Characteristics of Biohydrogen Production by Mixed Culture Using Bagasse as the Substrate

2010 ◽  
Vol 113-116 ◽  
pp. 1749-1754
Author(s):  
An Ying Jiao ◽  
Yong Feng Li ◽  
Bing Liu ◽  
Kun Liu
Keyword(s):  
Gas Chromatography ◽  
Mixed Culture ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Hydrogen Yield ◽  
Batch Experiments ◽  
Ethanol Acetic Acid ◽  
Liquid Products ◽  
Initial Ph ◽  
Favorable Conditions

Batch culture of dark fermentation was carried out to study the feasibility of biohydrogen production using bagasse as the substrate. In dark fermentation, hydrogen was produced by mixed culture using bagasse as the substrate. To establish favorable conditions for maximum hydrogen production, process parameters such as temperature and initial pH of the medium were investigated. Also, the component of biogas and liquid products of effluent by fermentation were analyzed by gas chromatography. The VFAs were mostly ethanol, acetic acid, propionic acid and butyric acid, and no valeric acid was observed. It is demonstrated that the hydrogen yield reached the maximum of 30.5mlH2/g bagasse while the temperature was 35°C in batch experiments under a series of temperature (25, 30, 35, 40°C) conditions. The initial pH ranged from 6.8 to 8.5, and the yield of hydrogen reached the maximum of 32mlH2/g bagasse with the initial pH of 8.5.

scholarly journals Thermophilic Dark Fermentation of Sewage Sludge for Biohydrogen Production - Influence of pH

2018 ◽  
Vol 20 (3) ◽  
pp. 564-571
Keyword(s):  
Sewage Sludge ◽  
Hydrogen Production ◽  
Waste Water Treatment ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Pure Hydrogen ◽  
Constant Ph ◽  
Initial Ph ◽  
Sludge Waste ◽  
Almost All

<p>This study investigates the usability of sewage sludge, waste from a waste water treatment facility, at the stable thermophilic temperature and different pH conditions in the biohydrogen production by dark fermentation. Without the addition of a pure hydrogen producer and nutrient source, the effect of a different constant pH in the range of pH 4-9 on biohydrogen production using sewage sludge was compared with that of a different initial pH. It was understood from the results that biohydrogen production varies according to the characterization of sewage sludge. In the experiments, the lag time was insignificant (~2h). The maximum hydrogen production was achieved at pH 5 within the first 24-30 hours of fermentation (92894 mL m-3 H2). Therefore, it was determined that the higher digestion efficiencies of the sewage sludge were obtained at pH 5. In general, with the increase in methanogens in the medium, the hydrogen producing ability and hydrogen content of the sewage sludge gradually decreased. Hydrogen production at almost all the pH values after the third day was less than 1000 mL m-3.</p>

scholarly journals PRODUKSI BIOHIDROGEN DARI HIDROLISAT AMPAS TAHU SECARA FERMENTASI ANAEROB MENGGUNAKAN KULTUR CAMPURAN

REAKTOR ◽  
2014 ◽  
Vol 15 (2) ◽  
pp. 87
Author(s):  
Amir Husin ◽  
Sarto Sarto ◽  
Siti Syamsiah ◽  
Imam Prasetyo
Keyword(s):  
Solid Waste ◽  
Reducing Sugar ◽  
Hydrogen Yield ◽  
Batch Experiments ◽  
Two Stage ◽  
Second Stage ◽  
One Stage ◽  
Initial Ph ◽  
Two Stages ◽  
By Products

Ampas tahu merupakan produk samping industri pengolahan tahu berbahan dasar kacang kedelai (Glysine max.).  Dalam studi ini, ampas tahu yang telah mengalami perlakuan-awal dengan metode yang berbeda dikonversi menjadi hidrogen menggunakan kultur campuran.  Pengaruh perlakuan-awal asam satu-tahap (0,5% dan 10% berat HCl) dan dua-tahap (tahap I 0,5%  dan tahap II 10% berat HCl) terhadap sakarifikasi ampas tahu juga diinvestigasi.  Lebih lanjut, pengaruh perlakuan awal asam satu-tahap maupun dua-tahap terhadap produksi hidrogen dan efisiensi degradasi gula tereduksi dibandingkan.  Yield total gula-tereduksi 503,73 mg/g ampas tahu diperoleh dari perlakuan-awal asam dua-tahap.  Nilai ini kurang lebih 4 kali lebih tinggi dibanding hasil dari perlakuan-awal asam satu-tahap menggunakan 0,5% berat HCl. Studi produksi biohidrogen dilakukan secara batch menggunakan kultur campuran dengan kondisi reaksi  35oC dan pH awal 6,5,  Hasil percobaan menunjukkan, bahwa yield hidrogen kumulatif  maksimum 0,928  mmol/g  ampas tahu diperoleh dari perlakuan  asam dua-tahap  atau meningkat 1,8 kali dibanding perlakuan satu-tahap menggunakan 0,5% berat HCl. Key words : ampas tahu,  perlakuan asam, produksi hidrogen Abstract Tofu solid waste is one of the by-products of tofu-processing industry.  In this study, batch experiments were carried out to convert tofu solid waste pretreated by different methods into hydrogen using mixed culture.  The effects of one-stage (0.5% or 10% HCl) and two-stage (first stage 0.5% HCl) and second stage 10% HCl) pretreatments on the saccharification of tofu solid waste were also studied.  Furthermore, the effects of  and/or  two- stages acid pretreatments on hydrogen production and degradation efficiencies the reducing-sugar (RS) were compared.  A maximum total RS yield of 503.73 mg/g-tofu solid waste was obtained from substrate pretreated with two-stages method.  It was approximately 4-fold greater than that from substrate pretreated with one-stage method using 0.5% wt HCl. At the reaction condition of 35oC, initial pH =  6.5, and RS concentration based on 2 grams of tofu solid waste pretreated, a maximum cumulative hydrogen yield was 0.928  mmol/g-tofu solid waste from substrate  pretreated  with  two-stages method.  It was approximately 1.8-fold greater than that from substrate pretreated with one-stage method using 0.5% wt HCl. The results show that two-stage acid preteatment can enhancing the amount of  reducing sugar in the mixture and hydrogen yield from tofu solid wastes. 

Effect of pH control strategies and substrate concentration on the hydrogen yield from fermentative hydrogen production in large laboratory-scale

2012 ◽  
Vol 65 (2) ◽  
pp. 262-269 ◽  
Author(s):  
I. Mariakakis ◽  
J. Krampe ◽  
H. Steinmetz
Keyword(s):  
Hydrogen Production ◽  
Control Strategies ◽  
Sucrose Concentration ◽  
Ph Control ◽  
Laboratory Scale ◽  
Hydrogen Yield ◽  
Batch Experiments ◽  
Ph Adjustment ◽  
Fermentative Hydrogen Production ◽  
Initial Ph

A series of batch experiments investigating two different pH control strategies, initial pH adjustment and continuous pH control, have been carried out in large laboratory-scale reactors with working volumes of 30 L. In both cases, pH was varied between 5 and 7.5. Sucrose concentrations were also varied starting from 0 up to 30 g/L. Higher hydrogen production yields can be achieved by batch experiments through continuous pH control than by simple initial pH adjustment. In the case of continuous pH control, maximization of hydrogen yield was acquired for slightly acidic pH of 6.5. Continuous pH control in the neutral pH range of 7.0 and in pH lower than 6.5, induced a reduction in the hydrogen production yield. Sucrose can be completely degraded only for a pH higher than 6. Lower pH values seem to inhibit the hydrogen-producing bacteria. Under the conditions of continuous pH adjustment at pH 6.5 and a sucrose concentration of 25 g/L the maximum hydrogen yield of 1.79 mol H2/mol hexose was obtained. These conditions could be applied for the batch start-up of large fermentors.

scholarly journals Biohydrogen Production and Kinetic Modeling Using Sediment Microorganisms of Pichavaram Mangroves, India

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
P. Mullai ◽  
Eldon R. Rene ◽  
K. Sridevi
Keyword(s):  
Trace Metal ◽  
Ferrous Sulphate ◽  
Model Fitting ◽  
Biohydrogen Production ◽  
Hydrogen Yield ◽  
Influence Of Process Parameters ◽  
Initial Substrate ◽  
Initial Ph ◽  
Initial Medium ◽  
Good Agreement

Mangrove sediments host rich assemblages of microorganisms, predominantly mixed bacterial cultures, which can be efficiently used for biohydrogen production through anaerobic dark fermentation. The influence of process parameters such as effect of initial glucose concentration, initial medium pH, and trace metal (Fe2+) concentration was investigated in this study. A maximum hydrogen yield of 2.34, 2.3, and 2.6 mol H2mol−1glucose, respectively, was obtained under the following set of optimal conditions: initial substrate concentration—10,000 mg L−1, initial pH—6.0, and ferrous sulphate concentration—100 mg L−1, respectively. The addition of trace metal to the medium (100 mg L−1FeSO4·7H2O) enhanced the biohydrogen yield from 2.3 mol H2 mol−1glucose to 2.6 mol H2mol−1glucose. Furthermore, the experimental data was subjected to kinetic analysis and the kinetic constants were estimated with the help of well-known kinetic models available in the literature, namely, Monod model, logistic model and Luedeking-Piret model. The model fitting was found to be in good agreement with the experimental observations, for all the models, with regression coefficient values >0.92.

scholarly journals BIOHYDROGEN PRODUCTION FROM WASTES OF PLANT AND ANIMAL ORIGIN VIA DARK FERMENTATION

2019 ◽  
Vol 27 (2) ◽  
pp. 101-113 ◽  
Author(s):  
Weronika Cieciura-Włoch ◽  
Sebastian Borowski
Keyword(s):  
Hydrogen Production ◽  
Sugar Beet ◽  
Dark Fermentation ◽  
Biohydrogen Production ◽  
Animal Origin ◽  
Hydrogen Yield ◽  
Vegetable Waste ◽  
Fruit And Vegetable ◽  
Kitchen Waste ◽  
High Hydrogen

This study investigated the batch experiments on biohydrogen production from wastes of plant and animal origin. Several substrates including sugar beet pulp (SBP), sugar beet leaves (SBL), sugar beet stillage (SBS), rye stillage (RS), maize silage (MS), fruit and vegetable waste (FVW), kitchen waste (KW) and slaughterhouse waste (SHW) including intestinal wastes, meat tissue, post flotation sludge were tested for their suitability for hydrogen production. Generally, the substrates of plant origin were found to be appropriate for dark fermentation, and the highest hydrogen yield of 280 dm3 H2/kg VS was obtained from fruit and vegetable waste. Contrary to these findings, slaughterhouse waste as well as kitchen waste turned out to be unsuitable for hydrogen production although their methane potential was high. It was also concluded that the combined thermal pretreatment with substrate acidification was needed to achieve high hydrogen yields from wastes.

scholarly journals Biohydrogen production by fermentive bacterium Clostridium sp. Tr2 using batch fermenter system controlled pH under dark fermentation

2018 ◽  
Vol 9 (1) ◽  
pp. 4-10
Author(s):  
Thi Thu Huyen Nguyen ◽  
Thi Yen Dang ◽  
Thuy Hien Lai
Keyword(s):  
Hydrogen Production ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
Dark Fermentation ◽  
Gas Production ◽  
Biohydrogen Production ◽  
Great Promise ◽  
Hydrogen Yield ◽  
Promising Alternative ◽  
Fermentative Bacteria

Limitation of fuels reserves and contribution of fossil fuels to the greenhouse effect leads to develop anew, clean and sustainable energy. Among the various options, biohydrogen appears as a promising alternative energy source. The fermentative hydrogen production process holds a great promise for commercial processes. Hydrogen production by fermentative bacteria is a very complex and greatly influenced by pH. This paper presents biohydrogen production by bacterial strain Clostridium sp. Tr2. Operational pH strongly affected its hyrogen production. Its gas production rate as well as obtained gas product were roughly increase twice under controlled pH at 6 than non-controlled condition. Dark fermentation for hydrogen production of strain Tr2 was performed under bottle as well as automatic fermenter scale under optimal nutritional and environmental conditions at 30°C, initial pH at 6.5, then pH was controlled at 6 for bioreactor scale (BioFlo 110). Bioreactor scale was much better for hydrogen production of strain Tr2. Clostridium sp. Tr2 produced 0.74 L hydro (L medium)-1 occupying 72.6 % of total gas under bottle scale while it produced 2.94 L hydro (L medium)-1 occupying 95.82 % of total gas under fermenter scale. Its maximum obtained hydrogen yield of Clostridium sp. Tr2 under bioreactor scale Bioflo 110 in optimal medium with controlled pH 6 was 2.31 mol hydro (mol glucose)-1. Dự trữ nhiên liệu có giới hạn và việc sử dụng nhiên liêu hoá thạch góp phần không nhỏ gây hiệu ứng nhà kính dẫn đến cần phải phát triển năng lượng mới, sạch và bền vững. Trong số các giải pháp, hydro sinh học xuất hiện như một nguồn năng lượng thay thế đầy hứa hẹn. Quá trình lên men sản xuất hydro có tiềm năng lớn để áp dụng trong sản xuất thương mại. Tuy nhiên qúa trình này rất phức tạp và chịu ảnh hưởng lớn bởi pH. Nghiên cứu này trình bày sản xuất hydro sinh học do chủng vi khuẩn Clostridium sp. Tr2. Quá trình sản xuất hydro của chủng này bị ảnh hưởng mạnh mẽ bởi pH thay đổi trong quá trình lên men. Tốc độ tạo khí cũng như lượng khí thu được của chủng này tăng gần gấp đôi trong môi trường có duy trì pH ở pH 6 so với môi trường không kiểm soát pH. Quá trình lên men tối sản xuất hydro của chủng Tr2 được thực hiện ở quy mô bình thí nghiệm cũng như bình lên men tự động trong điều kiện môi trường tối ưu ở 30°C, pH ban đầu 6.5, ở qui mô bình lên men tự động (BioFlo 110), pH môi trường sau đó được duy trì ổn định ở pH 6. Lên men sản xuất hdyro của chủng Tr2 trong bình lên men tự động tốt hơn rất nhiều so với lên men trong bình thí nghiệm. Clostridium sp. Tr2 chỉ tạo ra được 0,74 L hydro (L medium)-1 chiếm 72,6 % tổng thể tích khí thu được ở điều kiện lên men bình thí nghiệm trong khi chủng này sản xuất được 2,94 L hydro (L medium)-1 chiếm 95,82 % tổng thể tích khí ở điều kiện lên men tự động. Sản lượng hydro thu được lớn nhất của chủng này trong bình lên men tự động BioFlo 110 trong trong môi trường tối ưu có kiểm soát pH tại pH 6 là 2,31 mol hydro (mol glucose)-1.

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