Inter and Intra-specific Variation in SDS-PAGE Electrophoregrams of Total Seed Protein in Wheat, Barley and Their Wild Species

2000 ◽  
Vol 3 (12) ◽  
pp. 2223-2225
Author(s):  
M.Shahid Masood ◽  
Kazutoshi Okuno ◽  
Rashid Anwar
Keyword(s):  
Wild Species ◽  
Seed Protein ◽  
Sds Page ◽  
Specific Variation ◽  
Total Seed

Inter and Intra-Specific Variation in SDS-PAGE of Total Seed Protein in Rice (Oryza sativa L.) Germplasm

2004 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Rehana Asghar ◽  
Rabia Siddique . ◽  
Muhammad Afzal . ◽  
Shamim Akhtar .
Keyword(s):  
Oryza Sativa ◽  
Seed Protein ◽  
Oryza Sativa L ◽  
Sds Page ◽  
Specific Variation ◽  
Total Seed

Taxonomic significance of seed protein profiles in Acacia, with special reference to Acacia emilioana

2003 ◽  
Vol 16 (1) ◽  
pp. 35 ◽  
Author(s):  
Alicia L. Lamarque ◽  
Renée H. Fortunato
Keyword(s):  
Seed Protein ◽  
Seed Proteins ◽  
High Concentration ◽  
Protein Patterns ◽  
Qualitative And Quantitative ◽  
Sds Page ◽  
Electrophoretic Data ◽  
Main Protein ◽  
Protein Components ◽  
Total Seed

Total seed proteins of 10 Acacia species were examined by SDS–PAGE. The protein patterns showed qualitative and quantitative differences among the taxa analysed. The main protein components of most species examined had MW's in the range of 38.5–49.0 × 103. Subgenus Aculeiferum differed from subg. Acacia in the presence of a high concentration of proteins in the range of 20–24.5 × 103. Hierarchical clustering of the 10 taxa was undertaken, based on Jaccard distances calculated from electrophoretic data. The species grouped in two main clusters, representing the two subgenera of Acacia that occur in America, namely subg. Acacia and subg. Aculeiferum. The taxonomic placement of Acacia emilioana, a species with uncertain sectional affinity within subg. Aculeiferum, is discussed.

scholarly journals Seed protein variability in safflower

2002 ◽  
pp. 29-34
Author(s):  
Dragana Obreht ◽  
Ljiljana Vapa ◽  
Sándor Kis ◽  
Mária-Hajos Takács ◽  
Éva-Bányai Stefánovics ◽  
...  
Keyword(s):  
Protein Content ◽  
Seed Protein ◽  
Seed Proteins ◽  
Carthamus Tinctorius ◽  
Seed Protein Content ◽  
Electrophoretic Patterns ◽  
Sds Page ◽  
The Usa ◽  
Molecular Masses ◽  
Total Seed

Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.

scholarly journals Differences in tomato seed protein profiles obtained by SDS-PAGE analysis

2008 ◽  
Vol 53 (1) ◽  
pp. 13-23
Author(s):  
Elizabeta Miskoska-Milevska ◽  
Blagica Dimitrievska ◽  
Koo Poru ◽  
Zoran Popovski
Keyword(s):  
Seed Protein ◽  
Seed Proteins ◽  
Protein Profiles ◽  
Protein Fragment ◽  
Tomato Seed ◽  
Protein Fragments ◽  
Molecular Weights ◽  
Tomato Seeds ◽  
Sds Page ◽  
Total Seed

The protein profiles of tomato seeds from sub-species ( subsp. cultum Brezh., subsp. subspontaneum Brezh. and subsp. spontaneum Brezh.) were analyzed using SDS-PAGE technique. Electrophoreograms and denzitograms of total, soluble and non-soluble proteins of 31 different samples have showed quantitative and qualitative differences. Qualitative differences in electrophoregrams of total seed proteins refer to protein fragments in zone A (114 kDa, 83 kDa and 65 kDa) and protein fragment in zone C (17 kDa). Qualitative differences in electrophoregrams of soluble seed proteins refer to protein fragment in zone A (94 kDa). Qualitative differences in electrophoregrams of nonsoluble seed proteins refer to protein fragments with molecular weights of: 210 kDa, 85 kDa, 67 kDa and 26 kDa.

Proteomics Variation in Nigella Sativa L. (Rananculaceae) Germplasm

2021 ◽  
Vol 50 (2) ◽  
pp. 289-294
Author(s):  
Muhammad Sajjad Iqbal ◽  
Abdul Ghafoor
Keyword(s):  
Euclidean Distance ◽  
Nigella Sativa ◽  
Crop Improvement ◽  
Band Formation ◽  
First Report ◽  
Sds Page ◽  
Genetic Pattern ◽  
Specific Variation ◽  
Improvement Programs ◽  
Single Seeds

Study revealed a first report of proteomics variation in Nigella sativa L. based on analyzing 32 accessions through SDS-PAGE. Three prominent regions along eight subunits were identified. Intra specific variation was observed low whereas the sharpness of bands was high between first and second regions. It was noted that in second region there was no clear evidence of band formation in N. sativa. Prominent and sharp protein peptide bands were recorded in four accessions, namely PK-020561, PK-020609, PK-020620 and PK-020646. Further investigation of single seeds showed almost similar genetic pattern within the single accession. Five clusters were formed on the basis of Euclidean distance. Cluster-I & II contain 1, 1 accession each, likewise Cluster-III and C-IV contain 2, 2 accessions whereas Cluster-V was found diversified as consisted of 26 accessions. Two accessions PK-020878 and PK-020877 were recommended for polymorphism and crop improvement programs. Bangladesh J. Bot. 50(2): 289-294, 2021 (June)

Studies on the Rosaceae II- SDS-PAGE Seed Protein Electrophoresis and its Significance in the Taxonomy of the Family

2003 ◽  
Vol 6 (21) ◽  
pp. 1820-1829
Author(s):  
A.E. Dowidar ◽  
E.A. Kamel ◽  
A.M. Ahamed ◽  
M.H.A. Loutfy ◽  
H.H.L. Hafez
Keyword(s):  
Seed Protein ◽  
Protein Electrophoresis ◽  
Seed Protein Electrophoresis ◽  
Sds Page ◽  
The Family

Relative Sowing Time and Density of Component Crops in a Maize/Cowpea Intercrop System

1987 ◽  
Vol 23 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Francis Ofori ◽  
W. R. Stern
Keyword(s):  
Seed Protein ◽  
Total Yield ◽  
Maize Yield ◽  
Protein Yield ◽  
Sowing Time ◽  
Maize Yields ◽  
Total Seed

SUMMARYThe effect of variations in the relative sowing time and density of component crops in a maize/cowpea intercrop were examined in two experiments. In the first experiment, maize and cowpea were sown together, and either 10 or 21 days before or after each other. Maize yield was reduced when sown at the same time or after cowpea; intercrop cowpea yields were less than sole cowpea yields at all sowings. In the second experiment, maize densities of 35, 50 and 70 × 103plants ha−1were combined with cowpea densities of 70, 100 and 140 × 103plants ha−1. Increasing the density of either crop in the mixture resulted in increases in total yield. Maize reduced cowpea yields more than the effect of cowpea on maize yields. In terms of LER and total seed protein yield, there was no advantage of either staggered sowings over simultaneous sowing or of the various intercrop density combinations, except between the lowest and the highest densities of either maize or cowpea. The LERs appeared to follow the trends in cowpea yields and total seed protein yields the trends in maize yields.

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