Antibacterial Activity of Silver Nanoparticles Colloidal Sol and its Application in Package Film

2011 ◽  
Vol 380 ◽  
pp. 254-259 ◽  
Author(s):  
Shi Yong Luo ◽  
Juan Chen ◽  
Meng Chen ◽  
Wen Cai Xu ◽  
Xin Lin Zhang
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Fruits And Vegetables ◽  
Silver Content ◽  
Silver Particles ◽  
Pear Fruit ◽  
Film Blowing ◽  
Blend Film ◽  
Nano Size ◽  
Master Batch

Using starch as the stabilizing agent, glucose as reducing agent, nano-size silver particles colloidal sols have been synthesized via ultrasonic irritation in aqueous solution. The nanoparticles size distribution in typical colloidal sol is from 15 to 45 nm. The silver nanoparticles-PE blend film with silver content of 86 ppm was prepared by mixing silver nanoparticles colloids sol with the PE master batch and then by film blowing. The silver nanoparticles-PE blend film and the prepared nano-silver sol show effective antibacteial activity to the Staphylococcus aureus and Escherichia coli. The pear fruit package application of the silver nanoparticles-PE blend film has demonstrated that the film has effective fresh preservation ability for the packaged fruits and vegetables.

scholarly journals Photochemical Synthesis of Silver Hydrosol Stabilized by Carbonate Ions and Study of Its Bactericidal Impact on Escherichia coli: Direct and Indirect Effects

2022 ◽  
Vol 23 (2) ◽  
pp. 949
Author(s):  
Vadim Ershov ◽  
Natalia Tarasova ◽  
Evgeny Abkhalimov ◽  
Alexey Safonov ◽  
Vladimir Sorokin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Photochemical Synthesis ◽  
Bacterial Cells ◽  
Silver Particles ◽  
Photochemical Reduction ◽  
Antiviral Effects ◽  
Spherical Metal Nanoparticles ◽  
20 Nm ◽  
Biological Organisms

The great attention paid to silver nanoparticles is largely related to their antibacterial and antiviral effects and their possible use as efficient biocidal agents. Silver nanoparticles are being widely introduced into various areas of life, including industry, medicine, and agriculture. This leads to their spreading and entering the environment, which generates the potential risk of toxic effect on humans and other biological organisms. Proposed paper describes the preparation of silver hydrosols containing spherical metal nanoparticles by photochemical reduction of Ag+ ions with oxalate ions. In deaerated solutions, this gives ~10 nm particles, while in aerated solutions, ~20 nm particles with inclusion of the oxide Ag2O are obtained. Nanoparticles inhibit the bacterium Escherichia coli and suppress the cell growth at concentrations of ~1 × 10−6–1 × 10−4 mol L−1. Silver particles cause the loss of pili and deformation and destruction of cell membranes. A mechanism of antibacterial action was proposed, taking into account indirect suppressing action of Ag+ ions released upon the oxidative metal dissolution and direct (contact) action of nanoparticles on bacterial cells, resulting in a change in the shape and destruction of the bacteria.

Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

2011 ◽  
Vol 4 (3) ◽  
pp. 1491-1496
Author(s):  
Umadevi M ◽  
Rani T ◽  
Balakrishnan T ◽  
Ramanibai R
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Reduction Method ◽  
Therapeutic Potential ◽  
Chemical Reduction ◽  
Ultrasonic Field ◽  
Great Promise ◽  
Chemical Reduction Method ◽  
E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles. 

Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

2020 ◽  
Vol 21 (11) ◽  
pp. 1129-1137 ◽  
Author(s):  
Somayeh Mirsadeghi ◽  
Masoumeh F. Koudehi ◽  
Hamid R. Rajabi ◽  
Seied M. Pourmortazavi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Plant Extract ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Extraction Temperature ◽  
Silver Particles ◽  
Silver Nps ◽  
Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

scholarly journals Transcriptome analysis of Escherichia coli K1 after therapy with hesperidin conjugated with silver nanoparticles

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Abdulkader Masri ◽  
Naveed Ahmed Khan ◽  
Muhammad Zarul Hanifah Md Zoqratt ◽  
Qasim Ayub ◽  
Ayaz Anwar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Neonatal Meningitis ◽  
E Coli ◽  
Drug Candidates ◽  
Metabolic Genes ◽  
Genetic Mechanisms ◽  
Cell Stress Response

Abstract Backgrounds Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study. Results 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P< 0.01) in both E. coli K1 treated with Hesperidin conjugated silver nanoparticles and E. coli K1 treated with silver alone, compared to untreated E. coli K1. Of note, the expression levels of cation efflux genes (cusA and copA) and translocation of ions, across the membrane genes (rsxB) were found to increase 2.6, 3.1, and 3.3- log FC, respectively. Significant regulation was observed for metabolic genes and several genes involved in the coordination of flagella. Conclusions The antibacterial mechanism of nanoparticles maybe due to disruption of the cell membrane, oxidative stress, and metabolism in E. coli K1. Further studies will lead to a better understanding of the genetic mechanisms underlying treatment with nanoparticles and identification of much needed novel antimicrobial drug candidates.

scholarly journals Evaluation of Changes Induced in the Probiotic Escherichia coli M17 Following Recurrent Exposure to Antimicrobials

2021 ◽  
pp. 158-167
Author(s):  
M. J. A. Mbarga ◽  
I. V. Podoprigora ◽  
E. G. Volina ◽  
A. V. Ermolaev ◽  
L. A. Smolyakova
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Fold Increase ◽  
Bacterial Attachment ◽  
Diffusion Method ◽  
Cross Resistance ◽  
Microdilution Method ◽  
Similar Work

Introduction: It is already well known that the exposure of certain bacteria, pathogenic or not, to antimicrobials is likely to increase their virulence and induce the development of direct or cross resistance to antimicrobials, but there is almost no information available regarding probiotics. Aim: To assess the changes induced in susceptibility to antibiotics, biofilm formation, growth rate and relative pathogenicity in the probiotic Escherichia coli M17 (EC-M17) after long exposure to antimicrobials namely ampicillin, kanamycin, cefazolin and silver nanoparticles (AgNPs). Methods: After determining the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the 4 antimicrobials above-mentioned by the microdilution method, EC-M17 was exposed to increasing subinhibitory doses ranging from MIC/8 to MIC for 8 days. The susceptibility to antibiotics of the mutants obtained was assessed by the Kirby Bauer disc diffusion method, biofilm formation by the Congo red agar method and with crystal violet bacterial attachment assay, and relative pathogenicity was assessed using a Galleria melonella waxworm model. Results: Exposure to antimicrobials induces noticeable changes in EC-M17. The highest adaptation to antimicrobials was observed on AgNPs with 8-fold increase in MIC and 16-fold increase in MBC of AgNPs. EC-M17 exposed to ampicillin, kanamycin and silver nanoparticles became resistant to ampicillin, ceftazidime, ceftazidime/clavulanate and tetracycline while exposure to cefazolin induced a significant decrease in sensitivity to tetracycline and ampicillin and resistance to ceftazidime/clavulanate and ceftazidime. The strain exposed to ampicillin was the only one to produce more biofilm than the control strain and except the EC-M17 exposed to cefazolin, all other EC-M17 strains were more pathogenic on G. melonella model than the control. Conclusion: Data in this investigation suggest that repeated exposure of the probiotic EC-M17 to antimicrobials may induce changes in antimicrobials susceptibility, biofilm formation, growth rate, and relative pathogenicity. Therefore, as far as possible, the probiotic E. coli M17 should not be used in combination with antibiotics and further investigations are required to expand similar work on more probiotics in order to avoid resistance build-up which might be transmitted by horizontal transfer.

Guanidinium and phosphonium scaffolds loaded with silver nanoparticles: synthesis, characterization, in vitro assessment of the antibacterial potential and toxicity

2021 ◽  
Author(s):  
M. N. Gorbunova ◽  
L.M. Lemkina ◽  
A.I. Nechaev
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Uv Spectroscopy ◽  
Planktonic Cells ◽  
Silver Nanocomposites ◽  
Nanoparticles Synthesis ◽  
In Vitro Assessment ◽  
Xrd Techniques ◽  
Antibacterial Potential

Abstract New silver nanocomposites based on polysulfones of 2,2-diallyl-1,1,3,3-tetraethylguanidiniumchloride [poly(AGC-SO2)], tris(diethylamino)diallylaminophosphonium tetrafluoroborate [poly(DAAP-BF4-SO2)] and chloride [poly(DAAP-Cl-SO2)] have been developed. UV- spectroscopy, SEM and XRD techniques were used to characterize the formation of silver nanoparticles in copolymers. Antibacterial action of new silver nanocomposites on S. epidermidis 33 and Escherichia coli (planktonic cells and biofilms) was studied. The silver nanocomposites strongly inhibited biofilms formation of S. epidermidis 33 and Escherichia coli. The silver nanocomposites based on phosphonium polysulfones have a significant cytotoxic activity against RD and MS line cells.

Sign in / Sign up

Export Citation Format

Share Document