Preparation of Alginate/Silica Composite Beads with In Vitro Apatite-Forming Ability

2011 ◽  
Vol 236-238 ◽  
pp. 1889-1892 ◽  
Author(s):  
Fan Xiao ◽  
Fei Gao ◽  
Zhi Xian Zhang ◽  
Wei Li
Keyword(s):  
Biomedical Applications ◽  
Sol Gel ◽  
Calcium Nitrate ◽  
Phosphate Solution ◽  
X Ray Diffraction ◽  
Silica Composite ◽  
Composite Beads ◽  
Ft Ir ◽  
Diffraction Peaks

Alginate/silica composite beads of mm-size for biomedical applications were prepared from water glass and sodium alginate via a simple sol-gel route. They kept their original shapes when soaked in the phosphate solution of lower pH. In this case, faint calcium phosphate peaks were observed in X-ray diffraction. Peaks corresponding to phosphate group were also found in FT-IR. Apatite was obtained on the surface of beads made with calcium chloride after soaking in phosphate solution while monetite was formed on the surface of beads made with calcium nitrate.

Influence of Li2+ Doping on the Structural and Optical Properties of CaO Synthesized by Sol–Gel Process

2019 ◽  
Vol 07 (01n02) ◽  
pp. 1950002
Author(s):  
Nadir Lalou ◽  
Ahmed Kadari
Keyword(s):  
Optical Properties ◽  
Band Gap ◽  
Sol Gel ◽  
Calcium Nitrate ◽  
Lithium Nitrate ◽  
X Ray Diffraction ◽  
Structural And Optical Properties ◽  
Sol Gel Process ◽  
Ft Ir ◽  
First Time

This work proposes the synthesis of nanocrystalline calcium oxide (CaO) pure and doped with different concentrations of lithium (Li[Formula: see text]) ions by sol–gel process. Calcium nitrate (Ca(NO[Formula: see text]4H2O; 99.99%) and lithium nitrate (LiNO3; 99.99%) were used as precursors. The synthesized powders were characterized by several techniques such as: UV-Vis transmission spectroscopy, Fourier Transform Infra-red spectroscopy (FT-IR) and X-ray diffraction (XRD). The main objective of this paper is to study the influence of lithium (Li[Formula: see text] ratio) on the structural and optical properties of synthesized powders. The band gap values decreased with the increasing of Li[Formula: see text] ions in CaO lattice; the slight change in the band gap was directly related to the energy transfer between the CaO excited states and the 2s levels of Li[Formula: see text] ions. The influence of Li[Formula: see text] doping on the physical properties of CaO nanocrystalline will be studied for the first time in this work; no literature has previously published this kind of impurities.

Apatite Formation on PDMS-Modified SiO2-CaO-P2O5 Hybrids Prepared with Different P2O5 Content by Sol-Gel Method

2004 ◽  
Vol 449-452 ◽  
pp. 1121-1124 ◽  
Author(s):  
Do Won Seo ◽  
J.G. Kim ◽  
Yun Hae Kim ◽  
Chin Myung Whang
Keyword(s):  
Sol Gel ◽  
Calcium Nitrate ◽  
Apatite Formation ◽  
Calcium Nitrate Tetrahydrate ◽  
X Ray Diffraction ◽  
Phosphate Ions ◽  
P2o5 Content ◽  
Sol Gel Process ◽  
Nitrate Tetrahydrate

Bioactive ORMOSILS (organically modified silicate), PDMS-CaO-SiO2-P2O5 with five different P2O5 content (0, 0.01, 0.03, 0.06, 0.09 mol%) have successfully been synthesized by sol-gel process. The hybrids have been prepared with polydimethylsiloxane (PDMS), tetraethoxysilane (TEOS), calcium nitrate tetrahydrate [Ca(NO3)2 4H2O] and triethyl phosphate (TEP) as starting materials and subsequently soaked into the simulated body fluid (SBF) for different period of time and the bioactivity of hybrids was determined by examining the apatite formation on the surface of the specimen by FT-IR, Thin-Film X-ray Diffraction, and Scanning Electron Microscopy (SEM). All of the prepared samples with different P2O5 content showed in vitro bioactivity. It was observed that the increase in P2O5 content up to 0.03 mole % increases the apatite formation compared to P2O5- free hybrids. However, further increase in P2O5 concentration slows down the formation of the apatite layer most probably due to the decrease of pH of SBF by dissolution of a large amount of phosphate ions.

Synthesis, Characterization and Bioactivity Studies of Calcium Silicate Bioceramics

2012 ◽  
Vol 584 ◽  
pp. 479-483 ◽  
Author(s):  
R. Lakshmi ◽  
Swamiappan Sasikumar
Keyword(s):  
Sol Gel ◽  
Combustion Process ◽  
Calcium Nitrate ◽  
Secondary Phase ◽  
X Ray Diffraction ◽  
Xrd Pattern ◽  
Sem Image ◽  
Gel Combustion ◽  
Ft Ir ◽  
Highly Porous

Wollastonite is one of the most widely used bioceramic due to its biocompatibility and bioactivity. Wollastonite (CaSiO3) was synthesized by sol-gel combustion process using citric acid as a fuel/reductant and nitrate as an oxidant. Calcium nitrate was taken as a source of calcium and tetraethyl orthosilicate was taken as the source of silicate. The obtained product was characterized by powder X-ray diffraction, Fourier - Transform Infrared spectroscopy and Scanning electron microscopy. The XRD pattern shows that the product formed is a pure and single phasic wollastonite. The FT-IR spectra revealed that there is no secondary phase present in the product. The SEM image shows that the product is highly porous. The particle size calculated using Scheerer’s formula shows that the particles are in a nano regime.

Preparation of Silica Microspheres Coated by Nano-ZrO2

2014 ◽  
Vol 924 ◽  
pp. 36-40
Author(s):  
Chun Xiao Yue ◽  
Peng Ding ◽  
Yu Zhao ◽  
Li Yi Shi
Keyword(s):  
Sol Gel ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Silica Microspheres ◽  
Surface Layers ◽  
Sem Image ◽  
Sol Gel Process ◽  
Stretching Vibration ◽  
Ft Ir ◽  
Diffraction Peaks

Silica microspheres coated by nanoZrO2 were prepared by the sol-gel process using butoxidesolution (TBOZ) as precursor. The nanomicrospheres were formed via hydrolysis and condension reaction of metal alkoxides with the hydroxyl (-OH) of silica microspheres by form Zr-O-Si bonds. The samples were characterized by X-ray diffraction, FT-IR and SEM. Presence of the FT-IR bands with frequencies ranging from 800 to 900 cm-1 can be assigned to SiO vibration, and to ZrOSi stretching vibration. XRD analysis shows obviously the characteristic diffraction peaks of tetragonal (T-ZrO2) in the sample by heat-treatment at 400°C. The SEM image showed that the surface layers of silica microspheres were coated by nanoparticles.

Synthesis and Characterization of Sol-Gel Derived Hydroxyapatite-Bioglass Composite Nanopowders for Biomedical Applications

2012 ◽  
Vol 12 ◽  
pp. 51-57 ◽  
Author(s):  
S. Adibnia ◽  
Ali Nemati ◽  
Mohammad Hosseien Fathi ◽  
S. Baghshahi
Keyword(s):  
Electron Microscopy ◽  
Inductively Coupled Plasma ◽  
Biomedical Applications ◽  
Sol Gel ◽  
In Vitro Study ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Inductively Coupled ◽  
Transmission Electron

The main purpose of this study is to prepare and characterize hydroxyapatite (HA)–10%wt bioglass (BG) composite nanopowders and its bioactivity. Composites of hydroxyapatite with synthesized bioglass are prepared at various temperatures. Suitable calcination temperature is chosen by evaluating of the phase composition. X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) techniques are utilized to characterize the prepared nanopowders. The bioactivity of the prepared composite samples is evaluated in an in vitro study by immersion of samples in simulated body fluid (SBF) for predicted time. Fourier transformed infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) are used for evaluation of apatite formation and the bioactivity properties. Results show that HA-BG composite nanopowders are successfully prepared without any decomposition of hydroxyapatite. The suitable temperature for calcination is 600°C and the particle size of hydroxyapatite is about 40-70 nm. The apatite phase forms after 14 days immersing of the samples in SBF. It could be concluded that this process can be used to synthesize HA-BG composite nanopowders with improved bioactivity which is much needed for hard tissue repair and biomedical applications.

In Vitro Bioactivity Study on ZnI2-HAp Nanoparticles

2020 ◽  
Vol 15 (4) ◽  
pp. 523-531
Author(s):  
Mohammad Y. Alshahrani ◽  
H. Algarni ◽  
Ibrahim AlShahrani ◽  
I. S. Yahia ◽  
H. Y. Zahran ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Sol Gel ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Mouse Splenocytes ◽  
Fourier Transform Raman Spectroscopy ◽  
Sol Gel Process ◽  
Splenic Cells ◽  
Ft Ir

The manufactured powder of immaculate HAp which is doped with 1.0, 5.0, 10, 20, 30, and 40 in wt.% ZnI2 has been prepared via the sol–gel process. The characterization of samples was achieved by X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR) and Fourier Transform Raman spectroscopy (FT- Raman). The morphology of the obtained HAp powder was studied through SEM analysis. The investigation proves that all prepared samples have the dimension of a nanoscale. The prepared powders were examined for its anti-growth, stimulatory/cytotoxic potentials on normal/activated mouse splenocytes in vitro as well as the lytic effects on red blood cells (RBCs). Results showed that different preparations of ZnI2 had anti-growth effects on normal mouse splenocytes. The pure HAp, 1% and 10% ZnI2 showed no effects on stimulated mouse splenic cells while the other preparations 5%, 20%, 30% and 40% ZnI2 showed inhibitory/cytotoxic effects. All ZnI2 preparations showed safe effects on RBCs.

PREPARATION AND CHARACTERIZATION OF HYDROXYAPATITE-SILICA COMPOSITE NANOPOWDERS

2012 ◽  
Vol 05 ◽  
pp. 630-637 ◽  
Author(s):  
S.M. LATIFI ◽  
M.H. FATHI ◽  
M.A. GOLOZAR
Keyword(s):  
Sol Gel ◽  
Calcium Nitrate ◽  
Bone Substitutes ◽  
Calcium Nitrate Tetrahydrate ◽  
X Ray Diffraction ◽  
X Ray ◽  
Silica Composite ◽  
Biological Responses ◽  
Nanocrystalline Hydroxyapatite ◽  
Nitrate Tetrahydrate

One of the most important objectives in the field of biomaterials science and engineering is development of new materials as bone substitutes. Silica ( SiO 2) has an important role in the biomineralization and biological responses. The aim of this research was to prepare and characterize hydroxyapatite-silica ( HA - SiO 2) composite nanopowder with different content of silica. Hydroxyapatite-silica composite nanopowders with 20 and 40 wt% silica were prepared using a sol–gel method at 600°C with phosphoric pentoxide and calcium nitrate tetrahydrate as a source of hydroxyapatite; also, tetraethylorthosilicate and methyltriethoxisilane as a source of silica. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX) techniques were used for characterization and evaluation of the products. The results indicated the presence of nanocrystalline hydroxyapatite phase beside amorphous silica phase in prepared composite nanopowders. Moreover, by increasing the content of silica in composite nanopowders, the crystallinity will be decreased,and the ability of the product as a bone substitute material might be controlled by changing the content of the ingredients and subsequently its structure.

In Vitro Evaluation of Hydroxyapatite-Containing Glass Coating on Zirconia

2011 ◽  
Vol 493-494 ◽  
pp. 7-10
Author(s):  
Daisuke Yamashita ◽  
Makoto Noda ◽  
Miho Machigashira ◽  
Motoharu Miyamoto ◽  
Hironobu Takeuchi ◽  
...  
Keyword(s):  
Yttria Stabilized Zirconia ◽  
Time Dependent ◽  
Glass Coating ◽  
X Ray Diffraction ◽  
Stabilized Zirconia ◽  
X Ray ◽  
Proliferation And Differentiation ◽  
Ft Ir ◽  
Diffraction Peaks

The aim of this study was to investigate the response of osteoblast-like cells (MC3T3-E1) to the surface of hydroxyapatite-containing glass coating on zirconia (HA-G-Zr) in comparison to yttria stabilized zirconia (Y-TZP). The MC3T3-E1 cells were cultured on HA-G-Zr and Y-TZP specimens in 24-well tissue culture plates. Surface properties were evaluated by X-ray diffractometry, Fourier tranfer infrared microscopy (FT-IR) and scanning electron microscopy (SEM). Cell proliferation in each well was measured by MTT assay. Cell morphology was observed by SEM. Cell differentiation was evaluated by alkaline phosphatase (ALP) activity and osteocalcin levels. After the glass coating on Y-TZP, X-ray diffraction peaks due to hydroxyapatite (HA) were observed clearly. HA-G-Zr appeared on surface of uneven and roughened state, wherein microcracks on the microns in width and many voids of several microns in size were present. Time-dependent proliferation and differentiation of MC3T3-E1 cells were found in all the specimens. MC3T3-E1 cells on HA-G-Zr plates showed higher differentiation than Y-TZP. These results demonstrated that HA-G-Zr showed better cellular biocompatibility than Y-TZP.

A study on using expanded perlite with hydroxyapatite: Reinforced bio-composites

2021 ◽  
pp. 095441192199656
Author(s):  
Erdoğan Karip ◽  
Mehtap Muratoğlu
Keyword(s):  
Body Fluid ◽  
Xrd Analysis ◽  
Cold Isostatic Pressing ◽  
Expanded Perlite ◽  
X Ray Diffraction ◽  
Pressing Method ◽  
X Ray ◽  
Infra Red ◽  
Ft Ir

People are exposed to different kinds of diseases or various accidents in life. Hydroxyapatite (HA) has been widely employed for bone treatment applications. In this study, HA was extracted from sheep bones. Bio-composites were doped with 1, 5, and 10 wt.% of expanded perlite and 5 wt.% of ZrO2–MgO-P2O5. The bio-composites were prepared by the cold isostatic pressing method (250 MPa) and sintered at 900°C for 1 h. In order to evaluate the characteristics of the bio-composites, microhardness, density, X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) analyses were carried out on them. Additionally, the specimens whose characteristics were determined were kept in synthetic body fluid (SBF), and their in vitro behavior was examined. As a result, it was observed that microhardness increased as both the weight and the grain size of the expanded perlite were increased. Calcium silicate, tri-calcium phosphate, and hydroxyapatite were observed in the XRD analysis of all samples, and the formation of apatite structures was increased by addition of ZrO2–MgO–P2O5.

Luminescent Properties of Zn2SiO4:Eu3+,Dy3+ Phosphors via Sol-Gel Process

2012 ◽  
Vol 217-219 ◽  
pp. 733-736
Author(s):  
Xiu Mei Han ◽  
Shu Ai Hao ◽  
Ying Ling Wang ◽  
Gui Fang Sun ◽  
Xi Wei Qi
Keyword(s):  
Thermal Analysis ◽  
Ir Spectra ◽  
Sol Gel ◽  
Luminescent Properties ◽  
Photoluminescence Spectra ◽  
X Ray Diffraction ◽  
X Ray ◽  
Sol Gel Process ◽  
Ft Ir

Zn2SiO4:Eu3+, Dy3+ phosphors have been prepared through the sol-gel process. X-ray diffraction (XRD), thermogravimetric and ddifferential thermal analysis (TG-DTA), FT-IR spectra and photoluminescence spectra were used to characterize the resulting phosphors. The results of XRD indicated that the phosphors crystallized completely at 1000oC. In Zn2SiO4:Eu3+,Dy3+ phosphors, the Eu3+ and Dy3+ show their characteristic red(613nm, 5D0-7F2), blue (481nm, 4F9/2–6H15/2) and yellow (577nm, 4F9/2–6H13/2) emissions.

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