scholarly journals Assessment of Microbiological Quality of Fresh-cut, Processed and Preserved Mushrooms Available in and Around Dhaka City

1970 ◽  
Vol 27 (2) ◽  
pp. 42-45
Author(s):  
AS Mustafa Kamal ◽  
Fauzia Begum ◽  
Abul Khair
Keyword(s):  
Fecal Coliform ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Food Products ◽  
Point Of View ◽  
Human Consumption ◽  
Dhaka City ◽  
Canned Food ◽  
E Coli

The results revealed that 40% of fresh mushrooms, 93% of processed and preserved mushroom samples including mushroom-based food products were safe for human consumption from bacteriological point of view. The highest bacterial load recorded was 8.7 × 108 cfu / gm in the samples collected from Sutrapur area of Dhaka city. The results also indicated that 27, 13, 13 and 7% of the fresh mushrooms were contaminated with coliform, fecal coliform, E. coli and Salmonella sp. respectively. Thus those mushroom samples were not up to the mark for consumption, considering safety and sanitation point of view. In case of processed mushrooms and mushroom-based food products the results showed that 20% of dried mushrooms were contaminated with both coliform and fecal coliform, 7% of powdered mushrooms were contaminated with only coliform and 13% of mushroom soup powder were contaminated with coliform, E. coli and Salmonella sp. In case of preserved mushrooms, it was found that 7% of the canned mushrooms had count <10 cfu/gm which was unacceptable as per specifications for the canned food. However, the results also revealed that coliform, fecal coliform, E. coli and even Salmonella sp. were not detected in preserved mushrooms examined.DOI: http://dx.doi.org/10.3329/bjm.v27i2.9170 BJM 2010; 27(2): 42-45

scholarly journals Microbiological Quality of Kachumbari, a Raw Vegetable Salad Popularly Served Alongside Roast Meat in Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Kenneth M. Mbae ◽  
Mercy K. Ndwiga ◽  
Fredrick G. Kiruki
Keyword(s):  
Cross Section ◽  
Microbiological Quality ◽  
Disease Outbreaks ◽  
Human Consumption ◽  
Microbial Quality ◽  
Protection Agency ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Food Borne

Raw salads are regularly implicated in food-borne disease outbreaks globally. Consumption of kachumbari, a raw vegetable salad, alongside roast meat is widespread in Kenya. The aim of this study was to evaluate the bacteriological quality of kachumbari samples (n=39) collected from a cross section of roasted meat eateries in Kenya. The United Kingdom’s Health Protection Agency guidelines were used to infer safety of the salads due to lack of local criteria for microbiological safety of ready-to-eat fresh produce placed in the market. Based on Escherichia coli counts, 14 (35.9%) of the samples were of satisfactory microbial quality (<20 CFU/g), 7 (17.9%) in the borderline (20–≤102 CFU/g), and 18 (46.2%) unsatisfactory (>102 CFU/g). All samples examined for staphylococci had counts falling within the borderline range (20–≤104 CFU/g). Collectively, 3 (7.7%) of the sampled salads were classified as potentially harmful to health and/or unfit for human consumption due to the presumptive presence of 2 (5.1%) Campylobacter spp. and 1 (2.6%) E. coli O157. Salmonella was not detected in any of the samples. The presence of hygiene indicator microorganisms and pathogens demonstrates that kachumbari salads present a public health risk.

scholarly journals The effect of container-biofilm on the microbiological quality of water used from plastic household containers

2003 ◽  
Vol 1 (3) ◽  
pp. 101-108 ◽  
Author(s):  
P. Jagals ◽  
C. Jagals ◽  
T. C. Bokako
Keyword(s):  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Human Consumption ◽  
Microbiological Contamination ◽  
Supply Source ◽  
E Coli ◽  
Quality Of Water ◽  
Storage Containers

Studies in Southern Africa have shown that even when microbiologically safe water is supplied to developing communities at communal standpipes, contamination by high numbers of pathogenic microorganisms may occur during the processes of fetching water from the supply source and storage during use at home, rendering such waters unsafe for human consumption. This study investigated the occurrence of biofilm in PVC storage containers as one possible reason for this deterioration, using heterotrophic bacteria and total coliform counts as well as turbidity as indicators. A second objective was to determine whether biofilm in water-storage containers could contribute to hazardous microbiological contamination indicated by Escherichia coli and Clostridium perfringens. Results indicated that increased microbiological contamination is associated with biofilm. The biofilm harbours heterotrophic bacteria, total coliforms and C. perfringens. E. coli could not be associated directly with the levels of biofilm in containers but rather appears to be introduced intermittently from the ambient domestic environment. When dislodged with the biofilm, these bacteria contributed substantially to the deterioration of the microbiological quality of supplied water stored in plastic containers.

scholarly journals Microbial study of organisms isolated from nutritional fruit juices surrounded by local fruit market in Nanded, Maharashtra, India

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Ashwini Deshmukh ◽  
Mujahed Siddiqui ◽  
Umar Khan Pathan ◽  
Umesh Dhuldhaj
Keyword(s):  
Bacterial Load ◽  
Food Additives ◽  
Food Products ◽  
Fruit Juices ◽  
Biochemical Tests ◽  
Nutritional Values ◽  
E Coli ◽  
Hidden Hunger ◽  
Microbial Study

Abstract. Deshmukh AS, Siddiqui MM, Pathan UK, Dhuldhaj UP. 2020. Microbial study of organisms isolated from nutritional fruit juices surrounded by local fruit market in Nanded, Maharashtra, India. Biodiversitas 21: 4240-4246. Growing populations depend on the various food products for their nutritional values, but the hidden hunger for nutrients and vitamins are fulfilled by the fruits and their juices. Fruit juices are one of the most dependable food products, available in the Indian markets. But, standard and quality of juices always matters, hence most suggested one are freshly prepared juices as processed and packed one are mostly contains artificial flavors and food additives in the form of preservatives. Even these freshly prepared juices by local staler are also not free from contaminations. Hence, in this study we focus on the quality of fruit juices sold by the local seller. For this purpose, we collected 5 samples of fruit juices from the premises of Swami Ramanand Teerth Marathwada University, Nanded. The fruit juices are on orange, chiku, banana, apple and grapes. These samples were maintained in the basal media nutrient broth and uniculture of bacteria were isolated and maintained in the slant agar for further experiments. Total microbial load was calculated from collected and it was found that these juice samples contain significant bacterial load (2.5 x 106cfu/mL) that can cause diseases. In further investigations and identifications through the biochemical tests, we found that these juices contaminated with coliforms like E. coli and Klebsiella, along with this we also detected the presence of Listeria spp and Staphylococcus in juices samples.

Microbiological Quality and Prevalence of β-Lactam Antibiotic Resistance Genes in Oysters (Crassostrea rhizophorae)

2017 ◽  
Vol 80 (3) ◽  
pp. 488-496 ◽  
Author(s):  
Maria Aparecida da RessurreiÇão Brandão ◽  
Amanda Teixeira Sampaio Lopes ◽  
Maria Tereza da Silva Neta ◽  
Rhyan Barros Farias de Oliveira ◽  
Rachel Passos Rezende ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Antibiotic Resistance Genes ◽  
Human Consumption ◽  
E Coli ◽  
Lactam Antibiotic ◽  
Crassostrea Rhizophorae

ABSTRACTThe microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA. Our results detected total coliforms, Escherichia coli, and enterobacteria in the oysters from urban estuaries, which is indicative of poor water quality. In addition, our detection of the eaeA and stxA2 virulence genes in 16.7% of E. coli isolates from oysters from this region suggests the presence of multiantibiotic-resistant enteropathogenic and enterohemorrhagic E. coli strains. During periods of low precipitation, increased contamination by E. coli (in winter) and Vibrio parahaemolyticus (in autumn) was observed. In contrast, cultivated oysters inhabiting monitored farms in preserved areas had low levels of bacterial contamination, emphasizing that oyster culture monitoring enhances food quality and makes oysters fit for human consumption. Distinct antibiotic resistance profiles were observed in bacteria isolated from oysters collected from different areas, including resistance to β-lactam antibiotics. The presence of the blaTEM gene in 91.3% of oyster samples indicated that microorganisms in estuarine water conferred the capability to produce β-lactamase. To our knowledge, this is the first study to directly quantify and detect β-lactam antibiotic resistance genes in oysters. We believe our study provides baseline data for bacterial dynamics in estuarine oysters; such knowledge contributes to developing risk assessments to determine the associated hazards and consequences of consuming oysters from aquatic environments containing pathogenic bacteria that may possess antibiotic resistance genes.

scholarly journals Microbial contamination in industrial tofu

2017 ◽  
Vol 47 (3) ◽  
Author(s):  
Thaís Teresa Brandão Cavalheiro Ribeiro ◽  
Géssica Costa ◽  
Marisa da Costa
Keyword(s):  
Bacillus Cereus ◽  
Microbial Contamination ◽  
Microbiological Quality ◽  
Human Consumption ◽  
Microbial Quality ◽  
Porto Alegre ◽  
E Coli ◽  
Food Borne ◽  
Food Borne Illness

ABSTRACT: This study aimed to evaluate the microbiological quality of tofu sold in supermarkets in Porto Alegre/Brazil. Bacteria counts were performed for Bacillus cereus , mesophilic, coliforms and Staphylococcus coagulase positive and negative. The presence of Listeria sp. was also evaluated. Two different brands of tofu (A and B) were collected, one lot per month, for six months. Five samples from each lot were analyzed. All lots presented mesophilic aerobic counts above 4.3x105CFU g-1. Four of the six lots from brand A and all lots from brand B showed E. coli and/or Staphylococcus coagulase positive counts above the Brazilian law accepted limits. The Staphylococcus coagulase negative counts were higher than those of coagulase positive in all lots. In all lots where Staphylococcus coagulase positive counts were above the legal limit, there were counts of coagulase negative above 104CFU g-1. B. cereus and Listeria sp. were not found in either brand. The majority of lots of brand A and all lots of brand B were unsuitable for human consumption. Our results showed that there are problems in tofu manufacturing in both industries analyzed. There is a need of improvement on its microbial quality to avoid problems of food-borne illness, and finally the need of a better control by the Brazilian inspection services.

scholarly journals Microbiological Quality of Street Vended Drinking Water in Dhaka City and Screening for Antibiotics Resistance of Isolated Salmonella spp and Pseudomonas spp.

2014 ◽  
Vol 6 (2) ◽  
pp. 359-371
Author(s):  
S. Ahmed ◽  
K. R. Mahbub ◽  
M. M. Ahmed ◽  
M. Rahman ◽  
M. M. Hoque
Keyword(s):  
Drug Resistance ◽  
Drinking Water ◽  
Water Samples ◽  
Biochemical Characterization ◽  
Microbiological Quality ◽  
Antibiotics Resistance ◽  
Human Consumption ◽  
Dhaka City ◽  
Street Vendors

The present study was aimed to assess the microbiological quality of street vended drinking water of Dhaka city. The water samples were collected from street vendors in different areas of Dhaka city. All of the 30 samples were found having microorganisms higher than WHO limits for drinking water. Four (13.34%) samples were confirmed to have Salmonella contamination and twenty (66.67%) samples were contaminated with Pseudomonas. Based on morphological and biochemical characterization Salmonella isolates were identified as Salmonella choleraesuis and Salmonella bongori. Among the isolates of Pseudomonas, fourteen were identified as Pseudomonas alcaligens and six were as Pseudomonas aeruginosa. During antibiogram for Salmonella, 100% of the isolates were found resistant to Penicillin. Chloramphenicol, doxycycline, Gentamycin, Neomycin were sensitive to all of the isolates. The Pseudomonas isolates showed a significant drug resistance to Penicillin (100%), Ampicillin (95%), Amoxicillin (95%) and Nalidixic acid (85%). The present study demonstrates that drinking water samples from street vendors in Dhaka city are not complying with microbiological specifications of WHO and indicates that street vending drinking water in Dhaka city may not be safe for human consumption and also shows that these are the potential sources of drug resistance Salmonella and Pseudomonas.  Keywords: Street vended drinking water; Antibiotic resistance; Salmonella; Pseudomonas. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i2.17640 J. Sci. Res. 6 (2), 359-371 (2014)

scholarly journals Microbiological Profiling of Ice Cream Samples Sold in the Retail Shops in Dhaka City

2016 ◽  
pp. 45-48
Author(s):  
Tasmina Rahman ◽  
Bashudeb Chandra Sharma ◽  
Md Niazul Isalm
Keyword(s):  
Bacterial Load ◽  
Age Groups ◽  
Microbiological Quality ◽  
Ice Cream ◽  
Dhaka City ◽  
Shigella Spp ◽  
Biochemical Identification ◽  
Ice Creams ◽  
And Storage

Being a popular ready-to-eat food among all age groups, ice creams warrant the maintenance of sound microbiological quality for the sake of public health safety. Present study investigated the microbiological quality of the commonly consumed ice creams available within the retail shops in Dhaka city, Bangladesh. Five ice creams from each comprising 3 types of samples (soft, hard & flavored) of 7 most popular brands (a total of 90 samples) were collected and assessed for the presence of contaminating bacteria through conventional microbiological and biochemical identification methods. Total viable bacterial load was estimated within a range of 4.9 × 103 to 7.9 × 105 cfu/ ml. Brand A ice cream samples were noticed to harbor the least amount of microorganisms. On the other hand 65% of samples belonging to brands B and C were found to be contaminated with Shigella spp. while 30% of samples of brand B and C and 60% of brand E were contaminated with L. monocytogenes. While some were found to be microbiologically sound, most of the ice cream samples studied were found to be contaminated with microorganisms. Maintenance of appropriate hygienic condition during manufacturing and storage of ice cream products are thus recommended to ensure the consumer health safety.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 45-48

scholarly journals Microbiological Quality of Local Market Vended Freshly Squeezed Fruit Juices in Dhaka City, Bangladesh

1970 ◽  
Vol 44 (4) ◽  
pp. 421-424 ◽  
Author(s):  
M Shakir Uddin Ahmed ◽  
Tania Nasreen ◽  
Badrunnessa Feroza ◽  
Sahana Parveen
Keyword(s):  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Fruit Juices ◽  
Local Market ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
E Coli

Freshly squeezed or freshly prepared fruit juices sold by local market vendors in Dhaka city were analyzed for the microbiological quality. Total viable bacterial counts, fungal counts, total coliform, faecal coliform and the presence of pathogenic microorganisms such as E. coli, Bacillus cereus, Staphylococcus aureus, Salmonella, Streptococcus were analyzed by standard methods. The total viable count of samples ranged from 3.00×102 to 9.60×108 and fungal counts ranged from 1.00×102 to 8.05×104. Out of 114 freshly prepared fruit juices samples collected 113 samples (99%) showed the presence of coliform and E. coli. The other bacteria like B. cereus, Staphylococcus aureus, Salmonella, Streptococcus were found in 64.91%, 6.14%, 7.89% and (5.26%) of the tested samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. It was concluded that due to unhygienic fruit handling in the unsanitary environmental conditions under which the vendors operate the juices become contaminated with harmful bacteria. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. Key Words: Fruit juices; Microorganisms; Contamination; E. coli; Microbiological quality standards.   DOI: 10.3329/bjsir.v44i4.4591 Bangladesh J. Sci. Ind. Res. 44(4), 421-424, 2009

scholarly journals Análise microbiológica da água utilizada para consumo nas escolas de Esperança, Paraíba

2017 ◽  
Vol 1 (37) ◽  
pp. 11 ◽  
Author(s):  
Aldeni Barbosa Da Silva ◽  
Janaina Moreira De Brito ◽  
Jonatas Da Silva Duarte ◽  
Olga Elyzabeth Lucena Almeida
Keyword(s):  
Public Schools ◽  
Microbiological Quality ◽  
Human Consumption ◽  
State University ◽  
Total Coliforms ◽  
E Coli ◽  
Wide Mouth ◽  
Yellow Coloration ◽  
C 30

This project had the objective of verifying the microbiological quality of the water that supplies seven public schools in the urban area of the municipality of Esperança-PB. Initially, the samples were collected in wide - mouth glass bottles, protected with laminated paper, pre-sterilized (121º C - 30’ in autoclave), and were sent to the Environmental Sanitation Laboratory of the State University of Paraíba - Campina Grande Campus, where the qualitative analysis of Total Coliforms and Escherichia coli were performed using the Colilert Enzymatic Chromogenic Substrate method. Soon after the application of the Colilert reagent and incubation for 24 hours at 37 ° C, a yellow coloration was observed in samples 6 and 7, highlighting the presence of total coliforms. However, the presence of E. coli was not detected in any of the analyzed samples, since they did not show fluorescence under ultraviolet light. This concludes that the waters of samples 6 and 7 are inappropriate for human consumption. According to Ordinance No. 2.914 / 2011 of the Ministry of Health, when samples with positive results are detected for total coliforms, even in presumptive tests, corrective actions must be taken and new samples must be collected on successive days until satisfactory results are obtained.

A Survey of the Microbiological Quality of Kangaroo Carcasses Processed for Human Consumption in Two Processing Plants in Queensland, Australia

2007 ◽  
Vol 70 (5) ◽  
pp. 1249-1251 ◽  
Author(s):  
SOFRONI EGLEZOS ◽  
BIXING HUANG ◽  
ED STUTTARD
Keyword(s):  
Significant Relationship ◽  
Microbiological Quality ◽  
Plate Count ◽  
Human Consumption ◽  
E Coli ◽  
Beef Carcasses ◽  
Processing Plants ◽  
Aerobic Plate Count ◽  
Whole Muscle

An investigation of the microbiological quality of kangaroo carcasses at two Queensland processing plants was carried out. A total of 836 whole muscle samples were taken, 801 from plant A and 35 from plant B. Samples were analyzed for aerobic bacteria, Escherichia coli, and Salmonella. The mean adjusted aerobic plate count (APC) was 2.8 log CFU/g, and counts at the 90th, 95th, and 99th percentiles were 4.2, 4.9, and 6.4 log CFU/g, respectively. The maximum number of bacteria recovered was 6.5 log CFU/g. E. coli was detected in 13.9% of samples, for which the adjusted mean was 0.7 log CFU/g, and counts at the 90th, 95th, and 99th percentiles were 1.4, 2.0, and 3.0 log CFU/g, respectively. Salmonella was detected in 0.84% of samples. There was no significant relationship (P &lt; 0.05) between season and APC or E. coli count. There was a significant relationship (P &lt; 0.001) between Salmonella prevalence and summer. The microbiological quality of Queensland kangaroo carcasses is similar to that obtained during other excision-based studies of kangaroo, wild boar, and beef carcasses.

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