Molecular and Biochemical Study of Seven Pea (Pisum sativum L.) Cultivars through Using RAPDs, SSRs and Total Seed Protein by SDS-PAGE

Author(s):  
Attyaf Jameel Thamir Al-Tamimi
Author(s):  
Anirban Paul ◽  
Koyel Das Bairagya ◽  
Nirmalya Banerjee ◽  
Anjalika Roy

Background: This research work describes about toxicity estimation of commonly used fungicide copper oxychloride using Pisum sativum L. and Vigna radiata L. as a bioindicator. Methods: The seeds of P. sativum and V. radiata were treated with different concentrations of fungicide. Biological toxicity measured by seed germination percentage, R/P ratio, total leaf chlorophyll, total leaf proline, total seed protein, mitotic index, abnormality index and mitotic inhibition. Result: Linear regression analysis showed that seed germination percentage, total seed protein, abnormality index and mitotic inhibition show positive correlation with increasing concentration of fungicide copper oxychloride. The R/P ratio of 4th and 8th days, mitotic index, total leaf chlorophyll and leaf proline have negative correlation along increasing concentration of fungicide. However total leaf proline of V. radiata at 2% concentration of fungicide is abruptly higher than control and other concentrations. The application of copper oxychloride at lower concentration can be used as a safe fungicide.


2003 ◽  
Vol 16 (1) ◽  
pp. 35 ◽  
Author(s):  
Alicia L. Lamarque ◽  
Renée H. Fortunato

Total seed proteins of 10 Acacia species were examined by SDS–PAGE. The protein patterns showed qualitative and quantitative differences among the taxa analysed. The main protein components of most species examined had MW's in the range of 38.5–49.0 × 103. Subgenus Aculeiferum differed from subg. Acacia in the presence of a high concentration of proteins in the range of 20–24.5 × 103. Hierarchical clustering of the 10 taxa was undertaken, based on Jaccard distances calculated from electrophoretic data. The species grouped in two main clusters, representing the two subgenera of Acacia that occur in America, namely subg. Acacia and subg. Aculeiferum. The taxonomic placement of Acacia emilioana, a species with uncertain sectional affinity within subg. Aculeiferum, is discussed.


Author(s):  
Dragana Obreht ◽  
Ljiljana Vapa ◽  
Sándor Kis ◽  
Mária-Hajos Takács ◽  
Éva-Bányai Stefánovics ◽  
...  

Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.


2004 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Rehana Asghar ◽  
Rabia Siddique . ◽  
Muhammad Afzal . ◽  
Shamim Akhtar .

2021 ◽  
Author(s):  
Dipak Santra ◽  
Kaustav Majumder ◽  
Amit Mitra ◽  
Bijesh Maharjan

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