Synthesis and In Vitro Bioactivity of Hydroxyapatite/Polyamide66 Nanocomposite

2012 ◽  
Vol 557-559 ◽  
pp. 409-412
Author(s):  
Chen You ◽  
Min Fang Chen ◽  
Rong Zhen Shen ◽  
De Bao Liu
Keyword(s):  
Simulated Body Fluid ◽  
Formic Acid ◽  
Body Fluid ◽  
Room Temperature ◽  
Apatite Layer

A stable n-HA non-aqueous sol was prepared. During the reaction, the gelatin coated the surface of n-HA rod and chemically bonded with the n-HA. PA66 was dissolved in formic acid and then added to n-HA sol with constant stirring for 3 hours at room temperature, a homogenous composite of 40wt.% n-HA and 60wt.% PA66 was successfully synthesized. It was found that no new phases appeared during formulating the n-HA sol and PA66 solution, but the crystallinity of PA66 was lower. The amide groups in the gelatin are same as PA66, the gelatin coated n-HA sol and PA66 solution have very good compatibility. The developed n-HA/PA66 composites were then soaked in a simulated body fluid (SBF) for 1, 2, 3, and 4 weeks, respectively. It was found that the composites immersed for 2 weeks effectively induced a new bone-like apatite layer with lower crystallinity. The n-HA/PA66 composites have much better bioactivity than PA66.

Bioactivity of Silver Doped Hydroxyapatite Scaffolds in Simulated Body Fluids

2014 ◽  
Vol 604 ◽  
pp. 175-179 ◽  
Author(s):  
Lasma Poca ◽  
Arita Dubnika ◽  
Dagnija Loca ◽  
Liga Berzina-Cimdina
Keyword(s):  
Simulated Body Fluid ◽  
Incubation Period ◽  
Powder Diffraction ◽  
Body Fluid ◽  
Body Fluids ◽  
Apatite Layer ◽  
X Ray ◽  
Simulated Body Fluids ◽  
Two Phases

In the present study, thein vitrobioactivity of silver-doped hydroxyapatite (HAp/Ag) scaffolds was investigated. HAp/Ag was prepared using two different modified wet precipitation methods. The X-ray powder diffraction (XRD) results showed, that sintered HAp/Ag samples prepared using method (I) contain two phases HAp and Ag, but samples prepared by method (II) contain three different phases - HAp, Ag and AgO. After 2 month incubation period in simulated body fluid (SBF), surface of HAp/Ag scaffolds was coated with bone-like apatite. Thickness of bone-like apatite layer increased from 2 μm up to 32 μm, increasing the incubation period.

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

scholarly journals Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2137
Author(s):  
Lubomir Medvecky ◽  
Maria Giretova ◽  
Radoslava Stulajterova ◽  
Lenka Luptakova ◽  
Tibor Sopcak
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Calcium Sulfate ◽  
Powder Mixtures ◽  
Liquid Component ◽  
Tetracalcium Phosphate ◽  
Nanocrystalline Hydroxyapatite ◽  
One Step ◽  
Calcium Sulfate Hemihydrate

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

scholarly journals Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 667
Author(s):  
Zexin Wang ◽  
Fei Ye ◽  
Liangyu Chen ◽  
Weigang Lv ◽  
Zhengyi Zhang ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Composite Coating ◽  
Body Fluid ◽  
Composite Coatings ◽  
Degradation Process ◽  
Ceramic Coatings ◽  
Immersion Test ◽  
Substrate Material ◽  
Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

Fabrication of Bioactive Stainless Steel by the Function of Apatite Nuclei

2016 ◽  
Vol 696 ◽  
pp. 151-156 ◽  
Author(s):  
Takeshi Yabutsuka ◽  
Ryoki Karashima ◽  
Shigeomi Takai ◽  
Takeshi Yao
Keyword(s):  
Stainless Steel ◽  
Simulated Body Fluid ◽  
Bonding Strength ◽  
Body Fluid ◽  
Apatite Layer ◽  
Mechanical Interlocking ◽  
High Bonding Strength ◽  
Fluid Formation ◽  
Bonelike Apatite

Micropores were formed on the surfaces of stainless steel (SUS) by sandblasting methods and Apatite Nuclei (AN) were formed in the pores. By this treatments, a bioactive SUS was fabricated. Apatite-forming ability of the SUS was evaluated by immersing in an acellular simulated body fluid. Formation of bonelike apatite was induced on the surface of the SUS within 1 day. High bonding strength of the bonelike apatite layer was achieved by a mechanical interlocking effect between the bonelike apatite formed in the pores and the SUS specimen.

In Vitro and In Vivo Degradation, Mechanical Properties, and Histocompatibility of Weft-Knitted Silk Mesh-Like Grafts

2022 ◽  
Vol 12 (2) ◽  
pp. 411-416
Author(s):  
Liang Tang ◽  
Si-Yu Zhao ◽  
Ya-Dong Yang ◽  
Geng Yang ◽  
Wen-Yuan Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Degradation Rate ◽  
Maximum Load ◽  
Artificial Ligament ◽  
In Vivo Degradation

To investigate the degradation, mechanical properties, and histocompatibility of weft-knitted silk mesh-like grafts, we carried out the In Vitro and In Vivo silk grafts degradation assay. The In Vitro degradation experiment was performed by immersing the silk grafts in simulated body fluid for 1 year, and the results showed that the degradation rate of the silk mesh-like grafts was very slow, and there were few changes in the mechanical properties and quality of the silk mesh-like graft. In Vivo degradation assay was taken by implantation of the silk mesh-like grafts into the subcutaneous muscles of rabbits. At 3, 6, and 12 months postoperation, the rate of mass loss was 19.36%, 31.84%, and 58.77%, respectively, and the maximum load was 63.85%, 34.63%, and 10.76%, respectively of that prior to degradation. The results showed that the degradation rate of the silk graft and the loss of mechanical properties In Vivo were faster than the results obtained in the In Vitro experiments. In addition, there were no significant differences in secretion of serum IL-6 and TNF-α between the experimental and normal rabbits (P >0.05), suggesting no obvious inflammatory reaction. The findings suggest that the weft-knitted silk mesh-like grafts have good mechanical properties, histocompatibility, and In Vivo degradation rate, and therefore represent a candidate material for artificial ligament

Development of Bioactive Titanium-Apatite Nuclei Composite

2007 ◽  
Vol 361-363 ◽  
pp. 709-712 ◽  
Author(s):  
Takeshi Yabutsuka ◽  
Mitsuhiro Hibino ◽  
Takeshi Yao
Keyword(s):  
Simulated Body Fluid ◽  
Adhesive Strength ◽  
Body Fluid ◽  
Apatite Layer ◽  
Mechanical Interlocking ◽  
Composite Surface ◽  
High Adhesive Strength

Apatite nuclei were precipitated in the pores of titanium in simulated body fluid (SBF) and titanium-apatite nuclei composite was obtained. Apatite was induced by the apatite nuclei inside the pores of the composite and apatite layer was formed on the composite surface by soaking in SBF. The apatite layer showed high adhesive strength to the composite due to a mechanical interlocking effect between the composite and the apatite.

scholarly journals In vitro Degradation of β-TCP/PLGA Composites Prepared with Microwave Energy in Simulated Body Fluid

2006 ◽  
Vol 16 (11) ◽  
pp. 676-680 ◽  
Author(s):  
Hyeong-Ho Jin ◽  
Sang-Ho Min ◽  
Yong-Taek Hyun ◽  
Hong-Chae Park ◽  
Seog-Young Yoon
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Microwave Energy ◽  
In Vitro Degradation

Synthesis of sol-gel derived glass powder and in vitro bioactivity property tested in simulated body fluid

2016 ◽  
Author(s):  
S. A. Syed Nuzul Fadzli ◽  
S. Roslinda ◽  
Firuz Zainuddin ◽  
Hamisah Ismail
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Glass Powder ◽  
Sol Gel ◽  
In Vitro Bioactivity
Sign in / Sign up

Export Citation Format

Share Document