scholarly journals The Laetiporus sulphureus Fermented Product Enhances the Antioxidant Status, Intestinal Tight Junction, and Morphology of Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 149
Author(s):  
Wei Chih Lin ◽  
Tzu Tai Lee

The Laetiporus sp. is a fungal species that is traditionally used for medicinal purposes. This study investigated the effects of the Laetiporus sulphureus fermented product (FL) as a feed supplementation on the antioxidant activities, the intestinal Tight Junction (TJ) mRNA expression, and the intestinal morphology of broiler chickens. Four-hundred one-day-old male broilers (Ross 308) were randomly allocated to five experimental diets: (1) a corn-soybean meal basal diet (control), (2) a basal diet replaced with 5% Wheat Bran (5% WB), (3) a basal diet replaced with 10% WB (10% WB), (4) a basal diet replaced with 5% FL (5% FL), and (5) a basal diet replaced with 10% FL (10% FL). The FL-supplemented groups exhibited a better feed conversion ratio in the overall experimental period compared to the WB and control groups. The serum antioxidant profiles of 35-day-old broilers showed that, compared to the control and 10% WB groups, the 5% FL supplementation group had a significantly increased superoxide dismutase activity, while it down-regulated the concentration of malondialdehyde in the serum (p < 0.05). The assessment of selected antioxidant gene expression showed that the 5% FL group significantly elevated heme oxygenase-1 and nuclear factor erythroid 2–related factor 2 expression, compared to the control and WB groups (p < 0.05). Furthermore, both of the FL supplemented groups had a significantly higher expression of glutathione peroxidase and catalase, compared to that of the WB and control groups in the jejunum (p < 0.05). The TJ mRNA expression in the jejunum showed that 5% FL significantly elevated the zonula occludens-1, claudin-1, and mucin-2 expression (p < 0.05), while 5% and 10% FL supplementation significantly improved OCLN expression in both the jejunum and ileum, compared to control group (p < 0.05). The intestinal morphology of 35-day-old broilers showed that a 5% FL supplementation significantly increased the villus height in the ileum and jejunum, compared to the WB and control groups (p < 0.05). Moreover, the 5% and 10% FL supplementation groups had a significantly higher villi:crypt ratio in the ileum, compared to the WB and control groups (p < 0.05). To conclude, FL supplementation improved the antioxidative status, the TJ mRNA expression, and the intestinal morphology, and it was accompanied by a lowered feed conversion ratio in broilers. Finally, 5% supplementation had the overall best results in improving the antioxidant status, TJ mRNA expression, and intestinal morphology of broilers.

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 643
Author(s):  
Franciele C. N. Giacobbo ◽  
Cinthia Eyng ◽  
Ricardo V. Nunes ◽  
Cleison de Souza ◽  
Levy V. Teixeira ◽  
...  

We evaluated the influence of enzymatic supplementation on the growth performance and cecal microbiota of broilers. A total of 2160 1-day-old male chicks were used in a 3 × 2 × 2 factorial arrangement (three corn hybrids, two drying temperatures −80 and 110 °C, with or without the inclusion of an enzymatic blend (amylase, xylanase, and protease) (20 birds/pen, n = 9). For all performance and digestibility parameters, we observed, in general, isolated effects of the corn hybrids and drying temperature. Birds that received the enzymatic blend in the diet showed better weight gain from 1 to 21 days (d) and better digestibility coefficients of nutrients at 42 d. Birds fed diets with corn dried at 80 °C showed a better feed conversion ratio from 1 to 42 d. At 21 d of age, enzymatic supplementation had positive effects on jejunum morphology. Enzyme supplementation increased the abundance of the phylum Tenericutes, class Bacilli and Mollicutes, reduced Clostridia, and increased the abundances of the families Lactobacillaceae, Anaeroplasmataceae, and O_RF39;F. In conclusion, the addition of amylase, xylanase, and protease led to a better nutrient digestibility, performance, and intestinal morphology. In addition, enzyme supplementation changed the diversity, composition, and predicted function of the cecal microbiota at d 21.


2012 ◽  
Vol 52 (9) ◽  
pp. 874 ◽  
Author(s):  
Alex Tsungyu Hung ◽  
Shu-Yuan Lin ◽  
Tsung-Yu Yang ◽  
Chun-Kuang Chou ◽  
Hsun-Cheng Liu ◽  
...  

An experiment was conducted to evaluate the effects of Bacillus coagulans ATCC 7050 (B. coagulans) on the performance, intestinal morphology and microflora of broiler chickens. Six-hundred 1-day-old broilers were randomly assigned into five treatment groups with four replicates for 42 days. There were five dietary treatments: negative control, positive control (antibiotics, 200 mg/kg zinc–bacitracin), 0.10, 0.20 and 0.25 g/kg B. coagulans. Dietary B. coagulans improved feed conversion ratio throughout the entire experimental period compared with the negative control (P = 0.02). Dietary B. coagulans significantly increased the number of lactobacilli (P = 0.01) and tended to decrease coliform bacteria (P = 0.06) in the duodenum. Dietary B. coagulans tended to increase villous height (P = 0.08) but had no effect on crypt depth in the jejunum. There were no significant differences (P > 0.05) in serum cholesterol, triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol between groups. In conclusion, the present data indicate that administration of B. coagulans ATCC 7050 improved feed conversion ratio in broiler chickens, possibly via improving the balance of intestinal microflora.


Author(s):  
SM Amanullah ◽  
R Bateen ◽  
KS Huque ◽  
MR Alam ◽  
MS Alam ◽  
...  

A feeding trial was conducted on 21 crossbred (Local×Friesian, Local×Sahiwal, Sahiwal×Friesian) calves of around one week age for a period of 70 days with the objective of evaluating effects of probiotic on growth performances of calves. Calves were selected and distributed equally into three groups maintaining equal sex ratio in each group, namely the farm practice (FP), control and probiotic groups. The calves were fed on milk as 12% of their live weight up to 14 days and then 10% milk of their live weight up to 70 days of age and wheat bran was supplied to the calves from 50th day at the rate of 250g/calf. The calves of probiotic group werefedaprobiotic mixture containing lactic acid bacteria (LAB) at 0.5 g /d. The data of feed intake and growth for all groups were recorded up to 35 days and then up to 70 days for probiotic and control groups. The calves under probiotic group were achieved 82.8% and 74.5% higher gain (P<0.05) than that of FP and control group, respectively and feed conversion ratio was observed significantly (P<0.05) better in probiotic group (1.83) than that in FP (3.41) and the control group (3.50) up to 35 days of trial. However, no significant differences were found in average live weight gain and feed conversion ratio between probiotic and the control groups (1.99 vs 2.07, respectively) up to 70 days of the trial. Average counts of E. coli were lower in probiotic group than that in the control and FP. The incidence of diarrhoea in probiotic fed group was found 3 and 13 times lower compared to FP and the control group, respectively. Bangladesh J. of Livestock Res. 21-25: 89-102, 2018


2020 ◽  
Vol 42 (1) ◽  
Author(s):  
Hoang Nghia Son

This paper examined the effects of nano mineral supplementation of diets on the growth of pigs. The total of 180 pigs used for this study were classified into 4 experimental groups and one control group. The results showed that the weight of pigs at the beginning and the end of the experiment was insignificantly different between experimantal and control groups. The same daily gain weight was observed between experimental and control groups. However, the feed conversion ratio (FCR) of four experimental groups I, II, III and IV is 3.00 ± 0.28, 2.95 ± 0.11, 2.85 ± 0.19 and 2.90 ± 0.24, respectively, that were lower than of the control groups, i.e. 3.17 ± 0.42. The mineral content of Fe, Cu, Zn and Mn in liver of pigs in all the experimental groups is lower than that in liver of pigs in the control group. In addition, the amount of Fe and Zn metal in urine and feces of the experimental groups is also lower than in the control group. The results of our study showed that the addition of nano minerals in diets for pigs not only reduced the feed conversion ratio in pigs, also decreased the amount of minerals releasing into the environment.  


Author(s):  
Andreas Berny Yulianto ◽  
Widya Paramita Lokapirnasari ◽  
Rifqi Najwan ◽  
Hana Cipka Pramuda Wardhani ◽  
Nabil Fariz Noor Rahman ◽  
...  

Background and Objectives: Use of antibiotics as growth promoters in animal feeds has been restricted due to the residues in poultry products such as egg and meat, furthermore to the antibiotic resistant of pathogenic bacteria. The prohibition of their use opens the opportunity for the use of non-antibiotic feed additives such as probiotics. The objectives of this study were to investigate the effect of the addition of  Lactobacillus casei WB 315 and crude fish oil (CFO) to diets on growth performance, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), low density lipoproteins (LDL), high density lipoprotein (HDL), and cholesterol levesl of  broiler chickens. Materials and Methods: In this research, one-day old male broiler chicks were used and divided equally into four groups, namely a basal diet without L. casei WB 315 and without CFO (P0), basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and without CFO (P1), basal diet supplemented without L. casei WB 315 and 1% CFO of total broiler basal feed (P2), and basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed (P3) for 35 days. Results: The results of addition 0.5% Lactobacillus casei WB 315 (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed after 35 days showed significant difference among treatment in feed efficiency (p<0.05), feed conversion ratio (p<0.05), feed consumption (p<0.05), EPA (p<0.05), DHA (p<0.05), increase HDL (p<0.05), reduced the LDL (p<0.05), and reduce cholesterol (p<0.05) in meat broiler chicken. Conclusion: It is concluded that the addition of L. casei WB 315 and crude fish oil (CFO) could significant improve the growth performance (feed efficiency, feed conversion ratio, feed consumption) and could significantly improve EPA, DHA and increase HDL and decrease LDL in meat poultry product.  


ZOOTEC ◽  
2016 ◽  
Vol 37 (1) ◽  
pp. 8
Author(s):  
Claudia Mangelep ◽  
F. R. Wolayan ◽  
M. R. Imbar ◽  
I. M. Untu

THE EFFECT OF PARTLY SUBSTITUTION OF DIET WITH WHITE CHINESE CABBAGE (Brasscia pekinensia L) WASTE PRODUCT ON BIOLOGICAL PERFORMANCE OF BROILER CHICKEN The present study was conducted to characterize the effect of white Chinese cabbage (Brasscia pekinensia L) waste product as an alternative feed source for broiler chicken.  The experiment was conducted at Faculty of Animal Husbandry’s experimetal station for 21 days using 60 MB-202 broiler chicken. The experiment was started at 21 days of age and terminated at 42 days of age. Birds were allocated to 20 experimental battery cage, 3 birds in each cage. Completely Randomized Design (CRD) with 5 treatments and 4 replications was employed to analyze experimental data. Treatment diets were formulated as follow: R0 = 100% basal diet and 0% white Chinese cabbage waste product; R1 = 95% basal diet and 5% white Chinese cabbage waste product; R2 = 90% basal diet and 10% white Chinese cabbage waste product; R3 = 85% basal diet and 15% white Chinese cabbage waste product; and R4 = 80% basal diet and 20% white Chinese cabbage waste product. Parameters measured were: 1) daily feed consumption; 2) daily gain; and 3) feed conversion ratio (FCR). The results of the study during which feed consumption (138,09-141,57g), weight gain (64,95-68,53g), and feed conversion (2,07-2,12). The results showed that partly substitution (up to 20%) of basal diet with white Chinese cabbage waste product gave non significant (P > 0.05) effect on feed consumption, daily gain, and feed conversion ratio (FCR) of broiler chicken. It can be concluded that white Chinese cabbage waste product can be used up to 20% feed gives good results on broiler performance. Key Words: Substitution, white Chinese cabbage waste product, broiler performance


2008 ◽  
Vol 88 (4) ◽  
pp. 613-622 ◽  
Author(s):  
O. Zimonja ◽  
H. Hetland ◽  
N. Lazarevic ◽  
D. H. Edvardsen ◽  
B. Svihus

Two experiments were conducted to study the effects of either soluble or insoluble fibre naturally occurring in wheat and oats, on technical pellet quality and nutritional value for broiler chickens. In exp. 1, the effects of insoluble fibre were studied by mixing diets based on wheat or naked oats with either coarse or fine oats hulls followed by pelleting. Hull inclusion increased (P < 0.05) starch gelatinisation, especially in the diets based on oats. Addition of finely ground oats hulls improved pellet durability, in particular after storage, while no such effect was observed for coarse oats hulls. Oats hulls inclusion caused a more elastic pellet with higher breaking resistance (P < 0.05). Lower (P < 0.05) energy consumption was observed for diets based on oats compared with wheat, while no effect of fibre inclusion on energy consumption was found. In exp. 2, the influence of pelleting on the viscosity effect of soluble fibres in diets based on wheat or oats was studied. A significant increase (P < 0.05) in intestinal viscosity of broiler chickens was observed for all batches of grain exposed to steam pelleting. Viscosity was doubled (P < 0.05) with wheat diets, while the increase in viscosity was even greater in oat diets. In wheat diets, feed intake and weight gain was reduced (P < 0.05), while feed conversion ratio was not affected. Steam pelleting of oats reduced (P < 0.05) feed intake and weight gain, and increased feed conversion ratio (P < 0.05). The improvements (P < 0.05) in metabolisable energy and dry matter digestibility in wheat diets was not consistent with the observed increases in viscosity or with the results obtained with oats. Key words: Soluble fibres, insoluble fibres, viscosity, starch gelatinisation, steam-pelleting


2020 ◽  
Vol 47 (4) ◽  
pp. 132-138
Author(s):  
E. Opoola

The present study was conducted to determine the effect of silicon oxide in broiler chickens drinking water on performance and litter quality. A total of 120, one-day old mixed sex broiler chicks were randomly divided into 4 treatments and 3 replications, with 10 birds in each replicate in a completely randomised experimental design, with 4 silicon levels (0.00, 1.50, 2.00 and 2.50 mg of silicon oxide/litre of water. Corn-soya based diets were formulated at the starter (0–4 weeks) and finisher (5–8weeks) phases and were supplied ad libitum with free access to water. The average daily temperature and relative humidity inside the poultry house were 35.9 °C and 36%, respectively. At the starter phase, chicks administered 2.00mg silicon oxide/litre of water had significantly (p<0.05) higher final body weight (991.00g) and average weight gain (950.96g) compared to chicks without silicon oxide 778.57 g and 738.54g. The inclusion of 2.00 mg silicon oxide/litre of water improved feed conversion ratio compared to the control group (p < 0.05) and significantly reduced feed cost per kg gain. For the finisher phase, chickens administered 2.00mg silicon oxide/litre of water had the best results for final weight, weight gain and feed cost per kg gain. Also, inclusion of silicon oxide in broiler chickens water improved litter quality as predominantly dry material but with some areas of wet shavings was observed. In conclusion, silicon oxide in broiler chickens drinking water at 2.00mg/litre of water enhanced growth performance, improved feed conversion ratio and litter quality as well as reduced feed cost per kg gain at the starter and finisher phases respectively.


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