Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane

The enzymatic hydrolysates (EHs) of the eggshell membrane (ESM) were obtained after incubating eggshell membrane in solutions prepared with Na2SO3 and alkaline protease combinations. The effects of enzyme species, enzyme dosage, Na2SO3 concentration, and hydrolysis time on the antioxidant activity of the ESM-EH were determined. Also, the correlation between the degree of hydrolysis (DH) and the antioxidant activity of ESM-EH was analyzed. The DH of ESM-EH showed a highly positive correlation with the reducing power (R2 = 0.857) and total antioxidant activity (TAA) (R2 = 0.876) and performed negative correlation with the Fe2+-chelating ability (R2 = −0.529). The molecular weight distribution of the ESM-EH was determined by MALDI-TOF/MS. Cation exchange chromatography (Sephadex C-25) was used to isolate the ESM-EH and then the enzymatic hydrolysis fragment (EHF) was obtained. Among the five isolated fragments (F1~F5), fragment 3 (F3), which was composed of 28 polypeptides, showed the highest ability to quench ABTS• (2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid) (90.44%) and also displayed stronger TBARS (thiobarbituric acid– reactive substances) (58.17%) and TAA (303.82 µg /mL) than the ESM-EH. Further analysis of the 28 peptides in F3 identified using LC-MS/MS indicated that five peptides (ESYHLPR, NVIDPPIYAR, MFAEWQPR, LLFAMTKPK, MLKMLPFK) showed high water-solubility, biological activities, and antioxidant characteristics. Finally, the TAA of the synthetic peptide was verified, the synthetic peptides ESYHLPR and MFAEWQPR performed the best activity and have high potentials to be used as antioxidant agents in functional foods, pharmaceuticals, or cosmetics.

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Antioxidant Properties of Deer Blood Hydrolysate and the Possible Mode of Action

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.634-638.1435 ◽

2013 ◽

Vol 634-638 ◽

pp. 1435-1440 ◽

Cited By ~ 2

Author(s):

Shuai Wang ◽

Li Cheng Zhong ◽

Xue Chao Zhai ◽

Dong Dong Yin ◽

Xin Yu Wu

Keyword(s):

Antioxidant Activity ◽

Mode Of Action ◽

Antioxidant Properties ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Degree Of Hydrolysis ◽

Thiobarbituric Acid Reactive Substance ◽

Hydrolysis Time ◽

Reactive Substance ◽

Tbars Values

Deer blood was hydrolyzed using Alcalase with hydrolysis time ranged form 0 to 6 h, and the degree of hydrolysis (DH) of protein hydrolysates increased with increasing hydrolysis time (P < 0.05). The reducing power, radicals scavenging activities and Cu2+-chelation ability of deer blood hydrolysate (DBH) significantly enhanced with increasing hydrolysis time (P < 0.05). The antioxidant activity of DBH, indicated by thiobarbituric acid-reactive substance (TBARS) values in a liposome-oxidizing system, increased with increasing DH (P < 0.05). The results indicated that antioxidant activity of DBH depended on hydrolysis time, and the hydrolyzed deer blood could be a potent food antioxidant.

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Flavonoids from aerial part of Algerian Ajuga iva (L.) schreb.: The HPLC-UV analysis and Antioxidant capacity

Kragujevac Journal of Science ◽

10.5937/kgjsci2143023b ◽

2021 ◽

pp. 23-34

Author(s):

Fadhela Boukada ◽

Boumediene Meddah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Capacity ◽

Aerial Part ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Dry Extract ◽

Antioxidant Agents ◽

Gallic Acid Equivalent

The study deals with the evaluation of the antioxidant capacity of extracts from the aerial part of Algerian Ajuga iva. Extraction of flavonoids was carried out by 85% of methanol, then the crude extract was successively separated with ethyl acetate, butanol, and water. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl, reducing power, and thiobarbituric acid reacting substances assays. Extracts are subject to HPLC-UV analysis. The average total phenol contents of extracts vary between 3.87 ± 0.17 and 149.74 ± 3.94 (gallic acid equivalent per gram of dry extract). Furthermore, tested extracts exhibited a broad range of flavonoid contents varying from 1.54 ± 0.09 to 41.18 ± 1.03 (catechin equivalent per gram of dry extract). Butanol and ethyl acetate fractions displayed the highest antioxidant activity. A good correlation between the phenolic and flavonoid contents and the antioxidant activity was observed. Rutin, caffeic acid, quercetin, p-coumaric acid, luteolin, and cinnamic acid were present in the extracts. The plant could be a potential source of antioxidant agents.

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Aktivitas Antioksidan Hidrolisat Kolagen Kulit Ikan Nila (Oreochromis niloticus)

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v23i3.31732 ◽

2020 ◽

Vol 23 (3) ◽

pp. 423-433

Author(s):

Deny Tri Prastyo ◽

Wini Trilaksani ◽

Nurjanah

Keyword(s):

Antioxidant Activity ◽

Oreochromis Niloticus ◽

Biological Activities ◽

Raw Material ◽

Antioxidant Compounds ◽

Degree Of Hydrolysis ◽

Hydrolysis Time ◽

Average Percentage ◽

Skin Collagen

Tilapia skin is a by-product of fish processing which can be used as an alternative raw material for collagen and its hydrolyzate. Collagen hydrolyzate is known to have potential biological activities including antioxidants. This study aims to examine the antioxidant activity of collagen hydrolyzate from the skin of tilapia (Oreochromis niloticus) in vitro. This research was divided into three stages including collagen pretratment, collagen extraction and collagen hydrolysis. In the hydrolysis process papain enzymes are used with different concentrations (4,000 U/g, 6,000 U/g, 8,000 U/g samples) with hydrolysis time for 60, 120 and 180 minutes. The average yield value of tilapia skin collagen hydrolyzate was 15.17%. The hydrolysis time, the concentration of enzymes given and the interaction between the two factors significantly affected the value of the degree of hydrolysis and antioxidant activity of the hydrolyzed collagen of tilapia skin. Collagen hydrolyzed for 120 minutes by giving an enzyme concentration of 8,000 U had an average percentage of degree of hydrolysis of 33.94%, and the best IC50 value was 93.32 µg/mL, categorized as strong antioxidant compounds. The zeta potential value of the tilapia skin collagen hydrolyzate was -10.9 mV.

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Physicochemical Properties and Biological Activity of Active Films Based on Corn Peptide Incorporated Carboxymethyl Chitosan

Coatings ◽

10.3390/coatings11050604 ◽

2021 ◽

Vol 11 (5) ◽

pp. 604

Author(s):

Liyan Wang ◽

Liang Lei ◽

Kang Wan ◽

Yuan Fu ◽

Hewen Hu

Keyword(s):

Antioxidant Activity ◽

Physicochemical Properties ◽

Food Packaging ◽

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Carboxymethyl Chitosan ◽

Vapor Permeability ◽

Zone Method ◽

Oil Resistance

Active films based on carboxymethyl chitosan incorporated corn peptide were developed, and the effect of the concentration of corn peptide on films was evaluated. Physicochemical properties of the films, including thickness, opacity, moisture content, color, mechanical properties, water vapor permeability, and oil resistance, were measured. Biological activities of the films, including the antioxidant and antibacterial activities, were characterized in terms of 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging activity, reducing power, the total antioxidant activity, and the filter disc inhibition zone method. The results indicated that the incorporation of corn peptide caused interactions between carboxymethyl chitosan and corn peptide in Maillard reaction and gave rise to the films light yellow appearance. Compared with the Control, the degree of glycosylation, browning intensity, thickness, opacity, tensile strength, antioxidant activity, and antibacterial activity of films were increased, but the elongation, vapor permeability, and oil resistance of films were decreased. The films based on corn peptide and carboxymethyl chitosan can potentially be applied to food packaging.

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Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation

Plants ◽

10.3390/plants10122669 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2669

Author(s):

Michaela Barkociová ◽

Jaroslav Tóth ◽

Katarzyna Sutor ◽

Natalia Drobnicka ◽

Slawomir Wybraniec ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Food Industry ◽

Biological Activities ◽

Ornamental Plants ◽

Reducing Power ◽

Activity Assay ◽

Natural Pigment ◽

E Coli ◽

Ft Ir

Epiphyllum, Hylocereus, and Opuntia plants belong to the Cactaceae family. They are mostly known as ornamental plants but also for their edible fruits, which can potentially be sources of betalains, such as betanin, a natural pigment used in the food industry, e.g., under the European label code E 162. The aim of this work was the identification of betalains (using LC-MS/MS), evaluation of total betalain content (spectrophotometrically), analysis of functional groups (using FT-IR), evaluation of antioxidant activity (using DPPH, ABTS, FRAP, DCFH-DA, and reducing power methods) and evaluation of antimicrobial activity (S. aureus, E. coli, and C. albicans) in fruits of Epiphyllum, Hylocereus, and Opuntia taxa. A total of 20 betalains were identified in the studied Cactaceae fruits. The Epiphyllum pink hybrid had the highest values of total betalains amongst all samples. The highest antioxidant activity was observed in the Epiphyllum pink hybrid, in Opuntia zacuapanensis and O. humifusa fruits. The antimicrobial activity assay showed that cacti fruits were not able to effectively inhibit the growth of E. coli, S. aureus, or C. albicans. Our results prove that these fruits are good sources of natural pigments—betalains. They do not contain toxic compounds in significant amounts and they exhibit antioxidant activity.

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Novel 1,2-thiazine-pyridine hybrid: Design, synthesis, antioxidant activity and molecular docking study

Letters in Drug Design & Discovery ◽

10.2174/1570180819666220106112650 ◽

2022 ◽

Vol 19 ◽

Author(s):

Rania B. Bakr ◽

Nadia A.A. Elkanzi

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Docking Study ◽

Molecular Docking Study ◽

Sod Activity ◽

Design Synthesis ◽

In Silico Docking

Background & objectives: 1,2-thiazine and pyridine heterocycles drew much attention due to their biological activities including antioxidant activity. Based upon fragment based drug design, novel pyrido[1,2]thiazines 9a-c, thiazolidinopyrido[1,2]thiazines 10a-c and azetidinopyrido[1,2]thiazines 11a-c were designed and prepared. Methods: These novel derivatives 9a-c, 10a-c and 11a-c were subjected to screening for their antioxidant activity via various assays as DPPH radical scavenging potential, reducing power assay and metal chelating potential. Results: All the assayed derivatives exhibited excellent antioxidant potential and the tested compounds 9a, 9b, 10a, 10b, 11a and 11b exhibited higher DPPH scavenging potential (EC50 = 32.7, 53, 36.1, 60, 40.6 and 67 µM, respectively) than ascorbic acid (EC50 = 86.58 µM). While targets 9a, 10a and 11a (RP50 = 52.19, 59.16 and 52.25 µM, respectively) exhibited better reducing power than the ascorbic acid (RP50 = 84.66 µM). Computational analysis had been utilized to prophesy the bioactivity and molecular properties of the target compounds. Conclusion: To predict the binding manner of the novel derivatives as antioxidants, in-silico docking study had been performed to all the newly prepared compounds inside superoxide dismutase (SOD) and catalase (CAT) active site. The most active antioxidant candidate 9a (EC50 = 32.7 µM, RP50 = 52.19 µM) displayed excellent binding with Lys134 amino acid residing at Cu-Zn loop of SOD with binding energy score = -7.54 Kcal/mol thereby increase SOD activity and decrease reactive oxygen species.

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Isolation and Biological Activities of Citral from Sweet Orange Oil

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.750-752.1621 ◽

2013 ◽

Vol 750-752 ◽

pp. 1621-1625 ◽

Cited By ~ 1

Author(s):

Xiao Lin Xu ◽

Qing Zhu ◽

Hui Lv ◽

Wen Hui Wu ◽

Min Lv ◽

...

Keyword(s):

Antioxidant Activity ◽

Sweet Orange ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Human Cell Line ◽

Orange Oil ◽

Cell Line Hela ◽

Ic50 Values

The purpose of this study was to isolate citral from sweet orange oil by combined usage of molecular distillation and column chromatography. Additionally, the antioxidant activity (by DPPH radical scavenging activity and reducing power) of citral was evaluated. A significant and linear correlation coefficient between the antioxidant activity and the concentration of citral was found (R2=0.9925). On the other hand, the cytotoxic effects of the citral against tumoral human cell line Hela was examined by MTT assay and the IC50 values was 32.56 μg/mL. Overall, results presented here suggest that the citral possesses antioxidant activity and cytotoxic properties, and is a potential source of active ingredients for food and pharmaceutical industry.

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Biological Activities of the Polysaccharides Produced in Submerged Culture of Two EdiblePleurotus ostreatusMushrooms

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/565974 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

Emanuel Vamanu

Keyword(s):

Antioxidant Activity ◽

Pleurotus Ostreatus ◽

Submerged Culture ◽

Biological Activities ◽

Reducing Power ◽

Scavenging Activity ◽

Carbohydrate Analysis ◽

Chelating Activity ◽

Batch System

Exopolysaccharides (EPS) and internal (intracellular) polysaccharides (IPS) obtained from thePleurotus ostreatusM2191 and PBS281009 cultivated using the batch system revealed an average of between 0.1–2 (EPS) and 0.07–1.5 g/L/day (IPS). The carbohydrate analysis revealed that the polysaccharides comprised 87–89% EPS and 68–74% IPS. The investigation of antioxidant activityin vitrorevealed a good antioxidant potential, particularly for the IPS and EPS isolated from PBS281009, as proved by the EC50value for DPPH, ABTS scavenging activity, reducing power, and iron chelating activity.

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In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-3-408 ◽

2009 ◽

Vol 64 (3-4) ◽

pp. 197-200 ◽

Cited By ~ 9

Author(s):

Hari Datta Bhattarai ◽

Babita Paudel ◽

Hong Kum Lee ◽

Hyuncheol Oh ◽

Joung Han Yim

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Reducing Power ◽

Commercial Application ◽

Moss Species ◽

Antioxidant Capacities ◽

Antioxidant Agents ◽

Antarctic Moss

Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe3+) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application.

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Effect of pepsin hydrolysis on antioxidant activity of jellyfish protein hydrolysate

E3S Web of Conferences ◽

10.1051/e3sconf/202130202010 ◽

2021 ◽

Vol 302 ◽

pp. 02010

Author(s):

Pratchaya Muangrod ◽

Wiriya Charoenchokpanich ◽

Vilai Rungsardthong ◽

Savitri Vatanyoopaisarn ◽

Benjamaporn Wonganu ◽

...

Keyword(s):

Antioxidant Activity ◽

Protein Hydrolysate ◽

Inhibitory Effect ◽

Raw Material ◽

Degree Of Hydrolysis ◽

Dpph Radical ◽

Radical Scavengers ◽

Hydrolysis Time ◽

Hydrolysis Of ◽

By Products

Edible jellyfish have been consumed as food for more than a century with offering high protein and crunchy texture. The pepsin hydrolysis of jellyfish protein yields jellyfish protein hydrolysate (ep-JPH), reported for potential bioactivities such as antioxidant activity or antihypertensive activities. Due to the substantial number of by-products generated from jellyfish processing, the by-products were then selected as a raw material of JPH production. This research aimed to evaluate the effect of the hydrolysis time of pepsin on the antioxidant activity of ep-JPH. The dried desalted jellyfish by-products powder was enzymatically hydrolysed by 5% (w/w) pepsin, and the hydrolysis time was varied from 6, 12, 18, and 24 h at 37oC. Results showed that increased hydrolysis time increased the degree of hydrolysis (DH) and inhibition of DPPH radical. The 24 h ep-JPH possessed the highest DH and the highest inhibitory effect of DPPH radical. The results demonstrated that, in this experiment, all ep-JPHs were DPPH radical scavengers, exhibiting different inhibition activities depending on DH values.

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