Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species

Abstract Background Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates. Results Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). blaTEM was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B1 (76.92 %), A (13.85 %) and D (3.08 %) were detected among the isolates. Conclusions Due to the close interaction of horses and humans, these findings would place emphasis on the pathogenic and zoonotic potential of the equine strains and may help to design antimicrobial resistance stewardship programs to control the dissemination of virulent and multi-drug resistant E. coli strains in the community.

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Extraintestinal pathogenic Escherichia coli in Saudi Arabia: A review of antimicrobial resistance and molecular epidemiology

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i2.30 ◽

2020 ◽

Vol 19 (2) ◽

pp. 447-453

Author(s):

Abdulaziz Alqasim

Keyword(s):

Escherichia Coli ◽

Saudi Arabia ◽

Antimicrobial Resistance ◽

Molecular Epidemiology ◽

Bloodstream Infections ◽

Epidemiological Studies ◽

Colistin Resistance ◽

E Coli ◽

Extended Spectrum ◽

Pathogenic Escherichia Coli

Extra-intestinal pathogenic Escherichia coli (ExPEC) is commonly associated with causing urinary tract and bloodstream infections. Over the past two decades, the antimicrobial resistance of ExPEC has increasingly been reported [1]. Given that Saudi Arabia annually hosts mass religious events, such as Hajj, this review investigated several aspects of antimicrobial resistance of ExPEC in this country including the current prevalence of resistance and molecular epidemiology of ExPEC isolates. Generally, the overall prevalence of antibiotic resistance of ExPEC in Saudi Arabia is on increase. The current emergence of colistin resistance in ExPEC represents a major challenge to public health. Local molecular epidemiological studies have shown the dominance of E. coli sequence type 131 (E. coli ST131) over other major ExPEC STs. This is an important observation given that this clone has been associated with high multidrug resistance and extended-spectrum β-lactamases carriage. To reduce the burden of this resistance in the future, it would be crucial to avoid uncontrolled use of antibiotics in either clinical settings or animal food industry. Keywords: Extra-intestinal pathogenic Escherichia coli, Antimicrobial resistance, ST131, Saudi Arabia, Colistin resistance, Extended-spectrum β-lactamases

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Antimicrobial Resistance Profiles of Escherichia coli from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed

Antibiotics ◽

10.3390/antibiotics9110755 ◽

2020 ◽

Vol 9 (11) ◽

pp. 755 ◽

Cited By ~ 1

Author(s):

Do Kyung-Hyo ◽

Byun Jae-Won ◽

Lee Wan-Kyu

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Diffusion Method ◽

Growth Promoters ◽

Disk Diffusion Method ◽

Pathogenic Escherichia Coli ◽

Before And After ◽

Amoxicillin Clavulanic Acid ◽

Antibiotic Growth Promoters ◽

Polymerase Chain

This study aimed to survey the antimicrobial resistance profiles of 690 pathogenic Escherichia coli isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after the ban on antibiotic growth promoters (AGPs). Following the Clinical and Laboratory Standards Institute guidelines, the antimicrobial resistance phenotype was analyzed through the disk diffusion method, and the genotype was analyzed by the polymerase chain reaction. After the ban on AGPs, resistance to gentamicin (from 68.8% to 39.0%), neomycin (from 84.9% to 57.8%), ciprofloxacin (from 49.5% to 39.6%), norfloxacin (from 46.8% to 37.3%), and amoxicillin/clavulanic acid (from 40.8% to 23.5%) decreased compared to before the ban. However, resistance to cephalothin (from 51.4% to 66.5%), cefepime (from 0.0% to 2.4%), and colistin (from 7.3% to 11.0%) had increased. We confirmed a high percentage of multidrug resistance before (95.0%) and after (96.6%) the ban on AGPs. The AmpC gene was the most prevalent from 2007 to 2017 (60.0%), followed by the blaTEM gene (55.5%). The blaTEM was prevalent before (2007–2011, 69.3%) and after (2012–2017, 49.2%) the ban on AGPs. These results provide data that can be used for the prevention and treatment of enteric colibacillosis.

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Patterns of antimicrobial resistance in pathogenic Escherichia coli isolates from cases of calf enteritis during the spring-calving season

Veterinary Microbiology ◽

10.1016/j.vetmic.2014.01.010 ◽

2014 ◽

Vol 170 (1-2) ◽

pp. 73-80 ◽

Cited By ~ 9

Author(s):

James F. Gibbons ◽

Fiona Boland ◽

James F. Buckley ◽

Francis Butler ◽

John Egan ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Pathogenic Escherichia Coli ◽

Calving Season

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Antimicrobial Resistance and Class 1 Integrons in Pathogenic Escherichia coli from Dairy Farms

Foodborne Pathogens and Disease ◽

10.1089/fpd.2005.2.348 ◽

2005 ◽

Vol 2 (4) ◽

pp. 348-352 ◽

Cited By ~ 20

Author(s):

Shelton E. Murinda ◽

Paul D. Ebner ◽

Lien T. Nguyen ◽

Alan G. Mathew ◽

Stephen P. Oliver

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Dairy Farms ◽

Class 1 Integrons ◽

Pathogenic Escherichia Coli ◽

Class 1

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Short communication: Molecular characterization and antimicrobial resistance of pathogenic Escherichia coli isolated from raw milk and Minas Frescal cheeses in Brazil

Journal of Dairy Science ◽

10.3168/jds.2019-16732 ◽

2019 ◽

Vol 102 (12) ◽

pp. 10850-10854 ◽

Cited By ~ 2

Author(s):

J.C. Ribeiro Júnior ◽

F.F. Silva ◽

J.B.A. Lima ◽

E.H. Ossugui ◽

P.I. Teider Junior ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Molecular Characterization ◽

Short Communication ◽

Raw Milk ◽

Pathogenic Escherichia Coli

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A retrospective analysis of antimicrobial resistance in pathogenic Escherichia coli and Salmonella spp. isolates from poultry in Uganda

International Journal of Veterinary Science and Medicine ◽

10.1080/23144599.2021.1926056 ◽

2021 ◽

Vol 9 (1) ◽

pp. 11-21

Author(s):

Steven Kakooza ◽

Adrian Muwonge ◽

Esther Nabatta ◽

Wilfred Eneku ◽

Dickson Ndoboli ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Retrospective Analysis ◽

Salmonella Spp ◽

Pathogenic Escherichia Coli

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Antibiotic and Metal Resistance in Escherichia coli Isolated from Pig Slaughterhouses in the United Kingdom

Antibiotics ◽

10.3390/antibiotics9110746 ◽

2020 ◽

Vol 9 (11) ◽

pp. 746

Author(s):

Hongyan Yang ◽

Shao-Hung Wei ◽

Jon L. Hobman ◽

Christine E. R. Dodd

Keyword(s):

Escherichia Coli ◽

United Kingdom ◽

Antimicrobial Resistance ◽

Metal Resistance ◽

E Coli ◽

The United Kingdom ◽

Important Concern ◽

Pathogenic Escherichia Coli ◽

Amoxicillin Clavulanic Acid ◽

Few Data

Antimicrobial resistance is currently an important concern, but there are few data on the co-presence of metal and antibiotic resistance in potentially pathogenic Escherichia coli entering the food chain from pork, which may threaten human health. We have examined the phenotypic and genotypic resistances to 18 antibiotics and 3 metals (mercury, silver, and copper) of E. coli from pig slaughterhouses in the United Kingdom. The results showed resistances to oxytetracycline, streptomycin, sulphonamide, ampicillin, chloramphenicol, trimethoprim–sulfamethoxazole, ceftiofur, amoxicillin–clavulanic acid, aztreonam, and nitrofurantoin. The top three resistances were oxytetracycline (64%), streptomycin (28%), and sulphonamide (16%). Two strains were resistant to six kinds of antibiotics. Three carried the blaTEM gene. Fifteen strains (18.75%) were resistant to 25 µg/mL mercury and five (6.25%) of these to 50 µg/mL; merA and merC genes were detected in 14 strains. Thirty-five strains (43.75%) showed resistance to silver, with 19 possessing silA, silB, and silE genes. Fifty-five strains (68.75%) were resistant to 8 mM copper or above. Seven contained the pcoE gene. Some strains were multi-resistant to antibiotics, silver, and copper. The results in this study, based on strains isolated between 2007 and 2010, will aid understanding about the effects of strategies to reduce resistance and mechanisms of antimicrobial resistance (AMR).

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