Role of Some Vitamins in Decrease of Soybean Seed Deterioration During Storage

2014 ◽  
Vol 7 (4) ◽  
pp. 97-115 ◽  
Author(s):  
M.A. El-Metwall ◽  
Aml E.A. El-Saidy ◽  
K.M. Abd El-Hai
Keyword(s):  
Soybean Seed ◽  
Seed Deterioration

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

scholarly journals Biochemical Profile of the Soybean Seed Embryonic Axis and Its Changes during Accelerated Aging

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 186
Author(s):  
Luciano Antônio Ebone ◽  
Andréia Caverzan ◽  
Diógenes Cecchin Silveira ◽  
Luciano de Oliveira Siqueira ◽  
Nadia Canali Lângaro ◽  
...  
Keyword(s):  
Ascorbate Peroxidase ◽  
Cell Structure ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Embryonic Axis ◽  
Nutrient Analysis ◽  
Maillard Reactions ◽  
Seed Deterioration ◽  
Randomized Experimental Design ◽  
Peroxidase Enzymes

Seed deterioration is an important topic in plant science, as the majority of cultivated species use seeds as their means of propagation; however, due to its complexity, the process of seed deterioration has not yet been completely elucidated. Three soybean cultivars (BMX Raio, BMX Zeus, and DM 53i54) exposed to four distinct periods of accelerated aging (0, 3, 6 and 9 days) in a fully randomized experimental design. Initially, vigor and germination tests were performed. The activity of superoxide dismutase, catalase, ascorbate peroxidase enzymes, hydrogen peroxide, malonaldehyde, DNA oxidation, macromolecules and mineral content, and Maillard reactions were quantified in the embryonic axis. Results showed that DNA did not suffer degradation or oxidation. In terms of consumption of reserves, only sugars were consumed, while levels of protein, starch, and triglycerides were maintained. The Maillard reaction did show potential as an indicator of buffer capacity of protein to ROS. Additionally, levels of catalase and ascorbate peroxidase decreased during the aging process. Moreover, nutrient analysis showed that a high magnesium level in the cultivar bestowed greater resilience to deterioration, which can indicate a potential function of magnesium in the cell structure via reflex in seed aging through seed respiration.

A transcriptomic analysis reveals soybean seed pre-harvest deterioration resistance pathways under high temperature and humidity stress

Genome ◽  
2020 ◽  
Vol 63 (2) ◽  
pp. 115-124 ◽  
Author(s):  
Yingjie Shu ◽  
Yuli Zhou ◽  
Kebin Mu ◽  
Huimin Hu ◽  
Ming Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Temperature ◽  
Soybean Seed ◽  
Seed Vigor ◽  
Resistant Cultivar ◽  
Soybean Cultivars ◽  
Seed Deterioration ◽  
Temperature And Humidity ◽  
Different Response ◽  
Shock Proteins

Pre-harvest soybean seeds in the field are susceptible to high temperature and humidity (HTH) stress, leading to pre-harvest seed deterioration, which will result in a reduction in grain quality, yield, and seed vigor. To understand the gene expression involved in seed deterioration response under HTH stress, in this study, we conducted an RNA-Seq analysis using two previously screened soybean cultivars with contrasting seed deterioration resistance. HTH stress induced 1081 and 357 differentially expressed genes (DEGs) in the sensitive cultivar Ningzhen No. 1 and resistant cultivar Xiangdou No. 3, respectively. The majority of DEGs in the resistant cultivar were up-regulated, while down-regulated DEGs were predominant in the sensitive cultivar. KEGG pathway analysis revealed that metabolic pathways, biosynthesis of secondary metabolites, and protein processing in endoplasmic reticulum were the predominant pathways in both cultivars during seed deterioration under HTH stress. The genes involved in photosynthesis, carbohydrate metabolism, lipid metabolism, and heat shock proteins pathways might contribute to the different response to seed deterioration under HTH treatment in the two soybean cultivars. Our study extends the knowledge of gene expression in soybean seed under HTH stress and further provides insight into the molecular mechanism of seed deterioration as well as new strategies for breeding soybean with improved seed deterioration resistance.

scholarly journals Role of H-1 and H-2 Subunits of Soybean Seed Ferritin in Oxidative Deposition of Iron in Protein

2010 ◽  
Vol 285 (42) ◽  
pp. 32075-32086 ◽  
Author(s):  
Jianjun Deng ◽  
Xiayun Liao ◽  
Haixia Yang ◽  
Xiangyu Zhang ◽  
Zichun Hua ◽  
...  
Keyword(s):  
Soybean Seed

scholarly journals Role of Raffinose Family Oligosaccharides in Respiratory Metabolism During Soybean Seed Germination

2019 ◽  
Vol 57 (4) ◽  
pp. 107-112
Author(s):  
Daimon SYUKRI ◽  
Manasikan THAMMAWONG ◽  
Hushna Ara NAZNIN ◽  
Kohei NAKANO
Keyword(s):  
Seed Germination ◽  
Soybean Seed ◽  
Respiratory Metabolism ◽  
Raffinose Family Oligosaccharides

Effects of Ca2+- and Mg2+-enriched germination media on germination and sugar leakage of Ca-deficient soybean seed

1995 ◽  
Vol 75 (2) ◽  
pp. 343-346 ◽  
Author(s):  
J. R. Keiser ◽  
R. E. Mullen ◽  
P. N. Hinz
Keyword(s):  
High Performance ◽  
Seed Quality ◽  
Sugar Content ◽  
Membrane Integrity ◽  
Soybean Seed ◽  
Bathing Solution ◽  
Distilled Water ◽  
Glycine Max L ◽  
Calcium Magnesium

Inadequate seed Ca concentrations are associated with reduced seed quality of soybean [Glycine max (L.) Merr.]. Standard germination and sugar leakage tests were conducted to determine the effect of seed Ca content and exogenous Ca2+ or Mg2+ on seed quality and seed leakage. Soybean seeds containing different Ca levels were germinated in rolled towels that had been soaked in distilled water, 0.01 M Ca(NO3)2, or 0.01 M Mg(NO3)2. Sugar leakage was measured by soaking seed containing 1.10, 1.72 or 2.36 mg g−1 Ca in distilled water, 0.01 M Ca (NO3)2, 0.01 M Mg(NO3)2 or polyethylene glycol (PEG). An aliquot of the bathing solution was analyzed for sugar content after 6, 12 and 24 h of soaking, by using high-performance liquid chromatography. As seed Ca content decreased from 2.36 to 1.10 mg g−1, germination in distilled water decreased from 87 to 56%. Addition of either Ca(NO3)2 or Mg(NO3)2 to the medium resulted in increased germination of low-Ca seed, but Ca(NO3)2 was more effective in improving germination of low-Ca seed than Mg(NO3)2. Total sugar leakage was significantly greater in low-Ca seed than in high-Ca seed. Bathing solutions of Ca(NO3)2 or Mg(NO3)2 significantly decreased sugar leakage, compared with distilled water or PEG. These results suggest that the role of Ca in seed germination may involve membrane stabilization. They also indicate that the addition of Ca(NO3)2 or Mg(NO3)2 to the germination medium may improve germination of Ca-deficient seed. Key words: Soybean, germination, calcium, magnesium, sugar leakage, membrane integrity

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