Porous Hydroxyapatite/Chitosan/Carboxymethyl Cellulose Scaffolds with Tunable Microstructures for Bone Tissue Engineering

2019 ◽  
Vol 819 ◽  
pp. 9-14 ◽  
Author(s):  
Kanharit Wongsawichai ◽  
Arada Kingkaew ◽  
Aninart Pariyaisut ◽  
Supang Khondee
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Carboxymethyl Cellulose ◽  
Cell Attachment ◽  
Biological Properties ◽  
Porous Hydroxyapatite ◽  
Porous Microstructure

Bone tissue engineering is an alternative approach to generate bone using biomaterials and cells. Hydroxyapatite (HA) has good biocompatibility, osteoinductivity, and osteoconductivity. However, it has limited utility due to poor mechanical properties and slow degradation rate. To improve mechanical properties and to modify degradation profile, hydroxyapatite was tethered in chitosan (CS) and carboxymethyl cellulose (CMC) complex. Gelatin was incorporated to promote cell attachment and polyvinyl alcohol (PVA) was used to improve mechanical strength of this scaffold. The physico-mechanical and biological properties of these scaffolds were investigated. Fourier transform infrared (FTIR) analysis and X-ray diffraction (XRD) showed the incorporation of hydroxyapatite in polymer matrix. The scaffolds had density, compressive strength, and Young’s modulus in the range of 0.24-0.30 g/cm3, 0.028-0.035 MPa, 0.178-0.560 MPa, respectively. The scaffolds had porosity of 69-91 percent. Higher content of PVA decreased porosity of scaffolds. Scanning electron microscope showed porous microstructure with pore size in the range of 60-183 μm. In vitro test on MC3T3-E1 preosteoblast cells showed negligible cytotoxicity of scaffolds. The data suggested that HA/CS/CMC/gelatin/PVA scaffold has potential applications in bone tissue engineering.

Biological evaluation of micro-patterned hyaluronic acid hydrogel for bone tissue engineering

2014 ◽  
Vol 86 (12) ◽  
pp. 1911-1922 ◽  
Author(s):  
Hyo Seung Park ◽  
Su Yeon Lee ◽  
Hyunsik Yoon ◽  
Insup Noh
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Cells ◽  
Cell Attachment ◽  
Biological Properties ◽  
Biological Evaluation ◽  
Micro Patterning

Abstract Design of micro-patterning of hydrogel is of critical importance in both understanding cellular behaviors and mimicking controlled microenvironments and architectures of diverse well-organized tissues. After micro-patterning of hyaluronic acid (HA) hydrogel on a poly(dimethyl siloxane) substrate, its physical and biological properties have been compared with those of a non-patterned hydrogel for its possible applications in bone tissue engineering. The micro-patterned morphologies of HA hydrogel in both swollen and dehydrated forms have been observed with light microscope and scanning electron microscope, respectively, before and after in vitro cell culture. When MC3T3 bone cells were in vitro cultured on both HA hydrogels, the micro-patterned one shows excellence in cell proliferation and lining for 7 days along the micro-pattern paths over those of the non-patterned one, which have shown less cell-adhesiveness. The cytotoxicity of the micro-patterned HA hydrogels was in vitro evaluated by the assays of MTT, BrdU and Neutral red. The viability and morphology of MC3T3 cells on both HA hydrogels were observed with a fluorescence microscope by the live & dead assay, where their viability was confirmed by staining of F-actin development. The results of their H&E staining showed that both micro-patterned and non-patterned hydrogels induced development of tissue regeneration as observed by cell attachment, proliferation, and survivability, but the micro-patterned one induced distinctive patterning of both better initial cells adhesion on the micro-patterns and subsequently development of their proliferation and extracellular matrix, which were considered as important characteristics in their applications to tissue engineering.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

2015 ◽  
Vol 3 (23) ◽  
pp. 4679-4689 ◽  
Author(s):  
Ya-Ping Guo ◽  
Jun-Jie Guan ◽  
Jun Yang ◽  
Yang Wang ◽  
Chang-Qing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Biological Properties ◽  
Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

Herbally Painted Biofunctional Scaffolds with Improved Osteoinductivity for Bone Tissue Engineering

2019 ◽  
Vol 41 ◽  
pp. 49-68 ◽  
Author(s):  
Shivaji Kashte ◽  
Gajanan Arbade ◽  
R.K. Sharma ◽  
Sachin Kadam
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Tensile Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Biological Properties ◽  
Osteogenic Potential ◽  
Composite Scaffolds ◽  
Alizarin Red

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) andCissusquadrangularis(CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21stday in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.

scholarly journals TiO2 doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast-like cell response for application in bone tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (68) ◽  
pp. 39768-39779
Author(s):  
Sarim Khan ◽  
Viney Kumar ◽  
Partha Roy ◽  
Patit Paban Kundu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Response ◽  
Halloysite Nanotubes ◽  
Biological Properties ◽  
Halloysite Nanotube ◽  
Nanotube Membranes ◽  
Hydroxyapatite Composite

This two-stage study aims to optimize the amount of halloysite nanotubes and TiO2 in a chitosan/nano-hydroxyapatite composite to tailor the mechanical and biological properties for application in bone tissue engineering.

scholarly journals Cuttlefish Bone-Derived Biphasic Calcium Phosphate Scaffolds Coated with Sol-Gel Derived Bioactive Glass

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2711
Author(s):  
Ana S. Neto ◽  
Daniela Brazete ◽  
José M.F. Ferreira
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Phosphates ◽  
Sol Gel ◽  
Biological Properties ◽  
X Ray Diffraction ◽  
Bioactive Glasses ◽  
Hydrothermal Transformation

The combination of calcium phosphates with bioactive glasses (BG) has received an increased interest in the field of bone tissue engineering. In the present work, biphasic calcium phosphates (BCP) obtained by hydrothermal transformation of cuttlefish bone (CB) were coated with a Sr-, Mg- and Zn-doped sol-gel derived BG. The scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The initial CB structure was maintained after hydrothermal transformation (HT) and the scaffold functionalization did not jeopardize the internal structure. The results of the in-vitro bioactivity after immersing the BG coated scaffolds in simulated body fluid (SBF) for 15 days showed the formation of apatite on the surface of the scaffolds. Overall, the functionalized CB derived BCP scaffolds revealed promising properties, but further assessment of the in-vitro biological properties is needed before being considered for their use in bone tissue engineering applications.

In Vitro Cytotoxicity Study of the Nano-Hydroxyapatite/Bacterial Cellulose Nanocomposites

2009 ◽  
Vol 610-613 ◽  
pp. 1011-1016 ◽  
Author(s):  
Yan Mei Chen ◽  
Ting Fei Xi ◽  
Yu Dong Zheng ◽  
Yi Zao Wan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Biological Response ◽  
Biological Properties ◽  
In Vitro Cytotoxicity ◽  
National Standard ◽  
Target Cells

The nanocomposite of nano-hydroxyapatite/bacterial cellulose (nHA/BC) obtained by depositing in simulated body fluid (SBF), incorporating their excellent mechanical and biological properties, is expected to have potential applications in bone tissue engineering. However, the biological response evaluation of biomaterials is required to provide useful information to improve their design and application. In this article, the in vitro cytotoxicity of composites nHA/BC as well as its degradation residues was studied. Scanning electron microscopy (SEM) was used to observe the morphology of original materials and their degradation residues. The degree of degradation was evalued by measuring the concentration of reducing sugar (glucose) by ultraviolet spectrophotometer. Bone-forming osteoblasts (OB) and infinite culture cell line L929 fibroblasts were used to measure the cytotocixity of materials with MTT assay. Both kinds of cells in infusion proliferate greatly in a normal form and their relative growth rate (RGR) exceeds by 75%, which shows the cytotoxicity of materials is graded as 0~1, according to the national standard. Nevertheless, bone-forming OB cells, as a kind of target cells, are more susceptive on the cytotoxicity than infinite culture fibroblast cells L929. The results suggest the nanocomposite of nHA/BC without cytotoxicity is greatly promising as a kind of scaffold materials for bone tissue engineering and tissue functional cells are more suited to evaluate the cytotoxicity of biomedical materials.

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