Bovine mastitis may be associated with the deprivation of gut Lactobacillus

2016 ◽  
Vol 7 (1) ◽  
pp. 95-102 ◽  
Author(s):  
C. Ma ◽  
J. Zhao ◽  
X. Xi ◽  
J. Ding ◽  
H. Wang ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Microbial Community ◽  
Somatic Cell Count ◽  
Close Association ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Lactobacillus Species ◽  
General Increase ◽  
Community Changes ◽  
Mastitis Pathogens

Bovine mastitis is an economical important microbial disease in dairy industry. Some recent human clinical trials have shown that oral probiotics supplementation could effectively control clinical mastitis, suggesting that the mechanism of mastitis protection might be achieved via the host gut microbiota. We aimed to test our hypothesis that bovine mastitis was related to changes in both the mammary and gut microbial profiles. By quantitative PCR, the milk and faecal microbial profiles of cows with low (<3×105 cells/ml) and high (>1×106 cells/ml) somatic cell count (SCC) were compared. Firstly, we observed drastic differences in both the milk and faecal microbial compositions at genus and Lactobacillus-species levels between the two groups. Secondly, the pattern of faecal microbial community changes of mastitis cows was similar to that of the milk, characterised by a general increase in the mastitis pathogens (Enterococcus, Streptococcus and Staphylococcus) and deprivation of Lactobacillus and its members (L. salivarius, L. sakei, L. ruminis, L. delbrueckii, L. buchneri, and L. acidophilus). Thirdly, only the faecal lactobacilli, but not bifidobacteria correlated with the milk microbial communities and SCC. Our data together hint to a close association between bovine mastitis, the host gut and milk microbiota.

scholarly journals Prevalence of bovine mastitis pathogens and antibioresistance of Escherichia coli isolates

2017 ◽  
Vol 55 (2) ◽  
pp. 113
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ) ◽  
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)
Keyword(s):  
Escherichia Coli ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Resistance Rate ◽  
Northern Greece ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Mastitis Pathogens

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.

N-acetyl -β-D-glucosaminidase activity in cow milk as an indicator of mastitis

2016 ◽  
Vol 83 (2) ◽  
pp. 219-227 ◽  
Author(s):  
Mari Hovinen ◽  
Heli Simojoki ◽  
Reeta Pösö ◽  
Jenni Suolaniemi ◽  
Piret Kalmus ◽  
...  
Keyword(s):  
Somatic Cell Count ◽  
Bovine Mastitis ◽  
Reference Value ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cow Milk ◽  
Milk Samples ◽  
High Level ◽  
Accurate Indicator

Activity of lysosomal N-acetyl-β-d-glucosaminidase (NAGase) in milk has been used as an indicator of bovine mastitis. We studied NAGase activity of 808 milk samples from healthy quarters and quarters of cows with spontaneous subclinical and clinical mastitis. Associations between milk NAGase activity and milk somatic cell count (SCC), mastitis causing pathogen, quarter, parity, days in milk (DIM) and season were studied. In addition, the performance of NAGase activity in detecting clinical and subclinical mastitis and distinguishing infections caused by minor and major bacteria was investigated. Our results indicate that NAGase activity can be used to detect both subclinical and clinical mastitis with a high level of accuracy (0·85 and 0·99). Incomplete correlation between NAGase activity and SCC suggests that a substantial proportion of NAGase activity comes from damaged epithelial cells of the udder in addition to somatic cells. We therefore recommend determination of NAGase activity from quarter foremilk after at least six hours from the last milking using the method described. Samples should be frozen before analysis. NAGase activity should be interpreted according to DIM, at least during the first month of lactation. Based on the results of the present study, a reference value for normal milk NAGase activity of 0·1–1·04 pmoles 4-MU/min/μl for cows with ≥30 DIM (196 samples) could be proposed. We consider milk NAGase activity to be an accurate indicator of subclinical and clinical mastitis.

scholarly journals Geraniol, a Potential Alternative to Antibiotics for Bovine Mastitis Treatment Without Disturbing the Host Microbial Community, Does Not Lead to Drug Residues or Induce Drug Resistance

2021 ◽  
Author(s):  
Guo Wei ◽  
Min Qiu ◽  
Zhonghui Pu ◽  
Nana Long ◽  
Min Yang ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Microbial Community ◽  
Gut Microbiome ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Inflammatory Factors ◽  
Drug Residues ◽  
Potential Alternative

Abstract BackgroundMastitis is one of the important diseases of the dairy cow. Currently, mastitis treatment in dairy cows is mainly based on antibiotics. However, the use of antibiotics causes adverse effects, including drug resistance, drug residues, host-microbiome destruction, and environmental pollution. Geraniol, extracted from Fructus Tsaoko, has demonstrated good antibacterial activity in mouse model. Geraniol and antibiotics were used to treat cows with clinical mastitis to test this possibility of geraniol as a potential alternative to antibiotics for bovine mastitis treatment. The effectiveness of treatment, improvement in inflammatory factors, the influence on microbiome, presence of drug residues, and induction of drug resistance were compared and analyzed.ResultsGeraniol demonstrated a better therapeutic rate than antibiotics on clinical mastitis of cows, with a longer course of treatment. Antibiotics and geraniol significantly reduced the abundance of pathogenic bacteria and restored the microbial community in milk. Meanwhile, geraniol increased the abundance of probiotics in milk. Interestingly, geraniol did not destroy the gut microbial community of cows, whereas antibiotics significantly reduced the diversity and destroyed the community structure of the gut microbiome in cows. Conversely, geraniol increased the diversity of the gut microbiome. Besides, no geraniol residue was detected in the milk four days after treatment discontinuation. However, antibiotic residues were detected in milk at the 7th day after drug withdrawal. In vitro experiments revealed that geraniol did not induce drug resistance in the Escherichia coli strain ATCC25922 after 120 generations of culturing, while antibiotics induced resistance after 10 generations. ConclusionsOur results suggest that geraniol has antibacterial and anti-inflammatory effects similar to antibiotics. Additionally, it retains the structure of the host-microbial community and does not lead to drug residues or induce drug resistance. Therefore, geraniol can be a potential substitute for antibiotics to treat mastitis and be widely used in the dairy industry.

scholarly journals Diagnosis of clinical and subclinical mastitis in a rural property in Carambeí, State of Paraná

2021 ◽  
Vol 10 (3) ◽  
pp. e32310313411
Author(s):  
Djalma Cesar Clock ◽  
Gislaine Gabardo ◽  
Josiel Rodrigues da Luz ◽  
Giovani Mansani de Araujo Avila
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Somatic Cell Count ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Laboratory Analysis ◽  
The Other ◽  
California Mastitis Test ◽  
Bacteria And Fungi

The aim of the present study was to diagnose clinical and subclinical bovine mastitis in a property in the municipality of Carambeí-PR. 86 dairy cows were diagnosed in two evaluations carried out on July 23 and November 4, 2020. The detection of clinical mastitis was performed by testing the black-bottomed mug, whereas the detection of animals with sub-clinical mastitis by the California Mastitis Test (CMT) and laboratory analysis of bacteria and fungi present. In the first evaluation, 9.4% of the animals had mastitis, while in the second 5.16% of the herd. The milk of the animals that tested positive were submitted to laboratory analysis. In the first evaluation, they were diagnosed with an incidence of 15% Escherichia coli, 15% Prothoteca / yeast, 23% Staphlococcus aureus, 46% Streptococcus agalactiae. In the second evaluation, there was an incidence of 25% Enterococcus sp. and 25% S. agalactiae, the other animals showed 50% Staphlococcus non aureus. There was a higher occurrence of subclinical mastitis in the property, in both evaluations. There was a reduction from the first to the second evaluation in the incidence of cases of subclinical mastitis and the cases of clinical mastitis were resolved. Contributing to the lower disposal of milk caused by the high somatic cell count.

scholarly journals Influence of nutrient availability on in vitro growth of major bovine mastitis pathogens

2021 ◽  
Vol 88 (1) ◽  
pp. 80-88
Author(s):  
Remo Stürmlin ◽  
Josef J. Gross ◽  
Olga Wellnitz ◽  
Lea A. Wagner ◽  
Camille Monney ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Nutrient Availability ◽  
Bovine Mastitis ◽  
Milk Composition ◽  
B Vitamins ◽  
E Coli ◽  
Streptococcus Uberis ◽  
Whole Milk ◽  
Mastitis Pathogens

AbstractThe aim of the present study was to investigate the effects of milk composition changes on the in vitro growth of bovine mastitis pathogens. Nutritional requirements of three major bovine mastitis pathogens Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Streptococcus uberis (S. uberis) were investigated in vitro. We used ultra-high temperature (UHT) treated milk with different contents of fat, protein, and carbohydrates to test the influence of the availability of various milk constituents on pathogen growth characteristics. Additionally, the bacterial growth was investigated under experimentally modified nutrient availability by dilution and subsequent supplementation with individual nutrients (carbohydrates, different nitrogen sources, minerals, and different types of B vitamins) either to milk or to a conventional medium (thioglycolate broth, TB). Varying contents of fat, protein or lactose did not affect bacterial growth with the exception of growth of S. uberis being promoted in protein-enriched milk. The addition of nutrients to diluted whole milk and TB partly revealed different effects, indicating that there are media-specific growth limiting factors after dilution. Supplementation of minerals to diluted milk did not affect growth rates of all studied bacteria. Bacterial growth in diluted whole milk was decreased by the addition of high concentrations of amino acids in S. aureus, and by urea and additional B vitamins in E. coli and S. aureus. The growth rate of S. uberis was increased by the addition of B vitamins to diluted whole milk. The present results demonstrate that growth-limiting nutrients differ among pathogen types. Because reduced bacterial growth was only shown in diluted milk or TB, it is unlikely that alterations in nutrient availability occurring as a consequence of physiological changes of milk composition in the cow's udder would directly affect the susceptibility or course of bovine mastitis.

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