A retrospective analysis of antimicrobial resistance in pathogenic Escherichia coli and Salmonella spp. isolates from poultry in Uganda

Abstract Background Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates. Results Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). blaTEM was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B1 (76.92 %), A (13.85 %) and D (3.08 %) were detected among the isolates. Conclusions Due to the close interaction of horses and humans, these findings would place emphasis on the pathogenic and zoonotic potential of the equine strains and may help to design antimicrobial resistance stewardship programs to control the dissemination of virulent and multi-drug resistant E. coli strains in the community.

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Prevalence and antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from pigs at slaughterhouses in Korea

African Journal of Microbiology Research ◽

10.5897/ajmr10.850 ◽

2011 ◽

Vol 5 (7) ◽

pp. 823-830 ◽

Cited By ~ 6

Author(s):

Bi Kim Hyo ◽

Baek Hyun ◽

Lee SooJin ◽

Jang YangHo ◽

Jung SukChan ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Salmonella Spp

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Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray

The Journal of Microbiology ◽

10.1007/s12275-010-0119-5 ◽

2010 ◽

Vol 48 (5) ◽

pp. 682-688 ◽

Cited By ~ 15

Author(s):

Dong-Hun Kim ◽

Bok-Kwon Lee ◽

Yong-Dae Kim ◽

Sung-Keun Rhee ◽

Young-Chang Kim

Keyword(s):

Escherichia Coli ◽

Yersinia Enterocolitica ◽

Virulence Factor ◽

Salmonella Spp ◽

Factor Gene ◽

Enteropathogenic Bacteria ◽

Pathogenic Escherichia Coli ◽

Shigella Spp ◽

Virulence Factor Gene ◽

Vibrio Spp

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Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps

Canadian Journal of Microbiology ◽

10.1139/w07-019 ◽

2007 ◽

Vol 53 (7) ◽

pp. 919-924 ◽

Cited By ~ 12

Author(s):

Kavitha Boinapally ◽

Xiuping Jiang

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

South Carolina ◽

Antimicrobial Resistance ◽

Pathogenic Bacteria ◽

Vibrio Vulnificus ◽

Resistant Bacteria ◽

Microbiological Analysis ◽

Salmonella Spp ◽

Plate Counts

The objective of this study was to assess and differentiate wild-caught South Carolina (SC) shrimps from imported shrimps on the basis of microbiological analysis. Seven wild-caught SC shrimp and 13 farm-raised imported shrimp samples were analyzed. Total plate counts from wild-caught shrimp samples ranged from 4.3 to 7.0 log10 CFU/g, whereas counts from imported shrimp samples ranged from 3.2 to 5.7 log10 CFU/g. There was no difference (P > 0.05) between total bacterial counts of wild-caught SC shrimp and farm-raised imported shrimp. However, the percentages of bacteria with reduced susceptibility towards ceftriaxone and tetracycline were higher (P < 0.05) for farm-raised shrimp than for wild-caught samples. Salmonella spp. detected only in one farm-raised sample was resistant to ampicillin, ceftriaxone, gentamicin, streptomycin, and trimethoprim. Vibrio vulnificus was detected in both wild-caught and farm-raised shrimp samples; however, only the isolate from farm-raised shrimp was resistant to nalidixic acid and trimethoprim. Escherichia coli detected in one wild-caught sample was resistant to ampicillin. Both Listeria spp. and Salmonella spp. were absent with wild-caught SC samples. Therefore, the presence of more ceftriaxone- and tetracycline-resistant bacteria and the observed antimicrobial resistance phenotypes of isolates from the imported shrimp may reflect the possible use of antibiotics in raising shrimp in those countries.

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Antimicrobial resistance and virulence factors profile of Salmonella spp. and Escherichia coli isolated from different environments exposed to anthropogenic activity

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2020.05.016 ◽

2020 ◽

Vol 22 ◽

pp. 578-583

Author(s):

Michelle M. Balbin ◽

Dawn Hull ◽

Chloe Guest ◽

Lauren Nichols ◽

Robert Dunn ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Virulence Factors ◽

Anthropogenic Activity ◽

Salmonella Spp

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Extraintestinal pathogenic Escherichia coli in Saudi Arabia: A review of antimicrobial resistance and molecular epidemiology

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i2.30 ◽

2020 ◽

Vol 19 (2) ◽

pp. 447-453

Author(s):

Abdulaziz Alqasim

Keyword(s):

Escherichia Coli ◽

Saudi Arabia ◽

Antimicrobial Resistance ◽

Molecular Epidemiology ◽

Bloodstream Infections ◽

Epidemiological Studies ◽

Colistin Resistance ◽

E Coli ◽

Extended Spectrum ◽

Pathogenic Escherichia Coli

Extra-intestinal pathogenic Escherichia coli (ExPEC) is commonly associated with causing urinary tract and bloodstream infections. Over the past two decades, the antimicrobial resistance of ExPEC has increasingly been reported [1]. Given that Saudi Arabia annually hosts mass religious events, such as Hajj, this review investigated several aspects of antimicrobial resistance of ExPEC in this country including the current prevalence of resistance and molecular epidemiology of ExPEC isolates. Generally, the overall prevalence of antibiotic resistance of ExPEC in Saudi Arabia is on increase. The current emergence of colistin resistance in ExPEC represents a major challenge to public health. Local molecular epidemiological studies have shown the dominance of E. coli sequence type 131 (E. coli ST131) over other major ExPEC STs. This is an important observation given that this clone has been associated with high multidrug resistance and extended-spectrum β-lactamases carriage. To reduce the burden of this resistance in the future, it would be crucial to avoid uncontrolled use of antibiotics in either clinical settings or animal food industry. Keywords: Extra-intestinal pathogenic Escherichia coli, Antimicrobial resistance, ST131, Saudi Arabia, Colistin resistance, Extended-spectrum β-lactamases

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Antimicrobial resistance profiles in Salmonella spp. and Escherichia coli isolates from turkey samples in Northern Greece

Annals of Microbiology ◽

10.1007/s13213-011-0299-1 ◽

2011 ◽

Vol 62 (2) ◽

pp. 623-628 ◽

Cited By ~ 3

Author(s):

Eleni G. Iossifidou ◽

Amin Abrahim ◽

Nikolaos D. Soultos ◽

Eleftherios A. Triantafillou ◽

Pavlos A. Koidis

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Salmonella Spp ◽

Northern Greece

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Antimicrobial Resistance Profiles of Escherichia coli from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed

Antibiotics ◽

10.3390/antibiotics9110755 ◽

2020 ◽

Vol 9 (11) ◽

pp. 755 ◽

Cited By ~ 1

Author(s):

Do Kyung-Hyo ◽

Byun Jae-Won ◽

Lee Wan-Kyu

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Diffusion Method ◽

Growth Promoters ◽

Disk Diffusion Method ◽

Pathogenic Escherichia Coli ◽

Before And After ◽

Amoxicillin Clavulanic Acid ◽

Antibiotic Growth Promoters ◽

Polymerase Chain

This study aimed to survey the antimicrobial resistance profiles of 690 pathogenic Escherichia coli isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after the ban on antibiotic growth promoters (AGPs). Following the Clinical and Laboratory Standards Institute guidelines, the antimicrobial resistance phenotype was analyzed through the disk diffusion method, and the genotype was analyzed by the polymerase chain reaction. After the ban on AGPs, resistance to gentamicin (from 68.8% to 39.0%), neomycin (from 84.9% to 57.8%), ciprofloxacin (from 49.5% to 39.6%), norfloxacin (from 46.8% to 37.3%), and amoxicillin/clavulanic acid (from 40.8% to 23.5%) decreased compared to before the ban. However, resistance to cephalothin (from 51.4% to 66.5%), cefepime (from 0.0% to 2.4%), and colistin (from 7.3% to 11.0%) had increased. We confirmed a high percentage of multidrug resistance before (95.0%) and after (96.6%) the ban on AGPs. The AmpC gene was the most prevalent from 2007 to 2017 (60.0%), followed by the blaTEM gene (55.5%). The blaTEM was prevalent before (2007–2011, 69.3%) and after (2012–2017, 49.2%) the ban on AGPs. These results provide data that can be used for the prevention and treatment of enteric colibacillosis.

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