scholarly journals Beneficial effects of carotenoid-producing cells of Bacillus indicus HU16 in a rat model of diet-induced metabolic syndrome

2017 ◽  
Vol 8 (5) ◽  
pp. 823-831 ◽  
Author(s):  
R. Crescenzo ◽  
A. Mazzoli ◽  
R. Cancelliere ◽  
A. Bucci ◽  
G. Naclerio ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Gut Microbiota ◽  
Oral Administration ◽  
Rat Model ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Standard Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Beneficial Effects ◽  
Markers Of Inflammation

A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects of the oral administration of spores or cells of HU16, a carotenoid-producing strain of Bacillus indicus. Symptoms of metabolic syndrome were induced in 90-days old, male Sprague-Dawley rats maintained for eight weeks on a high-fat diet, as previously reported. Parallel groups of animals under the same diet regimen also received a daily dose of 1×1010 cells or spores of B. indicus HU16. Cells of strain HU16 were able to reduce symptoms of metabolic syndrome, plasma markers of inflammation and oxidative markers in plasma and liver to levels similar to those observed in rats under a standard diet. HU16 cells did not affect obesity markers or the accumulation of triglycerides in the liver of treated animals. Denaturing gradient gel electrophoresis analysis showed that the oral administration of HU16 cells did not significantly affect the gut microbiota of high fat-fed rats, suggesting that the observed beneficial effects are not due to a reshaping of the gut microbiota but rather to metabolites produced by HU16 cells.

scholarly journals Effects of combined d-fagomine and omega-3 PUFAs on gut microbiota subpopulations and diabetes risk factors in rats fed a high-fat diet

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mercè Hereu ◽  
Sara Ramos-Romero ◽  
Cristina Busquets ◽  
Lidia Atienza ◽  
Susana Amézqueta ◽  
...  
Keyword(s):  
Risk Factors ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Short Chain Fatty Acids ◽  
Standard Diet ◽  
Liver Inflammation ◽  
Omega 3 ◽  
Sprague Dawley ◽  
High Fat ◽  
Related Risk

Abstract Food contains bioactive compounds that may prevent changes in gut microbiota associated with Westernized diets. The aim of this study is to explore the possible additive effects of d-fagomine and ω-3 PUFAs (EPA/DHA 1:1) on gut microbiota and related risk factors during early stages in the development of fat-induced pre-diabetes. Male Sprague Dawley (SD) rats were fed a standard diet, or a high-fat (HF) diet supplemented with d-fagomine, EPA/DHA 1:1, a combination of both, or neither, for 24 weeks. The variables measured were fasting glucose and glucose tolerance, plasma insulin, liver inflammation, fecal/cecal gut bacterial subgroups and short-chain fatty acids (SCFAs). The animals supplemented with d-fagomine alone and in combination with ω-3 PUFAs accumulated less fat than those in the non-supplemented HF group and those given only ω-3 PUFAs. The combined supplements attenuated the high-fat-induced incipient insulin resistance (IR), and liver inflammation, while increasing the cecal content, the Bacteroidetes:Firmicutes ratio and the populations of Bifidobacteriales. The functional effects of the combination of d-fagomine and EPA/DHA 1:1 against gut dysbiosis and the very early metabolic alterations induced by a high-fat diet are mainly those of d-fagomine complemented by the anti-inflammatory action of ω-3 PUFAs.

scholarly journals Hypertension Programmed by Perinatal High-Fat Diet: Effect of Maternal Gut Microbiota-Targeted Therapy

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2908 ◽  
Author(s):  
Chien-Ning Hsu ◽  
Chih-Yao Hou ◽  
Julie Y.H. Chan ◽  
Chien-Te Lee ◽  
You-Lin Tain
Keyword(s):  
Gut Microbiota ◽  
Lactobacillus Casei ◽  
Renin Angiotensin System ◽  
Short Chain Fatty Acids ◽  
Lactobacillus Species ◽  
Sprague Dawley ◽  
High Fat ◽  
Beneficial Effects ◽  
Therapeutic Uses ◽  
Pregnancy And Lactation

Hypertension can originate in early life caused by perinatal high-fat (HF) consumption. Gut microbiota and their metabolites short chain fatty acids (SCFAs), trimethylamine (TMA), and trimethylamine N-oxide (TMAO) are involved in the development of hypertension. Despite the beneficial effects of prebiotic/probiotic on human health, little is known whether maternal use of prebiotics/probiotics could protect offspring against the development of hypertension in adulthood. We investigated whether perinatal HF diet-induced programmed hypertension in adult offspring can be prevented by therapeutic uses of prebiotic inulin or probiotic Lactobacillus casei during gestation and lactation. Pregnant Sprague–Dawley rats received regular chow or HF diet (D12331, Research Diets), with 5% w/w long chain inulin (PRE), or 2 × 108 CFU/day Lactobacillus casei via oral gavage (PRO) during pregnancy and lactation. Male offspring (n = 8/group) were assigned to four groups: control, HF, PRE, and PRO. Rats were sacrificed at 16 weeks of age. Maternal prebiotic or probiotic therapy prevents elevated blood pressure (BP) programmed by perinatal HF consumption. Both prebiotic and probiotic therapies decreased the Firmicutes to Bacteroidetes ratio and renal mRNA expression of Ace, but increased abundance of genus Lactobacillus and Akkermansia. Additionally, prebiotic treatment prevents HF-induced elevation of BP is associated with reduced fecal propionate and acetate levels, while probiotic therapy restored several Lactobacillus species. Maternal probiotic or prebiotic therapy caused a reduction in plasma TMAO level and TMAO-to-TMA ratio. The beneficial effects of prebiotic or probiotic therapy on elevated BP programmed by perinatal HF diet are relevant to alterations of microbial populations, modulation of microbial-derived metabolites, and mediation of the renin-angiotensin system. Our results cast a new light on the use of maternal prebiotic/probiotic therapy to prevent hypertension programmed by perinatal HF consumption. The possibility of applying gut microbiota-targeted therapies as a reprogramming strategy for hypertension warrants further clinical translation.

scholarly journals PENGARUH SINBIOTIK KEFIR TEPUNG PISANG BATU (Musa balbisiana) TERHADAP KADAR GLUKOSA DARAH TIKUS SINDROM METABOLIK

2020 ◽  
Vol 7 (1) ◽  
pp. 18-27
Author(s):  
Dhea Marliana Salsabila ◽  
Taufik Maryusman ◽  
Iin Fatmawati
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Negative Control ◽  
Standard Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Musa Balbisiana ◽  
Significant Difference

Metabolic syndrome begins with insulin resistance characterized by hyperglycemia. Synbiotic kefir banana (Musa balbisiana Colla) flour reduces blood glucose level. This study was conducted to analyze the effects of synbiotic kefir banana (M. balbisiana) flour on blood glucose level of metabolic syndrome rats. This study used 24 Sprague Dawley.rats which were divided into four groups, i.e. negative control was given standard diet, positive control was given high fat fructose diet (HFFD), treatment I (PI) and treatment II (PII) were given HFFD and synbiotic kefir banana (M. balbisiana) flour 1.8 mL 200 g־¹ rat BW per day (PI) and 3.6 mL 200 g־¹ rat BW per day (PII), respectively, for three weeks. The result showed a significant difference (p=0.000) in blood glucose after giving synbiotic kefir banana (M. balbisiana) flour. Synbiotic kefir banana (M. balbisiana) flour reduced blood glucose level in metabolic syndrome rats.Keywords: banana flour, blood glucose level, kefir, metabolic syndrome, synbiotic ABSTRAKSindrom metabolik diawali resistensi insulin yang ditandai hiperglikemia. Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah. Penelitian ini dilakukan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (M. balbisiana) terhadap kadar glukosa darah tikus sindrom metabolik. Penelitian ini menggunakan 24 tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif yang diberi pakan standar, kontrol positif yang diberi high fat fructose diet (HFFD), dan perlakuan I (PI) serta perlakuan II (PII) yang masing-masing diberi HFFD dan sinbiotik kefir tepung pisang batu (M. balbisiana) 1,8 mL 200 g־¹ BB tikus per hari (PI) dan 3,6 mL 200 g־¹ BB tikus per hari (PII) selama tiga minggu. Hasil menunjukkan perbedaan kadar glukosa darah setelah pemberian sinbiotik kefir tepung pisang batu (M. balbisiana) secara signifikan (p=0,000). Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah tikus sindrom metabolik.

Beneficial effects of flaxseed polysaccharides on metabolic syndrome via gut microbiota in high-fat diet fed mice

2020 ◽  
Vol 131 ◽  
pp. 108994 ◽  
Author(s):  
Chen Yang ◽  
Zhenxia Xu ◽  
Qianchun Deng ◽  
Qingde Huang ◽  
Xu Wang ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
High Fat ◽  
Beneficial Effects

IMMUNOLOGICAL ASPECT OF ORAL ADMINISTRATION OF THE PROBIOTIC LACTOBACILLUS CASEI LB 148 UNDER COLD STRESS IN ANIMALS

2020 ◽  
Author(s):  
G.V. Kozlovskaya ◽  
◽  
M.I. Zinevich ◽  
Y.E. Kozlovsky ◽  
T.I. Khomyakova ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Oral Administration ◽  
Cold Stress ◽  
Gut Microbiome ◽  
Lactobacillus Casei ◽  
Causative Factor ◽  
Sprague Dawley ◽  
Immunological Aspect ◽  
Intestinal Lymph ◽  
Probiotic Lactobacillus

Gut microbiome changes is considered as a basic causative factor of stress-associated diseases. Probiotics are usually used for the correction of disbiosis. The aim of the investigation was the study of the effects of oral probiotic Lactobacillus casei LB 148 use onto the gut microbiota as well as the number and total square of intestinal lymph nodules of rats Sprague Dawley at in health rats and under the cold stress

Polysaccharide from Flammulina velutipes Attenuates Markers of Metabolic Syndrome by modulating the Gut Microbiota and Lipid Metabolism in High fat Diet-fed Mice

2021 ◽  
Author(s):  
Ruiqiu Zhao ◽  
Yang Ji ◽  
Xin Chen ◽  
Qiuhui Hu ◽  
Liyan Zhao
Keyword(s):  
Metabolic Syndrome ◽  
Lipid Metabolism ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Flammulina Velutipes ◽  
Biological Macromolecules ◽  
High Fat

Natural biological macromolecules with putative functions of gut microbiota regulation possesses the advantage in improving metabolic syndrome (MS). In this research, we aimed to determine the effects of Flammulina velutipes...

The ameliorative effect of the Pyracantha fortuneana (Maxim.) H. L. Li extract on intestinal barrier dysfunction through modulating glycolipid digestion and gut microbiota in high fat diet-fed rats

2019 ◽  
Vol 10 (10) ◽  
pp. 6517-6532 ◽  
Author(s):  
Hang Xu ◽  
Chunfang Zhao ◽  
Yutian Li ◽  
Ruiyu Liu ◽  
Mingzhang Ao ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Intestinal Barrier ◽  
High Fat ◽  
Barrier Dysfunction ◽  
Fruit Extract ◽  
Intestinal Barrier Dysfunction ◽  
Beneficial Effects ◽  
Obese Rats ◽  
Ameliorative Effect

Pyracantha fortuneana fruit extract (PFE) exhibits beneficial effects on IBF in association with the modulation of glycolipid digestion and gut microbiota in HFD-fed obese rats.

Discovery of Serum Proteomic Biomarkers for Prediction of Response to Moxibustion Treatment in Rats with Collagen-Induced Arthritis: An Exploratory Analysis

2016 ◽  
Vol 34 (3) ◽  
pp. 184-193 ◽  
Author(s):  
Xiao Xu ◽  
Miao-Miao Wang ◽  
Zhi-ling Sun ◽  
Dan-ping Zhou ◽  
Ling Wang ◽  
...  
Keyword(s):  
Rat Model ◽  
Multiple Testing ◽  
Day Treatment ◽  
Expression Patterns ◽  
Control Group ◽  
Sprague Dawley ◽  
Collagen Induced Arthritis ◽  
Serum Samples ◽  
Beneficial Effects ◽  
Bovine Collagen

Objective To examine the possible impact of moxibustion on the serum proteome of the collagen-induced arthritis (CIA) rat model. Materials and Methods Thirty-six male Sprague-Dawley rats were included in this experiment. The CIA animal model was prepared by injection of type II bovine collagen in Freund's adjuvant on the first and seventh day. The 36 rats were randomly divided into two groups: the untreated CIA group (control), and the CIA plus treatment with moxibustion (CIA+moxi) group. Moxibustion was administered daily at ST36 and BL23 for 7, 14 or 21 days (n=12 rats each). Arthritis score was used to assess the severity of arthritis. At the end of each 7 day treatment, blood samples from the control group and the CIA+moxi group were collected. After removal of high abundance proteins from serum samples, two-dimensional gel combined with matrix-assisted laser desorption ionisation time-of-flight MS/MS (MALDI-TOF-MS/MS) techniques were performed to examine serum protein expression patterns of the CIA rat model with and without moxibustion treatment. In addition, the relevant proteins were further analysed with the use of bioinformatics analysis. Results Moxibustion significantly decreased arthritis severity in the rats in the CIA+moxi group, when compared with the rats in the CIA group 35 days after the first immunisation (p=0.001). Seventeen protein spots which changed >1.33 or <0.77 at p<0.05 using Bonferonni correction for multiple testing were found to be common to all three comparisons, and these proteins were used for classification of functions using the Gene Ontology method. Consequently, with the use of the Ingenuity Pathway Analysis, the top canonical pathways and a predicted proteomic network related to the moxibustion effect of CIA were established. Conclusions Using the proteomics technique, we have identified novel candidate proteins that may be involved in the mechanisms of action underlying the beneficial effects of moxibustion in rats with CIA. Our findings suggest that immune responses and metabolic processes may be involved in mediating the effects of moxibustion. Moreover, periodxiredoxin I (PRDX1) and inositol 1,4,5-triphosphate receptor (IP3R) may be potential targets.

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