scholarly journals Volume and surface changes in vero cell and its nucleus after infection with measles virus: a stereological study

2011 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
Ali Noorafshan ◽  
Mohammad Motamedifar ◽  
Saied Karbalay-Doust
Keyword(s):  
Vero Cell ◽  
Post Infection ◽  
Measles Virus ◽  
Plaque Assay ◽  
Vero Cells ◽  
Uninfected Control ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
Infected Cells ◽  
The Mean

Measles virus has no or indistinctive cytopathic effects (CPE) in cell couture system. Employment of some detecting methods like plaque assay or stereologic experiments, as a method of detecting of viral infection in the cells would be applicable. The aim of this study was investigating the early changes in quantitative parameters of measles virus infected Vero cells. Stereological methods using invariator, were applied for the first time to estimate cell and nucleus volume and cell surface of the infected Vero cell line with the measles virus.This method can be applied on other cultured cells.Vero cells grown in tissue culture plates for 48 hours at 36˚C were infected with 100TCID50 of AiK strain of measles virus. Volume and surface of the infected Vero cells were studied at 4, 9 and 25 hours post infection along with uninfected control cells. The mean cell volume and surface of the cells infected with measles virus, increased ~87% and ~50%, respectively, 4 hours post-infection, as compared with the uninfected control. The nuclei did not show any differences. The mean parameters of infected cells in other time intervals showed no significant difference comparing with the control cells. Although there are other specific methods, stereology may be used as an integrated protocol to detect cytophatic changes of the measles virus infected cells early in the permissive cell culture system.

scholarly journals Blood Donation in Nigeria: Standard of the Donated Blood

2012 ◽  
Vol 4 (02) ◽  
pp. 094-097 ◽  
Author(s):  
Nwogoh Benedict ◽  
Awodu Omolade Augustina ◽  
Bazuaye Godwin Nosakhare
Keyword(s):  
Blood Donation ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Mean Cell Volume ◽  
Benin City ◽  
Cell Counts ◽  
Significant Difference ◽  
The Mean

ABSTRACT Objective: The objective was to determine the basic hematological parameters of remunerated blood donors in Benin City and to compare them with those of voluntary donors. Materials and Methods: This is a prospective study conducted in a tertiary health facility in Benin City. Pretransfusion samples were obtained from blood bags after gentle mixing and analyzed for hematological parameters. Samples were analyzed using the hematology autoanalyzer MODEL SYSMEX KN21. Result: A total of 215 samples were obtained comprising 160 remunerated (paid) and 55 voluntary donor samples. In the paid donors, the mean hemoglobin concentration (Hb) and hematocrit (HCT) 7.7±2.9 and 28.8±8.5 respectively. This was significantly lower than those of voluntary donors who had 13.9±1.2 and 42.2±3.3 with P < 0.001. The mean values of the red cell counts (RBC), white cell counts (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in paid donors as P-values were <0.001. MCV was significantly low but not compared to the other parameters as P=0.04. There was no significant difference in the platelet count. Conclusion: Paid donors in Benin City have significantly lower hematological parameters than controls.

scholarly journals Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1657
Author(s):  
Hila Winer ◽  
Janet Ozer ◽  
Yonat Shemer ◽  
Irit Reichenstein ◽  
Brit Eilam-Frenkel ◽  
...  
Keyword(s):  
Measles Virus ◽  
Acute Infection ◽  
Vero Cells ◽  
Transcriptional Level ◽  
Virus Infectivity ◽  
Water Lily ◽  
African Green Monkey Kidney ◽  
Viral Rnas ◽  
Nuphar Lutea ◽  
Infected Cells

Different parts of Nuphar lutea L. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that Nuphar lutea extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.

scholarly journals Measles Virus Attenuation Associated with Transcriptional Impediment and a Few Amino Acid Changes in the Polymerase and Accessory Proteins

1998 ◽  
Vol 72 (11) ◽  
pp. 8690-8696 ◽  
Author(s):  
Makoto Takeda ◽  
Atsushi Kato ◽  
Fumio Kobune ◽  
Hiroko Sakata ◽  
Yan Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Vero Cell ◽  
Cell Line ◽  
Measles Virus ◽  
Vero Cells ◽  
Functional Difference ◽  
Pathogenic Potential ◽  
P Proteins

ABSTRACT Measles virus (MV) isolated in B95a cells, a marmoset B-cell line, retains full pathogenicity for cynomolgus monkeys, while its derivative obtained by adaptation to the growth in Vero cells, a monkey kidney cell line, loses the pathogenic potential (F. Kobune, H. Sakata, and A. Sugiura, J. Virol. 64:700–705, 1990). Here, we show with a pair of strains, a fresh isolate (9301B) in B95a cells and its Vero cell-adapted form (9301V), that the in vivo attenuation parallels the decrease of replication and syncytium-inducing capabilities in the original B95a cells and that these in vitro phenotypes are attributable to impediment of transcription, which is already obvious at the level of primary transcription catalyzed by the virion-associated RNA polymerase. On the other hand, cell fusion assays detected no functional difference between the glycoproteins of the two viruses. Essentially the same transcriptional impediment with reduced syncytium induction following Vero cell adaptation was found with two other pairs of strains that had been similarly prepared. Nucleotide sequence comparison between the 9301B and 9301V viruses revealed that a few (at most five) amino acid changes, which sporadically took place in the polymerase (L and P proteins) and/or accessory V and C proteins, were responsible for the in vitro and in vivo attenuation through adaptation to growth in Vero cells.

scholarly journals Measles-virus-persistent infection in BGM cells. Modification of the incorporation of [3H]arachidonic acid and [14C]stearic acid into lipids

1983 ◽  
Vol 214 (3) ◽  
pp. 665-670 ◽  
Author(s):  
P Anderton ◽  
T F Wild ◽  
G Zwingelstein
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Stearic Acid ◽  
Measles Virus ◽  
Total Phospholipid ◽  
Persistently Infected ◽  
Esterified Fatty Acids ◽  
Acid Incorporation ◽  
Significant Difference ◽  
Infected Cells

In BGM cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. Uninfected, lytic and persistently infected cells were labelled with [3H]arachidonic acid and [14C]stearic acid and their metabolic fate analysed. No difference in the total incorporation was observed in the different systems. However, the [14C]stearic acid and [3H]arachidonic acid were incorporated up to 2-fold and 13-fold respectively greater into the neutral lipid of persistently infected compared with that of uninfected cells. Both radioactive fatty acids were specifically accumulated in the triacylglycerol and non-esterified fatty acids fractions. Lytically infected cells were similar to uninfected cells. Although there was no significant difference in the incorporation of radioactivity into the total phospholipid in either system, there was a large decrease in [3H]arachidonic acid incorporated into phosphatidylethanolamine and to a lesser extent phosphatidylcholine and phosphatidylinositol in persistently infected cells. [14C]Stearic acid incorporation was also reduced in phosphatidylcholine and phosphatidylethanolamine fractions of persistently infected cells.

scholarly journals Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Kleber Juvenal Silva Farias ◽  
Paula Renata Lima Machado ◽  
Benedito Antônio Lopes da Fonseca
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Plaque Assay ◽  
Antiviral Drug ◽  
Vero Cells ◽  
Significant Difference ◽  
Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

scholarly journals Some hematological parameters of Wistar rats treated with Chromolaena odorata leave extracts

2017 ◽  
Vol 90 (1) ◽  
Author(s):  
Henshaw Uchechi Okoroiwu ◽  
Ifeyinwa Maryann Okafor ◽  
Emmanuel Kufre Uko ◽  
Item Justin Atangwho
Keyword(s):  
Cell Volume ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Control Group ◽  
Chromolaena Odorata ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
The Mean ◽  
Ethanol Extracts

This study was designed to investigate the effects of the different extracts of Chromolaena odorata leave on the hematopoietic system of Wistar rats. Solvent extraction was used for the ethanol and aqueous extractions while decoction method was used for the crude extraction. Fifty Wistar rats of both sexes weighing 140-180 g were used for this study. They were divided into ten groups each containing five rats. The animals were fed the extracts by oral gavage once daily for 21 days. Blood sample was collected via cardiac artery. Hematological parameters were analyzed using automation method. The ethanol extract gave the highest extract yield. The aqueous, ethanol and crude extraction had median lethal toxicity (LD50) of 2738.6 mg/kg, 1581.1 mg/kg and 224.7 mg/kg, respectively. Significant difference (P<0.05) in the total white blood cell count was observed in the 75 mg/kg ethanol and 300 mg/kg crude extracts when compared with control group. Significant difference (P<0.05) in the hemoglobin concentration was observed in the 150 mg/kg ethanol extracts when compared with the control group. Significant difference (P<0.05) in the packed cell volume was seen in the 75 mg/kg aqueous, 150 mg/kg aqueous and 75 mg/kg ethanol extracts in respect to the control group. The mean cell volume, the mean platelet volume and platelet large cell ratio of the 75 mg/kg aqueous extract were significantly different (P<0.05) when compared with the control group. The present study showed possible treatment-induced hematopoietic function of C. odorata leave extracts.

The influence of anaesthetics on the haematology of the patas monkey, Erythrocebus patas

1978 ◽  
Vol 12 (3) ◽  
pp. 167-170 ◽  
Author(s):  
C. M. Hawkey ◽  
S. Dean ◽  
M. G. Hart
Keyword(s):  
Cell Count ◽  
Cell Volume ◽  
Fibrinolytic Activity ◽  
White Cell Count ◽  
Erythrocebus Patas ◽  
Suitable Alternative ◽  
Mean Cell Volume ◽  
Patas Monkey ◽  
Significant Difference ◽  
The Mean

The effects of phencyclidine, ketamine and an alphaxalone-alphadolone mixture on the haematology of the patas monkey have been compared. In animals sedated with phencyclidine or ketamine the only significant difference detected was in the mean cell volume. Statistically significant differences in white-cell count and blood coagulation and fibrinolytic activity were found in monkeys which had received alphaxalone-alphadolone. It is suggested that ketamine is a suitable alternative to phencyclidine for haematological studies in these monkeys.

Availability of caecal habitat as a density-dependent limit on survivorship ofLeptorhynchoides thecatusin green sunfish,Lepomis cyanellus

Parasitology ◽  
1989 ◽  
Vol 98 (3) ◽  
pp. 447-450 ◽  
Author(s):  
Julie A. Ewald ◽  
B. B. Nickol
Keyword(s):  
Post Infection ◽  
Alimentary Tract ◽  
The Other ◽  
Anterior Portion ◽  
Lepomis Cyanellus ◽  
Significant Difference ◽  
The Mean ◽  
Green Sunfish ◽  
The Relationship ◽  
Leptorhynchoides Thecatus

SUMMARYDistribution ofLeptorhynchoides thecatus(Acanthocephala: Rhadinorhynchidae) among the pyloric caeca and the relationship between site and rate of maturation were studied in laboratory infectons of 10, 25 and 40 cystacanths fed to green sunfish,Lepomis cyanellus. After 1 week fish fed at each intensity had significantly different numbers of worms. By the 3rd week post-infection, parasites disappeared from the anterior portion of the intestine. At this time the mean numbers of worms recovered from 25 and 40-cystacanth infections were not significantly different. At the end of the 1st week, the area where caeca join the alimentary tract (between caecal area) and caeca numbered 6 and 7 contained significantly more worms than the other sites. By the 3rd week post-infection only caecum 7 contained significantly more worms, and at 5 weeks there was no significant difference between the number of worms present in any caecum or the between caecal area. Initially worms in the more intense infections matured more slowly, but by the 3rd week post-infection there was no significant difference in the states of maturity. The rate of maturation was not related to the site occupied.

Cell sonicates used in the analysis of how measles and herpes simplex type 1 virus infections influence Vero cell mitochondrial calcium uptake

1985 ◽  
Vol 63 (11) ◽  
pp. 1194-1197 ◽  
Author(s):  
Karen Lund ◽  
Barry Ziola
Keyword(s):  
Herpes Simplex ◽  
Vero Cell ◽  
Measles Virus ◽  
Vero Cells ◽  
Lytic Cycle ◽  
Herpes Simplex Type ◽  
Uptake System ◽  
Virus Infections ◽  
Mitochondrial Calcium Uptake

Brief sonication was used to rapidly prepare an in situ mitochondrial Ca2+ uptake system from Vero cells. This method yielded intact, functional mitochondria capable of accumulating and retaining approximately 50% of Ca2+ available in the reaction mix. Mitochondria in cells infected with herpes simplex type 1 virus exhibited a gradual decline in the initial Ca2+ uptake rate, dropping to 65% of the control rate at the end of the 12-h lytic cycle. Influence of a lytic measles virus infection on the initial Ca2+ upake rate was different. The rate initially dropped to 75% of the control rate by 9 h postinfection, then recovered to slightly above the control rate at 21 h postinfection, and finally declined to 75% of the control rate at 30 h postinfection. Neither viral infection altered the total amount of Ca2+ accumulated by Vero cell mitochondria.

Study of transcription in measles virus-infected Vero cells using cDNA probes prepared from poly(A)RNA from uninfected and infected cells

Apmis ◽  
1991 ◽  
Vol 99 (1-6) ◽  
pp. 33-41 ◽  
Author(s):  
KARL-H. KALLAND ◽  
MAY BRITT KALVENES ◽  
ANNE MARGRETE ØYAN ◽  
GUNNAR HAUKENES
Keyword(s):  
Measles Virus ◽  
Vero Cells ◽  
Infected Cells
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