Hardware Design for Multiple Gas Detection System Using Zeolite Coated with Nile Red

2011 ◽  
Vol 2011 (1) ◽  
pp. 000861-000867
Author(s):  
Son Nguyen ◽  
Z. Joan Delalic ◽  
David M. Kargbo ◽  
Joel B. Sheffield ◽  
Zameer Hasan
Keyword(s):  
Fluorescent Dyes ◽  
Detection System ◽  
Nile Red ◽  
Sensor Arrays ◽  
Gas Absorption ◽  
Fully Integrated ◽  
Vlsi Chip ◽  
Dye Sensing ◽  
Different Gases ◽  
Nanoporous Structures

The goal of this research is to develop a nanosensor that integrates a zeolite/dye sensing unit with an optoelectronic detector, fully integrated into a portable gas sensor. The device will detect and measure more than one target gas at the same time. Since nanoporous structures of zeolites are manipulated, the device is expected to be more accurate, more sensitive, is able to better differentiate and detect any one target in a mixture of different gases. This is achieved by incorporating fluorescent dyes into the zeolites’ cavities, measuring gas absorption, desorption and photo-chromic interaction of dye and gases, interfacing the zeolite/dye sensor arrays with light source and electronic detectors. The electronic part of the device is fully customized VLSI chip. The final device will be packaged into a portable unit. The designed and packaged prototype will be presented.

Hardware Design for Multiple Gas Detection System Using Zeolite Coated with Nile Red

2012 ◽  
Vol 2012 (1) ◽  
pp. 001185-001190
Author(s):  
Son Nguyen ◽  
Z. Joan Delalic ◽  
David M. Kargbo ◽  
Joel B. Sheffield
Keyword(s):  
Fluorescent Dyes ◽  
Gas Sensing ◽  
Zeolite Y ◽  
Detection System ◽  
Nile Red ◽  
Sensor Arrays ◽  
Gas Absorption ◽  
Red Dye ◽  
Different Gases ◽  
Nanoporous Structures

The research goal is to develop a multiple gas sensing device which integrates a zeolite-Y/nile-red sensing front end with optoelectronic detector. The highly fluorescent nile red dye, included in the nanoporous structures of zeolite Y, responses to different gases by emitting fluorescence of different wavelengths. In addition, the size of the pores of zeolite Y's can be utilized to allow gases whose molecule is smaller than the pores to enter and react with the included dye. Since nanoporous structures of zeolites are manipulated, the device is expected to be more accurate, and more sensitive. Also it is able to better differentiate and detect one target in a mixture of different gases. This is achieved by incorporating fluorescent dyes into the supercages of zeolite Y's, measuring gas absorption, desorption and photo-chromic interaction of dye and gases, interfacing the zeolite/dye sensor arrays with light source and electronic detectors.

En face dual fluorescence staining of fatty streaks allows observation of initiation and progression of atherosclerotic lesions

1995 ◽  
Vol 53 ◽  
pp. 864-865
Author(s):  
Anne M. Klinkner ◽  
Crystal R. Waites ◽  
Peter J. Bugelski ◽  
William D. Kerns
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Dimethyl Formamide ◽  
High Cholesterol Diet ◽  
Methods Development ◽  
Atherosclerotic Lesions ◽  
En Face ◽  
Biochemical Evaluation ◽  
Stock Solutions ◽  
Dual Staining

A primary effort in the understanding of the progression of atherosclerotic disease has been methods development for visualization of the atherosclerotic plaque. We introduce a new method for the qualitative analysis of lipids in atherosclerotic fatty streaks which also retains those lipids for biochemical evaluation. An original aspect of the process is the ability to view an entire fatty streak en face, selectively stained for specific lipid classes within the lesion.New Zealand white rabbits were fed a high cholesterol diet(0.15%-0.3% for 14 wks). The aorta was removed and fixed in Carson's phosphate buffered formaldehyde followed by dual staining in the fluorescent dyes Nile red and filipin. Stock solutions of nile red(0.5mg/ml acetone) and filipin(2.5mg/ml dimethyl formamide) were prepared and kept at -20°C; all subsequent steps were at RT. 0.5cm × 1.0cm pieces of aorta were trimmed and adventitia removed. The pieces were then washed 3×15 min in PBS w/o CaMg, soaked in Nile red(NR)/filipin(Fl) stain(100(il NR stock + 200μl Fl stock in 10 ml PBS for 30 min, washed in PBS 3×30 min, rinsed with distilled water, mounted(Crystal Mount, Biomedia) and coverslipped and viewed by fluorescence microscopy.

scholarly journals Visualizing the Integrity of Chloroplast Envelope by Rhodamine and Nile Red Staining

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinjie An ◽  
Xin Miao ◽  
Lulu Wang ◽  
Xu Li ◽  
Xiaomin Liu ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Gradient Centrifugation ◽  
Nile Red ◽  
Middle Layer ◽  
Chloroplast Envelope ◽  
The Past ◽  
Biochemical Methods ◽  
Percoll Density Gradient Centrifugation ◽  
Percoll Density Gradient ◽  
Isolated Chloroplasts

Chloroplasts are essential organelles in plant cells with many important functions. Chloroplasts isolated by Percoll density gradient centrifugation are widely used in the study of chloroplasts. The intactness of isolated chloroplasts is necessary for many of the experiments. In the past, those isolated chloroplasts were either simply believed to be intact or had to be analyzed by indirect biochemical methods. Here we show a new method to check the intactness of isolated chloroplasts by staining their envelope with fluorescent dyes, Rhodamine or Nile red, and then observing them with a fluorescence microscope. With this method, broken chloroplasts and intact chloroplasts can be distinguished easily and their integrity can be checked in a few minutes. Results of this method agreed well with those of biochemical methods. Moreover, we have also found that sometimes the middle layer chloroplasts from the Percoll gradient centrifugation could be mostly broken, which could cause mistakes in the experiment. With our method, this problem can be easily found. This chloroplast envelope staining method can be used in the preparation of isolated chloroplasts to ensure the intactness.

A fully integrated bacterial pathogen detection system based on count-on-a-cartridge platform for rapid, ultrasensitive, highly accurate and culture-free assay

2020 ◽  
Vol 152 ◽  
pp. 112007 ◽  
Author(s):  
Won-Il Lee ◽  
Younghyeon Park ◽  
Sajal Shrivastava ◽  
Taekeon Jung ◽  
Montri Meeseepong ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Detection System ◽  
Bacterial Pathogen ◽  
Fully Integrated

scholarly journals A fully integrated violence detection system using CNN and LSTM

2021 ◽  
Vol 11 (4) ◽  
pp. 3374
Author(s):  
Sarthak Sharma ◽  
B. Sudharsan ◽  
Saamaja Naraharisetti ◽  
Vimarsh Trehan ◽  
Kayalvizhi Jayavel
Keyword(s):  
Real Time ◽  
Detection System ◽  
Police Department ◽  
Learning Networks ◽  
Shopping Malls ◽  
Fully Integrated ◽  
Video Footage ◽  
Real Time Analysis ◽  
Violence Detection ◽  
Sports Stadiums

Recently, the number of violence-related cases in places such as remote roads, pathways, shopping malls, elevators, sports stadiums, and liquor shops, has increased drastically which are unfortunately discovered only after it’s too late. The aim is to create a complete system that can perform real-time video analysis which will help recognize the presence of any violent activities and notify the same to the concerned authority, such as the police department of the corresponding area. Using the deep learning networks CNN and LSTM along with a well-defined system architecture, we have achieved an efficient solution that can be used for real-time analysis of video footage so that the concerned authority can monitor the situation through a mobile application that can notify about an occurrence of a violent event immediately.

scholarly journals Redox-Responsive Nanocarrier for Controlled Release of Drugs in Inflammatory Skin Diseases

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 37
Author(s):  
Keerthana Rajes ◽  
Karolina A. Walker ◽  
Sabrina Hadam ◽  
Fatemeh Zabihi ◽  
Fiorenza Rancan ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Skin Diseases ◽  
Ex Vivo ◽  
Nile Red ◽  
Redox Potentials ◽  
Inflammatory Skin Diseases ◽  
Molecular Weights ◽  
Molecular Weight Distributions ◽  
Redox Responsive ◽  
20 Nm

A synthetic route for redox-sensitive and non-sensitive core multi-shell (CMS) carriers with sizes below 20 nm and narrow molecular weight distributions was established. Cyclic voltammetric measurements were conducted characterizing the redox potentials of reduction-sensitive CMS while showcasing its reducibility through glutathione and tris(2-carboxyethyl)-phosphine as a proof of concept. Measurements of reduction-initiated release of the model dye Nile red by time-dependent fluorescence spectroscopy showed a pronounced release for the redox-sensitive CMS nanocarrier (up to 90% within 24 h) while the non-sensitive nanocarriers showed no release in PBS. Penetration experiments using ex vivo human skin showed that the redox-sensitive CMS nanocarrier could deliver higher percentages of the loaded macrocyclic dye meso-tetra (m-hydroxyphenyl) porphyrin (mTHPP) to the skin as compared to the non-sensitive CMS nanocarrier. Encapsulation experiments showed that these CMS nanocarriers can encapsulate dyes or drugs with different molecular weights and hydrophobicity. A drug content of 1 to 6 wt% was achieved for the anti-inflammatory drugs dexamethasone and rapamycin as well as fluorescent dyes such as Nile red and porphyrins. These results show that redox-initiated drug release is a promising strategy to improve the topical drug delivery of macrolide drugs.

scholarly journals An Affordable Image Detector and a Low-Cost Evaluation System for Computed Tomography Using Neutrons, X-rays or Visible Light

2019 ◽  
Vol 3 (4) ◽  
pp. 21 ◽  
Author(s):  
Schillinger
Keyword(s):  
Computed Tomography ◽  
Visible Light ◽  
Evaluation System ◽  
Detection System ◽  
Low Cost ◽  
Cost Evaluation ◽  
X Rays ◽  
Fully Integrated ◽  
Motion System ◽  
Quality Detection

Neutron computed tomography (nCT) has been established at many major neutron sources worldwide, using high-end equipment requiring major investment and development. Many older and smaller reactors would also be capable of doing nCT, but cannot afford the investment before feasibility is proven. We have developed a compact low-cost but high-quality detection system using a new cooled CMOS camera that can either be fully integrated into a sophisticated setup, or used with a rudimentary CT control and motion system to quickly evaluate feasibility of neutron CT at a given beam line facility. Exchanging the scintillation screen makes it feasible for X-rays as well, even for visible light (and transparent samples) using a matte screen. The control system uses a hack to combine motion control with existing imaging software so it can be used to test several dozen different cameras without writing specific drivers. Freeware software can do reconstruction and 3D imaging.

Involvement of annexin II in exocytosis of lamellar bodies from alveolar epithelial type II cells

1996 ◽  
Vol 270 (4) ◽  
pp. L668-L676 ◽  
Author(s):  
L. Liu ◽  
M. Wang ◽  
A. B. Fisher ◽  
U. J. Zimmerman
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Immunoblot Analysis ◽  
Annexin Ii ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Lamellar Bodies ◽  
Permeabilized Cells ◽  
Aggregation Activity

Annexins are a family of Ca(2+)- and phospholipid-binding proteins that have been implicated in exocytosis. In the present study, we investigated the participation of selected annexins in exocytosis of lamellar bodies by examining their liposome aggregation property and ability to reconstitute surfactant secretion from permeabilized rat lung alveolar type II cells. Annexins I, II, III, and VI were demonstrated in type II cells by immunoblot analysis, but annexin IV and V were not found. Annexins I-IV mediated liposome aggregation in the presence of 1 mM Ca2+. However, only annexin II tetramer had aggregation activity at 10 microM Ca2+. Annexins V and VI had negligible aggregation activity at any Ca2+ concentrations (up to 1 mM Ca2+). To study reconstitution of secretion by annexins, isolated type II cells were permeabilized with 40 microM beta-escin. Under these conditions, the permeabilized cells released approximately 30-40% lactic acid dehydrogenase into the medium. An underestimated fraction of cellular annexin content was lost during permeabilization. However, lamellar bodies in the permeabilized type II cells stained appropriately with the fluorescent dyes Nile red and quinacrine, indicating that they were intact. These permeabilized cells were secretion competent, since phosphatidylcholine (PC) secretion was stimulated by 0.2-1.0 microM Ca2+. Addition of an exogenous annexin mixture enhanced PC secretion from the permeabilized type II cells with maximal stimulation at 0.5 microM Ca2+. Of six purified annexins (I-VI) tested for their ability to reconstitute secretion from permeabilized cells, only annexin II was effective. Our results suggest that annexin II is not necessary for exocytosis of lamellar bodies.

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