Bioactivity of Silver Doped Hydroxyapatite Scaffolds in Simulated Body Fluids

2014 ◽  
Vol 604 ◽  
pp. 175-179 ◽  
Author(s):  
Lasma Poca ◽  
Arita Dubnika ◽  
Dagnija Loca ◽  
Liga Berzina-Cimdina
Keyword(s):  
Simulated Body Fluid ◽  
Incubation Period ◽  
Powder Diffraction ◽  
Body Fluid ◽  
Body Fluids ◽  
Apatite Layer ◽  
X Ray ◽  
Simulated Body Fluids ◽  
Two Phases

In the present study, thein vitrobioactivity of silver-doped hydroxyapatite (HAp/Ag) scaffolds was investigated. HAp/Ag was prepared using two different modified wet precipitation methods. The X-ray powder diffraction (XRD) results showed, that sintered HAp/Ag samples prepared using method (I) contain two phases HAp and Ag, but samples prepared by method (II) contain three different phases - HAp, Ag and AgO. After 2 month incubation period in simulated body fluid (SBF), surface of HAp/Ag scaffolds was coated with bone-like apatite. Thickness of bone-like apatite layer increased from 2 μm up to 32 μm, increasing the incubation period.

In Vitro Study of Microplasma Sprayed Hydroxyapatite Coatings in Simulated Body Fluid

2009 ◽  
Vol 79-82 ◽  
pp. 815-818 ◽  
Author(s):  
Qiu Ying Zhao ◽  
Ding Yong He ◽  
Xiao Yan Li ◽  
Jian Min Jiang
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Carbonate Apatite ◽  
In Vitro Test ◽  
X Ray Diffraction ◽  
X Ray ◽  
Amorphous Phases ◽  
Hydroxyapatite Coatings ◽  
Vitro Test

Hydroxyapatite (HA) coatings were deposited onto Ti6Al4V substrate by microplasma spraying (MPS) in the current research. The morphology, phase compositions, and percentage of crystallinity of the coatings were characterized by means of scanning electron microscopy (SEM) and X-ray diffraction. An in vitro evaluation by soaking the coatings in simulated body fluid (SBF) for up to 14 days was conducted aiming at the evaluation of their bioactivity. Results from the present investigation suggest that microplasma sprayed HA coatings exhibited certain roughness, pores, and microcracks. Thermal decomposition existed in the coatings where HA, α-TCP，β-TCP, amorphous phases, and CaO-exclusive impurities were observed. The in vitro test indicated that HA coatings deposited by MPS possessed better bioactivity and stability. A layer of carbonate-apatite covered most of the coating surface, which did not exhibit significant spalling after incubation in SBF.

scholarly journals The Atomic-Scale Interaction of Bioactive Glasses with Simulated Body Fluid

2005 ◽  
Vol 480-481 ◽  
pp. 21-26 ◽  
Author(s):  
L.J. Skipper ◽  
F.E. Sowrey ◽  
D.M. Pickup ◽  
R.J. Newport ◽  
K.O. Drake ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Sol Gel ◽  
Atomic Scale ◽  
X Ray Diffraction ◽  
Bioactive Glasses ◽  
Edge Structure ◽  
X Ray ◽  
X Ray Absorption

The formation of a carbonate-containing hydroxyapatite, HCAp, layer on bioactive calcium silicate sol-gel glass of the formula (CaO)0.3(SiO2)0.7 has been studied in-vitro in Simulated Body Fluid (SBF). Extended X-ray Absorption Fine Structure (EXAFS), X-ray Absorption Near Edge Structure (XANES), X-ray diffraction (XRD), and solid state nuclear magnetic resonance (NMR) measurements have been performed with results showing the formation of a significantly amorphous HCAp layer after less than 5 hours in solution.

Studies on Development, Bioactivity and Corrosion Behaviour of Nanostructured Titania/Hydroxyapatite Composite Layer on Cp Ti

2011 ◽  
Vol 471-472 ◽  
pp. 325-330 ◽  
Author(s):  
K. Venkateswarlu ◽  
N. Rameshbabu ◽  
Arumugam Chandra Bose ◽  
V. Muthupandi ◽  
S. Subramanian
Keyword(s):  
Corrosion Resistance ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Composite Layer ◽  
In Vitro Bioactivity ◽  
X Ray ◽  
Composite Layers ◽  
Cp Ti ◽  
Nanostructured Titania

Nanostructured titania/hydroxyapatite (HA) composite layer was developed on commercially pure titanium (Cp Ti) implant material by plasma electrolytic processing (PEP) technique in order to improve its bioactivity and corrosion resistance under physiological conditions. The phases present in the developed composite layer were studied by X-ray diffraction (XRD) technique. The surface morphology and thickness of the composite layers were observed by scanning electron microscopy (SEM). The corrosion characteristics of the developed layer were studied by potentiodynamic polarization scan under simulated body fluid (7.4 pH Hanks solution) and simulated osteoclast (4.5 pH) conditions. The in-vitro bioactivity of the composite layers was studied by using Kokubu’s simulated body fluid (SBF) solution. The X-ray diffractograms reveal the presence of anatase TiO2 and HA phases in the developed layer. The SEM results confirm the pore-free morphology of the implant material surface and the thickness of the developed composite layer was observed to be 110 ± 5 µm for 12 min of PEP. The potentiodynamic polarization study shows an improved corrosion resistance and the in-vitro bioactivity test results indicate enhanced apatite forming ability of PEP treated Cp Ti surfaces compared to that of the untreated Cp Ti, under simulated body fluid conditions.

Synthesis and In Vitro Bioactivity of Hydroxyapatite/Polyamide66 Nanocomposite

2012 ◽  
Vol 557-559 ◽  
pp. 409-412
Author(s):  
Chen You ◽  
Min Fang Chen ◽  
Rong Zhen Shen ◽  
De Bao Liu
Keyword(s):  
Simulated Body Fluid ◽  
Formic Acid ◽  
Body Fluid ◽  
Room Temperature ◽  
Apatite Layer

A stable n-HA non-aqueous sol was prepared. During the reaction, the gelatin coated the surface of n-HA rod and chemically bonded with the n-HA. PA66 was dissolved in formic acid and then added to n-HA sol with constant stirring for 3 hours at room temperature, a homogenous composite of 40wt.% n-HA and 60wt.% PA66 was successfully synthesized. It was found that no new phases appeared during formulating the n-HA sol and PA66 solution, but the crystallinity of PA66 was lower. The amide groups in the gelatin are same as PA66, the gelatin coated n-HA sol and PA66 solution have very good compatibility. The developed n-HA/PA66 composites were then soaked in a simulated body fluid (SBF) for 1, 2, 3, and 4 weeks, respectively. It was found that the composites immersed for 2 weeks effectively induced a new bone-like apatite layer with lower crystallinity. The n-HA/PA66 composites have much better bioactivity than PA66.

scholarly journals Improved bioactivity of GUMMETAL®, Ti59Nb36Ta2Zr3O0.3, via formation of nanostructured surfaces

2018 ◽  
Vol 9 ◽  
pp. 204173141877417 ◽  
Author(s):  
Shiva Kamini Divakarla ◽  
Seiji Yamaguchi ◽  
Tadashi Kokubo ◽  
Dong-Wook Han ◽  
Jae Ho Lee ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Cell Attachment ◽  
Cell Number ◽  
Calcium Titanate ◽  
Apatite Formation ◽  
X Ray ◽  
Nanostructured Surfaces ◽  
Implant Integration

The leading reason for implant revision surgery globally is lack of implant integration with surrounding bone. A new titanium alloy GUMMETAL® (Ti59Nb36Ta2Zr3O0.3) is currently used in biomedical devices and has a Young’s modulus that is better matched to bone. The surface was subject to NaOH, CaCl2, heat and water treatment (BioGum) after which the surfaces were evaluated using atomic force microscope, scanning electron microscope, X-ray diffractometer and elemental analysis using energy dispersive X-ray. To demonstrate enhanced bone bonding ability and cytocompatibility, apatite formation in simulated body fluid and in vitro stem cell attachment, proliferation and cytoskeleton organisation were examined. The formation of a ~200 nm nanoscale needle-like calcium titanate network on the surface following treatment was revealed and upon soaking in simulated body fluid, the formation of a ~5 µm layer of apatite. Metabolic activity of rat bone marrow stem cells on BioGum was increased in comparison to control and the cell number appeared greater, with more elongated morphology as early as 2 h post-seeding. This positions the modification as a simple and potentially universal technology for the improvement of implant integration.

Bioactivity of Plasma-Sprayed TiO2 Coating in Simulated Body Fluid by Hydrofluoric Acid Treatment

2006 ◽  
Vol 510-511 ◽  
pp. 13-16
Author(s):  
Xiao Bing Zhao ◽  
Xuan Yong Liu ◽  
Chuan Xian Ding
Keyword(s):  
Titanium Alloy ◽  
Simulated Body Fluid ◽  
Hydrofluoric Acid ◽  
Body Fluid ◽  
Acid Treatment ◽  
Atmospheric Plasma ◽  
X Ray ◽  
Tio2 Coatings ◽  
Plasma Sprayed

TiO2 coatings on titanium alloy substrates were prepared by atmospheric plasma spraying using commercial nano-powders. Then, as-sprayed coatings were treated using 10% hydrofluoric acid (HF) at room temperature for 30 seconds. As-sprayed and HF-treated titania coatings were soaked in simulated body fluid to investigate the formation of apatite on their surface. Field-emission scanning electron microscopy was used to observe the surface morphologies, and the phase composition of the as-sprayed coating and apatite were analyzed by X-ray diffraction and energy-dispersive X-ray spectrometry. As-sprayed titania coating is composed of rutile, anatase, and a small quantity of Ti3O5. It exhibited excellent adhesion between the TiO2 coatings and titanium alloy substrates, and the bonding strength was about 38 MPa. After in vitro experiment, a new substance containing calcium and phosphate was formed on the surface of HF-treated TiO2 coatings after being soaked in SBF, while the new substance was not formed on the surface of as-sprayed TiO2 coatings. The results indicated that the bioactivity can be induced to the surface of plasma sprayed TiO2 coatings by hydrofluoric acid treatment.

Micro-Topography and Reactivity of Implant Surfaces: An In Vitro Study in Simulated Body Fluid (SBF)

2015 ◽  
Vol 21 (1) ◽  
pp. 190-203 ◽  
Author(s):  
M.G. Gandolfi ◽  
P. Taddei ◽  
F. Siboni ◽  
V. Perrotti ◽  
G. Iezzi ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Calcium Phosphates ◽  
Raman Band ◽  
Biologically Active ◽  
Textured Surface ◽  
X Ray ◽  
Micro Topography ◽  
Element Mapping

AbstractThe creation of micro-textured dental implant surfaces possessing a stimulating activity represents a challenge in implant dentistry; particularly, the formation of a thin, biologically active, calcium-phosphate layer on their surface could help to strengthen the bond to the surrounding bone. The aim of the present study was to characterize in terms of macrostructure, micro-topography and reactivity in simulated body fluid (SBF), the surface of titanium (Ti) implants blasted with TiO2 particles, acid etched with hydrofluoric acid, and activated with Ca and Mg-containing nanoparticles. Sandblasted and acid-etched implants were analyzed by ESEM-EDX (environmental scanning electron microscope with energy dispersive X-ray system) to study the micromorphology of the surface and to perform elemental X-ray microanalysis (microchemical analyses) and element mapping. ESEM-EDX analyses were performed at time 0 and after a 28-day soaking period in SBF Hank’s balanced salt solution (HBSS) following ISO 23317 (implants for surgery—in vitro evaluation for apatite-forming ability of implant materials). Microchemical analyses (weight % and atomic %) and element mapping were carried out to evaluate the relative element content, element distribution, and calcium/phosphorus (Ca/P) atomic ratio. Raman spectroscopy was used to assess the possible presence of impurities due to manufacturing and to investigate the phases formed upon HBSS soaking. Micro-morphological analyses showed a micro-textured, highly rough surface with microgrooves. Microchemical analyses showed compositional differences among the apical, middle, and distal thirds. The micro-Raman analyses of the as-received implant showed the presence of amorphous Ti oxide and traces of anatase, calcite, and a carbonaceous material derived from the decomposition of an organic component of lipidic nature (presumably used as lubricant). A uniform layer of Ca-poor calcium phosphates (CaPs) (Ca/P ratio <1.47) was observed after soaking in HBSS; the detection of the 961 cm−1 Raman band confirms this finding. These implants showed a micro-textured surface supporting the formation of CaPs when immersed in SBF. These properties may likely favor bone anchorage and healing by stimulation of mineralizing cells.

In Vitro Bioactivity of Composite of Nanophase Titania/Bioactive Glass-Ceramic in Simulated Body Fluid

2005 ◽  
Vol 288-289 ◽  
pp. 171-174
Author(s):  
Hui Wang ◽  
Bang Cheng Yang ◽  
Qi Feng Yu ◽  
Dayi Wu ◽  
Xing Dong Zhang
Keyword(s):  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Glass Ceramic ◽  
Body Fluid ◽  
Mechanical Analysis ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biomechanical Compatibility

Titania ceramics is lack of bone-bonding ability even if it has excellent biocompatibility. Recently, it is even found that the nanophase titania ceramics could enhance the proliferation of osteoblasts. If the bone-bonding ability of this material is improved, it would be a potential bone replacement material. Bioactive glass-ceramic (BGC) is provided with the best bioactivity in biomaterials. In this study, the apatite formation ability and the mechanic properties of titania ceramic were investigated by the accession of BGC. Four samples: TiO2 ceramic, TiO2 +10%BGC, TiO2 +20%BGC and BGC were prepared respectively. These ceramics were exposed to a simulated body fluid (SBF) for 7, 14 and 21d. Scanning electron microscopy (SEM), energy dispersive X-ray detector (EDX) and thin film X-ray diffraction (TF-XRD) results showed that the apatite formation of the ceramics was improved by adding BGC into nanophase titania ceramic. The mechanical analysis showed the biomechanical compatibility was also improved by adding BGC into nanophase titania ceramic.

Influence of Strontium on In Vitro Bioactivity of Heat-Treated Porous Ca-P Ceramics on Titanium for Biomedical Applications

2011 ◽  
Vol 493-494 ◽  
pp. 453-457
Author(s):  
Kuan Chen Kung ◽  
Tzer Min Lee ◽  
Truan Sheng Lui
Keyword(s):  
Body Fluid ◽  
Biomedical Applications ◽  
Clinical Applications ◽  
Apatite Layer ◽  
X Ray Diffraction ◽  
Apatite Phase ◽  
X Ray ◽  
Heat Treated ◽  
Scanning Electron

The bioactivity of materials was evaluated based on the ability to induce a bond-like apatite layer on the surface in simulated body fluid (SBF). The aim of this study was to investigate the coatings containing strontium on bioactivity after heat treatment. After the materials were soaked in SBF for 1 day, precipitates did not form on the surface of heat-treated MAO coating without strontium. The precipitates were observed on surface of heat-treated MAO coatings containing strontium. After 7 days, the surface of heat-treated MAO coatings containing strontium was completely covered with precipitates. The precipitates were found to be composed of fiber structures using scanning electron microscope (SEM). The phase was identified as the apatite phase using thin film X-ray diffraction (TF-XRD). The results show that heat-treated MAO coatings containing strontium can induce the formation of an apatite layer on their surface. All finding in this study indicated that heat-treated MAO coatings containing strontium have good bioactivity for clinical applications.

In Vitro Behavior of Magnesium Apatite Coatings in Organic Modified Simulated Body Fluid

2011 ◽  
Vol 299-300 ◽  
pp. 508-511
Author(s):  
Guo Chao Qi ◽  
Feng Jun Shan ◽  
Qiang Li ◽  
Jing Yuan Yu ◽  
Qu Kai Zhang
Keyword(s):  
Simulated Body Fluid ◽  
Photoelectron Spectroscopy ◽  
Body Fluid ◽  
Sol Gel ◽  
Dip Coating ◽  
X Ray ◽  
Coating Method ◽  
Sbf Solution ◽  
Dip Coating Method

Magnesium apatite (MA, (Ca9Mg)(PO4)6(OH)2) and Hydroxyapatite (HA) coatings were synthesized on Ti6Al4V substrates by a sol-gel dip coating method. Glucose and bovine serum albumin (BSA) were added to the standard simulated body fluid (SBF) separately to form organic-containing simulated body fluids. MA and HA coatings were immersed in standard and organic modified SBF for time periods of 4, 7, 14, 21 and 28 days at 37±1°C. The surface dissolution and deposition behavior of the coatings after soaking were examined with Scanning Electron Microscopy (SEM) and X-ray Photoelectron Spectroscopy (XPS). The results show that glucose in SBF has no apparent effect on the deposition of new apatite from the solution. BSA in SBF shows retardation effect on the deposition of apatite by forming a protein dominant globular layer. This layer inhibits the further deposition of apatite from SBF solution.

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