scholarly journals Extracellular laccase production and phenolic degradation by an olive mill wastewater isolate

2018 ◽  
Vol 69 (1) ◽  
pp. 231 ◽  
Author(s):  
R. Kumar ◽  
Y. Raizner ◽  
L. I. Kruh ◽  
O. Menashe ◽  
H. Azaizeh ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Antimicrobial Agents ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Laccase Production ◽  
16S Rna ◽  
Extracellular Laccase ◽  
Acinetobacter Pittii ◽  
Constitutive Production ◽  
Olive Mill

Olive mill wastewater (OMWW) presents a challenge to the control of effluents due to the presence of a high organic load, antimicrobial agents (monomeric-polymeric phenols, volatile acids, polyalcohols, and tannins), salinity and acidity. In this study, the production of extracellular laccase, monomeric or polymeric phenol, from an OMWW isolate based on its ability to biodegrade phenols and gallic acid as a model of phenolic compounds in OMWW was investigated. Phylogenetic analysis of the 16S RNA gene sequences identified the bacterial isolate (Acinetobacter REY) as being closest to Acinetobacter pittii. This isolate exhibited a constitutive production of extracellular laccase with an activity of 1.5 and 1.3 U ml/L when supplemented with the inducers CuSO4 and CuSO4+phenols, respectively. Batch experiments containing minimal media supplemented with phenols or gallic acid as the sole carbon and energy source were performed in order to characterize their phenolic biodegradability. Acinetobacter REY was capable of biodegrading up to 200 mg/L of phenols and gallic acid both after 10 h and 72 h, respectively.

scholarly journals Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

2021 ◽  
Vol 11 (3) ◽  
pp. 1293
Author(s):  
Ana Eusébio ◽  
André Neves ◽  
Isabel Paula Marques
Keyword(s):  
Anaerobic Digestion ◽  
Microbial Communities ◽  
Volatile Fatty Acids ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Olive Mill

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.

Panus tigrinus efficiently removes phenols, color and organic load from olive-mill wastewater

2004 ◽  
Vol 155 (7) ◽  
pp. 596-603 ◽  
Author(s):  
Alessandro D'Annibale ◽  
Marika Ricci ◽  
Daniele Quaratino ◽  
Federico Federici ◽  
Massimiliano Fenice
Keyword(s):  
Olive Mill Wastewater ◽  
Organic Load ◽  
Panus Tigrinus ◽  
Olive Mill

scholarly journals Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Ahmed Tafesh ◽  
Naim Najami ◽  
Jeries Jadoun ◽  
Fares Halahlih ◽  
Herbert Riepl ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Growth Inhibition ◽  
Gallic Acid ◽  
Pathogenic Bacteria ◽  
Olive Mill Wastewater ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Olive Mill

Polyphenols or phenolic compounds are groups of secondary metabolites widely distributed in plants and found in olive mill wastewater (OMW). Phenolic compounds as well as OMW extracts were evaluatedin vitrofor their antimicrobial activity against Gram-positive (Streptococcus pyogenesandStaphylococcus aureus) and Gram-negative bacteria (Escherichia coliandKlebsiella pneumoniae). Most of the tested phenols were not effective against the four bacterial strains when tested as single compounds at concentrations of up to 1000 μg mL−1. Hydroxytyrosol at 400 μg mL−1caused complete growth inhibition of the four strains. Gallic acid was effective at 200, and 400 μg mL−1againstS. aureus, andS. pyogenes, respectively, but not against the gram negative bacteria. An OMW fraction called AntiSolvent was obtained after the addition of ethanol to the crude OMW. HPLC analysis of AntiSolvent fraction revealed that this fraction contains mainly hydroxytyrosol (10.3%), verbascoside (7.4%), and tyrosol (2.6%). The combinations of AntiSolvent/gallic acid were tested using the low minimal inhibitory concentrations which revealed that 50/100–100/100 μg mL−1caused complete growth inhibition of the four strains. These results suggest that OMW specific fractions augmented with natural phenolic ingredients may be utilized as a source of bioactive compounds to control pathogenic bacteria.

Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater

2006 ◽  
Vol 81 (9) ◽  
pp. 1556-1562 ◽  
Author(s):  
Aurora Rizzi ◽  
Mauro Zucchi ◽  
Sara Borin ◽  
Massimo Marzorati ◽  
Claudia Sorlini ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Load Increase ◽  
Olive Mill

scholarly journals Sorbents Coupled to Solar Light TiO2-Based Photocatalysts for Olive Mill Wastewater Treatment

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Andrea Speltini ◽  
Federica Maraschi ◽  
Michela Sturini ◽  
Valentina Caratto ◽  
Maurizio Ferretti ◽  
...  
Keyword(s):  
Activated Carbons ◽  
Uv Light ◽  
Sol Gel ◽  
Oxygen Demand ◽  
Integrated Approach ◽  
Olive Mill Wastewater ◽  
Solar Light ◽  
Organic Load ◽  
One Pot ◽  
Olive Mill

The aim of this work was to couple physical-chemical approaches with photocatalysis to reduce by a simple, inexpensive way the organic load of olive mill wastewater (OMW), mandatorily prior to the final discharge. Before irradiation, different sorbents were tested to remove part of the organic fraction, monitored by measuring chemical oxygen demand (COD) and polyphenols (PP). Different low-cost, safe materials were tested, that is, Y zeolite (ZY), montmorillonite, and sepiolite. Considerable decrease of organic load was obtained, with the highest abatement (40%) provided by ZY (10 g L−1in 1 : 10 OMW). Use of the three sorbents, in particular ZY, was convenient compared to commercial activated carbons. UV light photocatalytic tests, performed using P25 TiO2on ZY-treated OMW, yielded quantitative remediation (ca. 90%). Also solar light provided significative results, PP being lowered by 74% and COD by 56%. Sol-gel anatase TiO2and N-doped anatase TiO2were also tested, obtaining good results, around-80% PP and-40% COD. Finally, an integrated approach was experimented by ZY-supported anatase TiO2(TiO2@ZY). Thisphotoreactive sorbentallowedone-pottreatment of OMW significative abatements of PP (-77%) and COD (-39%) with only 1 g L−1material, under solar light.

Influence of the heavy metals concentration on the anaerobic co-digestion performance of activated sludge and Olive mill wastewater

2021 ◽  
Author(s):  
khalideh Al bkoor Alrawashdeh
Keyword(s):  
Heavy Metals ◽  
Activated Sludge ◽  
Removal Efficiency ◽  
Biogas Production ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
High Concentration ◽  
Digestion Process ◽  
The Impact ◽  
Olive Mill

Abstract The influence of heavy metals (HMs) on the anaerobic co-digestion process (AD) of activated sludge and olive mill wastewater (50% v/v) was studied. Biogas production, methane CH4 concentration, and removal efficiency of the TS, VS, and TCOD were investigated in mesophilic conditions. The toxicity and inhibitory effects of HMs at different concentrations (15–60 ppm) on the digestion process were specified.A high concentration of HMs has resulted in a significant decrease in AD performance in terms of organic load degradation, biogas production, CH4 content, TCOD removal efficiency, and inhibition of hydrogenotrophic-methanogenic bacteria. The toxicity of HMs can be arranged according to TS removal: Cu > Zn ≈ Cr > Pb, according to TCOD removal efficiency: Cu > Cr > Zn > Pb, biogas production: Cu > Zn ≈ Cr > Pb, CH4 content: Cu > Zn > Cr > Pb. Also, the results showed that the methanogenic stage was influenced negatively and more than the acetogenic stage, where Pb < 30 ppm had a lower inhibitor effect on the digestion, while the lowest concentration of Cu(II) leads to the significant inhibition of the AD process is ≥ 10 ppm. At the concentration of 60 ppm of Pb, Zn, Cr, and Cu, the COD removal efficiency was 17.07%, 15.64%, 19.13%, and 20.53% respectively, TS removal was 17.31%, 13.44%, 16.28%, and 10.37 respectively, the VS removal was 19.4%, 14.445, 7.94% and 5.17%, respectively. Also, at the concentration of 60 ppm, biogas production has decreased by 51.55%, 66.46%, 68.1%, and 73.91%, respectively.Novelty statement This study provides new data specifying the inhibitor HMs concentration and the impact of HMs at the various concentrations on the anaerobic co-digestion of active sludge and olive mill wastewater

scholarly journals Effects of High Voltage Electrical Discharge Plasma on Olive Mill Wastewater Treatment

2021 ◽  
Vol 13 (3) ◽  
pp. 1552
Author(s):  
Mia Ivanov ◽  
Tomislava Vukušić Pavičić ◽  
Klara Kraljić ◽  
Dijana Grgas ◽  
Tibela Landeka Dragičević ◽  
...  
Keyword(s):  
Organic Compounds ◽  
High Voltage ◽  
Electrical Discharge ◽  
Cold Plasma ◽  
Discharge Plasma ◽  
Color Change ◽  
Removal Rate ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Olive Mill

High voltage electrical discharge plasma technology (HVED) is considered as a promising technology for wastewater remediation due to its fast removal rate and environmental compatibility. Olive mill wastewater (OMWW) treatment presents crucial environmental issues because of its high organic load and intense toxicity and phytotoxicity. The effect of cold plasma at frequencies of 60 Hz and 120 Hz, with injected gas (air, oxygen and nitrogen) and with and without the addition of FeCl3x6H2O, during 30 min, on degradation and removal of organic compounds, as well as polyphenols from OMWW, were investigated. The efficiency of cold plasma was monitored by pH, temperature, electroconductivity, redox potential, oxygen saturation and reduction of chemical oxygen demand (COD) and polyphenols. The best removal efficiency of 50.98% of organic compounds was achieved at 120 Hz with nitrogen and the addition of FeCl3x6H2O, and a 60.32% reduction of polyphenols at a frequency of 60 Hz with the air and FeCl3x6H2O added was obtained. Also, the plasma treatment resulted in a decrease in coloring intensity, with the most significant color change at 120 Hz with the addition of FeCl3x6H2O with nitrogen and air.

scholarly journals Advanced oxidation treatments of olive mill wastewater

2019 ◽  
Vol 6 (2) ◽  
pp. 71-78
Author(s):  
Mia Ivanov ◽  
Katarina Perić ◽  
Tomislava Vukušić ◽  
Zoran Herceg ◽  
Tibela Landeka Dragičević ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Organic Compounds ◽  
Uv Radiation ◽  
High Intensity ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Ecological Problem ◽  
High Intensity Ultrasound ◽  
Dissolved Carbon ◽  
Olive Mill

New and innovative advanced oxidative processes for wastewater treatments are currently in the focus of scientific research and development for possible industrial implantation. The main aim of this study was to investigate the effect of cold plasma treatment, high intensity ultrasound and UV radiation with the addition of additives: H2O2, TiO2, FeCl3 x 6H2O on degradation and removal of complex organic compounds from olive mill wastewater (OMWW). Olive mill wastewater represents a potential ecological problem when it is raw disposed into the environment, because of its high organic load. OMWW samples (with and without additives) were treated by high-voltage plasma discharge at frequencies 60 Hz and 120 Hz in combination with pumped gases (nitrogen, air and oxygen) for 30 minutes, by UV radiation for 30 minutes and 10 minutes by high intensity ultrasound. Physico-chemical parameters of quality, chemical oxygen demand (COD), and total dissolved carbon (TOC) were determined. The results have shown the efficacy of plasma treatment in degradation of organic compounds as well as degradation and reduction of polyphenolic compounds. Reduction of colour and total dissolved carbon occurred in all treated samples, mostly with the addition of FeCl3 x 6H2O. Treatment with UV radiation and ultrasound proved to be the most efficient resulting in the 50% reduction of organic compounds after a 10-minute treatment.

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