EFFECTS OF LYSINE INFUSION PER ABOMASUM OF STEERS FED CONTINUOUSLY

1972 ◽  
Vol 52 (4) ◽  
pp. 681-687 ◽  
Author(s):  
R. J. BOILA ◽  
T. J. DEVLIN
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Linear Increase ◽  
The Body ◽  
The Other ◽  
Body Tissue ◽  
Rumen Microorganisms

Four dairy steers were allotted to four lysine infusion levels in a 4 × 4 latin square design and fed an 11.5% crude protein (90% dry matter (DM)) diet continuously (10-min intervals every 24 hr). Lysine hydrochloride equivalent to 0.0, 3.0, 6.0, and 9.0 g lysine per day was infused per abomasum. When 9 g lysine were infused per day, the percent of absorbed nitrogen (N) retained was significantly (P < 0.05) reduced; urinary N excretion as a percentage of N intake and plasma-free lysine were increased significantly compared with the other three infusion treatments. The infusion of 9 g lysine per day apparently exceeded the body tissue requirements for this amino acid and the excess N was excreted in the urine. A possibility of lysine being limiting (0.28% lysine of a 100% DM diet) was apparently offset by the synthesis of lysine by rumen microorganisms, which increased the dietary lysine two- to threefold. Increased levels of infused lysine did not result in a linear increase of lysine in the abomasum. With 3 g per day lysine infusion rumen ammonia and N retentions were high. However, a smaller amount of N reached the abomasum with steers on this treatment.

scholarly journals Influence of Glutamine and Glutamate Supplementation in the Blood Levels of Horses

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Helena Emília Cavalcanti da Costa Cordeiro Manso ◽  
Deyvson Ferreira De Oliveira ◽  
Monica Miranda Hunka ◽  
Hélio Cordeiro Manso Filho
Keyword(s):  
Amino Acid ◽  
Latin Square ◽  
Dietary Supplementation ◽  
The Body ◽  
The Other ◽  
Glutamine Metabolism ◽  
Free Form ◽  
Control Group ◽  
Blood Levels ◽  
Total Plasma Protein

Background: The most abundant free amino acid in mammals is glutamine (GLN). Little research has focused on GLN supplementation for horses, but GLN levels in this species are known to decline after exercise and during lactation. Under physiological conditions, the body produces Gln in sufficient quantities for general metabolism, and a small part of this amino acid comes from dietary protein. Little research has so far focused on equine dietary supplementation with free glutamine or combined with other amino acids during catabolic states or in highly stressful situations. This research was conducted to evaluate the effects of equine dietary supplementation using a combination of glutamine and glutamate.  Materials, Methods & Results: The study involved four Arabian mares, not in training (~380 kg; ~12 years old) and four treatments (control, and inclusions of 1, 2 and 4% of GLN+GLU) in a Latin square model. A 7-day washout period was established between each phase. Fifty percent of the mares’ maintenance energy requirements came from concentrate and 50% from hay and grazing. The other 50% came from Tifton hay (Cynodon dactylon), which was supplied ad libitum. After 7 weeks of nutritional supplementation (once a day, in the morning). In the experimental model, the mares were distributed in a Latin square design comprised of four treatments: control (without inclusion) and inclusions of 1%, 2% and 4% of supplement (AminoGut®, Ajinomoto do Brazil), and four animals. Blood was collected in five stages (fasting, and 60, 120, 240 and 360 min after feeding) in each treatment. The blood samples were analyzed to determine GLN, GLU, urea, creatinine, uric acid, total plasma protein, hematocrit and glucose levels. Glutamine and Glutamate concentrations were analyzed using the enzymatic spectrophotometric method. The results were analyzed statistically using one- and two-way ANOVA and Tukey’s test with P set at 5%. The results indicated that GLN differed in both the group (P < 0.001) and between the phases of supplementation (P < 0.001), but no interaction occurred between them (P > 0.05). Significant changes in GLN levels were also observed in the 4% inclusion treatment compared to all the treatments in the fasting phase and in the + 60 min and +240 min phases of the control group (P < 0.05). All the other biomarkers analyzed here were unchanged (Glutamate, Urea, Creatinine, Urea, TPP, Glucose and Hematocrit) (P > 0.05) during the period under analysis, and remained within the normal range for the species in their current stabling conditions. The mares presented no clinical problems nor did they change their feeding behavior during the supplementation period or on the days blood was collected.Discussion: Glutamine metabolism in horses has yet to be extensively studied.  However, it has been shown that, when supplied to horses in its free form, this amino acid causes Gln levels to rise rapidly within the first 90 min of the postprandial period.  This indicates that an extra amount of this amino acid may increase Gln blood levels despite intense degradation of enterocytes. This study found that supplementation with a combination of Gln+Glu can increase blood Gln levels after 6 h in the treatment involving 4% Gln+Glu included in the concentrate (P < 0.05) In conclusion, supplementation with GLN+GLU raised the mares’ GLN levels after 360 min when 4% of GLN+GLU was included in their diet. These results may be used to establish GLN supplementation models for horses. 

scholarly journals Nutrients intake and digestibility in sheep fed with residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4401 ◽  
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes Costa ◽  
Jocilaine Garcia ◽  
Anderson Moura Zanine ◽  
Edmiar Barbosa de Oliveira ◽  
...  
Keyword(s):  
Body Weight ◽  
Nutrient Intake ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Linear Increase ◽  
Neutral Detergent Fiber ◽  
Regression Equations ◽  
Ether Extract ◽  
Total Carbohydrates

<p>This study aimed to evaluate nutrient intake and digestibility by sheep fed diets containing residue from the extraction of tamarind pulp (RETP). We used four sheep with a mean body weight (BW) of 40.38 kg, whose pens were distributed in a Latin square design. The diet treatments contained 0, 5, 10 and 15% of RETP. The variables were subjected to analysis of variance and the differences observed were tested using regression equations at 5% significance. The intake of dry matter (DM), organic matter (OM), crude protein (CP), total carbohydrates (TC), and non-fiber carbohydrates (NFC),expressed in g day-1, % BW and g kg0,75-1, were not significantly affected (p&gt;0.05) by the inclusion of RETP. The intake of ether extract (EE) and neutral detergent fiber (NDF) showed a linear increase (p&lt;0.05) as RETP content rose. However, the digestibility of DM, CP, TC, and total digestible nutrients (TDN) decreased linearly (p&lt;0.05), while the digestibility of EE, NDF, ADF, and NFC, with average values of 62.12%; 79.95%; 41.59%; 37.12%, and 91.57%, respectively, were not significantly affected (p&gt;0.05) when RETP was included in the diet. The inclusion of up to 15% of the residue from the extraction of tamarind pulp changes the intake of ether extract and neutral detergent fiber, and reduced dry matter, crude protein, and total carbohydrates in the sheep’s diet.</p>

Heat-treated whole cottonseed: effect of dietary protein concentration on the performance and amino acid utilization by the mammary gland of dairy cows

1999 ◽  
Vol 66 (1) ◽  
pp. 9-22 ◽  
Author(s):  
SAMEER J. MABJEESH ◽  
ISRAEL BRUCKENTAL ◽  
AMECHAI ARIELI
Keyword(s):  
Mammary Gland ◽  
Amino Acid ◽  
Milk Fat ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Amino Acid Utilization ◽  
Passive Response ◽  
Whole Cottonseed ◽  
Rumen Undegradable Protein

The effects of replacing whole cottonseed by heated whole cottonseed at two levels of crude protein on amino acid (AA) utilization by the bovine mammary gland were investigated. Four Israeli Holstein cows were used in a 4×4 Latin square design with 2×2 factorial arrangements. Diets were formulated to contain two levels of crude protein (CP), normal (160 g CP/kg dry matter) and low (140 g CP/kg dry matter), and two levels of rumen undegradable protein (UDP), high (380 g UDP/kg CP) and low (350 g UDP/kg CP). Whole cottonseed was quantitatively substituted by heated whole cottonseed to formulate the high UDP diets. Intakes of dry matter and organic matter were similar for all treatments and averaged 15·9 and 14·4 kg/d respectively. Yields of milk and its constituents were similar for all treatments. Milk yield averaged 23·1 kg/d and this contained (per kg) 32·3 g CP, 25·4 g total casein and 47·5 g lactose. The milk fat content was lower in the cows given the high UDP diets, averaging 34·6 g/kg compared with 38·9 g/kg in the cows fed on the diets with low UDP. Plasma AA concentrations were similar for all treatments apart from Leu and Ile, which were higher in the cows given the high CP diets: 152 and 103 μm compared with 183·8 and 131·5 μm for the low CP diets. Net extraction and balance of essential AA across the half udder suggested that essential AA were supplied in amounts sufficient to meet milk protein requirements for all treatments. The surplus AA supplied as a result of feeding the high dietary CP were probably catabolized via an enhanced oxidative pathway, possibly in the liver, presumably as a passive response to their disposal.

scholarly journals Nutrients intake and digestibility in sheep fed with residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4401
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes Costa ◽  
Jocilaine Garcia ◽  
Anderson Moura Zanine ◽  
Edmiar Barbosa de Oliveira ◽  
...  
Keyword(s):  
Body Weight ◽  
Nutrient Intake ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Linear Increase ◽  
Neutral Detergent Fiber ◽  
Regression Equations ◽  
Ether Extract ◽  
Total Carbohydrates

This study aimed to evaluate nutrient intake and digestibility by sheep fed diets containing residue from the extraction of tamarind pulp (RETP). We used four sheep with a mean body weight (BW) of 40.38 kg, whose pens were distributed in a Latin square design. The diet treatments contained 0, 5, 10 and 15% of RETP. The variables were subjected to analysis of variance and the differences observed were tested using regression equations at 5% significance. The intake of dry matter (DM), organic matter (OM), crude protein (CP), total carbohydrates (TC), and non-fiber carbohydrates (NFC),expressed in g day-1, % BW and g kg0,75-1, were not significantly affected (p>0.05) by the inclusion of RETP. The intake of ether extract (EE) and neutral detergent fiber (NDF) showed a linear increase (p<0.05) as RETP content rose. However, the digestibility of DM, CP, TC, and total digestible nutrients (TDN) decreased linearly (p<0.05), while the digestibility of EE, NDF, ADF, and NFC, with average values of 62.12%; 79.95%; 41.59%; 37.12%, and 91.57%, respectively, were not significantly affected (p>0.05) when RETP was included in the diet. The inclusion of up to 15% of the residue from the extraction of tamarind pulp changes the intake of ether extract and neutral detergent fiber, and reduced dry matter, crude protein, and total carbohydrates in the sheep’s diet.

scholarly journals Nutrients intake and digestibility in sheep fed with residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Body Weight ◽  
Nutrient Intake ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Linear Increase ◽  
Neutral Detergent Fiber ◽  
Regression Equations ◽  
Ether Extract ◽  
Total Carbohydrates

This study aimed to evaluate nutrient intake and digestibility by sheep fed diets containing residue from the extraction of tamarind pulp (RETP). We used four sheep with a mean body weight (BW) of 40.38 kg, whose pens were distributed in a Latin square design. The diet treatments contained 0, 5, 10 and 15% of RETP. The variables were subjected to analysis of variance and the differences observed were tested using regression equations at 5% significance. The intake of dry matter (DM), organic matter (OM), crude protein (CP), total carbohydrates (TC), and non-fiber carbohydrates (NFC),expressed in g day-1, % BW and g kg0,75-1, were not significantly affected (p>0.05) by the inclusion of RETP. The intake of ether extract (EE) and neutral detergent fiber (NDF) showed a linear increase (p<0.05) as RETP content rose. However, the digestibility of DM, CP, TC, and total digestible nutrients (TDN) decreased linearly (p<0.05), while the digestibility of EE, NDF, ADF, and NFC, with average values of 62.12%; 79.95%; 41.59%; 37.12%, and 91.57%, respectively, were not significantly affected (p>0.05) when RETP was included in the diet. The inclusion of up to 15% of the residue from the extraction of tamarind pulp changes the intake of ether extract and neutral detergent fiber, and reduced dry matter, crude protein, and total carbohydrates in the sheep’s diet.

Amino acid content and digestibile energy value of sprouted, rust-affected and sound wheat in pig diets

1975 ◽  
Vol 26 (6) ◽  
pp. 1109 ◽  
Author(s):  
MR Taverner ◽  
CJ Rayner ◽  
RS Biden
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Amino Acid Content ◽  
Light Weight ◽  
Lysine Content ◽  
Wheat Proteins ◽  
Digestible Energy

Sound wheat (FAQ) and wheat damaged by weather (light weight sprouted) and by rust infection (No. 2 and No. 3 off-grade) were analysed for proximate composition and amino acid content. The digestibilities and digestible energy contents of the wheats were determined in studies with eight pigs over four collection periods in two 4 x 4 Latin square designs. Test weights, crude protein contents, glutamic acid contents, digestibilities of dry matter, nitrogen and energy of the damaged wheats were less than those of the sound wheat, but the lysine content of damaged wheat proteins was greater than that in the protein of sound wheat. The digestible energy contents of sound, sprouted and moderately and severely rust-affected wheats were 3982, 3863, 3771 and 3633 kcal kg-1 dry matter, respectively.

scholarly journals Which non-fibrous carbohydrates: neutral detergent fiber ratio optimizes the use of cactus cladodes in diets of growing heifers?

2021 ◽  
Vol 42 (6supl2) ◽  
pp. 3947-3960
Author(s):  
Airon Aparecido Silva de Melo ◽  
◽  
Dulciene Karla de Andrade Silva ◽  
André Luiz Rodrigues Magalhães ◽  
Fábia Simone Bezerra Cordeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Ingestive Behavior ◽  
The Other ◽  
Microbial Protein ◽  
Urinary Volume ◽  
Quadratic Effect ◽  
Ruminal Ph

This study aimed to evaluate the effect of different non-fibrous carbohydrate (NFC):neutral detergent fiber (NDF) ratios (0.70:1, 0.90:1, 1.14:1, 1.42:1, and 1.85:1) in diets containing cactus cladodes on intake, digestibility, ruminal parameters, ingestive behavior, and microbial protein of growing heifers. Five rumen-cannulated heifers (194 ± 70 kg of body weight) were distributed in a Latin square design (5 x 5). There was a quadratic effect on the intake of dry matter (DM) (Kg day-1 and %BW) and crude protein (CP) with the increase in non-fibrous carbohydrate (NFC): neutral detergent fiber (NDF) ratio, with maximum values of 7.07 kg day-1, 2.86%, and 0.949 kg day-1, estimated for 1.37:1, 1.39:1, and 1.27:1 ratios. When the intake of NDF decreased, in turn, the intakes of NFC and TDN increased. The NDF digestibility decreased with increasing NFC levels in diets. The other nutrients were not influenced. The ruminal pH showed the lowest values at 4 hours after feeding, mainly for animals fed the 1.85:1 ratio. There was a quadratic effect for rumination time with maximum values estimated for an NFC:NDF ratio of 0.88:1. The time spent idle increased linearly, while total chewing time and feeding efficiency of NDF decreased. The urinary volume increased linearly. However, microbial protein synthesis and the efficiency of the synthesis were not influenced. The number of protozoa increased by 64.5% with the elevation of the NFC:NDF ratio. We recommend a NFC:NDF ratio of 1.37:1 in diets containing cactus cladodes, to improve the use of nutrients, without negatively affecting ruminal parameters.

scholarly journals Levels of neutral detergent fiber in diets with forage palm for dairy goats

2018 ◽  
Vol 70 (5) ◽  
pp. 1595-1604 ◽  
Author(s):  
N.M. Sousa ◽  
J.S. Oliveira ◽  
D.S. Silva ◽  
E.M. Santos ◽  
A.N. Medeiros ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Milk Protein ◽  
Latin Square ◽  
Linear Increase ◽  
Neutral Detergent Fiber ◽  
Dairy Goats ◽  
Dry Extract ◽  
Effective Source ◽  
Extract Protein

ABSTRACT This study aimed to evaluate the amount of fiber in the diet that is able to promote better use of nutrients in the diet of dairy goats when forage palm is the main source of roughage. Fifteen Alpine dairy goats were distributed in a 5 × 5 triple Latin square containing five treatments and five periods, and each treatment contained. We tested five diets composed of different contents of neutral detergent fiber from Tifton hay (NDFf) in the dry matter of rations containing forage palm: D1: 11.98%; D2: 18.31%; D3: 23.68%; D4: 28.76% and D5: 32.12% NDFf. The intake of dry matter, crude protein, neutral detergent fiber, non-fiber carbohydrates and ether extract decreased linearly with the increase in NDFf content. There was a linear increase of milk production and milk protein with increasing dietary NDFf. The fat, dry extract, protein, pH and acidity of the evaluated milk were not affected by increasing the levels of NDFf in the diet. In situations where dairy goats are fed forage palm and other types of forage are limited, 11.98% NDF from forage other than palm can be used as an effective source of NDF.

scholarly journals Study of the Soil—Plant (Carrot)—Animal Cycle of Nutritive and Hazardous Minerals in a Rabbit Model

1999 ◽  
Vol 47 (2) ◽  
pp. 181-190 ◽  
Author(s):  
A. Bersényi ◽  
S. Fekete ◽  
I. Hullár ◽  
I. Kádár ◽  
M. Szilágyi ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Rabbit Model ◽  
Basal Diet ◽  
The Body ◽  
High Concentration ◽  
New Zealand White Rabbits ◽  
Heavy Metal Salts ◽  
Experimental Treatments ◽  
Rate Of Accumulation

Carrots were grown on soils polluted by heavy metal salts. Each particular microelement reached a high concentration [molybdenum (Mo) 39.00, cadmium (Cd) 2.30, lead (Pb) 4.01, mercury (Hg) 30.00, and selenium (Se) 36.20 mg/kg dry matter] in the carrot. In a metabolic balance trial conducted with 15 male and 15 female New Zealand White rabbits, the control animals (n = 5) were fed ad libitum with concentrate as basal diet, while the other rabbits received the basal diet and carrots containing the particular microelement. Blood samples were taken to determine the activity of serum enzymes. To investigate the metabolism of Mo, Cd, Pb, Hg and Se, samples were taken from the heart, liver, lungs, kidneys, spleen, ovaries/testicles, entire digestive tract, adipose tissue, femur, hair, faeces and urine. Carrot had significantly higher digestibility for all nutrients than the rabbit concentrate. Carrot samples of high Pb content had the lowest digestibility of crude protein. The microelements differed in their rate of accumulation in the organs examined: Mo and Cd accumulated in the kidneys, Pb in the kidneys, liver, bones and lungs, Hg in the kidneys and liver, while Se in the liver, kidneys and heart. The proportions of microelements eliminated from the body either via the faeces and urine (Mo 80.18% and Se 47.41%) or via the faeces (Cd 37.86%, Pb 66.39%, Hg 64.65%) were determined. Pathohistological examination revealed that the rate of spermatogenesis was reduced in the Mo, Cd, Pb and Hg groups compared to the control. Lead, Cd and Hg intake resulted in a considerable decrease in gamma-glutamyltransferase (GGT) and in an increase of alkaline phosphatase (ALP) activity because of damages to the kidneys and bones. All experimental treatments decreased the activity of cholinesterase (CHE) because of lesions in the liver.

The effect of different proportions of hay and fortified rolled barley in the diet on digestible-energy value and urinary-energy excretion by cattle

1972 ◽  
Vol 79 (1) ◽  
pp. 99-103 ◽  
Author(s):  
A. M. Raven
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Progressive Increase ◽  
Linear Increase ◽  
Metabolizable Energy ◽  
Digestible Energy ◽  
Gross Energy ◽  
Concentrate Treatment

SUMMARYA 6 x 6 Latin Square balance experiment was carried out using six Friesian steers, each of which initially weighed about 304 kg. The six treatments studied were an all-hay diet and five other diets containing 20,40,60,80 and 100 % of rolled barley fortified with mineral and vitamin supplements, accompanied by correspondingly reduced proportions of hay. Each diet was fed at an estimated maintenance level of feeding.The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in both digestible and calculated metabolizable energy. The actual increase in digestible energy was from 2·62Mcal/kg dry matter (59·3% of the gross energy) on the all-hay treatment to 3·42 Mcal/kg dry matter (79·5% of the gross energy) on the all-concentrate treatment. Use of the determined digestible energy values for the all-hay and fortified barley diets to calculate the digestible energy of the four mixed diets gave results in reasonably good agreement with the determined values, the maximum difference being 0·12 Mcal/kg dry matter, which represented 3·83 % of the determined value. The losses of energy in the urine expressed as percentages of the gross energy of the diets showed a small but significantly linear decrease (P < 0·01) with increase in proportion of barley in the diet. The molar proportions of steamvolatile acids in samples of rumen fluid taken from two animals on each treatment indicated that increase in the proportion of concentrate was associated with tendencies for increase in acetic acid, decrease in propionic acid and little change in butyric acid. The mean digestibility of the organic matter was 62·6 % on the all-hay treatment and 81·8 % on the all concentrate treatment. The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in digestibility of the organic matter. Although intakes of nitrogen decreased with increase in the proportion of concentrate due to a decrease in the amount of dry matter fed, the weights of nitrogen retained were well maintained and when expressed as percentages of intake showed a significantly linear increase (P < 0·01).

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