The Optimization Study on Solid Culture Medium of North Cordyceps

2013 ◽  
Vol 781-784 ◽  
pp. 884-888
Author(s):  
Xiu Li Qin ◽  
De Ming Han
Keyword(s):  
Growth Rate ◽  
Nutrient Solution ◽  
Wheat Bran ◽  
Culture Medium ◽  
Orthogonal Test ◽  
Cordyceps Sinensis ◽  
Solid Matrix ◽  
Solid Culture ◽  
The North ◽  
Solid Culture Medium

Artificial cultivation of solid culture medium of North Cordyceps was optimized in this experiment. The results show that, the North Cordyceps can be cultivated in many types of solid culture medium, the growth rate and density of the hyphae was highest in a single solid culture medium with rice as the solid substrate, but adding 10%-15% wheat bran in rice solid culture medium, the growth rate and density of the hyphae is better than the single matrix of rice; the suitable nutrient solution was determined by contrast test and L9 (34) orthogonal test, the composition was sucrose 2%, yeast extract 1.5%, MgSO40.1%, KH2PO4 0.1%; the suitable solid culture medium of the North Cordyceps sinensis was determined by the L9 (33) orthogonal test, the composition was rice 85%, wheat bran 15%, pH 5.5-6.5, the ratio of solid and water ( solid matrix / nutrient solution )was 1:1.5.

scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

Intracellular Biosynthesis of Fluorescent CdSe Quantum Dots inBacillus subtilis:A Strategy to Construct Signaling Bacterial Probes for Visually Detecting Interaction BetweenBacillus subtilisandStaphylococcus aureus

2015 ◽  
Vol 22 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Zheng-Yu Yan ◽  
Xiao-Xia Ai ◽  
Yi-Long Su ◽  
Xin-Ying Liu ◽  
Xiao-Hui Shan ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Culture Medium ◽  
Imaging Mass Spectrometry ◽  
Cdse Quantum Dots ◽  
Mass Spectrometry Detection ◽  
New Paradigm ◽  
Solid Culture ◽  
Bacterial Interaction ◽  
Solid Culture Medium ◽  
Good Uniformity

AbstractIn this work, fluorescentBacillus subtilis(B. subtilis) cells were developed as probes for imaging applications and to explore behaviorial interaction betweenB. subtilisandStaphylococcus aureus(S. aureus). A novel biological strategy of coupling intracellular biochemical reactions for controllable biosynthesis of CdSe quantum dots by livingB. subtiliscells was demonstrated, through which highly luminant and photostable fluorescentB. subtiliscells were achieved with good uniformity. With the help of the obtained fluorescentB. subtiliscells probes,S. aureuscells responded to co-culturedB. subtilisand to aggregate. The degree of aggregation was calculated and nonlinearly fitted to a polynomial model. Systematic investigations of their interactions implied thatB. subtiliscells inhibit the growth of neighboringS. aureuscells, and this inhibition was affected by both the growth stage and the amount of surroundingB. subtiliscells. Compared to traditional methods of studying bacterial interaction between two species, such as solid culture medium colony observation and imaging mass spectrometry detection, the procedures were more simple, vivid, and photostable due to the efficient fluorescence intralabeling with less influence on the cells’ surface, which might provide a new paradigm for future visualization of microbial behavior.

scholarly journals STUDIES ON CERTAIN GROWTH PARAMETERS OF DIFFERENT PROBIOTIC STRAINS

2017 ◽  
Vol 23 (4) ◽  
pp. 19-24
Author(s):  
DUMITRA RĂDUCANU ◽  
ANA-MARIA GEORGESCU
Keyword(s):  
Nutrient Medium ◽  
Growth And Development ◽  
Culture Medium ◽  
Growth Parameters ◽  
Probiotic Bacteria ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Probiotic Strains ◽  
Direct Counting ◽  
Better Than

The researchers found that probiotics contain microorganisms belonging to genus: Streptococcus, Lactobacillus, Bacillus, Aspergillus, Saccharomyces, Enterococcus, Pediococcus, enzymes (lactoperoxidase, gluconase, nonspecific enzymes) and rumen extracts. In this study, commercial probiotic bacteria known as "Linex" were used as samples. Cultural characteristics of these probiotic bacteria have been isolated and studied. It has been found that solid culture medium (nutritional gelose) favored the growth and development of bifidobacteria better than the liquid nutrient medium (nutrient broth). Thus, the number of bifidobacteria resulting from direct counting with Thoma chamber was of 7890 cells.

scholarly journals Cultivation and growth dynamics of endophytic fungi in a solid culture medium based on sugar beet pulp

2019 ◽  
Vol 100 (1) ◽  
pp. 441-446 ◽  
Author(s):  
Beatriz R Vázquez‐de‐Aldana ◽  
María José Cuesta ◽  
Iñigo Zabalgogeazcoa
Keyword(s):  
Sugar Beet ◽  
Endophytic Fungi ◽  
Culture Medium ◽  
Growth Dynamics ◽  
Sugar Beet Pulp ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Beet Pulp

Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

2021 ◽  
pp. 113-120
Author(s):  
A.L. Arruda ◽  
F.R. Nerbass ◽  
A.A. Kretzschmar ◽  
L. Rufato ◽  
A.J. Posser ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Raman Spectroscopic Method for Identification of Clinically Relevant Microorganisms Growing on Solid Culture Medium

2000 ◽  
Vol 72 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Kees Maquelin ◽  
Lin-P'ing Choo-Smith ◽  
Tamara van Vreeswijk ◽  
Hubert Ph. Endtz ◽  
Brian Smith ◽  
...  
Keyword(s):  
Culture Medium ◽  
Spectroscopic Method ◽  
Solid Culture ◽  
Raman Spectroscopic ◽  
Solid Culture Medium

scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Bazı Turunçgil Anaçlarının Klasik ve Yeni Nesil Doku Kültürü Teknikleri ile Mikroçoğaltımı

2019 ◽  
Vol 7 (9) ◽  
pp. 1469
Author(s):  
Melike Cengiz ◽  
Yıldız Aka Kaçar
Keyword(s):  
Culture Medium ◽  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Solid Culture ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Rooting Medium ◽  
Solid Culture Medium ◽  
Isopentenyl Adenine ◽  
Bioreactor System

In this study, micropropagation and rooting of ‘Tuzcu 31-31 sour orange’ and ‘C-35 citrange’ citrus rootstocks were conducted by comparing with Plantform temporary immersion bioreactor system and traditional solid culture. Murashige and Skoog Medium (MS) and Woody Plant Medium (WPM) supplemented with 6-Benzylaminopurine (BAP) (0, 1.0, 2.0 mg L-1), Kinetin (KIN) (0, 0.5, 1.0 mg L-1) and 2-Isopentenyl adenine (2IP) (0, 1.0, 2.0 mg L-1) were used in solid culture experiments. For solid culture rooting experiments, MS, ½ MS and WPM media supplemented with different concentrations of 1-Naphthaleneacetic acid (NAA) (0, 0.5, 1.0, 2.0 mg L-1) and Indole-3-butyric acid (IBA) (0, 0.5, 1.0, 2.0 mg L-1) were used. In both genotypes, the best micropropagation and rooting results were obtained from MS medium containing 2.0 mg L-1 BAP and ½ MS nutrient medium containing 0.5 mg L-1 NAA, respectively. Plantform bioreactor system was studied with the best medium content determined for micropropagation and rooting. As a result of the study, Plantform system gave better results in terms of plant quality in the micropropagation medium for both genotypes. Plantform system in rooting medium was found to be more advantageous than solid culture medium. As a result of the screening with SSR markers, it was determined that there was no somaclonal variation in the plants micropropagated and rooted in Plantform system.

scholarly journals Revisiting the susceptibility testing of Mycobacterium tuberculosis to ethionamide in solid culture medium

2015 ◽  
Vol 142 (5) ◽  
pp. 538 ◽  
Author(s):  
Vanaja Kumar ◽  
Rajagopalan Lakshmi ◽  
Ranjani Ramachandran ◽  
DRavi Kumar ◽  
ASyam Sundar ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Culture Medium ◽  
Susceptibility Testing ◽  
Solid Culture ◽  
Solid Culture Medium
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