 498 (3) ◽  
pp. 177-185
Author(s):  
MILAN ŠPETÍK ◽  
AKILA BERRAF-TEBBAL ◽  
ROBERT POKLUDA ◽  
ALEŠ EICHMEIER
Keyword(s):  
Czech Republic ◽  
New Species ◽  
Phylogenetic Relationships ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
New Taxon ◽  
The Czech Republic ◽  
Buxus Sempervirens ◽  
Fungal Microbiome ◽  
A New Species

During the investigation of fungal microbiome associated with boxwood in the Czech Republic, samples from Buxus sempervirens L. (Buxaceae) plants were collected and used for isolation. Two fungal strains were proposed as a new species Pyrenochaetopsis kuksensis based on morphology as well as phylogenetic analyses of ITS, LSU, rpb2, and tub2 sequence data. Detailed descriptions and phylogenetic relationships of the new taxon are provided.

scholarly journals Phylogenetics of Taxus Using the Internal Transcribed Spacers of Nuclear Ribosomal DNA and Plastid trnL-F Regions

Horticulturae ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 19
Author(s):  
Patricia Coughlan ◽  
James C. Carolan ◽  
Ingrid L. I. Hook ◽  
Lisa Kilmartin ◽  
Trevor R. Hodkinson
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Intergenic Spacer ◽  
Its Region ◽  
Single Species ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Trnl Intron ◽  
Group Status ◽  
Haplotype Networks

Taxus is a genus of trees and shrubs with high value in horticulture and medicine as a source of the anticancer drug paclitaxel. The taxonomy of the group is complex due to the lack of diagnostic morphological characters and the high degree of similarity among species. Taxus has a wide global geographic distribution and some taxonomists recognize only a single species with geographically defined subgroups, whereas others have described several species. To address these differences in taxonomic circumscription, phylogenetic analyses were conducted on DNA sequences using Maximum Likelihood, Bayesian Inference and TCS haplotype networks on single and combined gene regions obtained for the nuclear ribosomal ITS region and the plastid trnL intron and trnL-F intergenic spacer. Evidence is presented for the sister group status of Pseudotaxus to Taxus and the inclusion of Amentotaxus, Austrotaxus, Cephalotaxus and Torreya within Taxaceae. Results are consistent with the taxonomic recognition of nine species: T. baccata, T. brevifolia, T. canadensis, T. cuspidata, T. floridana, T. fuana, T. globosa, T. sumatrana and T. wallichiana, but evidence is found for less species distinction and considerable reticulation within the T. baccata, T. canadensis and T. cuspidata group. We compare the results to known taxonomy, biogeography, present new leaf anatomical data and discuss the origins of the hybrids T. ×media and T. ×hunnewelliana.

scholarly journals First detection and characterisation of porcine hemagglutinating encephalomyelitis virus in the Czech Republic

2019 ◽  
Vol 64 (No. 02) ◽  
pp. 60-66
Author(s):  
R Moutelikova ◽  
J Prodelalova
Keyword(s):  
Czech Republic ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Phylogenetic Analyses ◽  
Economic Losses ◽  
The Czech Republic ◽  
Nucleocapsid Gene ◽  
Nasal Swabs ◽  
Encephalomyelitis Virus ◽  
Porcine Hemagglutinating Encephalomyelitis Virus

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The incidence of PHEV among pigs in many countries is rising, and the economic losses to the pig industry may be significant. Serological studies suggest that PHEV is spread worldwide. However, no surveillance has been carried out in the Czech Republic. In this study, eight pig farms were screened for the presence of members of the Coronaviridae family with the use of reverse transcription PCR. A collection of 123 faecal samples and 151 nasal swabs from domestic pigs were analysed. In PHEV-positive samples, almost the complete coding sequence of the nucleocapsid gene was amplified and the acquired sequences were compared to those of geographically dispersed PHEV strains; phylogenetic analyses were also performed. PHEV was present in 7.9% of nasal swabs taken from different age categories of pigs. No other swine coronaviruses were detected. The amino acid sequence of the Czech PHEV strains showed 95.8–98.1% similarity to other PHEV reference strains in GenBank. PHEV strains collected from animals on the same farm were identical; however, strains from different farms have only exhibited only 96.7–98.7% amino acid sequence identity. Our study demonstrates the presence of PHEV in pigs in the Czech Republic. The Czech PHEV strains were evolutionarily closest to the Belgium strain VW572.

scholarly journals Differentiation of the Demand for the Hunting Tourism in the Czech Republic

2014 ◽  
Vol 3 (1) ◽  
pp. 27-42
Author(s):  
Šárka Kroupová ◽  
Josef Navrátil ◽  
Kamil Pícha ◽  
Milan Hasman
Keyword(s):  
Czech Republic ◽  
Questionnaire Survey ◽  
Historical Development ◽  
Specific Situation ◽  
The Czech Republic ◽  
Important Thing ◽  
Bohemian Forest ◽  
The World ◽  
Guided Tour ◽  
South Bohemia

AbstractIn the world scale, there are various forms of how the hunting tourism is carried out. there is a considerably specific situation in the czech republic originating in the historical development. the assessment of the diversification of the demand for tourist activities was chosen to be the aim of this article. this assessment is based on a questionnaire survey among tourism participants who consume the specific products of the hunting tourism in the tourist regions of Bohemian Forest and south Bohemia. A guided tour in a game enclosure with a professional commentary and wildlife observation showed to be the most interesting offer. the most important thing is the identification of five main segments of demand called “ordinary” gamekeeper's hunting, “occasional” gamekeeper's hunting, angling activities, hunting without the killing and complementary activities. the differences among the latter five segments were identified in the representation of respondents as far as their gender and their membership in a community of anglers or gamekeepers are concerned.

scholarly journals Two new asexual genera and six new asexual species in the family Microthyriaceae (Dothideomycetes, Ascomycota) from China

MycoKeys ◽  
2021 ◽  
Vol 85 ◽  
pp. 1-30
Author(s):  
Min Qiao ◽  
Hua Zheng ◽  
Ji-Shu Guo ◽  
Rafael F. Castañeda-Ruiz ◽  
Jian-Ping Xu ◽  
...  
Keyword(s):  
New Taxa ◽  
Dna Sequences ◽  
Sequence Data ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Aquatic Hyphomycetes ◽  
Internal Transcribed Spacers ◽  
The Family

The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacers (ITS) and the large subunit (LSU), revealed that these isolates represent eight new taxa in Microthyriaceae, including two new genera, Antidactylariagen. nov. and Isthmomycesgen. nov. and six new species, Antidactylaria minifimbriatasp. nov., Isthmomyces oxysporussp. nov., I. dissimilissp. nov., I. macrosporussp. nov., Triscelophorus anisopterioideussp. nov. and T. sinensissp. nov. These new taxa are described, illustrated for their morphologies and compared with similar taxa. In addition, two new combinations are proposed in this family.

scholarly journals Financial Literacy in the Czech Republic

2015 ◽  
Vol 2 (4) ◽  
pp. 25-35
Author(s):  
Liběna Kantnerová
Keyword(s):  
Young Adults ◽  
Czech Republic ◽  
High Schools ◽  
Young People ◽  
Financial Literacy ◽  
Financial Knowledge ◽  
The Czech Republic ◽  
Youth And Young Adults ◽  
The University ◽  
South Bohemia

This paper analyses the need to deal with the issue of financial literacy and financial knowledge not only by adults, but also by youth and young adults. This paper is focused on research into the knowledge and understanding of the financial literacy of young people, mostly between the ages of 16 to 33 years, via a questionnaire. The survey, undertaken in the Czech Republic, is based on a sample of 329 students from high schools and 329 students from the University of South Bohemia in České Budějovice [658].

scholarly journals Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019)

2019 ◽  
Vol 7 (11) ◽  
pp. 568 ◽  
Author(s):  
Vaclav Hönig ◽  
Martin Palus ◽  
Tomas Kaspar ◽  
Marta Zemanova ◽  
Karolina Majerova ◽  
...  
Keyword(s):  
Czech Republic ◽  
Culex Pipiens ◽  
African Origin ◽  
Southeastern Part ◽  
The Czech Republic ◽  
Usutu Virus ◽  
Turdus Merula ◽  
Diverse Species ◽  
Natural Hosts ◽  
South Bohemia

Usutu virus (USUV) is a flavivirus (Flaviviridae: Flavivirus) of an African origin transmitted among its natural hosts (diverse species of birds) by mosquitoes. The virus was introduced multiple times to Europe where it caused mortality of blackbirds (Turdus merula) and certain other susceptible species of birds. In this study, we report detection of USUV RNA in blackbirds, Culex pipiens and Cx. modestus mosquitoes in the Czech Republic, and isolation of 10 new Czech USUV strains from carcasses of blackbirds in cell culture. Multiple lineages (Europe 1, 2 and Africa 3) of USUV were found in blackbirds and mosquitoes in the southeastern part of the country. A single USUV lineage (Europe 3) was found in Prague and was likely associated with increased mortalities in the local blackbird population seen in this area in 2018. USUV genomic RNA (lineage Europe 2) was detected in a pool of Cx. pipiens mosquitoes from South Bohemia (southern part of the country), where no major mortality of birds has been reported so far, and no flavivirus RNA has been found in randomly sampled cadavers of blackbirds. The obtained data contributes to our knowledge about USUV genetic variability, distribution and spread in Central Europe.

scholarly journals Organic farms in the Czech Republic – Map portal presentation opportunities

2011 ◽  
Vol 57 (No. 12) ◽  
pp. 565-570 ◽  
Author(s):  
J. Vaněk ◽  
I. Brožová ◽  
P. Šimek ◽  
J. Jarolímek ◽  
T. Vogeltanzová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Regional Development ◽  
Pilot Project ◽  
Object Location ◽  
Content Validation ◽  
Organic Farms ◽  
The Czech Republic ◽  
New Perspective ◽  
Map Data ◽  
South Bohemia

  The paper is aimed at presenting the map portal of organic farms in the Czech Republic. The pilot project is concerned with the South Bohemia Region. Extensive map data and resources are displayed by means of a purpose-developed universal software solution called Regional Development Map Portal (RDMP) version 1.0. The database was generated and updated on the basis of detailed content validation and strives for maximum accuracy of map object location. The software solution – apart from supporting all standard functions – represents qualitatively a brand new perspective of map data creation and entails many original elements and functionalities.

Sign in / Sign up

Export Citation Format

Share Document