In vitro Antibacterial and Antifungal Potentials of Petroleum Ether Extract of Moringa oleifera

2012 ◽  
Vol 7 (2) ◽  
pp. 110-113 ◽  
Author(s):  
J. Das ◽  
S.K. Biswas ◽  
A. Chowdhury ◽  
S.R. Sharif ◽  
M.A. Hannan
Author(s):  
Gautam P. Vadnere ◽  
Md. Rageeb Usman ◽  
Santram Lodhi ◽  
Vaishali Patil

Objective: Present study aimed phytochemical evaluation and antimicrobial screening of petroleum ether and ethanol extracts of Santalum album seeds.Methods: Petroleum ether and ethanol extracts were screened for presence of chemical constituents. Petroleum ether extract was investigated detail by using chromatographic and spectroscopic methods. In vitro antimicrobial activity of both extracts were investigated using disc diffusion method on two gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, gram negative Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans.Results: Santalbic acid was identified in petroleum ether extract and content determined by HPTLC was 4.7%w/w. It was seen that petroleum ether extract have MIC value for B. subtilis, P. aeruginosa, E. coli and C. albicans were 78.125 µg/ml, 19.331 µg/ml, 625 µg/ml & 39.062 µg/ml respectively while MBC was 39.062 µg/ml, 4.882 µg/ml, 312.5 µg/ml & 9.765 µg/ml,  respectively. Petroleum ether extract showed MIC and MBC values for S. aureus was similar as 156.25µg/ml. So, the petroleum ether extract showed significant antimicrobial activity against both gram positive, gram negative and fungal strain.Conclusions: The results of present investigations were indicative of possible high potency of petroleum ether extract due to santalbic acid which could serve as chemotherapeutic agent.


2016 ◽  
Vol 19 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Mir Monir Hossain ◽  
Kishor Majumder ◽  
Amjad Hossain Tipu

The seeds of Helianthus annuus have been investigated for the presence of various chemical components and biological activities with special emphasis to the anti-oxidant, antimicrobial, cytotoxic and thrombolytic assay. The results of standard phytochemical analysis revealed the presence of tannins, saponins, flavonoids, carbohydrates, steroids, fixed oils and vitamins. In vitro antioxidant activity of methanolic seed extract of H. annuus was determined by DPPH free radical scavenging assay, which demonstrated very significant antioxidant potential compared to standard antioxidant (ascorbic acid). In case of antimicrobial assay, the methanolic and ethyl acetate extract of H. annuus seeds showed high sensitivity to Salmonella typhi and moderate sensitivity to Pseudomonous aeuregenosa. The petroleum ether extract revealed high sensitivity to P. aeuregenosa. The methanolic extract of H. annuus displayed LC50 1.2 ug/ml and its petroleum ether extract showed LC50 of 1.1 ug/ml, which indicated significant cytotoxicity.Bangladesh Pharmaceutical Journal 19(1): 100-105, 2016


2021 ◽  
Vol 13 (1) ◽  
pp. 195-208
Author(s):  
J. Chetia ◽  
L. R. Saikia ◽  
S. Upadhyaya ◽  
E. Khatiwora ◽  
A. Bawri

Lamiaceae members are commonly used in ethno-medicinal practices of our country. The in vitro antibacterial and antifungal activity of various solvents extracts of different aerial parts of Ocimum americanum L., O. basilicum L. and O. sanctum L. wereassessed on standard bacterial and fungal strains using standard laboratory methods. Extracts from O. americanum have inhibitory activity against B. subtilis, B. cereus and S. aureus.Acetone extracts of O. basilicum and O. sanctum were found to be more potent being capable of exerting significant inhibitory activities against majority of the bacteria investigated. Acetone extract of young inflorescence of O. americanum showed highest antibacterial activity against B. cereus (14±1mm) which was also higher (10±1mm) than the inhibition of standard Clotrimazole (10mcg). Hot petroleum ether extract of mature leaves of O. basilicum showed highest activity against E. coli (16±2mm) which was also higher than the inhibition ofAmpicillin, Streptomycitin, Erythromycin.Petroleum ether extract of young leaves of O. sanctum recorded highest inhibition against P. vulgaris (20±2mm). Presence of tannins, flavonoids, saponins, phenols in all the parts of the plants. Use of different parts instead of using whole plant will help in sustainable management of these medicinal plants.


2021 ◽  
Vol 8 (12) ◽  
pp. 279-283
Author(s):  
M. A. Garga ◽  
M. K. Ibrahim ◽  
A. Mohammad ◽  
H. A. Abdullahi ◽  
M. B. Yusuf ◽  
...  

In vitro anti-malarial activity of petroleum ether extracts of Ficus sycomorus leaves was investigated using Candle Jar method. The extractions were carried out through activity guided fractionation. Petroleum ether extract has the highest activity, at the concentration of 1mg and 0.5mg with percentage growth inhibition of (33.12 and 31.9%) respectively. These results suggest that the leaves of Ficus sycomorus plant have significant antimalarial activity and that the antiplasmodial agents might be as a result of the extract fractionated using the petroleum.


2008 ◽  
Vol 1 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Tarannum Naz ◽  
Ashik Mosaddik ◽  
M. Ekramul Haque

  Antibacterial and antifungal properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Piper chaba (Choi) roots were studied by disc diffusion method and these activities were compared with primary standard drugs Kanamycin and Nystatin, respectively. The extracts were found to exhibit promising antibacterial and antifungal properties against Gram-positive, Gram-negative bacteria and fungi. The extracts were also studied for their cytotoxic activities by brine shrimp lethality bioassay, where gallic acid was used as primary standard. It was observed that the petroleum ether extract was potent cytotoxic with the LC50 value of 0.95 µg/ml against Artemia salina (L). The essential oils of the petroleum ether extract of Piper chaba roots were analysed by GC/MS. It was observed that most of the compounds were sesquiterpenes, some were long chain fatty acids and some were monoterpenes and alkaloids. Seventeen compounds were identified from the GC/MS analysis. Keywords: Piper chaba, antibacterial activity, antifungal activity, cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1446


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