The Application of Phytotesting Method to Assess the Toxicity of Titanium Etching Effluents

2018 ◽  
Vol 769 ◽  
pp. 161-165 ◽  
Author(s):  
Nikolay Alekseevich Bykovsky ◽  
Timur Z. Zabirov ◽  
Inna V. Ovsyannikova ◽  
Nadezhda F. Fanakova
Keyword(s):  
Average Length ◽  
Ground Surface ◽  
Dry Weight ◽  
Sewage Water ◽  
Lepidium Sativum ◽  
Acid Etching ◽  
Dilution Factor ◽  
Etching Solution ◽  
Acute Toxic Effect ◽  
Chemical Solutions

The article evaluates the toxicity of the spent acid etching solution (SAES) formed in production of titanium products when etched with a mixture of hydrofluoric and hydrochloric acids. The SAES contained TiF3, HF and HCl in the amount of 21.9 g/l, 1.7 g/l and 6.2 g/l, respectively. To determine the toxicity of SAES the phytotesting method was used. As a phytoecological indicator, a cress of Zabava variety was used. The experiment was carried out according to the method for determining the toxicity of drinking, ground, surface and sewage water, chemical solutions by measuring the germination index, average length and average dry weight of seedlings of cress seeds (Lepidium sativum). The SAES toxicity was determined before its neutralization with alkali and after neutralization with alkali. It is shown that SAES has an acute toxic effect both before neutralization and after neutralization with alkali. To determine the safe dilution factor, the effect of SAES dilution on seed germination, average length and average dry weight of seedlings was investigated. It was found that “seedlings average length – dilution factor” equations most reliably describe the experimental data. The safe dilution factor calculated from these dependencies is 669.2 for non-neutralized SAES and 382.5 for neutralized SAES.

scholarly journals Toxicity assessment of pickling liquors containing TiF₃, HF and HCl before and after neutralization using a phytotesting method

2017 ◽  
Vol 6 (2) ◽  
pp. 24-27
Author(s):  
Nikolay Alekseevich Bykovsky ◽  
Timur Zulfukarovich Zabirov ◽  
Inna Vyacheslavovna Ovsyannikova ◽  
Lyudmila Nikolaevna Puchkova ◽  
Nadezhda Nikolaevna Fanakova
Keyword(s):  
Dilution Rate ◽  
Average Length ◽  
Ground Surface ◽  
Dry Weight ◽  
Sewage Water ◽  
Toxicity Assessment ◽  
Rate Equations ◽  
Lepidium Sativum ◽  
Before And After ◽  
Acute Toxic Effect

The paper evaluates the toxicity of spent acid pickling liquor (SAPL) formed in the production of titanium products during its etching with a mixture of hydrofluoric and hydrochloric acids. The SAPL contained TiF₃, HF and HCl in the amounts of 21,9 g/l, 1,7 g/l and 6,2 g/l, respectively. To determine the toxicity of SAPL, a phytotesting method was used. As a phytoecological indicator, cress of Zabava variety was used. The experiment was carried out according to the procedure for determining the toxicity of drinking, ground, surface and sewage water; the toxicity of chemical solutions by measuring the germination index, average length and average dry weight of seed germs of cress (Lepidium sativum). The toxicity of the SAPL was determined before and after neutralizing with alkali. It has been shown that SAPL has an acute toxic effect both before and after its neutralization by alkali. To determine a safe dilution rate, the influence of SAPL dilution on seed germination, average length and average dry weight of the seedlings was studied. It was found that the seedlings average length-dilution rate equations most reliably describe the experimental findings. The safe dilution rate calculated from these relations is 669,2 for non-neutralized SAPL and 382,5 for alkaline neutralized SAPL.

Toxicity Assessment of the Main Waste of Soda Production by Phytotesting

2018 ◽  
Vol 769 ◽  
pp. 166-171
Author(s):  
Nikolay Alekseevich Bykovsky ◽  
Inna V. Ovsyannikova ◽  
Lyudmila N. Puchkova ◽  
Nadezhda N. Fanakova
Keyword(s):  
Seed Germination ◽  
Toxic Effect ◽  
Plant Species ◽  
Average Length ◽  
Dry Weight ◽  
Toxicity Assessment ◽  
Regression Equations ◽  
Dilution Factor ◽  
Acute Toxic Effect ◽  
Soda Ash

The paper investigates the use of cress (Zabava and Krupnolistovoi varieties) and wheat (Salavat Yulaev and Omskaya varieties) as phytoecological indicators. The object for testing was still waste liquid, the main waste of soda ash production. The toxicity was assessed by three parameters (seed germination, length and dry weight of the seedlings). It is shown that the still waste liquid has an acute toxic effect on all phytoecological indicators used. It has been found that the regression equations for seed germination and dry weight of seedlings in contrast to their average length for all plants do not adequately describe the experimental results and can not be used to evaluate the safe dilution factor. The safe dilution factor, determined by the average length of the seedlings, for cress Zabava is 34.7 and cress Krupnolistovoi is 34.4, and for wheat Salavat Yulaev is 73.0 and for wheat Omskaya is 81.8. It was established that the safe dilution factor determined with the use of different plant species varies quite strongly.

scholarly journals Wastewater production of ethylenediamine and the assessment of their toxicity by phytotesting

2019 ◽  
Vol 8 (1) ◽  
pp. 21-26
Author(s):  
Nikolai Alekseevich Bykovsky ◽  
Evgeny Abramovich Kantor ◽  
Lyudmila Nikolaevna Puchkova ◽  
Nadezhda Nikolaevna Fanakova ◽  
Vadim Stanislavovich Fanakov
Keyword(s):  
Seed Germination ◽  
Toxic Effect ◽  
Average Length ◽  
Dry Weight ◽  
Experimental Results ◽  
Regression Equations ◽  
Nacl Solution ◽  
First Case ◽  
Acute Toxic Effect ◽  
Ethylenediamine Dihydrochloride

This paper discusses occurrence of toxic wastewater from the production of ethylenediamine by aminating 1,2-dichloroethane with ammonia. It is shown that wastewater from ethylenediamine production is formed at the stages of evaporation of ethylenediamine dihydrochloride and rectification of a mixture of amines obtained as a result of evaporation. In the first case, the wastewater contains a saturated NaCl solution with a content of 1 2% polyethylene polyamines, and in the second case, the drain contains a solution containing about 1% of ethylenediamine and about 2% of ammonia. To study the toxicity of these wastewater, watercress of Zabava and Krupnolistovoy varieties were used. The assessment of toxic properties of wastewater was carried out according to such values as seed germination, the average length of seedlings and the dry weight of seedlings. It is revealed that the studied drains have an acute toxic effect on the watercress of both varieties. It is shown that the regression equations obtained for the dry weight of seedlings and seed germination, in contrast to the length of the seedlings, do not adequately describe the experimental results and cannot be used to determine the safe multiplicity of breeding. The safe multiplicity of breeding, calculated using the average length of seedlings, ranges from 489,1 to 892,9 for various drains and watercress varieties. It is shown that the most toxic is the runoff containing a saturated NaCl solution with a content of 1 2% of polyethylene polyamines Zabava is the most sensitive to the degree of toxicity of runoff.

scholarly journals Phytotoxic effects of Cerbera manghas L. leaf extracts on seedling elongation of four monocot and four dicot test species

2017 ◽  
Vol 70 (3) ◽  
Author(s):  
Ichsan Nurul Bari ◽  
Hisashi Kato-Noguchi
Keyword(s):  
Weed Management ◽  
Dry Weight ◽  
Phleum Pratense ◽  
Italian Ryegrass ◽  
Lepidium Sativum ◽  
Leaf Extracts ◽  
Isolation And Identification ◽  
Test Species ◽  
Phytotoxic Effects ◽  
Cerbera Manghas

Exploration of allelochemicals with phytotoxic effects is intended to minimize a current dependency on synthetic herbicides in weed management. Several allelochemicals from the tropical tree <em>Cerbera manghas</em> (sea mango) have been reported as termiticides and bactericides. The present study investigated possible phytotoxic effects of <em>C. manghas</em> leaf extracts under laboratory conditions. Four monocots: barnyard grass (<em>Echinochloa crus-galli</em>), foxtail fescue (<em>Vulpia myuros</em>), Italian ryegrass (<em>Lolium multiflorum</em>), and timothy (<em>Phleum pratense</em>) and four dicots: alfalfa (<em>Medicago sativa</em>), garden cress (<em>Lepidium sativum</em>), lettuce (<em>Lactuca sativa</em>), and rapeseed (<em>Brassica napus</em>) were used as test species. Elongation of both shoots and roots of seedlings was measured to assess any phytotoxic effects. The results showed that the sensitivities of shoots and roots were different between the test species, and the inhibition of seedling elongation significantly increased with increasing concentration of leaf extracts of <em>C. manghas</em> for all the test species. The IC<sub>50</sub> (50% inhibitory concentration) values showed that 8.50–32.30 and 4.26–34.67 mg dry weight equivalent extract mL<sup>−1</sup> of <em>C. manghas</em> inhibited seedling elongation by 50%, for shoots and roots respectively. Isolation and identification of the phytotoxic substances from <em>C. manghas</em> are suggested for future investigation.

The Effect of Sodium Fluoride in Acid Etching Solution on Sealant Bond and Fluoride Uptake

1980 ◽  
Vol 59 (3) ◽  
pp. 625-630 ◽  
Author(s):  
Yoshikazu Takahashi ◽  
Yukio Arakawa ◽  
Takashi Matsukubo ◽  
Mitsuharu Takeuchi
Keyword(s):  
Sodium Fluoride ◽  
Acid Etching ◽  
Etching Solution ◽  
Fluoride Uptake

scholarly journals Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L.

2019 ◽  
Vol 20 (7) ◽  
pp. 1787 ◽  
Author(s):  
Muhammad Asad Ullah ◽  
Duangjai Tungmunnithum ◽  
Laurine Garros ◽  
Samantha Drouet ◽  
Christophe Hano ◽  
...  
Keyword(s):  
Callus Culture ◽  
High Performance ◽  
Biological Activities ◽  
Fold Increase ◽  
Dry Weight ◽  
Callus Cultures ◽  
Lepidium Sativum ◽  
Uv C ◽  
Lepidium Sativum L

Lepidium sativum L. is a rich source of polyphenols that have huge medicinal and pharmaceutical applications. In the current study, an effective abiotic elicitation strategy was designed for enhanced biosynthesis of polyphenols in callus culture of L. sativum. Callus was exposed to UV-C radiations for different time intervals and various concentrations of melatonin. Secondary metabolites were quantified by using high-performance liquid chromatography (HPLC). Results indicated the total secondary metabolite accumulation of nine quantified compounds was almost three fold higher (36.36 mg/g dry weight (DW)) in melatonin (20 μM) treated cultures, whereas, in response to UV-C (60 min), a 2.5 fold increase (32.33 mg/g DW) was recorded compared to control (13.94 mg/g DW). Metabolic profiling revealed the presence of three major phytochemicals, i.e., chlorogenic acid, kaemferol, and quercetin, in callus culture of L. sativum. Furthermore, antioxidant, antidiabetic, and enzymatic activities of callus cultures were significantly enhanced. Maximum antidiabetic activities (α-glucosidase: 57.84%; α-amylase: 62.66%) were recorded in melatonin (20 μM) treated callus cultures. Overall, melatonin proved to be an effect elicitor compared to UV-C and a positive correlation in these biological activities and phytochemical accumulation was observed. The present study provides a better comparison of both elicitors and their role in the initiation of physiological pathways for enhanced metabolites biosynthesis in vitro callus culture of L. sativum.

Adhesive Bonding of Various Materials to Hard Tooth Tissues. XIV. Enamel Mordant Selection Assisted by ESCA (XPS)

1978 ◽  
Vol 57 (4) ◽  
pp. 551-556 ◽  
Author(s):  
R.L. Bowen
Keyword(s):  
Chemical Analysis ◽  
Phosphoric Acid ◽  
Surface Analysis ◽  
Photoelectron Spectroscopy ◽  
Electron Spectroscopy ◽  
Adhesive Bonding ◽  
Acid Etching ◽  
Etching Solution ◽  
X Ray

Surface analysis by XPS (X-ray photoelectron spectroscopy), also called ESCA (electron spectroscopy for chemical analysis), indicates that only certain cations are appreciably sorbed by enamel from an acid etching solution containing phosphoric acid and equimolar concentrations of candidate mordant salts.

scholarly journals The molecular structure of human tissue type XV presents a unique conformation among the collagens

2007 ◽  
Vol 404 (3) ◽  
pp. 535-544 ◽  
Author(s):  
Jeanne C. Myers ◽  
Peter S. Amenta ◽  
Arnold S. Dion ◽  
Justin P. Sciancalepore ◽  
Chandrasekaran Nagaswami ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Average Length ◽  
Dry Weight ◽  
Molecular Shape ◽  
Tissue Type ◽  
Transmission Electron ◽  
Fibrillar Collagens ◽  
Umbilical Cords ◽  
Collagenous Domain ◽  
Order Structures

Establishing the structure of the non-fibrillar collagens has provided a unique perspective to understanding their specialized functions in the extracellular matrix. These proteins exhibit very diverse conformations and supramolecular assemblies. Type XV collagen is a large macromolecule distinguished by a highly interrupted collagenous domain and many utilized sites of attachment for CS (chondroitin sulfate) and HS (heparan sulfate) glycosaminoglycan chains. It is present in most basement membrane zones of human tissues, where it is found closely associated with large collagen fibrils. To determine the molecular shape and organization of type XV, the protein was purified from human umbilical cords by salt extraction, and by ion-exchange and antibody-affinity chromatography. The representation of type XV in one of its most abundant tissue sources is estimated at only (1–2)×10−4% of dry weight. The molecules examined by transmission electron microscopy after rotary shadowing were visualized in multiple forms. Relatively few type XV monomers appeared elongated and kinked; most molecules were found in a knot/figure-of-eight/pretzel configuration not previously described for a collagen. Collective measurements of these populations revealed an average length of 193±16 nm. At the N-terminal end, identified by C-terminal antibody binding, were three 7.7 nm-diameter spheres, corresponding to TSPN-1 (N-terminal module of thrombospondin-1) modules, and attached to the collagen backbone by a short linker. The type XV monomers show the ability to self-assemble into higher-order structures. Some were arranged in complex clusters, but simpler oligomers, which may represent intermediates, were observed in a cruciform pattern with intermolecular binding sites that probably originate in the interruption sequences. The morphology of type XV is thus the antithesis of the fibrillar collagens, and the shape attains the required flexibility to form the spectrum of interconnecting links between banded fibrils at the basement membrane/interstitial border. These type XV structures may act as a biological ‘spring’ to stabilize and enhance resilience to compressive and expansive forces, and the multimers, in particular, with selective complements of many localized CS and HS chains, may be instrumental in spatial and temporal recruitment of modulators in growth, development and pathological processes.

A morphological and biochemical analysis of embryo maturation during yellow cypress seed development

1994 ◽  
Vol 24 (3) ◽  
pp. 431-441 ◽  
Author(s):  
Mimi L. Kurz ◽  
Dane R. Roberts ◽  
Barry S. Flinn ◽  
William E. Vidaver
Keyword(s):  
Seed Dispersal ◽  
Seed Development ◽  
Storage Proteins ◽  
Early Stage ◽  
Average Length ◽  
Dry Weight ◽  
Moisture Loss ◽  
Embryo Maturation ◽  
Molecular Masses ◽  
Stage 1

Embryo maturation in yellow cypress (Chamaecyparisnootkatensis (D. Don) Spach) seeds was studied at the morphological, anatomical, and biochemical level over the second and third seasons of seed development. Monthly cone collections were made from six open-pollinated families from two seed orchards. The average number of seeds and embryos per cone for each family was 8.8 and 4.6, respectively. Sixty to 70% of those embryos harvested from early collection dates (December 1988–March 1989) were precotyledonary (stages 1 and 2), with an average length of 0.5 mm. By April 1989 a distinct shift in the proportion of precotyledonary (stages 1 and 2) to late cotyledonary (stages 5 and 6) embryos was observed. The frequency of late cotyledonary (stages 5 and 6) embryos was 100% by August 1989 of the third growing season and averaged 2.5 mm in overall size. Moisture loss and dry weight accumulation were still progressing at the time of seed dispersal and may suggest embryo immaturity. Anatomical studies revealed protein and lipid bodies were present as early as the stage 1 embryo and became increasingly abundant during maturation. The major storage proteins isolated from protein bodies of embryo tissues had apparent molecular masses of 37, 36, 35, 34, 28, 26, and 24 kDa. Based on their solubility characteristics, the proteins were identified as buffer-soluble albumins that contained intermolecular disulfide linkages. Storage proteins were detected in early stage embryos and increased in abundance throughout maturation. Upon germination, storage proteins slowly declined to low levels by 16 and 20 days postgermination. The evidence suggests that yellow cypress embryos may be immature at the time of seed dispersal and this immaturity may contribute to the poor germination exhibited by this species.

scholarly journals Timing of Macronutrient Supply during Cutting Propagation of Petunia

HortScience ◽  
2011 ◽  
Vol 46 (3) ◽  
pp. 475-480 ◽  
Author(s):  
Kathryn M. Santos ◽  
Paul R. Fisher ◽  
Thomas Yeager ◽  
Eric H. Simonne ◽  
Hannah S. Carter ◽  
...  
Keyword(s):  
Nutrient Uptake ◽  
Vegetative Propagation ◽  
Growth And Development ◽  
Average Length ◽  
Dry Weight ◽  
Water Soluble ◽  
Time Intervals ◽  
Primary Roots ◽  
Soluble Fertilizer ◽  
Micronutrient Fertilizer

The objective was to quantify the effect of the timing of macronutrient applications on nutrient uptake, growth, and development of Petunia ×hybrida Hort. Vilm.-Andr. ‘Supertunia Royal Velvet’ during vegetative propagation. Starting with unrooted cuttings (Day 0), fertigation was applied continuously at three time intervals (Day 0 to 7, Day 8 to 14, or Day 15 to 21) using either a “complete” (C) water-soluble fertilizer containing (in mg·L−1) 75 NO3-N, 25 NH4-N, 12 phosphorus (P), 83 potassium (K), 20 calcium (Ca), 10 magnesium (Mg), 1.4 sulfur (S), 2 iron (Fe), 1 manganese (Mn), 1 zinc (Zn), 0.5 copper (Cu), 0.5 boron (B), and 0.2 molybdenum (Mo) or a micronutrient fertilizer (M) containing (in mg·L−1) 1.4 S, 2 Fe, 1 Mn, 1 Zn, 0.5 Cu, 0.5 B, and 0.2 Mo in a complete factorial arrangement. With constant fertigation using the C fertilizer, plant dry weight (DW) doubled from Day 0 (sticking of unrooted cuttings) to Day 7 (0.020 g to 0.047 g), root emergence was observed by Day 4, and by Day 7, the average length of primary roots was 2.6 cm. During any week that the M fertilizer was substituted for the C fertilizer, tissue N–P–K concentrations decreased compared with plants receiving the C fertilizer. For example, plants receiving the M fertilizer between Day 0 and 7 had 20% lower tissue-N concentration at Day 7 compared with those receiving the C fertilizer. Although both shoot DW and leaf count increased once macronutrient fertilization was resumed after Day 7, final shoot DW and leaf count were lower than plants receiving C fertilizer from Day 0 to 21. Time to first root emergence was unaffected by fertigation. Constant application of C resulted in a higher shoot-to-root ratio at Day 21 than all other treatments. Results emphasize the importance of early fertigation on petunia, a fast-rooting species, to maintain tissue nutrient levels within recommended ranges.

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