EFFECTS OF FAR INFRARED RAYS ON HYDROGEN PEROXIDE-SCAVENGING CAPACITY

2011 ◽  
Vol 23 (02) ◽  
pp. 99-105 ◽  
Author(s):  
Ting-Kai Leung ◽  
Huey-Fang Shang ◽  
Dai-Chian Chen ◽  
Jia-Yu Chen ◽  
Tsong-Min Chang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Far Infrared ◽  
Fibroblast Cells ◽  
Scavenging Activity ◽  
Osteoblast Cells ◽  
Delayed Senescence ◽  
Infrared Rays ◽  
Scavenging Capacity ◽  
Carrot Extract

Far infrared rays (FIRs) have several proven effects on the human body and are generally considered to be biologically beneficial. In this study, we determined the effect of FIRs on hydrogen peroxide (H2O2) -scavenging activity, which was directly increased by 10.26% after FIR application. Even in the indirect use of FIRs accompanying carrot extract, FIRs still contributed to a 5.48% increase in H2O2 -scavenging activity. We further proved that additional FIR treatment resulted in about 23.02% and 18.77% viability increases of osteoblast cells in the 200 and 800 μM H2O2 , respectively; and about 25.67% and 47.16% viability increases of fibroblast cells in the 25 and 50 μM H2O2 , respectively. Finally, FIR treatment also delayed senescence of detached Railway Beggarticks leaves in H2O2 solution with the concentrations of 10, 100, and 1000 μM. By reviewing past articles related to the effects of oxidative stress from metabolically produced H2O2 , we discuss possible benefits of FIRs for plants and animals.

scholarly journals Preparation, Structural Characterization, and Property Investigation of Gallic Acid-Grafted Fungal Chitosan Conjugate

2021 ◽  
Vol 7 (10) ◽  
pp. 812
Author(s):  
Weslley Souza de Paiva ◽  
Moacir Fernandes Queiroz ◽  
Diego Araujo Sabry ◽  
André Luiz Cabral Monteiro de Azevedo Santiago ◽  
Guilherme Lanzi Sassaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Molecular Weight ◽  
Gallic Acid ◽  
Low Molecular Weight ◽  
Fibroblast Cells ◽  
Chitosan Derivative ◽  
Promising Agent ◽  
Fungal Chitosan ◽  
Total Antioxidant

Oxidative stress is the cause of numerous diseases in humans; therefore, there has been a continuous search for novel antioxidant molecules. Fungal chitosan is an attractive molecule that has several applications (antifungal, antibacterial, anticancer and antiparasitic action) owing to its unique characteristics; however, it exhibits low antioxidant activity. The aim of this study was to obtain fungal chitosan (Chit-F) from the fungus Rhizopus arrhizus and synthesize its derivative, fungal chitosan-gallic acid (Chit-FGal), as a novel antioxidant chitosan derivative for biomedical use. A low molecular weight Chi-F (~3.0 kDa) with a degree of deacetylation of 86% was obtained from this fungus. Chit-FGal (3.0 kDa) was synthesized by an efficient free radical-mediated method using hydrogen peroxide (H2O2) and ascorbic acid. Both Chit-F and Chit-FGal showed similar copper chelating activities; however, Chit-FGal was more efficient as an antioxidant, exhibiting twice the total antioxidant capacity than Chi-F (p < 0.05). Furthermore, H2O2 (0.06 M) promoted a 50% decrease in the viabilities of the 3T3 fibroblast cells. However, this effect was abolished in the presence of Chit-FGal (0.05–0.25 mg/mL), indicating that Chit-FGal protected the cells from oxidative damage. These results suggest that Chit-FGal may be a promising agent to combat oxidative stress.

scholarly journals Constituents of French Marigold (Tagetes patulaL.) Flowers Protect Jurkat T-Cells against Oxidative Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Irakli Chkhikvishvili ◽  
Tamar Sanikidze ◽  
Nunu Gogia ◽  
Maia Enukidze ◽  
Marine Machavariani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
T Cells ◽  
Radical Scavenging ◽  
Jurkat Cells ◽  
Cellular Mechanisms ◽  
Jurkat T Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory

The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential ofT. patulacompounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patulacompounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

scholarly journals 3β-Hydroxysteroid-Δ24 Reductase Is a Hydrogen Peroxide Scavenger, Protecting Cells from Oxidative Stress-Induced Apoptosis

Endocrinology ◽  
2008 ◽  
Vol 149 (7) ◽  
pp. 3267-3273 ◽  
Author(s):  
Xiuli Lu ◽  
Fukushi Kambe ◽  
Xia Cao ◽  
Yasuko Kozaki ◽  
Takahide Kaji ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Deletion Mutant ◽  
Terminal Deletion ◽  
Scavenging Activity ◽  
Ros Scavenging ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Induced Apoptosis

3β-Hydroxysteroid-Δ24 reductase (DHCR24) is an endoplasmic reticulum-resident, multifunctional enzyme that possesses antiapoptotic and cholesterol-synthesizing activities. To clarify the molecular basis of the former activity, we investigated the effects of hydrogen peroxide (H2O2) on embryonic fibroblasts prepared from DHCR24-knockout mice (DHCR24−/− mouse embryonic fibroblasts). H2O2 exposure rapidly induced apoptosis, which was associated with sustained activation of apoptosis signal-regulating kinase-1 and stress-activated protein kinases, such as p38 MAPK and c-Jun N-terminal kinase. Complementation of the mouse embryonic fibroblasts by adenovirus expressing DHCR24 attenuated the H2O2-induced kinase activation and apoptosis. Concomitantly, intracellular generation of reactive oxygen species (ROS) in response to H2O2 was also diminished by the adenovirus, suggesting a ROS-scavenging activity of DHCR24. Such antiapoptotic effects of DHCR24 were duplicated in pheochromocytoma PC12 cells infected with adenovirus. In addition, it was found that DHCR24 exerted cytoprotective effects in the tunicamycin-induced endoplasmic reticulum stress by eliminating ROS. Finally, using in vitro-synthesized and purified proteins, DHCR24 and its C-terminal deletion mutant were found to exhibit high H2O2-scavenging activity, whereas the N-terminal deletion mutant lost such activity. These results demonstrate that DHCR24 can directly scavenge H2O2, thereby protecting cells from oxidative stress-induced apoptosis.

Antioxidant effect of homogenetisic acid on hydrogen peroxide induced oxidative stress in human lung fibroblast cells

2005 ◽  
Vol 10 (6) ◽  
pp. 556-563 ◽  
Author(s):  
Kyoung Ah Kang ◽  
Sungwook Chae ◽  
Kyung Hwa Lee ◽  
Rui Zhang ◽  
Myung Sun Jung ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Human Lung ◽  
Antioxidant Effect ◽  
Lung Fibroblast ◽  
Fibroblast Cells ◽  
Human Lung Fibroblast

Therapeutic Nanozyme: Antioxidative and cytoprotective effects of nanoceria against hydrogen peroxide induced oxidative stress in fibroblast cells and in zebrafish

2016 ◽  
Vol 1 (11) ◽  
pp. 2849-2856 ◽  
Author(s):  
Bharat Bhushan ◽  
Soundharapandiyan Nandhagopal ◽  
Rajaretinam Rajesh Kannan ◽  
P. Gopinath
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Fibroblast Cells

scholarly journals Extraction, Phenolic Content and Hydrogen Peroxide Scavenging Capacity of Extracts from Some Honey Samples, Propolis and Bee Pollen

2021 ◽  
Vol 10 (1) ◽  
pp. 27-32
Author(s):  
Zohra Mohammedi
Keyword(s):  
Hydrogen Peroxide ◽  
Radical Scavenging Activity ◽  
Local Population ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Food ◽  
Scavenging Activity ◽  
Bee Pollen ◽  
Scavenging Capacity ◽  
Phenolic Extracts

Honey and propolis is natural food, produced by honey bees (Apis mellifera) and largely used by the local population for its medicinal properties. Our work aims to extract and evaluate the hydrogen peroxide scavenging capacity of different phenolic extracts from some bee products. Phenolic compounds from honey samples, propolis, and bee pollen were extracted by methanol and subjected to radical scavenging activity towards hydrogen peroxide. The results showed the highest values for the total phenolic and total flavonoïd contents in propolis and bee pollen, and a great hydrogen peroxide (H2O2) inhibition (IC50: 0.205 - 2.210 µg/mL) with honey extracts, while sample “multiflower” is the better antioxidant, more than ascorbic acid used as control. The less scavenging activity was observed with the extract from bee pollen (IC50: 39.383 µg/mL). To combat the harmful effects of free radicals, especially reactive oxygen species including hydrogen peroxide, it is important to use phenolic extracts instead of using honey as it is, and extracts from the honey of different types are excellent antioxidants compared to other bee products.

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