A Highly Sensitive Fluorescence Immunoassay for the Determination of Diethylstilbestrol
2013 ◽
Vol 295-298
◽
pp. 622-625
Keyword(s):
A highly sensitive direct competitive fluorescence immunoassay (FIA) was developed for the determination of diethylstilbestrol (DES), based on the antibody coating format using biotin-streptavidin amplification system. In the assay, DES competed with biotinylated goat anti-rabbit IgG for the immobilized antibody, then the streptavidin-FITC was added to link to the biotinylated goat anti-rabbit IgG as a tracer. Under optimal conditions, the assay provided a wide analytical range of 0.001-10000 ng/mL with a detection limit of 2.91×10-4ng/mL (R2=0.9943). This method was then applied to the determination of DES in chicken with satisfactory results