Protein composition and digestibility of black soldier fly larvae in broiler chickens revisited according to the recent nitrogen-protein conversion ratio

2018 ◽  
Vol 4 (3) ◽  
pp. 171-177 ◽  
Author(s):  
J. Nery ◽  
L. Gasco ◽  
S. Dabbou ◽  
A. Schiavone
Keyword(s):  
Broiler Chickens ◽  
Weighted Average ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Adaptation Period ◽  
Protein Nitrogen ◽  
Black Soldier Fly ◽  
Conversion Factors ◽  
The Mean ◽  
Poultry Diets

Specific nitrogen-to-protein (N:P) conversion factors have recently been defined for processed insect meal, because the presence of non-protein nitrogen in insects leads to an overestimation of the insect protein content. The aim of this paper was, on the basis of our previous study, to recalculate the digestibility of partially or totally defatted black soldier fly (BSF; Hermetia illucens) larva meals in broiler chickens. Sixty 26-days-old Ross 308 male chickens were randomly divided into fifteen cages (5 replicates/treatment). The chickens were fed a basal diet, and two BSF larva meal diets that differed according to their fat content. The digestibility trial lasted 10 days, and it included a 6-days adaptation period and 4-days of excreta collection. Four methods were used to recalculate the crude protein (CP) content of the diet and excreta, using either the conventional 6.25 N:P conversion ratio, the mean 5.60 N:P conversion ratio proposed in literature, or the weighted average of the specific N:P conversion ratio (5.62 for BSF meal, 5.68 for corn and 5.64 for soybean meal). The data were analysed using the t-test and differences were considered significant for P<0.05. Diet CP digestibility calculated using the conventional 6.25 N:P conversion factor was slightly lower than that calculated using the weighted average 5.66 and 5.65 N:P conversion factors (P<0.005). A significantly lower diet CP digestibility was found when using the conventional 6.25 N:P conversion factors for the basal diet and excreta and the weighted average 6.09 N:P conversion factors for the insect meal diets (P<0.005). As far as the latest developments pertaining the N:P conversion ratio in processed insect meal are concerned, the mean 5.60 N:P conversion ratio seems more accurate than the conventional 6.25 one to determine the CP content in poultry diets containing insect processed meal.

scholarly journals Dietary hybrid phytase and carbohydrases on nutrient digestibility and bone quality of broiler chickens

2021 ◽  
Vol 56 ◽  
Author(s):  
Caroline Bavaresco ◽  
Everton Luis Krabbe ◽  
Diego Surek ◽  
Edenilse Gopinger ◽  
Fernando Nicolas Martinez ◽  
...  
Keyword(s):  
Bone Quality ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Positive Control ◽  
Nutrient Digestibility ◽  
Metabolizable Energy ◽  
Available P ◽  
Available Phosphorus ◽  
Poultry Diets

Abstract The objective of this work was to evaluate the effect of the use of hybrid phytase, alone or combined with carbohydrases, in poultry diets with nutritional reductions of calcium, available phosphorus, and metabolizable energy on the nutrient digestibility and bone quality of broiler chickens. A total of 1,875 broilers were distributed in five treatments in a completely randomized design, with 15 replicates of 25 chickens each. The treatments consisted of a positive control feed (T1) and of four negative controls (T2 to T5): T1, basal diet (BD) with corn and soybean; T2 and T3, BDs with reductions of 70 and 100 kcal kg-1 metabolizable energy, respectively, and both with reductions of 0.16% Ca and 0.15% available P; and T4 and T5, BDs with the same nutritional reductions, but supplemented with enzymes, i.e., T4 = T2 + 500 phytase units (FTU) per kilogram and T5 = T3 + 500 FTU kg-1 + 560 xylanase units (TXU) per kilogram + 250 glucanase units (TGU) per kilogram. The use of 500 FTU kg-1 hybrid phytase in pelleted corn-soybean meal diets allows a good digestive performance by broilers and replaces the nitrogen-corrected apparent metabolizable energy at 70 kcal kg-1, as well as 0.16% Ca and 0.15% available P.

Effects of dietary calcium:available phosphorus ratio on calcium tolerance of broiler chickens

1990 ◽  
Vol 30 (4) ◽  
pp. 483 ◽  
Author(s):  
TM Shafey ◽  
MW McDonald
Keyword(s):  
Broiler Chickens ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Available Phosphorus ◽  
Feed Conversion ◽  
3 Dimensional ◽  
The Third ◽  
Regression Relationship ◽  
Third Dimension ◽  
Tibia Ash

The effects of dietary levels of available phosphorus (AP) on calcium (Ca) tolerance of growing chickens were investigated in 3 experiments. In the first experiment, increasing dietary Ca to 21.2 g/kg with AP at 5 g/kg significantly reduced tibia P, plasma P and increased plasma total Ca concentration without any significant effect on performance. With dietary Ca at 25.7 g/kg and AP at 5 g/kg, growth and plasma P were reduced and plasma total Ca was increased, whilst Ca at 30 g/kg diet reduced growth, plasma P, tibia P and increased feed conversion ratio (FCR) and plasma total Ca. Increasing dietary AP to 10 g/kg diet with Ca level at 10.6 g/kg reduced growth and increased FCR without affecting any of the other parameters. Experiment 2 was a 3-dimensional composite design with a central basal diet having Ca and AP concentrations of 12.7 and 4.6 g/kg, The first dimension was 4 dietary concentrations of Ca from 15 to 30 g/kg; the second dimension was 4 dietary concentrations of AP from 6.3 to 12.4 g/kg; and the third dimension was 4 dietary Ca : AP combinations spanning the above ranges but with the Ca: AP ratio maintained at approximately 2.5 (i.e. 15.8 and 6.3; 20.5 and 8.2; 25.5 and 10.2; 30 and 12.1 g/kg for Ca and AP, respectively). A dietary Ca concentration of 25.4 g/kg increased FCR, whilst 30 g Ca/kg reduced growth and increased FCR. Dietary AP of 12.4 g/kg reduced growth and increased FCR. Growth and FCR were less affected when dietary Ca:AP ratio was kept constant at approximately 2.5 than when the ratio was altered by increasing either mineral alone. This relationship was used to develop a linear regression relationship between chicken growth and dietary Ca: AP ratio. In experiment 3, the effects of excessive dietary levels of Ca (12.7, 25.5 and 33.1 g/kg) and AP (9 and 13 g/kg) were investigated in sexed chickens in a factorial design. Ca at 33.1 g/kg significantly reduced growth, tibia P and plasma P, whilst FCR, plasma total Ca and excreta moisture were significantly increased. Dietary Ca at 25.5 g/kg reduced tibia P and increased excreta Ca. High dietary AP significantly reduced growth and tibia Ca and increased tibia ash, excreta moisture and excreta Ca. Significant interactions between dietary levels of Ca and AP were found for growth, tibia ash, excreta moisture and excreta Ca, and for tibia P and plasma total Ca. Significant interactions between sex and either dietary Ca or AP for tibia ash were found. A Ca x sex interaction was found for tibia P, excreta moisture and excreta P. It was concluded that high dietary levels of Ca (up to 21.2 g/kg) can be tolerated by growing chickens without any significant effect on performance, providing the corresponding level of AP is also high.

scholarly journals The Laetiporus sulphureus Fermented Product Enhances the Antioxidant Status, Intestinal Tight Junction, and Morphology of Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 149
Author(s):  
Wei Chih Lin ◽  
Tzu Tai Lee
Keyword(s):  
Mrna Expression ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Intestinal Morphology ◽  
Feed Conversion ◽  
Control Groups ◽  
Fermented Product ◽  
And Control

The Laetiporus sp. is a fungal species that is traditionally used for medicinal purposes. This study investigated the effects of the Laetiporus sulphureus fermented product (FL) as a feed supplementation on the antioxidant activities, the intestinal Tight Junction (TJ) mRNA expression, and the intestinal morphology of broiler chickens. Four-hundred one-day-old male broilers (Ross 308) were randomly allocated to five experimental diets: (1) a corn-soybean meal basal diet (control), (2) a basal diet replaced with 5% Wheat Bran (5% WB), (3) a basal diet replaced with 10% WB (10% WB), (4) a basal diet replaced with 5% FL (5% FL), and (5) a basal diet replaced with 10% FL (10% FL). The FL-supplemented groups exhibited a better feed conversion ratio in the overall experimental period compared to the WB and control groups. The serum antioxidant profiles of 35-day-old broilers showed that, compared to the control and 10% WB groups, the 5% FL supplementation group had a significantly increased superoxide dismutase activity, while it down-regulated the concentration of malondialdehyde in the serum (p < 0.05). The assessment of selected antioxidant gene expression showed that the 5% FL group significantly elevated heme oxygenase-1 and nuclear factor erythroid 2–related factor 2 expression, compared to the control and WB groups (p < 0.05). Furthermore, both of the FL supplemented groups had a significantly higher expression of glutathione peroxidase and catalase, compared to that of the WB and control groups in the jejunum (p < 0.05). The TJ mRNA expression in the jejunum showed that 5% FL significantly elevated the zonula occludens-1, claudin-1, and mucin-2 expression (p < 0.05), while 5% and 10% FL supplementation significantly improved OCLN expression in both the jejunum and ileum, compared to control group (p < 0.05). The intestinal morphology of 35-day-old broilers showed that a 5% FL supplementation significantly increased the villus height in the ileum and jejunum, compared to the WB and control groups (p < 0.05). Moreover, the 5% and 10% FL supplementation groups had a significantly higher villi:crypt ratio in the ileum, compared to the WB and control groups (p < 0.05). To conclude, FL supplementation improved the antioxidative status, the TJ mRNA expression, and the intestinal morphology, and it was accompanied by a lowered feed conversion ratio in broilers. Finally, 5% supplementation had the overall best results in improving the antioxidant status, TJ mRNA expression, and intestinal morphology of broilers.

Influence of Lactobacillus casei WB 315 and crude fish oil (CFO) on growth performance, EPA, DHA, HDL, LDL, cholesterol of meat broiler chickens

2020 ◽  
Author(s):  
Andreas Berny Yulianto ◽  
Widya Paramita Lokapirnasari ◽  
Rifqi Najwan ◽  
Hana Cipka Pramuda Wardhani ◽  
Nabil Fariz Noor Rahman ◽  
...  
Keyword(s):  
Fish Oil ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Lactobacillus Casei ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Consumption ◽  
Feed Conversion

Background and Objectives: Use of antibiotics as growth promoters in animal feeds has been restricted due to the residues in poultry products such as egg and meat, furthermore to the antibiotic resistant of pathogenic bacteria. The prohibition of their use opens the opportunity for the use of non-antibiotic feed additives such as probiotics. The objectives of this study were to investigate the effect of the addition of  Lactobacillus casei WB 315 and crude fish oil (CFO) to diets on growth performance, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), low density lipoproteins (LDL), high density lipoprotein (HDL), and cholesterol levesl of  broiler chickens. Materials and Methods: In this research, one-day old male broiler chicks were used and divided equally into four groups, namely a basal diet without L. casei WB 315 and without CFO (P0), basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and without CFO (P1), basal diet supplemented without L. casei WB 315 and 1% CFO of total broiler basal feed (P2), and basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed (P3) for 35 days. Results: The results of addition 0.5% Lactobacillus casei WB 315 (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed after 35 days showed significant difference among treatment in feed efficiency (p<0.05), feed conversion ratio (p<0.05), feed consumption (p<0.05), EPA (p<0.05), DHA (p<0.05), increase HDL (p<0.05), reduced the LDL (p<0.05), and reduce cholesterol (p<0.05) in meat broiler chicken. Conclusion: It is concluded that the addition of L. casei WB 315 and crude fish oil (CFO) could significant improve the growth performance (feed efficiency, feed conversion ratio, feed consumption) and could significantly improve EPA, DHA and increase HDL and decrease LDL in meat poultry product.  

scholarly journals The effect of humic acid on zinc accumulation in chicken broiler tissues

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 121-127 ◽  
Author(s):  
I. Herzig ◽  
M. Navrátilová ◽  
J. Totušek ◽  
P. Suchý ◽  
V. Večerek ◽  
...  
Keyword(s):  
Humic Acid ◽  
Blood Serum ◽  
Broiler Chickens ◽  
Day Treatment ◽  
Acid Group ◽  
Adaptation Period ◽  
Control Groups ◽  
Leg Muscles ◽  
The Mean ◽  
And Control

Thirty-six selected male chickens were allocated into four groups C, HA, ZN, ZN + HA, and the experiment was initiated after 4 days of an adaptation period. Group C was fed a complete feed mixture without supplements. Group HA was fed the same diet with 500 mg of humic acid per chicken and day. Group ZN was loaded with 240 mg Zn (as 600 mg ZnSO<sub>4</sub>) per chicken and day, and birds of the group ZN + HA were loaded as those of the latter group, with additional 500 mg of humic acid per chicken and day. The treatments were carried out for 10 days. Subsequently, the chickens were slaughtered and samples of liver, kidneys, leg muscles and blood were collected for Zn level assessment. The Zn levels detected in group C can be considered as the background value, found commonly in broiler chickens fed the complete feed mixtures enriched with trace elements. The following concentrations were detected (mg Zn/kg): 40.1 ± 13.4 in muscles, 81.0 ± 6.6 in kidneys, 72.2 ± 15.0 in liver, and in blood serum 1.04 ± 0.45 mg Zn/l. Increased Zn levels by 11 to 30% (<i>P</i> > 0.05) compared to group C were found in group HA in all tissues studied. The ten-day treatment with 600 mg ZnSO<sub>4</sub> (240 mg Zn/day, i.e. 2 400 mg Zn per 10 days) resulted in increased Zn levels in all investigated tissues, significantly in kidneys (<i>P</i> < 0.01), liver (<i>P</i> < 0.01) and blood serum (<i>P</i> < 0.01). The mean levels 430.5 ± 159.0, 149.8 ± 41.9 and 57.9 ± 22.7 mg Zn/kg, and 4.14 ± 0.9 mg/l were found in liver, kidneys, leg muscles, and blood serum, respectively. After the same treatment with zinc sulphate together with humic acid (group ZN + HA), no significant changes of Zn levels in the investigated tissues were reported. Concentrations of the selected parameters of metabolism in chickens ranged within the reference limits; significant differences between experimental and control groups (cholesterol <i>P</i> < 0.05; lactate <i>P</i> < 0.05; calcium <i>P</i> < 0.01) were found sporadically.

scholarly journals Image Enhanced Mask R-CNN: A Deep Learning Pipeline with New Evaluation Measures for Wind Turbine Blade Defect Detection and Classification

2021 ◽  
Vol 7 (3) ◽  
pp. 46
Author(s):  
Jiajun Zhang ◽  
Georgina Cosma ◽  
Jason Watkins
Keyword(s):  
Deep Learning ◽  
Wind Turbine ◽  
Turbine Blade ◽  
Defect Detection ◽  
Recognition Rate ◽  
Weighted Average ◽  
Wind Turbine Blade ◽  
Evaluation Measures ◽  
Augmentation Techniques ◽  
The Mean

Demand for wind power has grown, and this has increased wind turbine blade (WTB) inspections and defect repairs. This paper empirically investigates the performance of state-of-the-art deep learning algorithms, namely, YOLOv3, YOLOv4, and Mask R-CNN for detecting and classifying defects by type. The paper proposes new performance evaluation measures suitable for defect detection tasks, and these are: Prediction Box Accuracy, Recognition Rate, and False Label Rate. Experiments were carried out using a dataset, provided by the industrial partner, that contains images from WTB inspections. Three variations of the dataset were constructed using different image augmentation settings. Results of the experiments revealed that on average, across all proposed evaluation measures, Mask R-CNN outperformed all other algorithms when transformation-based augmentations (i.e., rotation and flipping) were applied. In particular, when using the best dataset, the mean Weighted Average (mWA) values (i.e., mWA is the average of the proposed measures) achieved were: Mask R-CNN: 86.74%, YOLOv3: 70.08%, and YOLOv4: 78.28%. The paper also proposes a new defect detection pipeline, called Image Enhanced Mask R-CNN (IE Mask R-CNN), that includes the best combination of image enhancement and augmentation techniques for pre-processing the dataset, and a Mask R-CNN model tuned for the task of WTB defect detection and classification.

scholarly journals Influence of Enzyme Supplementation in the Diets of Broiler Chickens Formulated with Different Corn Hybrids Dried at Various Temperatures

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 643
Author(s):  
Franciele C. N. Giacobbo ◽  
Cinthia Eyng ◽  
Ricardo V. Nunes ◽  
Cleison de Souza ◽  
Levy V. Teixeira ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Nutrient Digestibility ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Intestinal Morphology ◽  
Feed Conversion ◽  
Corn Hybrids ◽  
Positive Effects ◽  
Cecal Microbiota ◽  
Enzyme Supplementation

We evaluated the influence of enzymatic supplementation on the growth performance and cecal microbiota of broilers. A total of 2160 1-day-old male chicks were used in a 3 × 2 × 2 factorial arrangement (three corn hybrids, two drying temperatures −80 and 110 °C, with or without the inclusion of an enzymatic blend (amylase, xylanase, and protease) (20 birds/pen, n = 9). For all performance and digestibility parameters, we observed, in general, isolated effects of the corn hybrids and drying temperature. Birds that received the enzymatic blend in the diet showed better weight gain from 1 to 21 days (d) and better digestibility coefficients of nutrients at 42 d. Birds fed diets with corn dried at 80 °C showed a better feed conversion ratio from 1 to 42 d. At 21 d of age, enzymatic supplementation had positive effects on jejunum morphology. Enzyme supplementation increased the abundance of the phylum Tenericutes, class Bacilli and Mollicutes, reduced Clostridia, and increased the abundances of the families Lactobacillaceae, Anaeroplasmataceae, and O_RF39;F. In conclusion, the addition of amylase, xylanase, and protease led to a better nutrient digestibility, performance, and intestinal morphology. In addition, enzyme supplementation changed the diversity, composition, and predicted function of the cecal microbiota at d 21.

scholarly journals Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Serum Globulin ◽  
Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

scholarly journals Effects of Clostridium butyricum- and Bacillus spp.-Based Potential Probiotics on the Growth Performance, Intestinal Morphology, Immune Responses, and Caecal Microbiota in Broilers

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 624
Author(s):  
Xinfu Zeng ◽  
Qing Li ◽  
Caimei Yang ◽  
Yang Yu ◽  
Zixian Fu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Short Chain Fatty Acids ◽  
Clostridium Butyricum ◽  
Intestinal Morphology ◽  
Treatment Groups ◽  
Body Weights ◽  
Bacillus Spp

We aimed to investigate the effects of Clostridium butyricum-, Bacillus subtilis-, and Bacillus licheniformis-based potential probiotics on the growth performance, intestinal morphology, immune responses, and caecal short chain fatty acids (SCFAs) and microbial structure in broiler chickens. Three treatment groups containing a total of 1200 one-day-old AA broilers were included: birds fed with a basal diet only (Con), birds fed with added 1010 probiotics cfu/kg (ProL), and birds fed with added 1011 probiotics cfu/kg (ProH). The dietary probiotics significantly improved the final and average body weights and serum immunoglobulins A, M, and Y. The probiotics also enhanced the ileal morphology and improved the caecal acetate, butyrate, and propionate contents. Furthermore, 16S rRNA sequencing revealed that dietary compound probiotics modulated the caecal microflora composition as follows: (1) all birds shared 2794 observed taxonomic units; (2) treatment groups were well separated in the PCA and PCoA analysis; (3) the relative abundance of Parabacteroides, Ruminococcaceae_UCG-014, Barnesiella, Odoribacter, [Eubacterium_coprostanoligenes_group], [Ruminococcus]_torques_group, and Butyricimonas significantly varied between treatments. The compound probiotics improved the growth performance, serum immune responses, the ratio of ileal villus height to crypt depth, and major caecal SCFAs in broiler chickens. The dietary C. butyricum-, B. subtilis-, and B. licheniformis-based probiotics improved overall broiler health and would benefit the poultry industry.

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