Antioxidant Properties of Deer Blood Hydrolysate and the Possible Mode of Action

Deer blood was hydrolyzed using Alcalase with hydrolysis time ranged form 0 to 6 h, and the degree of hydrolysis (DH) of protein hydrolysates increased with increasing hydrolysis time (P < 0.05). The reducing power, radicals scavenging activities and Cu2+-chelation ability of deer blood hydrolysate (DBH) significantly enhanced with increasing hydrolysis time (P < 0.05). The antioxidant activity of DBH, indicated by thiobarbituric acid-reactive substance (TBARS) values in a liposome-oxidizing system, increased with increasing DH (P < 0.05). The results indicated that antioxidant activity of DBH depended on hydrolysis time, and the hydrolyzed deer blood could be a potent food antioxidant.

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Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane

Antioxidants ◽

10.3390/antiox8100495 ◽

2019 ◽

Vol 8 (10) ◽

pp. 495

Author(s):

Qian-Cheng Zhao ◽

Jie-Yuan Zhao ◽

Dong Uk Ahn ◽

Yong-Guo Jin ◽

Xi Huang

Keyword(s):

Antioxidant Activity ◽

Water Solubility ◽

Biological Activities ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Exchange Chromatography ◽

Eggshell Membrane ◽

Degree Of Hydrolysis ◽

Hydrolysis Time ◽

Antioxidant Agents

The enzymatic hydrolysates (EHs) of the eggshell membrane (ESM) were obtained after incubating eggshell membrane in solutions prepared with Na2SO3 and alkaline protease combinations. The effects of enzyme species, enzyme dosage, Na2SO3 concentration, and hydrolysis time on the antioxidant activity of the ESM-EH were determined. Also, the correlation between the degree of hydrolysis (DH) and the antioxidant activity of ESM-EH was analyzed. The DH of ESM-EH showed a highly positive correlation with the reducing power (R2 = 0.857) and total antioxidant activity (TAA) (R2 = 0.876) and performed negative correlation with the Fe2+-chelating ability (R2 = −0.529). The molecular weight distribution of the ESM-EH was determined by MALDI-TOF/MS. Cation exchange chromatography (Sephadex C-25) was used to isolate the ESM-EH and then the enzymatic hydrolysis fragment (EHF) was obtained. Among the five isolated fragments (F1~F5), fragment 3 (F3), which was composed of 28 polypeptides, showed the highest ability to quench ABTS• (2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid) (90.44%) and also displayed stronger TBARS (thiobarbituric acid– reactive substances) (58.17%) and TAA (303.82 µg /mL) than the ESM-EH. Further analysis of the 28 peptides in F3 identified using LC-MS/MS indicated that five peptides (ESYHLPR, NVIDPPIYAR, MFAEWQPR, LLFAMTKPK, MLKMLPFK) showed high water-solubility, biological activities, and antioxidant characteristics. Finally, the TAA of the synthetic peptide was verified, the synthetic peptides ESYHLPR and MFAEWQPR performed the best activity and have high potentials to be used as antioxidant agents in functional foods, pharmaceuticals, or cosmetics.

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Study of irradiated black pepper antioxidant activity changes

Czech Journal of Food Sciences ◽

10.17221/10656-cjfs ◽

2004 ◽

Vol 22 (SI - Chem. Reactions in Foods V) ◽

pp. S187-S190

Author(s):

M. Suhaj ◽

J. Rácová

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Electron Paramagnetic Resonance Spectroscopy ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Black Pepper ◽

Piper Nigrum ◽

Resonance Spectroscopy

Some antioxidant activities of extracts of irradiated black pepper (Piper nigrum) were evaluated. The ground black pepper was exposed to gamma-irradiation at doses from 5 to 30 kGy. The effect of irradiation on antioxidant properties of black pepper extracts was investigated by these methods: radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, determination of reducing power, thiobarbituric acid reactive substances assay and electron paramagnetic resonance spectroscopy (EPR). Irradiation resulted in a decrease of DPPH antiradical activity of black pepper extracts according to dose of irradiation. Reducing power was not changed by action of irradiation. Very important and sensitive differences in antioxidant activity of irradiated black pepper were investigated by EPR spectrometry and thiobarbituric acid method.

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IN VIVO ANTIOXIDANT ACTIVITY OF LIMNOPHILA HETEROPHYLLA AND MICHELIA CHAMPACA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i12.22013 ◽

2017 ◽

Vol 9 (12) ◽

pp. 241 ◽

Cited By ~ 1

Author(s):

Raja S, ◽

Ravindranadh K.

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Thiobarbituric Acid ◽

Thiobarbituric Acid Reactive Substance ◽

Reactive Substance ◽

Reducing Ability ◽

Liver And Kidney ◽

Michelia Champaca ◽

Different Doses

Objective: The present study was aimed at investigating the in-vivo antioxidant activity of the methanol extracts of Limnophila heterophylla and Michelia champaca leaves.Methods: Methanol extract of both plants were administered to rats separately at three different doses of 125, 250 and 500 mg/kg for 21 d to evaluate oxidative stress parameters such as ferric reducing ability of plasma (FRAP), thiobarbituric acid reactive substance (TBARS) and reduced glutathione (GSH) and to evaluate antioxidant enzyme levels of catalase (CAT) and superoxide dismutase (SOD).Results: The methanol extracts of both the plants significantly (p<0.05) elevated the ferric reducing ability of plasma (FRAP) on days 7, 14 and 21 of treatment. Significant (p<0.05) decrease of thiobarbituric acid reactive substance (TBARS) levels along with an increase in the superoxide dismutase (SOD) enzyme level in the liver and kidney at three different doses both the plants was observed. Treatment at a dose of 500 mg/kg b. w of both plants caused a significant increase only in the level of CAT in the liver and kidney. However, there was no significant effect of a thiobarbituric acid reactive substance (TBARS), superoxide dismutase (SOD) and catalase (CAT) in the heart and reduced glutathione (GSH) level in liver, heart and kidney at three different doses both the plants.Conclusion: These outcomes recommend that the leaves of Limnophila heterophylla and Michelia champaca have a potent antioxidant activity which may be responsible for some of its reported pharmacological actions.

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Identification of Microbial Flora in Dry Aged Beef to Evaluate the Rancidity during Dry Aging

Processes ◽

10.3390/pr9112049 ◽

2021 ◽

Vol 9 (11) ◽

pp. 2049

Author(s):

Sejeong Kim ◽

Jong-Chan Kim ◽

Sunhyun Park ◽

Jinkwi Kim ◽

Yohan Yoon ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Thiobarbituric Acid ◽

Microbial Flora ◽

Thiobarbituric Acid Reactive Substance ◽

Rt Pcr ◽

Chain Reaction ◽

Reactive Substance ◽

Basic Nitrogen ◽

Polymerase Chain ◽

Tbars Values

Dry aging creates a unique taste and flavor in beef; however, the process also causes rancidity, which is harmful to humans. During dry aging, the microbial flora in beef changes continuously; thus, this change can be used as an indicator of rancidity. The objective of this study was to analyze the correlation between microbial flora in beef and rancidity during dry aging. The round of beef (2.5–3 kg) was dry aged under 1.5 ± 1 °C and 82 ± 5% moisture for 17 weeks. The microflora in the dry aged beef was analyzed by pyrosequencing. The volatile basic nitrogen (VBN) and thiobarbituric acid reactive substance (TBARS) values were also measured. Primers were designed to detect and quantify bacteria using real-time polymerase chain reaction (RT-PCR). The VBN and TBARS values in the dry aged beef depreciated from week 11 of aging. The levels of Streptococcus spp., Pantoea spp., and Pseudomonas spp. significantly changed at around week 11. Quantitative RT-PCR showed that the levels of Pantoea spp. and Streptococcus spp. could be used to identify rancidity during dry aging. Thus, among the microbial flora in dry aged beef, Pantoea spp. and Streptococcus spp. can be used to determine the rancidity of dry aged beef.

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Cardioprotective effects of carvedilol on acute autoimmune myocarditis: anti-inflammatory effects associated with antioxidant property

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00536.2003 ◽

2004 ◽

Vol 286 (1) ◽

pp. H83-H90 ◽

Cited By ~ 63

Author(s):

Zuyi Yuan ◽

Keisuke Shioji ◽

Yasuki Kihara ◽

Hiroyuki Takenaka ◽

Yoko Onozawa ◽

...

Keyword(s):

Inflammatory Cytokines ◽

Antioxidant Properties ◽

Thiobarbituric Acid ◽

Experimental Models ◽

Left Ventricular ◽

Thiobarbituric Acid Reactive Substance ◽

Autoimmune Myocarditis ◽

Reactive Substance

Carvedilol, a new β-blocker with antioxidant properties, has been shown to be cardioprotective in experimental models of myocardial damage. We investigated whether carvedilol protects against experimental autoimmune myocarditis (EAM) because of its suppression of inflammatory cytokines and its antioxidant properties. We orally administered a vehicle, various doses of carvedilol, racemic carvedilol [ R(+)-carvedilol, an enantiomer of carvedilol without β-blocking activity], metoprolol, or propranolol to rats with EAM induced by porcine myosin for 3 wk. Echocardiographic study showed that the three β-blockers, except R(+)-carvedilol, suppressed left ventricular fractional shortening and decreased heart rates to the same extent. Carvedilol and R(+)-carvedilol, but not metoprolol or propranolol, markedly reduced the severity of myocarditis at the two different doses and suppressed thickening of the left ventricular posterior wall in rats with EAM. Only carvedilol suppressed myocardial mRNA expression of inflammatory cytokines and IL-1β protein expression in myocarditis. In addition, carvedilol and R(+)-carvedilol decreased myocardial protein carbonyl contents and myocardial thiobarbituric acid-reactive substance products in rats with EAM. The in vitro study showed that carvedilol and R(+)-carvedilol suppressed IL-1β production in LPS-stimulated U937 cells and that carvedilol and R(+)-carvedilol, but not metoprolol or propranolol, suppressed thiobarbituric acid-reactive substance products in myocardial membrane challenged by oxidative stress. It was also confirmed that probucol, an antioxidant, ameliorated EAM in vivo. Carvedilol protects against acute EAM in rats, and the superior cardioprotective effect of carvedilol compared with metoprolol and propranolol may be due to suppression of inflammatory cytokines associated with the antioxidant properties in addition to the hemodynamic modifications.

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Chemical Analysis and Antioxidant Activity of the Essential Oils of Three Piperaceae Species Growing in the Central Region of Cuba

Natural Product Communications ◽

10.1177/1934578x1300800935 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 3

Author(s):

Elisa Jorge Rodríguez ◽

Yanelis Saucedo-Hernández ◽

Yvan Vander Heyden ◽

Ernesto F. Simó-Alfonso ◽

Guillermo Ramis-Ramos ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oils ◽

Central Region ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Gas Chromatography Mass Spectrometry ◽

Radical Scavenging Ability ◽

Main Components

The present study describes the phytochemical profile and antioxidant activity of the essential oils of three Piperaceae species collected in the central region of Cuba. The essential oils of Piper aduncum, P. auritum and P. umbellatum leaves, obtained by hydrodistillation, were analyzed by gas chromatography-mass spectrometry. The main components of P. aduncum oil were piperitone (34%), camphor (17.1%), camphene (10.9%), 1,8-cineol (8.7%) and viridiflorol (7.4%), whereas that of P. auritum and P. umbellatum was safrole (71.8 and 26.4%, respectively). The antioxidant properties of the essential oils were also evaluated using several assays for radical scavenging ability (DPPH test and reducing power) and inhibition of lipid oxidation (ferric thiocyanate method and evaluation against Cucurbita seed oil by peroxide, thiobarbituric acid and p-anisidine methods). P. auritum showed the strongest antioxidant activity among the Piper species investigated, but lower than those of butylated hydroxyanisol and propyl gallate.

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Onion waste as a rich source of antioxidants for meat products

Czech Journal of Food Sciences ◽

10.17221/68/2018-cjfs ◽

2019 ◽

Vol 37 (No. 4) ◽

pp. 268-275

Author(s):

Jan Bedrníček ◽

Ivana Laknerová ◽

Zuzana Linhartová ◽

Jaromír Kadlec ◽

Eva Samková ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Meat Products ◽

Thiobarbituric Acid ◽

Natural Antioxidants ◽

The European Union ◽

Water Extracts ◽

Onion Skin ◽

Pork Patties ◽

Tbars Values

Onion skin is a waste produced in thousands of tons annually in the European Union. It is a rich natural source of flavonoids and its water extracts (as an environmentally friendly solvent) could be used as an antioxidant material for meat products. Therefore, antioxidant properties of onion skin water extracts (OSWEs) were tested on cooked pork patties. Pork patties were divided into five treatments: control (no antioxidant), 10 and 20% (w/w) of yellow OSWE, and 10 and 20% (w/w) of red OSWE. Antioxidant activity, total polyphenols, thiobarbituric acid reactive substances (TBARS), and sensory analysis were assessed. Patties with added antioxidants showed significantly (P < 0.05) higher antioxidant activity and total polyphenol content. Samples with OSWEs, after 5-day storage (5°C), had significantly (P < 0.05) lower TBARS values compared to control. Two main phenolic compounds were identified in OSWEs by liquid chromatography – mass spectrometry using electrospray ionisation in negative mode: quercetin (m/z 301) and quercetin monoglucoside (m/z 463). OSWEs demonstrated the potential to be used as a source of natural antioxidants with strong antioxidant activity in meat products.

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Aktivitas Antioksidan Ekstrak Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) Pada Asam Linoleat

Jurnal MIPA ◽

10.35799/jm.6.2.2017.17816 ◽

2017 ◽

Vol 6 (2) ◽

pp. 86

Author(s):

Jeremy Fransisco Pakasi ◽

Lidya I Momuat ◽

Harry S.J. Koleangan

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Ethanol Extract ◽

Thiobarbituric Acid ◽

Total Phenolic ◽

Thiobarbituric Acid Reactive Substance ◽

Peroxide Formation ◽

Reactive Substance ◽

Percent Inhibition ◽

Ferric Thiocyanate

Penelitian ini bertujuan untuk mempelajari aktivitas antioksidan eksrak tumbuhan suruhan Peperomia pellucida (L.) Kunth pada asam linoleat. Tumbuhan suruhan diekstrak dengan pelarut etanol 80% dan n-heksana dengan cara maserasi selama 48 jam. Ekstrak etanol dan n-heksana dari tumbuhan suruhan diukur kandungan total fenoliknya dengan metode Folin-ciocalteu, serta diuji aktivitas antioksidannya pada asam linoleat menggunakan metode Ferric Thiocyanate untuk menghitung persen penghambatan peroksida, dan metode Thiobarbituric Acid Reactive Substance untuk mengukur persen penghambatan pembentukan malonaldehida. Hasil penelitian kandungan total fenolik dalam ekstrak etanol dan n-heksana tumbuhan suruhan berturut-turut adalah 53.469 mg/kg dan 22.755 mg/kg. Aktivitas antioksidan dari ekstrak etanol tumbuhan suruhan dengan konsentrasi 100 dan 200 µg/mL dalam menghambat pembentukan peroksida berturut-turut sebesar 83.74% dan 88.80%, serta pembentukan malonaldehida sebesar 93.07% dan 93.96% pada asam linoleat.Sedangkan Aktivitas antioksidan dari ekstrak n-heksana tumbuhan suruhan dengan konsentrasi 100 dan 200 µg/mL dalam menghambat pembentukan peroksida berturut-turut sebesar 67.96% dan 73.18%, serta pembentukan malonaldehida sebesar 90.98% dan 92.00% pada asam linoleat.Penelitian ini menyimpulkan bahwa kandungan total fenolik ekstrak etanol tumbuhan suruhan lebih tinggi daripada ekstrak n-heksana, serta aktivitas antioksidan ekstrak etanol adalah yang terbaik dalam menghambat pembentukan peroksida dan malonaldehida pada asam linoleat.This reaserchwas aimed to study the antioxidant activity of Peperomia pellucida (L.) Kunth on linoleic acid. The plant was extracted with 80% ethanol and n-hexane solvent by maceration for 48 hours. The content of total phenolic was measured using the Folin-Ciocalteu method and the antioxidant activity of Peperomia p. wastested on linoleic acid using Ferric Thiocyanate method to calculate the percent inhibition of peroxide and using Thiobarbituric Acid Reactive Substance method for measuring the percent inhibition of malonaldehyde. Total phenolic content of the ethanol extract and the n-hexane extract of Peperomia p. were 53,469 mg/kg and22.755 mg/kg respectively. The antioxidant activities of ethanol extract of Peperomia p. with concentration of 100 and 200 μg/mL in inhibition of peroxide formation were 83,74% and 88,80%, and for malonaldehyde were 93,07% and 93,96% respectively. Whereasthose of n-hexana extracts with the same concentration inhibited 67.96% and 73.18% of peroxide formation, and 90.98% and 92.00% of malonaldehyde formation. Thus,Total content of phenolics of ethanol extract is higher than that of n-hexane extract, similarly the antioxidant activity of ethanol extract is the better in inhibiting the formation of peroxide and malonaldehyde in linoleic acid than that of n-hexana extract.

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Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofAframomum meleguetaMethanolic Seed Extract

Journal of Tropical Medicine ◽

10.1155/2014/159343 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 11

Author(s):

Samuel Okwudili Onoja ◽

Yusuf Ndukaku Omeh ◽

Maxwell Ikechukwu Ezeja ◽

Martins Ndubuisi Chukwu

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Thiobarbituric Acid ◽

Seed Extract ◽

Assay Method ◽

Control Group ◽

Thiobarbituric Acid Reactive Substance ◽

Reactive Substance ◽

Photometric Assay ◽

Antioxidant Effects

Aframomum meleguetaSchum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract ofA. melegueta. The antioxidant effects were evaluated usingin vitro, 2, 2-diphenylpicrylhydrazine photometric assay andin vivoserum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed ofA. meleguetahas potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

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Optimization of Enzymatic Hydrolysis by Alcalase and Flavourzyme To Enhance the Antioxidant Properties of Jasmine Rice Bran Protein Hydrolysate

10.21203/rs.3.rs-1121116/v1 ◽

2021 ◽

Author(s):

Kanrawee Hunsakul ◽

Thunnop Laokuldilok ◽

Vinyoo Sakdatorn ◽

Wannaporn Klangpetch ◽

Niramon Utama-ang

Keyword(s):

Molecular Weight ◽

Antioxidant Activity ◽

Rice Bran ◽

Protein Hydrolysate ◽

Antioxidant Properties ◽

Degree Of Hydrolysis ◽

Hydrolysis Time ◽

Rice Bran Protein ◽

High Antioxidant Activity ◽

Jasmine Rice

Abstract This study aimed to optimize the hydrolysis conditions for producing jasmine rice bran protein hydrolysate (JBH) using response surface methodology (RSM). The independent variables were the ratio of flavourzyme to alcalase (Fl: Al; 0: 100 to 15: 85; 2.84% enzyme concentration) and hydrolysis time (60–540 min). The optimum hydrolysate was obtained at an Fl: Al ratio of 9.81: 90.19 for 60 min, since it enabled high amounts of protein, high antioxidant activity and more low molecular weight proteins. The experimental values obtained were a degree of hydrolysis (DH) of 7.18%, a protein content of 41.73%, an IC50 for DPPH of 6.59 mg/mL, an IC50 for ABTS of 0.99 mg/mL, FRAP of 724.81 mmol FeSO4/100 g, and 322.35 and 479.05 mAU*s for peptides with a molecular weight of <3 and 3–5 kDa, respectively. Using a mixture of enzymes revealed the potential of mixed enzymes to produce JBH containing more small peptides and high antioxidant activity.

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