Efficacy of Mozambican bentonite and diatomaceous earth in reducing the toxic effects of aflatoxins in chicks

2016 ◽  
Vol 9 (1) ◽  
pp. 63-72 ◽  
Author(s):  
F.R. Dos Anjos ◽  
D.R. Ledoux ◽  
G.E. Rottinghaus ◽  
M. Chimonyo

The effectiveness of diatomaceous earth (DE) and bentonite clay (BC) in reducing the toxic effects of aflatoxin B1 (AFB1) in chicks was assessed. A total of 150 day-old male broiler chicks were assigned to six dietary treatments, with 5 replicate pens of five chicks per treatment. Dietary treatments were: (1) a positive control basal diet (BD) containing no AFB1, no BC and no DE; (2) BD + 0.50% BC but with no AFB1 and DE; (3) BD + 0.50% DE but with no AF and BC; (4) BD + 2 mg AFB1/kg diet but with no BC and DE; (5) BD + 0.50% BC + 2 mg AFB1/kg diet but with no DE; and (6) BD + 0.50% DE + 2 mg AFB1/kg diet but with no BC. Compared with controls, feed intake (FI) and body weight gain (BWG) were depressed (P<0.05) in chicks fed AFB1, with greater reduction in FI and BWG observed in birds fed the AFB1 plus 0.50% DE diet. Feed conversion ratio was similar. Chicks fed AFB1 alone had increased (P<0.05) relative liver weights compared to all other dietary treatments except for chicks fed AFB1+ DE. Chicks fed AFB1 alone and those fed AFB1+ DE had heavier (P<0.05) relative kidney weights compared to chicks fed other treatments. Compared with the control chicks, livers from birds fed dietary AFB1 and AFB1 plus DE were similar. Liver lesion score of chicks fed AFB1 plus BC was lower (P<0.05) than that of chicks fed AFB1 but higher (P<0.05) than that of control chicks. Compared to controls, serum concentrations of glucose, albumin, total protein globulin, and calcium were decreased (P<0.05) in chicks fed AFB1 alone, whereas serum concentrations of aspartate amino transferase and gamma glutamyl transferase were increased (P<0.05). It can be concluded that this concentration of BC was partially effective in reducing the toxic effects of 2 mg/kg AFB1, whereas DE at the same concentration was not effective in reducing the toxic effects of 2 mg/kg AFB1.

2020 ◽  
Vol 9 (8) ◽  
pp. e74985242
Author(s):  
Jalceyr Pessoa Figueiredo Junior ◽  
Fernando Guilherme Perazzo Costa ◽  
Ricardo Romão Guerra ◽  
Marcelo Helder Medeiros Santana ◽  
Matheus Ramalho de Lima ◽  
...  

The aim of this study was was to determine the nutritional requirements of digestible methionine+cystine (M+C) for white-egg layers aged one to six weeks. A completely randomized design with five methionine+cystine levels, six replicates, and 30 birds per experimental unit was adopted. Dietary treatments consisted of five diets supplemented with DL-Methionine with resulted in five levels of digestible methionine + cystine, 80% (0.516%), 90% (0.578%), 100% (0.640%), 110% (0.702%), and 120% (0.764%), based on Brazilian tables of nutritional requirements. Performance, serological blood, and histological data were evaluated. Feed intake, feed conversion, hepatic glycogen deposition, enzymatic activity of alanine aminotransferase and gamma-glutamyl transferase, and serum creatinine and albumin levels had showed a quadratic response to the levels of digestible M+C, with the respective requirements: 89.78% (0.575%), 114.33% (0.732%), 86.50% (0.554%), 100% (0.640%), 100.40% (0.643%), 104.30% (0.668%), and 111.88% (0.716%). Increasing levels of methionine+cystine elevated the relative liver weight and the deposition of hepatic glycogen, in addition to promote higher growth in pullets, with better body weight and body weight gain and feed conversion ratio. Our findings suggest that 0.732% digestible methionine+cystine is recommended, which corresponds to an intake of 151.20 mg/bird/d and a Met+Cys:Lys  ratio 83%, for light pullets from one to six weeks.


2020 ◽  
Vol 42 ◽  
pp. e47222
Author(s):  
Jalceyr Pessoa Figueiredo Júnior ◽  
Fernando Guilherme Perazzo Costa ◽  
Ricardo Romão Guerra ◽  
Marcelo Helder Medeiros Santana ◽  
Matheus Ramalho de Lima ◽  
...  

An experiment was carried out to evaluate the requirement of digestible methionine for growing pullets at growth phase (7 to 12 weeks of age). A completely randomized design was distributed in five treatments, six replicates, and 15 pullets per experimental unit. 450 Dekalb White pullets from the 7th weeks of age, with an average initial weight of 313.14 ± 12.49 g were used. Dietary treatments consisted in five diets supplemented with DL-Methionine which resulted in five levels of digestible methionine (0.266, 0.294, 0.322, 0.350, and 0.378 %). Performance, serological blood, histopathology and histomorphometry data were evaluated. Quadratic responses were observed for final live weight (p < 0.0143), weight gain (p < 0.0073), feed conversion ratio (p < 0.0058), glycogen deposition in the liver (p < 0.0001), gamma-glutamyl transferase enzyme activity (p < 0.0008), and villus height (p < 0.0024) with digestible dMet levels. In conclusion, the use of 0.343 % dMet, corresponding to a dMet:dLys ratio 55, is recommended for white-egg pullets from 7 to 12 weeks of age.


2007 ◽  
Vol 55 (4) ◽  
pp. 479-490 ◽  
Author(s):  
N. Senkoylu ◽  
H. Samli ◽  
M. Kanter ◽  
Aylin Agma

Day-old male chicks were used to test the effects of a combination of formic and propionic acids (CFP) added to wheat- and barley-based diets on the performance and gut histomorphology of broilers. The CFP, containing formic and propionic acids based on an inorganic phyllo-silicate carrier, was added to starter and grower broiler diets of standard (NRC, 1994) or lower nutrient density (LND) using different levels of wheat and barley. Body weight gain (BWG) and feed conversion ratio (FCR) of broiler chicks were significantly (P < 0.001) improved by CFP supplementation at 21 days of age regardless of nutrient density (ND). However, at 35 days of age CFP addition significantly (P < 0.001) increased BWG and FCR. Proventriculus weight was also significantly affected by the dietary treatments. Ileal viscosity was significantly (P < 0.05) affected by ND which was associated with wheat and barley contents, whereas feed viscosity was significantly (P < 0.05) affected by CFP supplementation. Villus height was significantly (P < 0.05) increased by the CFP supplementation. In the case of villus width, an interaction effect was observed: there was a significant (P < 0.05) decrease in the group fed the standard ND diet, but only a numerical decrease in the group fed the LND diet compared to control group. No significant effect of dietary treatment on the thickness of the lamina muscularis mucosae could be detected.


2021 ◽  
Vol 104 (4) ◽  
pp. 003685042110619
Author(s):  
Muhammad Aziz ur Rahman ◽  
Usama Jamal ◽  
Urooj Anwar ◽  
Muhammad Qamar Bilal ◽  
Muhammad Riaz ◽  
...  

The purpose of the research was to investigate the effects of including potato peels (PP) in broilers diet with exogenous enzymes supplementation on feed intake (FI), body weight gain (BWG), nutrient digestibility and carcass parameters. For this purpose, five iso-caloric and iso-nitrogenous rations were formulated with different levels of PP (0, 5, 10, 15 and 20%). Experimental diets were supplemented with a blend of exogenous enzymes (Xylanase, Mannanase, Protease, Cellulase). A total of four hundred male broiler chicks (day old) of 38 ± 3 gram were randomly distributed into 5 experimental groups in such a way that each group had 8 replicates and each replicate had 10 birds. Results revealed that the inclusion of PP @ 5% with exogenous enzymes had similar FI, BWG and nutrients digestibility with control. However, the inclusion of P @ of 10, 15, and 20% with exogenous enzymes in the broiler diet adversely affected the BWG, feed conversion ratio and nutrient digestibility ( p < 0.05). Results of carcass parameters were not affected ( p > 0.05) by dietary treatments. Based on findings, it is concluded that 5% addition of PP with exogenous enzymes could be practiced in broiler diet without any adverse effect on the performance of commercial broilers.


Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 416 ◽  
Author(s):  
Dinh Hai Nguyen ◽  
In Ho Kim

We investigated the effects of a blend of organic acids (OAs) and medium-chain fatty acids (MCFAs) supplementation in 800 1-d-old male Ross 308 broiler chickens (42 ± 0.90 g) in a 7-week study. Broiler chicks were randomly allocated into one of the five dietary treatments (16 birds per pen with 10 pens per treatment). Dietary treatments consisted of corn-soybean meal based basal diet and the basal diet supplemented with blend of OAs and MCFAs at 0.25, 0.5, 0.75 g, and 1 g per kg of feed. In the current study, during the whole experimental period, the inclusion of the blend of OAs and MCFAs in the basal diet linearly improved (p < 0.05) body weight gain (BWG), feed conversion ratio (FCR), and dry matter digestibility. The increasing inclusion of the blend of OA and MCFA levels in the diets linearly decreased (p = 0.002) feed intake during d 1 to 7. Broilers fed diets containing different levels of the blend of OAs and MCFAs showed a linear increase (p = 0.006) in Lactobacillus concentrations and decrease (p = 0.014) in ammonia (NH3) at the end of the experiment. However, the blend of OAs and MCFAs did not affect carcass quality, E. coli, and Salmonella counts, as well as hydrogen sulfide and total mercaptans gas emission (p > 0.05). In conclusion, the blend of OA and MCFA supplementation positively influenced growth performance, DM digestibility, excreta Lactobacillus counts, as well as NH3 gas emission in broiler chickens.


2015 ◽  
Vol 8 (2) ◽  
pp. 1600-1606
Author(s):  
Huda Mohammed Saad Elamin ◽  
K.A. Mohamed ◽  
Mukhtar A. Mukhtar

The present study was planned to evaluate the response of broiler chicks to diets containing graded levels of spearmint (Mentha spicata).  A total of 200 , five days-old, unsexes (cob) broiler chicks were used. Chicks were weighed individually and assigned randomly to five groups of 40 chicks per treatment and each treatment was subdivided to four replicates of 10 chicks each. Five experimental diets were formulated (A, B, C, D and E) to meet the nutrient requirements of broiler chicks. The experimental parameters examined were growth performance, serum metabolite, electrolyte and enzyme activities and economic appreciable. Diet (A) Negative      Control (NC), a basal diet without antibiotic or spearmint,  diet B as Positive Control (PC) basal diet supplemented with antibiotic (Neomycin 16 mg/Kg), diets C,D and E were basal diet  supplemented with spearmint at levels of 1,1.5,and 2%, respectively. Result obtained showed that addition of spearmint improved significantly (P<0.05) the performance (body weight gain and feed conversion ratio) of broiler chicks compared to group fed on NC while no significant (P<0.05) differences between chicks fed on diets supplemented with spearmint  and PC group observed, no significantly difference was found in feed consumption between all treated groups. The mortality rate did not influenced by the dietary treatments. Results revealed no significant differences (P<0.05) observed among all treatment groups in the carcass dressing percentages, Supplementation of broiler diets with spearmint at various levels was resulted in significant (p>0.05) reduction of abdominal fat, serum cholesterol, and AST enzyme activity compared to both NC and PC groups while the levels of urea, total protein, glucose and ALP and serum electrolytes levels remained unchanged. The highest profitability ratio (1.13) was obtained from the diet (E) supplemented with 2% spearmint. This study showed that spearmint when added as growth promoter in broiler diets has a similar effect on performance as that of antibiotic without any adverse effects.


2015 ◽  
Vol 44 (1) ◽  
pp. 33-39
Author(s):  
MF Hossain ◽  
M Khairunnesa ◽  
SC Das

An experiment was conducted with 160, day old Cobb 500 broiler chicks to evaluate the effect of supplementation of Grow Power (GP) on the performance of broiler. The dietary treatments were control (D1), Grow Power (D2), Grow Power plus antibiotic (D3) and antibiotic (D4). At 5th week of age, the body weight of birds at different dietary treatments were 938.75, 1105.00, 1136.25, and 985.00g in the diets of D1, D2, D3 and D4, respectively. Body weight gain at 2nd, 3rd and 4th week of age were significantly (P<0.05) higher in GP supplemented group (D2) and GP plus antibiotic group (D3) than that of control (D1) and antibiotic (D4) groups. Feed consumption during the experimental period was 1753.75, 1938.75, 1917.50 and 1774.50g for D1, D2, D3 and D4, respectively. At 2nd and 3rd week of age, feed consumption were significantly (p<0.05) higher in D3 group than the other groups including control. Significantly (P<0.05) better Feed Conversion Ratio (FCR) was found in D3 (2.03) and D2 (2.11) groups compared to D1 (2.31) and D4 (2.28).  At 3rd, 4th and 5th week of age, the Performance Index (%) was significantly higher in D2 than D1 and D4. Meat yield parameter of broilers showed significant (P<0.05) differences in dressing weight, feather weight, shank weight, drumstick weight, neck weight, liver weight and viscera weight. Based on the results obtained from the current study it may be concluded that the inclusion of Grow Power @ 50gm/100kg of mixed feed with or without antibiotic improved the growth of commercial broiler including their FCR and therefore, this product may be considered as an alternative of traditional antibiotic in broiler diet.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23139Bang. J. Anim. Sci. 2014. 44 (1): 33-39 


Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2027
Author(s):  
Doaa Ibrahim ◽  
Tamer Ahmed Ismail ◽  
Eman Khalifa ◽  
Shaimaa A. Abd El-Kader ◽  
Dalia Ibrahim Mohamed ◽  
...  

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.


2021 ◽  
Vol 9 (6) ◽  
pp. 1341
Author(s):  
Sarayu Bhogoju ◽  
Collins N. Khwatenge ◽  
Thyneice Taylor-Bowden ◽  
Gabriel Akerele ◽  
Boniface M. Kimathi ◽  
...  

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.


Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.


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