Effects of Extraction Conditions on Antioxidant Activities of Roselle (Hibiscus sabdariffa L.) Extracts

2020 ◽  
Vol 977 ◽  
pp. 201-206 ◽  
Author(s):  
Quoc Duy Nguyen ◽  
Tri Nhut Pham ◽  
Mai Le Thanh Binh ◽  
Ma Thuan ◽  
Ngo Thi Thanh Van ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Solid Ratio

The objective of this study was to determine the optimal value of factors affecting the extraction of antioxidant activity of Roselle calyces. The investigated factors included extraction temperatures (30, 40, 50, 60, 70 and 80°C), extraction time (30, 60, 90, 120 and 150 minutes) and solid to solvent ratio (1:5, 1:10, 1:20, 1:40 and 1:50 g/mL). The efficiency of extraction was evaluated based on antioxidant activities which were measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay, ferric reducing antioxidant power assay (FRAP). The results showed that when the extraction temperature range of 70–80°C, DPPH free radical scavenging and FRAP were highest with 677.47–725.81 μmolTE/L and 4186.91–4391.62 μmolTE/L, respectively. For the extraction time from 120 to 150 minutes, the resulting extracts with highest FRAP and DPPH free radical scavenging antioxidant capacity were obtained. Meanwhile, the antioxidant activity tended to decrease as the solvent/solid ratio increased.

Extraction and Determination of Antioxidant Activity of Vietnamese Butterfly Pea (Clitoria ternatia L.)

2020 ◽  
Vol 977 ◽  
pp. 207-211
Author(s):  
Quoc Duy Nguyen ◽  
Thi My Hao Nguyen ◽  
Tri Duc Lam ◽  
Tri Nhut Pham ◽  
Tran Trung Thanh
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Extraction Process ◽  
Extraction Time ◽  
Time Range ◽  
Solvent Ratio ◽  
Antioxidant Power ◽  
Factors Affecting ◽  
Butterfly Pea

The objective of this study was to determine the optimal value of factors affecting the antioxidant activity extraction of antioxidant activity of butterfly pea flowers. The investigated factors included extraction temperatures (30, 40, 50, 60, 70 and 80°C), extraction time (30, 60, 90, 120 and 150 minutes) and solid to solvent ratio (1:05, 1:10, 1:20, 1:40 and 1:50 g/mL). The efficiency of extraction was evaluated based on antioxidant activities which were measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and ferric reducing antioxidant power assay (FRAP). The results showed that when the extraction process was performed at temperatures of 60°C, the resulting extracts with highest DPPH antioxidant activity (575.10 μmolTE/L) and FRAP (1093.83 μmolTE/L) was obtained. Considering the effect of extraction time on antioxidant activities of butterfly pea, time range of 30–90 minutes led to highest DPPH values while extracts with highest FRAP power were collected after 120 minutes. Moreover, decreasing solid-to-solvent ratio resulted in the decline in antioxidant activities.

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

scholarly journals Chemopreventative Potential of Plant-Based Extracts Coffee (Coffea) and Mauby (Colubrina Arborescens) using the Global Antioxidant Response (GAR) Method via in vitro Simulated Digestion

2021 ◽  
Vol 10 (3) ◽  
pp. 43
Author(s):  
Hannah Gross ◽  
Cheryl Rock ◽  
Yada Treesukosol ◽  
Virginia Gray
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Response ◽  
Free Radical Scavenging ◽  
Antioxidant Power ◽  
Simulated Digestion ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Plant-based extracts such as coffee (coffea) and Mauby (Colubrina Arborescens) were tested for chemopreventative potential by measuring their antioxidant activity (i.e., reducing power and free radical scavenging capacity) conventionally using chemical assays 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP). Alternately, extracts were also analyzed for their chemopreventative potential via a novel method (Global Antioxidant Response [GAR]), where they were subjected to simulated digestion before their antioxidant activity was determined via conventional methods previously mentioned. Next, the antioxidant capacities of the extracts, conventional versus the novel (GAR) method were compared. Overall, the analysis indicated that the reducing power (FRAP) and free radical scavenging capacity (DPPH) of coffee and Mauby were reduced (~16%) after undergoing simulated digestion. It was also observed that while the antioxidants in Mauby scavenged radicals at a significantly higher capacity than those in coffee (95.7% ± 0.67 and 90% ± 2.1 before digestion, and 77.7% ± 2.2 and 74.6% ± 2.3 after digestion, respectively), antioxidants in coffee exhibited higher reducing power compared to those in Mauby. Specifically, after undergoing simulated digestion, 1.12 mM FeSO4/mL ± 0.05 to 0.68 mM FeSO4/mL ± 0.07 ions were reduced before digestion, and 0.73 mM FeSO4/mL ± 0.09 to 0.48 mM FeSO4/mL ± 0.04 ions were reduced after digestion for coffee and Mauby, respectively. These findings suggest that while the antioxidants in coffee may have been more powerful in their ability to reduce ions, the antioxidants in Mauby may have been more effective in scavenging and neutralizing radicals.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

2020 ◽  
Vol 18 (4) ◽  
pp. 386-391
Author(s):  
Yonghun Kim ◽  
Ang Li ◽  
Junyu Wang ◽  
Wancong Yu ◽  
Fang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Therapeutic Effects ◽  
Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

scholarly journals Antioxidant Activity and Hepatoprotective Potential ofPolyalthia longifoliaandCassia spectabilisLeaves against Paracetamol-Induced Liver Injury

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Subramanion L. Jothy ◽  
Azlan Aziz ◽  
Yeng Chen ◽  
Sreenivasan Sasidharan
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Nitric Oxide Radical

In the present study,in vitroantioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts fromPolyalthia longifoliaandCassia spectabiliswere evaluated using establishedin vitromodels such as ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl(DPPH•), hydroxyl radical(OH•), nitric oxide radical(NO•)scavenging, metal chelating, and antilipidperoxidation activities. Interestingly, all the extracts showed considerablein vitroantioxidant and free radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence of strong antioxidant compound in leaf extracts tested. Phenolic and flavonoid content of these extracts is significantly correlated with antioxidant capacity. SinceP. longifoliaextract was exhibited betterin vitroantioxidant activities, it was subjected forin vivohepatoprotective activity in paracetamol-intoxicated mice. Therapy ofP. longifoliashowed the liver protective effect on biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the maximum improvement in the histoarchitecture of the liver. Results revealed thatP. longifolialeaf extract could protect the liver against paracetamol-induced oxidative damage by possibly increasing the antioxidant protection mechanism in mice. Our findings indicated thatP. longifoliaandC. spectabilishave potential as good sources of natural antioxidant/antiaging compounds.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

Disparities of phytochemical constituents and antioxidant activities of some Indigofera species

2021 ◽  
pp. 1-10
Author(s):  
Firdose R. Kolar ◽  
Sidanand V. Kambhar ◽  
Manjula Chavan ◽  
Shruti Kadam ◽  
Peerambi Nadaf
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Material ◽  
Pearson Correlation ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phytochemical Constituents

Abstract The current study was intended to examine the difference in the phytochemical and antioxidant profile of nine Indigofera species. With this aim, the species were assessed for total phenolic content, flavonoid content, and antioxidant activity using ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, ferrous ion chelating activity, phosphomolybdenum reducing power, deoxyribose degradation, β-carotene bleaching, nitric oxide scavenging, and superoxide radical scavenging assays. The content of total phenolics and flavonoids ranged from 1.33 to 22.5 mg TAE/g plant material and 0.47 to 5.02 mg QE/g plant material respectively in the various species, while the antioxidant activity as tested with different antioxidant test models varied with the species studied as well as with the solvents used for the extraction. Interestingly, all the tested extracts demonstrated considerable free radical scavenging activity, moreover, I. hirsuta, I. glandulosa, I. linnaei, I. hochstetteri, I. linifolia, and I. trita were found to be the most effective among the species studied. The results indicated the significant differences in phytochemical constituents and the antioxidant activity among the species. The Pearson correlation coefficient analysis exhibited a significant correlation of phenolics and flavonoids with the antioxidant activity.

Changes in Polyphenols Distribution and Antioxidant Activity during Fermentation of Some Underutilized Legumes

2009 ◽  
Vol 15 (1) ◽  
pp. 41-46 ◽  
Author(s):  
G. Oboh ◽  
A.O. Ademiluyi ◽  
A.A. Akindahunsi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phenol Content ◽  
Radical Scavenging Ability

The effect of fermentation on the polyphenol distribution and antioxidant activity of four underutilized legumes [Cajanus cajan L. Millsp (Pigeon pea), Vigna subterranea L. Verdc (Bambara groundnut), Sphenostylis stenocarpa Harms (African yam bean), and Phaseolus vulgaris L. (Kidney bean)] were investigated. The beans were cooked (12 h), soaked in boiled water (12 h), dehulled, and then cooked again (2 h). The cotyledons were drained, wrapped in jute sacks and left to ferment at 37°C (4 days) to produce condiments. The distribution of free and bound phenolic compounds in the fermented and unfermented beans was determined; thereafter the free radical scavenging ability, reducing power, and the ability of the free and bound phenolic compounds of the fermented and unfermented beans to inhibit lipid peroxidation were determined. The results of the study revealed that fermentation caused a significant increase (p<0.05) in the free soluble phenol content of the legumes, while there was a significant decrease (p<0.05) in the bound phenol content of the legumes. Free soluble phenol from both the fermented and unfermented legumes had a significantly (p<0.05) higher reducing power (except C. cajan), free radical scavenging ability (except C. cajan) and inhibition of lipid peroxidation than bound phenolic extract. However, free soluble phenolic compounds from the fermented beans had a significantly higher (p<0.05) reducing power, free radical scavenging ability, and inhibition of lipid peroxidation than free soluble phenols from unfermented beans. Hence, it was concluded that, fermentation could increase the free soluble phenolic content of the underutilized legumes tested and consequently enhance the antioxidant activities of the legumes; fermented V. subterranea was found to be the most promising condiment with antioxidant activity.

Total Phenolic Content and Free Radical Scavenging Activity of Nelumbo nucifera Gaertn.

2019 ◽  
Vol 886 ◽  
pp. 52-55
Author(s):  
Khemjira Jarmkom ◽  
Nakuntwalai Wisidsri ◽  
Pattaranut Eakwaropas ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Significant Difference

All parts of N. nucifera are used as oriental medicine for various medicinal purposes. The aimed of this study was to determine phenolic compound and antioxidant activity of different parts of three lotus (N. nucifera including of Roseum Plenum (RP), Album Plenum (AP), and Hindu Lotus (HL)). Total phenolic content using Folin-Ciocalteu reagent method and free radical scavenging activities using DPPH assay were determined. Three deferent parts of lotus (leaves, stem, and flower) were determined. The result indicated that Roseum Plenum leaf showed the highest total phenolic contents and Roseum Plenum flower showed the highest antioxidant activities. Total phenolic content of different part of different of N. nucifera showed a significant difference (p < 0.05). It also was not uniform when compare between different of N. Nucifera. The results found that the total phenolic content was the opposite of antioxidant activity. This result may be attributed to antioxidant activity may not be from phenolic content. Therefore, it is important to research and develop the potential of lotus extraction in the future.

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