scholarly journals In vitro lethal effect of Zingiber officinale R. on protoscolices of hydatid cyst from sheep liver

2011 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Mohammad Moazeni ◽  
Ali Nazer
Keyword(s):  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Cystic Echinococcosis ◽  
Methanolic Extract ◽  
Zingiber Officinale ◽  
Lethal Effect ◽  
Ginger Extract ◽  
Scolicidal Agent

Cystic echinococcosis (Hydatid disease) is a zoonotic disease caused by <em>Echinococcus granulosus</em>. The aim of this study, was to evaluate the scolicidal activity of methanolic extract of <em>Zingiber officinale</em> (Rosc.) family Zingibe - raceae, against protoscolices of hydatid cyst. Protoscolices were collected aseptically from sheep livers containing hydatid cyst and were exposed to different concentrations of ginger extract for various exposure times. Scolicidal activity of <em>Z. officinale</em> extract at concentration of 25 mg/mL was 25.6%, 39.1%, 56.7%, 83.7%, 98.1% and 100% after 10, 20, 30, 40, 50, and 60 min of exposure respectively. Scolicidal effect of this extract at concentration of 50 mg/mL was 52%, 85.8 %, 99.6% and 100% after 10, 20, 30 and 40 min of exposure respectively. <em>Z. officinale</em> extract at concentration of 100 mg/mL killed 76.5 %, 87% and 100 % of protoscolices after 10, 20 and 30 min respectivly. The results of this study showed that the methanolic extract of <em>Z. officinale</em> has high scolicidal activity and might be used as a natural scolicidal agent.

Characterization of excretory–secretory products from protoscoleces of Echinococcus granulosus and evaluation of their potential for immunodiagnosis of human cystic echinococcosis

Parasitology ◽  
2004 ◽  
Vol 129 (3) ◽  
pp. 371-378 ◽  
Author(s):  
D. CARMENA ◽  
J. MARTÍNEZ ◽  
A. BENITO ◽  
J. A. GUISANTES
Keyword(s):  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Secretory Protein ◽  
Major Protein ◽  
Healthy Donors ◽  
Secretory Products ◽  
Protein Components ◽  
Human Cystic Echinococcosis

This study describes, for the first time, the characterization of excretory–secretory antigens (ES-Ag) from Echinococcus granulosus protoscoleces, evaluating their usefulness in the immunodiagnosis of human cystic echinococcosis. ES-Ag were obtained from the first 50 h maintenance of protoscoleces in vitro. This preparation contained over 20 major protein components which could be distinguished by 1-dimensional SDS–PAGE with apparent masses between 9 and 300 kDa. The culture of of protoscoleces from liver produced a greater variety of excretory–secretory protein components than those from lung. Determination of enzymatic activities of secreted proteins revealed the presence of phosphatases, lipases and glucosidases, but no proteases. These findings were compared to those obtained from somatic extracts of protoscoleces and hydatid cyst fluid products. Immunochemical characterization was performed by immunoblotting with sera from individuals infected by cystic echinococcosis (n=15), non-hydatidic parasitoses (n=19), various liver diseases (n=24), lung neoplasia (n=16), and healthy donors (n=18). Antigens with apparent masses of 89, 74, 47/50, 32, and 20 kDa showed specificity for immunodiagnosis of human hydatidosis. The 89 and 74 kDa components corresponded to antigens not yet described in E. granulosus, whereas proteins of 41–43 kDa and 91–95 kDa were recognized by the majority of the non-hydatid sera studied.

scholarly journals Influence of Zingiber officinale Extract on Push-Out Bond Strength of Glass-Fiber Post

2018 ◽  
Vol 29 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Tânia Mara da Silva ◽  
Virgílio Vilas Boas Fernandes Junior ◽  
Rosemary Soares de Santana ◽  
Renata Marques de Melo Marinho ◽  
Márcia Carneiro Valera ◽  
...  
Keyword(s):  
Bond Strength ◽  
Glass Fiber ◽  
Zingiber Officinale ◽  
Resin Cement ◽  
Fiber Post ◽  
Ginger Extract ◽  
Etch And Rinse ◽  
Glass Fiber Post ◽  
Push Out

Abstract The aim of this study was to evaluate in vitro the influence of 20% glycolic ginger extract on the bond strength of glass-fiber post cemented with etch-and-rinse or self-etching resin cement. Forty-eight bovine roots were standardized (17±0.5 mm) and randomly divided into two groups, according to irrigant used during biomechanical preparation: NaOCl: 1% sodium hypochlorite and GEN: 20% glycolic ginger extract. Root canal was prepared and randomly assigned to one of two subgroups (n=12), according to luting protocol: self-etching (RelyX U200) and etch-and-rinse (RelyX ARC). After 48 h, the roots were sectioned perpendicularly (4 in cervical third and 3 in middle third) and submitted to push-out bond strength test (50 kgf load cell, 1.0 mm/min). Failure mode was analyzed in SEM and stereomicroscopy. Data were analyzed by three-way ANOVA and Tukey tests (p<0.05). ANOVA revealed that there were no significant differences between U200 (2.01±0.17)B and ARC (1.93±0.12)B in GEN group, and at the cervical third for both irrigants, 2.69±0.16A and 2.54±0.17A, respectively. Middle third showed lower bond strength than cervical third, regardless the irrigant. Overall, the cervical third regardless the irrigant employed and the association between NaOCl and ARC, presented better adhesive performance.

scholarly journals In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid ofEchinococcus granulosus

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Razika Zeghir-Bouteldja ◽  
Manel Amri ◽  
Saliha Aitaissa ◽  
Samia Bouaziz ◽  
Dalila Mezioug ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
In Vitro Study ◽  
No Production ◽  
In Vitro Effects ◽  
Nitric Oxide Metabolites

Hydatidosis is characterized by the long-term coexistence of larvaEchinococcus granulosusand its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity ofNO2−andONOO−24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated thatONOO−is more cytotoxic thanNO2−. In contrast,NO3−has no effect. These results suggest possible involvement ofNO2−andONOO−in antihydatic action and point the efficacy of these metabolites as scolicidal agents.

scholarly journals Abbas Dawwas Matter Al-Maliki Investigation of Biochemical Effect of Phenols Extract Isolated from Coriandrum sativum Seeds Against Echinococcus granulosus Parasite in Vitro

2008 ◽  
pp. 2-9
Keyword(s):  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Clinical Studies ◽  
Coriandrum Sativum ◽  
Biochemical Effect ◽  
Extract Concentration ◽  
The Third ◽  
Viability Percentage

This study was carried out to investigate effect of phenols extract isolated from Coriandrum sativum seeds extract against Echinococcus granulosus parasite gotten from livers of sheep infected by hydatid disease in Basrah abattoir . It was found that increase of concentration of extract led to decrease of protoscolices viability and the number of dead protoscolices increased with increase of days . Also the concentration of (0.75 gm/ml) had the higher effect on viability of protoscolices where it was noticed that this concentration killed all protoscolices after three days of treatment wheras the concentration of (0.25 gm/ml) was the less effect where it led to kill protoscolices after seven days of treatment with extract . The viability percentage reached to zero at concentration of (0.75 gm/ml) in the third day but at concentrations (0.5 and 0.6 gm/ml) was zero in the fourth day . Also it was found that protoscolices viability percentage was zero in the seventh day of treatment by using concentration of (0.25 gm/ml) . Therefore phenols extract concentration of (0.75 gm/ml) can be used as a medicinal herbal substitute instead of antibiotics but it needs further clinical studies .

Rare presentation of isolated hydatid disease of the breast

2021 ◽  
Vol 14 (7) ◽  
pp. e243052
Author(s):  
Ronal Kori ◽  
Sudhir Kumar Jain ◽  
Rehan Nabi Khan
Keyword(s):  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Similar Case ◽  
Rare Condition ◽  
Rare Presentation ◽  
Diagnostic Dilemma ◽  
Endemic Areas ◽  
Accidental Host ◽  
Cattle And Sheep

Isolated hydatid disease of the breast is a rare condition, possessing a diagnostic dilemma for the clinicians. Hydatid disease is common in endemic areas affecting most commonly the liver and lungs. Other organs rarely involved are the kidney, bone and brain. It is caused by the parasite Echinococcus granulosus, widely spread by cattle and sheep. Humans are the accidental host for this organism. We present a similar case of isolated hydatid cyst of the breast, which was diagnosed preoperatively and managed successfully.

scholarly journals In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus

2013 ◽  
Vol 6 (8) ◽  
pp. 595-601 ◽  
Author(s):  
Mayank Gangwar ◽  
Vijay C Verma ◽  
Tryambak D Singh ◽  
Sushil K Singh ◽  
RK Goel ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Glandular Hair ◽  
Hair Extract

scholarly journals Unusual presentation of a Hydatid cyst: a case report

2015 ◽  
Vol 22 (2) ◽  
pp. 216-218 ◽  
Author(s):  
Asifa Sattar ◽  
Nazmun Nahar ◽  
Md Mizanur Rahman ◽  
ASM Tanim Anwar ◽  
Anwar Hossain
Keyword(s):  
Case Report ◽  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
The Body ◽  
Unusual Presentation ◽  
Parasitic Disease ◽  
College Hospital ◽  
Medical College ◽  
The World

Hydatid disease is a parasitic disease, which is most commonly caused by Echinococcus granulosus. It is endemic in many parts of the world. However, Hydatid disease can occur in almost any part of the body. Isolated omenal hydatid cyst is one of the least common sites. A case of very unusual omental hydatid cyst is presented here which was diagnosed in the Department of Radiology & Imaging, Dhaka Medical College Hospital, Dhaka, and subsequently confirmed by histopathology. DOI: http://dx.doi.org/10.3329/jdmc.v22i2.21546 J Dhaka Medical College, Vol. 22, No.2, October, 2013, Page 216-218

scholarly journals Kunitz type protease inhibitor EgKI-1 from the canine tapeworm Echinococcus granulosus as a promising anti-cancer therapeutic

2018 ◽  
Author(s):  
Shiwanthi L Ranasinghe ◽  
Glen M Boyle ◽  
Katja Fischer ◽  
Jeremy Potriquet ◽  
Jason P Mulvenna ◽  
...  
Keyword(s):  
Cell Growth ◽  
Protease Inhibitor ◽  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Cancer Cell ◽  
Dependent Manner ◽  
Anti Cancer ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor

AbstractEgKI-1, a member of the Kunitz type protease inhibitor family, is highly expressed by the oncosphere of the canine tapeworm Echinococcus granulosus, the stage that is infectious to humans and ungulates, giving rise to a hydatid cyst localized to the liver and other organs. Larval protoscoleces, which develop within the hydatid cyst, have been shown to possess anti-cancer properties, although the precise molecules involved have not been identified. We show that recombinant EgKI-1 inhibits the growth and migration of a range of human cancers including breast, melanoma and cervical cancer cell lines in a dose-dependent manner in vitro without affecting normal cell growth. Furthermore, EgKI-1 treatment arrested the cancer cell growth by disrupting the cell cycle and induced apoptosis of cancer cells in vitro. An in vivo model of triple negative breast cancer (MDA-MB-231) in BALB/c nude mice showed significant tumor growth reduction in EgKI-1-treated mice compared with controls. These findings indicate that EgKI-1 shows promise for future development as an anti-cancer therapeutic.

scholarly journals Investigation of ginger (Zingiber officinale) aqueous extract as an anti-diabetic in vitro

2021 ◽  
Vol 913 (1) ◽  
pp. 012108
Author(s):  
P Pakan ◽  
K Lidia ◽  
M Riwu
Keyword(s):  
Side Effects ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Zingiber Officinale ◽  
Control Group ◽  
Plain Water ◽  
Ginger Extract ◽  
Mouse Tissues ◽  
Treatment Of Diabetes

Abstract Diabetes mellitus is a condition of metabolic imbalance, indicated by a high level of blood glucose (hyperglycemia) resulting from a reduction of insulin secretion, action, or both. People with diabetes suffer from a lack or deficiency of insulin or insulin resistance. The metabolic imbalances are often not satisfactorily corrected using conventional medicines and even cause some side effects, which can be detrimental. Research on herbal medicines for the treatment of diabetes is urged by the need to reduce unwanted side effects common with conventional medicines/treatments used in glucose regulation. This study aims to investigate the antidiabetic effect of Ginger (Zingiber officinale) aqueous extract in improving the glucose uptake in mouse tissues in vitro. This study is a true experimental research design with a posttest-only control group design. There were three groups of mice in this study: the control group, which were only given plain water; the second group of mice with 5% aqueous ginger extract and the last group were given 25% aqueous ginger extract. All groups were given treatment for four consecutive weeks, then dissected their cardiac muscle, skeletal muscle, pancreas, and liver tissues to analyze the glucose uptake. The result showed that both the ginger aqueous extract groups were able to increase the glucose uptake of the mice. In conclusion, this research has shown that aqueous ginger extract may have improved the glucose uptake in most tissues of the mice in the groups. Therefore, ginger could have great potential as an alternative way in the treatment of diabetes type 2.

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