scholarly journals Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Ahmed Tafesh ◽  
Naim Najami ◽  
Jeries Jadoun ◽  
Fares Halahlih ◽  
Herbert Riepl ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Growth Inhibition ◽  
Gallic Acid ◽  
Pathogenic Bacteria ◽  
Olive Mill Wastewater ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Olive Mill

Polyphenols or phenolic compounds are groups of secondary metabolites widely distributed in plants and found in olive mill wastewater (OMW). Phenolic compounds as well as OMW extracts were evaluatedin vitrofor their antimicrobial activity against Gram-positive (Streptococcus pyogenesandStaphylococcus aureus) and Gram-negative bacteria (Escherichia coliandKlebsiella pneumoniae). Most of the tested phenols were not effective against the four bacterial strains when tested as single compounds at concentrations of up to 1000 μg mL−1. Hydroxytyrosol at 400 μg mL−1caused complete growth inhibition of the four strains. Gallic acid was effective at 200, and 400 μg mL−1againstS. aureus, andS. pyogenes, respectively, but not against the gram negative bacteria. An OMW fraction called AntiSolvent was obtained after the addition of ethanol to the crude OMW. HPLC analysis of AntiSolvent fraction revealed that this fraction contains mainly hydroxytyrosol (10.3%), verbascoside (7.4%), and tyrosol (2.6%). The combinations of AntiSolvent/gallic acid were tested using the low minimal inhibitory concentrations which revealed that 50/100–100/100 μg mL−1caused complete growth inhibition of the four strains. These results suggest that OMW specific fractions augmented with natural phenolic ingredients may be utilized as a source of bioactive compounds to control pathogenic bacteria.

scholarly journals Enrichment of Phenolic Compounds from Olive Mill Wastewater and In Vitro Evaluation of Their Antimicrobial Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Saleh Abu-Lafi ◽  
Mahmoud Sami Al-Natsheh ◽  
Reem Yaghmoor ◽  
Fuad Al-Rimawi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Acid Value ◽  
Antimicrobial Activities ◽  
Olive Mill Wastewater ◽  
Total Phenolic ◽  
The Stability ◽  
Olive Mill

The production of olive oil generates massive quantities of by-product called olive mill wastewater (OMWW). The uncontrolled disposal of OMWW poses serious environmental problems. The OMWW effluent is rich in several polyphenolic compounds. Liquid-liquid extraction of OMWW using ethyl acetate solvent was used to enrich phenolic compounds under investigation. Total phenolic and flavonoid content and antioxidant activity of the extract were determined. HPLC coupled to photodiode array (PDA) detector was used to analyze the main three phenolic compounds of OMWW, namely, hydroxytyrosol, tyrosol, and oleuropein. The antimicrobial activity of the extract was also investigated. Additionally, the OMWW extract was used as natural preservative and antioxidants for olive oil. Results showed that OMWW is very rich in phenolic compounds and has strong antioxidant activity. HPLC analysis showed that the extract contains mainly hydroxytyrosol and tyrosol but no oleuropein. The OMWW extract showed also positive activities as antibacterial (gram positive and gram negative) and antifungal as well as activities against yeast. The addition of OMWW extract to olive oil samples has an effect on the stability of olive oil as reflected by its acid value, peroxide value, K232 and K270, and total phenolic content.

Synergistic Activity of Phenolic Compounds of Some Plants Against Bacteria

2021 ◽  
Vol 8 (6) ◽  
pp. 102-110
Author(s):  
Roaa M. H. Shoker
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Synergistic Effect ◽  
Pathogenic Bacteria ◽  
Total Concentration ◽  
Sesamum Indicum ◽  
Gram Negative Bacteria ◽  
Synergistic Activity ◽  
Gram Negative ◽  
Phenolic Extracts

Medicinal plants produce great groups of secondary metabolites which are essential for medicine purpose, one of them phenolic compounds, antimicrobial activity of phenolic compounds which derivative from plants has been examined for several years. The phenolic extracts of Sesamum indicum and Pimpinella anisum seeds have antibacterial action against Gram positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), (Acinetobacter baumannii), and (Pseudomonas aeruginosa) (Proteus mirabilis). The current findings show that the synergistic impact of phenolic extracts from S. indicum and P. anisum is active against a variety of pathogenic bacteria, and that the synergistic effect for two plants is more antibacterial than phenolic extracts from one plant.  The results indicated Gram- negative (P. aeruginosa) more effected by plants, than Gram-negative (S. aureus) which have the lower effects. The results of HPLC indicated Sesame (S. indicum) have total concentration of phenolic compounds was (1313.7 µg/ml) higher than total concentration of phenolic compounds of Anise (P. anisum) (220.991 µg/ml), and have varied types of phenolic compounds were Pyrogallol, Gallic acid, Rutin, Kaempferol, Cinnamaldehyde, Qurctin, Eugenol, Lignan with different concentration. From this study may be conclusion Synergistic effect for two plants have more antibacterial than phenolic extracts of one plant, and Sesame (S. indicum) have higher antimicrobial activity than Anise (P. anisum).

scholarly journals Photoactive Porphyrin Derivative with Broad-Spectrum Activity against Oral Pathogens In Vitro

2000 ◽  
Vol 44 (12) ◽  
pp. 3364-3367 ◽  
Author(s):  
C. R. Rovaldi ◽  
A. Pievsky ◽  
N. A. Sole ◽  
P. M. Friden ◽  
D. M. Rothstein ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Pathogenic Bacteria ◽  
Vitro Activity ◽  
Chlorin E6 ◽  
Periodontal Pocket ◽  
Gram Negative Bacteria ◽  
In Vitro Activity ◽  
Gram Negative ◽  
Porphyrin Derivative

ABSTRACT Photodynamic therapy (PDT) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. PDT is a potential adjunct to scaling and root planing in the treatment of periodontal disease. However, the effectiveness of porphyrin derivatives against microorganisms has been limited because some gram-negative bacteria are refractory to photodynamic treatment with these agents. We have designed a porphyrin derivative conjugated to a pentalysine moeity that endows the molecule with activity against gram-positive and gram-negative bacteria. Whereas the porphyrin, chlorin e6, showed in vitro activity against a limited spectrum of bacteria, chlorin e6 conjugated to pentalysine showed in vitro activity against all oral microorganisms tested, including Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Campylobacter rectus, Eikenella corrodens, Fusobacterium nucleatumsubsp. polymorphum, Actinomyces viscosus, and the streptococci. Potent antimicrobial activity (≥5-log-unit reduction in the numbers of CFU per milliliter) was retained in the presence of up to 25% whole sheep blood. The use of potent, selective agents such as this chlorin e6–pentalysine conjugate to more effectively reduce the pathogenic bacteria in the periodontal pocket may be a significant tool for the treatment of periodontal disease.

scholarly journals In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters

2020 ◽  
Vol 17 (9) ◽  
pp. 3332 ◽  
Author(s):  
Jacek Piatek ◽  
Hanna Krauss ◽  
Arleta Ciechelska-Rybarczyk ◽  
Malgorzata Bernatek ◽  
Paulina Wojtyla-Buciora ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Selection Process ◽  
In Vitro Growth ◽  
Bacterial Strains ◽  
Direct Inhibition ◽  
E Coli ◽  
Beneficial Effects ◽  
Different Types

A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans. Among these is a direct inhibition of the growth of pathogenic bacteria in the gut. The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic. In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.) sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile were determined. Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.) boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS). Inhibition of pathogens was moderate by Sac. boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic. Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.

scholarly journals New Schiff bases of 2-(quinolin-8-yloxy)acetohydrazide and their Cu(ii), and Zn(ii) metal complexes: their in vitro antimicrobial potentials and in silico physicochemical and pharmacokinetics properties

2020 ◽  
Vol 18 (1) ◽  
pp. 591-607
Author(s):  
Hanan A. Althobiti ◽  
Sami A. Zabin
Keyword(s):  
Schiff Base ◽  
Metal Complexes ◽  
In Silico ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Schiff Base Ligands ◽  
Gram Positive ◽  
Gram Negative ◽  
Gi Absorption

AbstractThe purpose of this work was to prepare Schiff base ligands containing quinoline moiety and using them for preparing Cu(ii) and Zn(ii) complexes. Four bidentate Schiff base ligands (SL1–SL4) with quinoline hydrazine scaffold and a series of mononuclear Cu(ii) and Zn(ii) complexes were successfully prepared and characterized. The in vitro antibacterial and antifungal potential experimentation revealed that the ligands exhibited moderate antibacterial activity against the Gram-positive bacterial types and were inactive against the Gram-negative bacteria and the fungus strains. The metal complexes showed some enhancement in the activity against the Gram-positive bacterial strains and were inactive against the Gram-negative bacteria and the fungus strains similar to the parent ligands. The complex [Cu(SL1)2] was the most toxic compound against both Gram-positive S. aureus and E. faecalis bacteria. The in silico physicochemical investigation revealed that the ligand SL4 showed highest in silico absorption (82.61%) and the two complexes [Cu(SL4)2] and [Zn(SL4)2] showed highest in silico absorption with 56.23% for both compounds. The in silico pharmacokinetics predictions showed that the ligands have high gastrointestinal (GI) absorption and the complexes showed low GI absorption. The ligands showed a good bioavailability score of 0.55 where the complexes showed moderate to poor bioavailability.

scholarly journals Antibacterial Activity of Floral Petals of Some Angiosperms

2019 ◽  
pp. 1-5 ◽  
Author(s):  
L. Rajanna ◽  
N. Santhosh Kumar ◽  
N. S. Suresha ◽  
S. Lavanya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Assay

The in vitro antibacterial assay was carried out against both Gram positive (B. cerus and S. aureus) and Gram negative (E. coli and K. pneumoniae) bacteria. Floral petals of 20 different species of plants were collected and tested for antibacterial activity. The result showed that the petals were active against both Gram positive and Gram negative. Out of 20 floral petals tested, 19 floral petals exhibited antibacterial activity against selected bacterial strains. The minimal inhibitory zone of floral petal discs against human pathogenic bacteria varies from 2 – 6 mm. Rosa carolina and Ruellia tuberosa showed significance inhibition zone for all the bacterial strains while Lantana camara does not show inhibition zone for any of these pathogenic bacteria.

scholarly journals Synthesis and antimicrobial activity of some new N-(aryl-oxo-alkyl)-5-arylidene-thiazolidine-2,4-diones

2014 ◽  
Vol 79 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Anca Stana ◽  
Brînduşa Tiperciuc ◽  
Mihaela Duma ◽  
Adrian Pîrnău ◽  
Philippe Verité ◽  
...  
Keyword(s):  
Antimicrobial Activities ◽  
Fungal Strain ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Reference Drug ◽  
Gram Negative ◽  
E Coli ◽  
Inhibitory Activities

A series of new 5-(2,6-dichlorobenzylidene)thiazolidine-2,4-dione and 5-(4-methoxy-benzylidene)thiazolidine-2,4-dione derivatives (3a-h and 5a-h) were synthesized starting from 5-arylidene-thiazolidine-2,4-dione and ?-halo-ketones. The structural elucidation of the newly synthesized compounds was based on elemental analysis and spectroscopic data (MS, 1H-NMR, 13C-NMR). The synthesized compounds were screened for their antimicrobial activities against several pathogenic strains of Gram-positive and Gram-negative bacteria and one fungal strain (Candida albicans), assessed in vitro as growth inhibition diameters. Some of them displayed better inhibitory activities than that of the reference drug against the Gram-positive S. aureus, B. cereus, L. monocytogenes bacterial strains, and showed good antifungal activity against C. albicans, while the antibacterial activity against Gram-negative E. coli and S. typhimurium bacterial strains was moderate.

scholarly journals Antibacterial activity of cobalt(II) complexes with some benzimidazole derivatives

2008 ◽  
Vol 73 (12) ◽  
pp. 1153-1160 ◽  
Author(s):  
S.O. Podunavac-Kuzmanovic ◽  
V.M. Leovac ◽  
D.D. Cvetkovic
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Antibacterial Activities ◽  
Benzimidazole Derivatives ◽  
Wall Structure ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

The antibacterial activities of cobalt(II) complexes with two series of benzimidazoles were evaluated in vitro against three Gram-positive bacterial strains (Bacillus cereus, Staphylococcus aureus, and Sarcina lutea) and one Gram-negative isolate (Pseudomonas aeruginosa). The minimum inhibitory concentration was determined for all the complexes. The majority of the investtigated complexes displayed in vitro inhibitory activity against very persistent bacteria. They were found to be more active against Gram-positive than Gram-negative bacteria. It may be concluded that the antibacterial activity of the compounds is related to the cell wall structure of the tested bacteria. Comparing the inhibitory activities of the tested complexes, it was found that the 1-substituted- -2-aminobenzimidazole derivatives were more active than complexes of 1-substituted- 2-amino-5,6-dimethylbenzimidazoles. The effect of chemical structure on the antibacterial activity is discussed.

scholarly journals In vitro antibacterial activity of essential oils from Lamiaceae species

2016 ◽  
Vol 5 (4) ◽  
pp. 141-144
Author(s):  
Vinod Kumar ◽  
◽  
C. S. Mathela ◽  
Amit Panwar ◽  
◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Broad Spectrum ◽  
Antibacterial Effect ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Negative ◽  
In Vitro Antibacterial Activity

Essential oils from Calamintha umbrosa and Nepeta species viz. N. leucophylla; N. hindostana; N. ciliaris and N. clarkei (family Lamiaceae), was tested against six bacterial strains. To evaluate the correlation between the antimicrobial activity and the essential oils, PCA and HCA analysis was done. PCA and HCA analysis of the antibacterial activity revealed that essential oils of Nepeta species had a strong and broad spectrum antibacterial effect against bacterial strains of P. aeruginosa and S. scandidus. The N. leucophylla oil showed higher activity against Gram-negative bacteria P. aeruginosa (10.5 mm, MIC 10 µL/mL) and K. pneumonia (9.1 mm, MIC 45 µL/mL) among all Nepeta oils which may be due to presence of active antimicrobial iridoids compounds.

scholarly journals Antibacterial Effects of Vitamin E: in Vitro Study

2013 ◽  
Vol 7 (2) ◽  
pp. 17-23
Author(s):  
Dalia Abd Al Kader Al-Salih ◽  
Fitua M. Aziz ◽  
Bahir Abdul Razzaq Mshimesh ◽  
Muhanad T. Jehad
Keyword(s):  
Vitamin E ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
The Body ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Antibacterial Effects ◽  
Gram Positive ◽  
Gram Negative

Overuse of antibiotics has become the major factor for the emergence and dissemination of multi-drug resistant strains of several groups of microorganisms and this lead to search for agents that may have antibacterial effects. Vitamin E emerged as an essential, fat-soluble nutrient in the human body and it is essential, because the body cannot manufacture its own vitamin E, so foods and supplements must provide it. The aim of the present study was to evaluate the effect of vitamin E against pathogenic bacteria. Gram positive and gram negative bacteria were selected as the test microorganisms based on their importance in infections. In this study vitamin E used in four concentrations (50,100,200,400) IU/ml. The agar diffusion method was used to determine antibacterial activity. Results showed that gram negative bacteria were shown to be more resistant than gram positive bacteria. The resistance of gram negative bacteria towards antibacterial substances may be related to lipopolysaccharides in their outer membrane.

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