scholarly journals Socio-environmental and microbiological assessment of the river Piancó, Pombal, Brazil

2016 ◽  
Vol 38 (2) ◽  
pp. 187
Author(s):  
Eulina Kelly Guilherme Lacerda ◽  
Edevaldo Da Silva ◽  
Rosália Severo Medeiros

The River Piancó in the municipality of Pombal on the western stretch of the state of Paraíba, Brazil, receives contaminated effluents. Current assay evaluates the microbiological quality of the river water and analyzes the social and environmental aspects of the riverine community with regard to the water and its use of the river. The water´s microbiological quality was verified at four sites on the river in five two-monthly collections throughout the year (totaling 20 samples) for the following analyses: Most Probably Number (MPN), Total (TC) and Fecal Coliform (FC) and identification of Escherichia coli. The social and environmental aspects were assessed by a 15-question questionnaire given to thirty homes. Results report Fecal Coliforms (> 240 to > 1,100 MPN 100 mL-1), Total (> 93 to > 1,100 MPN 100 mL-1) and high concentrations of E. coli (> 898 to > 1,100 UFC 100 mL-1) in all the sampling sites. The river is frequented by the riverine people for home consumption, recreation and fishing. Conditions of the River Piancó, especially in the stretch that margins the town of Pombal, are highly critical and exceed the limits of current Brazilian legislation with regard to drinking and swimming conditions. 

1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.


1980 ◽  
Vol 43 (10) ◽  
pp. 769-773 ◽  
Author(s):  
C. R. HACKNEY ◽  
B. RAY ◽  
M. L. SPECK

Microbiological analyses of 716 seafood samples over a 3-year period revealed that the microbiological quality of fresh seafood in North Carolina was generally acceptable. The mean aerobic counts (APC) and fecal coliform counts were low as was the occurrence of enteric pathogens, except for Vibrio parahaemolyticus and coagulase-positive staphylococci. Salmonella species were isolated from three samples, but the fecal coliform counts of these samples far exceeded the shellfish standard of the United States Food and Drug Administration. Coagulase-positive staphylococci were isolated in low numbers from nearly all the different types of seafoods; unpasteurized crabmeat and head-peeled shrimp samples showed the highest counts (10% of these samples had numbers which exceeded 100/g). V. parahaemolyticus occurred frequently in fresh seafood (overall 46% of the samples were positive) and its numbers showed a definite seasonal variation. No positive statistical correlation was found between the numbers of V. parahaemolyticus and the bacteriological indices, such as coliforms, fecal coliforms, enterococci and APC. Processing practices were found to influence the occurrence of V. parahaemolvticus in seafood; for example, improperly cleaned flumes were found to be a reservoir for V. parahaemolyticus in mechanical scallop processing plants. Also ‘picking’ waste ‘containers’ were found to be sources of V. parahaemolyticus in crab processing plants. Some processing practices such as heat shocking of oysters to facilitate opening were found to reduce the numbers of V. parahaemolyticus. Fifty V. parahaemolyticus isolates from different seafoods were tested for their Kanagawa reaction and all were found to be negative.


1970 ◽  
Vol 27 (2) ◽  
pp. 42-45
Author(s):  
AS Mustafa Kamal ◽  
Fauzia Begum ◽  
Abul Khair

The results revealed that 40% of fresh mushrooms, 93% of processed and preserved mushroom samples including mushroom-based food products were safe for human consumption from bacteriological point of view. The highest bacterial load recorded was 8.7 × 108 cfu / gm in the samples collected from Sutrapur area of Dhaka city. The results also indicated that 27, 13, 13 and 7% of the fresh mushrooms were contaminated with coliform, fecal coliform, E. coli and Salmonella sp. respectively. Thus those mushroom samples were not up to the mark for consumption, considering safety and sanitation point of view. In case of processed mushrooms and mushroom-based food products the results showed that 20% of dried mushrooms were contaminated with both coliform and fecal coliform, 7% of powdered mushrooms were contaminated with only coliform and 13% of mushroom soup powder were contaminated with coliform, E. coli and Salmonella sp. In case of preserved mushrooms, it was found that 7% of the canned mushrooms had count <10 cfu/gm which was unacceptable as per specifications for the canned food. However, the results also revealed that coliform, fecal coliform, E. coli and even Salmonella sp. were not detected in preserved mushrooms examined.DOI: http://dx.doi.org/10.3329/bjm.v27i2.9170 BJM 2010; 27(2): 42-45


1986 ◽  
Vol 32 (11) ◽  
pp. 891-893 ◽  
Author(s):  
Donald W. Warburton ◽  
Pearl I. Peterkin ◽  
Karl F. Weiss ◽  
Murray A. Johnston

The microbiological quality of bottled water sold in Canada was evaluated. A total of 114 lots of bottled water, both domestic and imported, were analyzed for aerobic colony count, coliforms, fecal coliforms, and Escherichia coli. No fecal coliforms or E. coli were found. Nineteen (46%) of the 41 lots of domestic purified water were found to exceed aerobic colony count standards and another lot exceeded coliform standards. One lot each of domestic and imported mineral water exceeded coliform standards. If mineral water were governed by the aerobic colony count standards for bottled water, then five lots each of both domestic and imported mineral water would have been found to be unsatisfactory. More surveillance of the bottled water industry in Canada is recommended.


2019 ◽  
Vol 4 (1) ◽  
pp. 65
Author(s):  
Sandna Larissa Freitas Santos ◽  
Leandro Lima de Vasconcelos ◽  
Rogério Nunes dos Santos

Os intensos períodos de estiagem e pela concentração que a água adquire quando os níveis dos reservatórios estão baixos torna-se evidente a ausência da qualidade da água consumida pela população. O estudo teve como objetivo avaliar os parâmetros físico-químicos e microbiológicos da água para consumo no município de Itapiúna-CE. Estudo do tipo experimental, prospectivo, transversal e com abordagem do tipo qualitativo e quantitativo. Foram analisadas 4 amostras de 500 mL cada, uma do Açude Castro e de três locais escolhidos nas residências de pontos estratégicos de distribuição pela concepcionária, da água usada para consumo no município de Itapiúna-CE no mês de outubro de 2016. Para seleção das amostras foram adotados os critérios de inclusão: água do açude castro (Amostra A), e três amostras de residências, consideradas a água retirada diretamente do registro da Cagece, escolhidas aleatoriamente dos bairros do município. As amostras apresentaram cloretos alterados quando comparadas às quantidades estabelecidas pela Portaria 2914/2011 do Ministério da Saúde (MS). A respeito aos parâmetros como Sólidos Totais, Condutividade, Cloreto e Dureza, os valores também encontrou-se valores superiores. A amostra A (Açude Castro) obteve pH de 8.81 e apresentou alcalinidade devido a carbonatos (80 mgCO3/L) e a bicarbonatos (150mg CaCO3/L). Verificou-se que 50% das amostras apresentaram presença de coliformes totais e coliformes fecais, evidenciando contaminação da maioria das amostras analisadas. Contudo, de acordo com as análises realizadas, as amostras não obedecem ao padrão de potabilidade, tornando á água imprópria para o consumo humano. PHYSICAL-CHEMICAL AND MICROBIOLOGICAL QUALITY OF WATER FOR CONSUMPTION IN RESIDENCES OF A TOWN IN THE SERTÃO CENTRAL ABSTRACT Factors such as long periods of drought and the physical and chemical characteristics of water influence in the quality of water consumed by the population, leading to health problems. This study aimed at evaluating the physical-chemical and microbiological parameters of water for consumption in the town of Itapiúna-CE. Four samples of 500 mL each were analyzed, one from Açude Castro and three from chosen places in the residences of strategic sites of distribution by the water company for consumption in the town of Itapiúna-CE in October 2016. For sample selection it was adopted the inclusion criteria: water from Açude Castro (sample A), and three samples of residences, considered the water withdrawn directly from Cagece register, randomly chosen from neighborhoods of the town. The samples present altered chlorides compared to the established quantities by the Consolidation Ordinance No. 5 of September 28, 2017 of the Ministry of Health (MS). Regarding the parameters such as total solids, conductivity, chloride and hardness, were also found higher values. Sample A (Açude Castro) obtained pH of 8.81 and presented alkalinity due to carbonates (80 mgCO3 / L) and bicarbonates (150 mg CaCO3 / L). It was verified that 50% of the samples presented total coliforms and fecal coliforms, evidencing contamination of most of the samples analyzed. So, according to the analysis carried out, the samples did not obey the standard of potable water, making the water unfit for human consumption.


1969 ◽  
Vol 69 (1) ◽  
pp. 91-97
Author(s):  
F. Fernández-Coll

Samples of selected frozen Puerto Rican foods were purchased at the retail level and analyzed microbiologically. There was variation in the microbiological quality of these foods, particularly among brands within a given product. Some samples had high coliform and fecal coliform counts, although no coagulase positive staphylococci or Salmonella was found in any sample tested. Some of these foods were cooked according to processor's instructions and then held warm for up to 4 hr at 38° C to reproduce conditions prevailing in routine practice in some cafeterias and fast food establishments. Results demonstrated that total bacterial counts were greatly diminished and in most cases completely eliminated, and that coliforms and fecal coliforms were completely destroyed in all cases by the cooking process. Holding foods warm for 4 hr after cooking did not increase the microbial content of any of the samples. Provided post cooking contamination is avoided, holding these cooked foods at warm temperatures does not affect their microbiological quality.


1987 ◽  
Vol 50 (7) ◽  
pp. 545-549 ◽  
Author(s):  
DIANE PAILLE ◽  
CAMERON HACKNEY ◽  
LAWERENCE REILY ◽  
MARY COLE ◽  
MARILYN KILGEN

Seasonal variation was observed in the type of bacteria which comprised the fecal coliform population of oysters. Escherichia coli was the principal fecal coliform when water temperatures were below 22°C. Conversely, Klebsiella sp. predominated during the summer months. No significant relationship was observed between levels of E. coli and enterococci and non-E. coli fecal coliforms in oysters. Fecal coliform and E. coli levels were significantly (p &gt;0.001) related in water. Klebsiella sp. isolated from oysters demonstrated considerably less multiple antimicrobial agent resistance than clinical isolates of K. pneumoniae. Fecal coliform-positive Klebsiella species had characteristics of environmental organisms. Results of this study suggest that high levels of non-E coli fecal coliforms in oysters harvested in the summer from beds meeting the fecal coliform water standard are not indicative of sewage pollution. Furthermore, it is suggested that the safety indicator in the guideline for oyster meats should be changed form fecal coliforms to E. coli.


Domiati cheese is the most popular brand of cheese ripened in brine in the Middle East in terms of consumed quantities. This study was performed to investigate the impact of the microbiological quality of the used raw materials, the applied traditional processing techniques and ripening period on the quality and safety of the produced cheese. Three hundred random composite samples were collected from three factories at Fayoum Governorate, Egypt. Collected samples represent twenty-five each of: raw milk, table salt, calf rennet, microbial rennet, water, environmental air, whey, fresh cheese, ripened cheese & swabs from: worker hands; cheese molds and utensils; tanks. All samples were examined microbiologically for Standard Plate Count (SPC), coliforms count, Staphylococcus aureus (S. aureus) count, total yeast & mould count, presence of E. coli, Salmonellae and Listeria monocytogenes (L. monocytogenes). The mean value of SPC, coliforms, S. aureus and total yeast & mould counts ranged from (79×102 CFU/m3 for air to 13×108 CFU/g for fresh cheese), (7×102 MPN/ cm2 for tank swabs to 80×106 MPN/ml for raw milk), (9×102 CFU/g for salt to 69×106 CFU/g for fresh cheese) and (2×102 CFU/cm2 for hand swabs to 60×104 CFU/g for fresh cheese), respectively. Whereas, E. coli, Salmonella and L. monocytogenes failed to be detected in all examined samples. There were significant differences in all determined microbiological parameters (p ≤0.05) between fresh and ripened cheese which may be attributed to different adverse conditions such as water activity, pH, salt content and temperature carried out to improve the quality of the product.


1993 ◽  
Vol 27 (7-8) ◽  
pp. 287-294 ◽  
Author(s):  
S. Lerman ◽  
O. Lev ◽  
A. Adin ◽  
E. Katzenelson

The Israel Ministry of Health is now revising its regulations for the assurance of safe water quality in public swimming pools. Since it is not possible to monitor each of the pathogenic microorganisms, it is often recommended to monitor indicator bacteria which provide indirect information on the water quality in the swimming pool. Three indicator microorganisms are often recommended: coliform counts (total coliforms, fecal coliforms or E. Coli), staphylococcus aureus and pseudomonas aeruginosa. A four year survey of the water quality of swimming pools in the Jerusalem District was conducted in order to determine whether the monitoring of all three indicators is necessary to assure safe water quality or is it sufficient to monitor only a single microorganism. A statistical analysis, conducted by using several different statistical techniques, reveals that the populations of the three indicator organisms are significantly interdependent but the correlations between each pair of these indicators are not sufficient to base a prediction of any of the organisms based on the measurements of the others. Therefore, it is concluded that monitoring of all three indicators should be recommended in order to provide an adequate picture of the water quality in swimming pools.


2009 ◽  
Vol 72 (4) ◽  
pp. 856-859 ◽  
Author(s):  
HOIKYUNG KIM ◽  
YOUNGJUN LEE ◽  
LARRY R. BEUCHAT ◽  
BONG-JUNE YOON ◽  
JEE-HOON RYU

Sprouted vegetable seeds used as food have been implicated as sources of outbreaks of Salmonella and Escherichia coli O157:H7 infections. We profiled the microbiological quality of sprouts and seeds sold at retail shops in Seoul, Korea. Ninety samples of radish sprouts and mixed sprouts purchased at department stores, supermarkets, and traditional markets and 96 samples of radish, alfalfa, and turnip seeds purchased from online stores were analyzed to determine the number of total aerobic bacteria (TAB) and molds or yeasts (MY) and the incidence of Salmonella, E. coli O157:H7, and Enterobacter sakazakii. Significantly higher numbers of TAB (7.52 log CFU/g) and MY (7.36 log CFU/g) were present on mixed sprouts than on radish sprouts (6.97 and 6.50 CFU/g, respectively). Populations of TAB and MY on the sprouts were not significantly affected by location of purchase. Radish seeds contained TAB and MY populations of 4.08 and 2.42 log CFU/g, respectively, whereas populations of TAB were only 2.54 to 2.84 log CFU/g and populations of MY were 0.82 to 1.69 log CFU/g on alfalfa and turnip seeds, respectively. Salmonella and E. coli O157:H7 were not detected on any of the sprout and seed samples tested. E. sakazakii was not found on seeds, but 13.3% of the mixed sprout samples contained this potentially pathogenic bacterium.


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