scholarly journals Functional modulation of caecal fermentation and microbiota in rat by feeding bean husk as a dietary fibre supplement

2018 ◽  
Vol 9 (6) ◽  
pp. 963-974 ◽  
Author(s):  
H. Myint ◽  
H. Kishi ◽  
Y. Iwahashi ◽  
W. Saburi ◽  
S. Koike ◽  
...  
Keyword(s):  
Clone Library ◽  
Corn Starch ◽  
Ammonia Level ◽  
Soya Bean ◽  
Gut Health ◽  
Sprague Dawley ◽  
Clone Library Analysis ◽  
Sprague Dawley Rats ◽  
Caecal Fermentation ◽  
Anatomical Parameters

A feeding study using rats was conducted to evaluate the utility of lablab bean husk and soya bean husk as sources of potential prebiotic fibre. Twenty 5-week-old Sprague Dawley rats were divided into 4 groups and fed one of the following diets for 3 weeks: purified diet (AIN93 G) containing 5% cellulose (CEL), or the same diet in which cellulose was replaced by corn starch (STA), lablab bean husk (LBH), or soya bean husk (SBH). Rats were sacrificed at 8 weeks of age and caecal digesta were collected. Feed intake, body weight, anatomical parameters, and caecal ammonia level did not differ significantly among diets. Rats on LBH and SBH showed higher concentrations of caecal short-chain fatty acid and lactate than those on CEL. Rats on CEL, SBH, and LBH exhibited lower caecal indole and skatole levels. LBH yielded increased caecal abundance of Akkermansia muciniphila and Oscillibacter relatives, as demonstrated by either qPCR, MiSeq, or clone library analysis. SBH favoured the growth of lactobacilli as assessed by both qPCR and MiSeq, and favoured the growth of bifidobacteria as assessed by MiSeq. In comparison with STA, LBH and SBH yielded lower caecal abundance of bacteria related to Dorea massiliensis, as demonstrated by qPCR, MiSeq, and clone library analysis. Both types of bean husk were found to contain oligosaccharides that might selectively stimulate the growth of beneficial bacteria. Based on these results, the two species of bean husk tested are considered potentially functional for promoting the gut health of monogastric animals.

A 90-day oral (dietary) toxicity and mass balance study of corn starch fiber in Sprague Dawley rats

2016 ◽  
Vol 97 ◽  
pp. 57-69 ◽  
Author(s):  
Christine M. Crincoli ◽  
Andrey I. Nikiforov ◽  
Marisa O. Rihner ◽  
Elizabeth A. Lambert ◽  
Melanie A. Greeley ◽  
...  
Keyword(s):  
Mass Balance ◽  
Corn Starch ◽  
Sprague Dawley ◽  
Balance Study ◽  
Sprague Dawley Rats ◽  
Dietary Toxicity ◽  
Mass Balance Study

scholarly journals Antiobesity Activity of Elateriospermum tapos Shell Extract in Obesity-Induced Sprague Dawley Rats

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 321
Author(s):  
Kokila Vani Perumal ◽  
Nor Liyana Ja’afar ◽  
Che Norma Mat Taib ◽  
Nurul Husna Shafie ◽  
Hasnah Bahari
Keyword(s):  
Body Weight ◽  
Corn Starch ◽  
Milk Powder ◽  
Caloric Intake ◽  
Lipid Lowering ◽  
Sprague Dawley ◽  
Triglyceride Accumulation ◽  
Sprague Dawley Rats ◽  
Effective Result ◽  
Lipid Lowering Effect

Obesity is one of the risk factors associated with cardiovascular diseases, hypertension, abnormal liver function, diabetes, and cancers. Orlistat is currently available to treat obesity, but it is associated with adverse side effects. Natural resources are widely used for obesity treatment. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. The rats’ obesity was induced by a high-fat (HF) diet made up of 50% standard rat pellet, 20% milk powder, 6% corn starch, and 24% ghee and a cafeteria (CAF) diet such as chicken rolls, salty biscuits, cakes, and cheese snacks. A hot aqueous method for the extraction of E. tapos shells was applied by using 500 mL of distilled water for about 24 h. Various dosages of E. tapos shell extract (10 mg/kg, 100 mg/kg, and 200 mg/kg) were used. At the end of the study, body weight, caloric intake, organ weight, lipid profile, lipoprotein lipase (LPL) activity, and histopathology analysis were carried out. E. tapos shell extract treated groups showed a reduction in body weight, positive lipid-lowering effect, decrements in triglyceride accumulation and LPL activity, and positive improvement in histopathology analysis. A dose of 200 mg/kg showed the most effective result compared to 10 mg/kg and 100 mg/kg doses.

scholarly journals The effect of genetically modified Lactobacillus plantarum 590 on the gut health of sprague-dawley rats

IUBMB Life ◽  
2012 ◽  
Vol 64 (7) ◽  
pp. 617-627 ◽  
Author(s):  
Hai-Yan Liu ◽  
Wen-Tao Xu ◽  
Yan-Fang Yuan ◽  
Si-Shuo Cao ◽  
Xiao-Yun He ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Genetically Modified ◽  
Gut Health ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Fermentation of Resistant Starch Is Not a Predictor of Very High or Low Serum TMAO in Rats, but Results in Greater Serum TMAO than Controls

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 386-386
Author(s):  
Diana Coulon ◽  
Ryan Page ◽  
Justin Guice ◽  
Anne Raggio ◽  
Brian Marx ◽  
...  
Keyword(s):  
Resistant Starch ◽  
Control Diet ◽  
Human Study ◽  
Cleveland Clinic ◽  
Mass Spectrometry Data ◽  
Gut Health ◽  
Sprague Dawley ◽  
High Fat ◽  
Risk Of Death ◽  
Sprague Dawley Rats

Abstract Objectives Consumption of resistant starch (RS) has positive gut health benefits, but RS has been associated with increased serum trimethylamine oxide (TMAO). Increased serum TMAO has been linked to enhanced risk of cardiovascular death. A human study found that RS and a high fat (41% of energy) diet resulted in higher TMAO levels compared to a low RS diet, but this was not seen in a high carbohydrate diet (27% energy as fat). We wanted to ascertain serum TMAO in rats fed a high-RS diet with moderate (MF) or high fat (HF) diets compared to controls. Methods Twenty-four male, Sprague-Dawley rats (n = 6) were fed either 23% RS type 2, high-amylose diet or a control diet using 100% amylopectin with either MF (30% energy) or HF (42% energy) levels. A 2X choline amount had been used in the AIN-93M diets as substrate for ultimate production of TMAO. After six weeks, serum TMAO was measured using stable isotope dilution liquid chromatography tandem mass spectrometry. Data were analyzed as a 2 × 2 factorial followed by Tukey test (P < 0.05). Serum TMAO is assessed for risk of death over a seven-year period into quadrants. Quadrants 3 (4.28–7.89 µM) and 4 (7.91-186.1 µM) have greater risk. Quadrants 1 (0.12–2.55 µM) and 2 (2.56–4.27 µM) had much lower risk. Results Average TMAO level for rats fed RS HF was 2.94 ± 0.4, and RS MF was 2.42 ± 0.4. The control HF was 1.53 ± 0.17, and control MF was 1.06 ± 0.17. Rats fed RS had significantly greater TMAO levels of 2.68 ± 0.28, quadrant 2, while the control rats had an average of 1.3 ± 0.12, quadrant 1. There was no significant increased effect for MF vs. HF. However, the variability was similar for the two RS groups with values in quadrants 1–3. Amount of fermentation was not associated with TMAO amounts. For example, the RS rat with the lowest serum TMAO (0.87 µM) had an empty cecum weight (ECW) of 2.40 g, while the highest (4.41 µM) had an ECW of 2.29 g. Conclusions Sprague-Dawley rats fed either MF or HF had increased TMAO with consumption of RS. Data indicate amount of fermentation is not the reason for increased TMAO. Possible differences in gut bacteria that produce trimethylamine from choline may be cause of TMAO production in the rats. Funding Sources LSU AgCenter and Lerner Research Institute (Dr Stan Hazen) of Cleveland Clinic for analysis of serum TMAO, and starches were gifted from Ingredion Incorporated.

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

Freeze-etch studies of epiphyseal growth plate cartilage reveal matrix vesicles associated with calcification

1978 ◽  
Vol 36 (2) ◽  
pp. 670-671
Author(s):  
Russell N. A. Cecil ◽  
H. Clarke Anderson
Keyword(s):  
Chromic Acid ◽  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Matrix Vesicle ◽  
Glycerol Solution ◽  
Sprague Dawley ◽  
Epiphyseal Growth Plate ◽  
Growth Plates ◽  
Sprague Dawley Rats ◽  
Tibial Epiphyseal

Unfixed proximal tibial epiphyseal growth plates were studied by freeze-etch to confirm the presence of extracellular calcifying matrix vesicles and to determine the substructure of matrix vesicle membranes as compared to plasma and other membranes of intact chondrocytes. Growth plates from 6-10 week old Sprague-Dawley rats were cut into 1x3 mm blocks whose long dimension was oriented either perpendicular or parallel to the long axis of the tibia. Some blocks were fixed at pH 7. 0 in 0. 2M cacodylate - buffered 2. 5% glutaraldehyde for 1 hour at 4ÅC. The blocks were immersed in 30% glycerol solution at 4ÅC for 1 hour, frozen in liquid nitrogen, and then fractured, etched for 2 minutes, and coated with platinum, carbon and 0. 2% Formvar solution. The replicas were cleaned with chromic acid, floated onto Formvar coated grids, and examined with a Phillips EM 300 electron microscope.Fixed and unfixed specimens appeared similar in ultrastructure. Chondrocytes, matrix, and matrix vesicles were identified. In specimens fractured parallel to the long axis of the tibia, the reserve, proliferative, hypertrophic, and calcifying zones could be discerned as described by light and electron microscopy.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

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