A MODIFIED METHOD FOR THE IN VITRO DETERMINATION OF DRY MATTER AND ORGANIC MATTER DIGESTIBILITY

1973 ◽  
Vol 53 (2) ◽  
pp. 251-256 ◽  
Author(s):  
R. E. LARSEN ◽  
G. M. JONES

Several modifications were made to the two-stage in vitro fermentation techniques for dry matter and organic matter digestibility (IVDMD and IVOMD) determinations developed by Tilley and Terry (J. Brit. Grassland Soc. 18: 104–111, 1963) and Alexander and McGowan (J. Brit. Grassland Soc. 21: 140–147, 1966). These modifications included: (1) changing the buffer medium, which resulted in a pH of 6.8–7.0 in the fermentation tubes during the initial (stage 1) 48-h fermentation period, and (2) shortening the acid-pepsin incubation period from 48 to 24 h, and thus reducing time for estimation of both IVDMD and IVOMD by 24 h. Modification (1) eliminated pH adjustments during fermentation and acidification at the end of the fermentation period. Acid-pepsin digestion of substrates was completed within 24 h instead of 48 h. Both IVDMD and IVOMD values were obtained on the same substrates. These modifications were evaluated using 65 samples that arose from 13 forages, comprised of corn and grass silages and their mixtures, which had been dried by five different methods. IVDMD and IVOMD were determined on all samples, comparing the modified method with the parent methods. IVDMD and IVOMD coefficients within each method were not statistically different between the Tilley and Terry method and the modified method.

2019 ◽  
Vol 12 (9) ◽  
pp. 1478-1483 ◽  
Author(s):  
Ezi Masdia Putri ◽  
Mardiati Zain ◽  
Lili Warly ◽  
Hermon Hermon

Aim: This research aimed to discover the chemical composition, as well as the content of the degradable and undegradable protein of the ruminant feed commonly used as cattle feed by Indonesian farmers. Materials and Methods: In this study, Pennisetum purpureum, Leucaena leucocephala, Indigofera zollingeriana, Gliricidia sepium, cassava, maize, palm kernel cake, and rice bran were used as feed. Chemical composition was determined by proximate and Van Soest analyses performed in triplicate. Dry matter and organic matter digestibility, as well as the rumen degradable proteins (RDP) and rumen undegradable proteins (RUP) contents, were determined in vitro using the Tilley and Terry method. Results: The results showed that more proteins can be obtained from legumes than from grass or concentrates. The highest protein amount was obtained from I. zollingerian (31.22%), while the lowest protein amount was obtained from cassava (3.59%). Dry matter digestibility ranged from 18.53% (rice bran) to 49.21% (G. sepium). Organic matter digestibility ranged from 35.71% (cassava) to 59.57% (I. zollingerian). Rice bran had the highest RDP from concentrate (73.26%), whereas I. zollingerian had the highest RDP from forage (74.72%). The highest RUP from concentrate was obtained from palm kernel cake (61.01%), and the highest RUP from forage was obtained from L. leucocephala (49.23%). Conclusion: The preparation of ruminant livestock rations must be based on RDP and RUP to meet the needs of both the rumen microbes and host animals. Information regarding the RDP and RUP of feeds is still limited, making this study useful in the preparation of ruminant livestock rations based on RDP and RUP ratios. Keywords: chemical compositions, rumen degradable proteins, rumen undegradable proteins, ruminant, tropical feedstuff.


2008 ◽  
Vol 8 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Muhamad Bata

The effect of molasses on ammoniated straw by using urea on dry and organic matter digestibility as in vitroABSTRACT. Aimed of this research was to find out the optimal level of molasses addition to improve quality, dry matter and organic matter digestibility of rice straw ammonization process. Materials used were rumen fluid of fistula cattle, grind of rice straw, water, urea and molasses. Research designed used Completely Randomized Design (CRD). As treatments were R0: rice straw 1000 g dry matter + 500 g water + 50 g urea + 0 percent of molasses, R1: R0 + 15 percent of molasses, R2: R0 + 30 percent of molasses. Urea and molasses dissolved in water and then entered into pollybag. All pollybag observe and let for 15 days, each treatment replicated 6 times. Variable measured were dry matter digestibility and organic matter digestibility. Research result showed that ammonization product of NH3, Acidity Level and crude fiber having decreased while crude protein content increased. Variance analysis indicated that treatments had significant effect (P0.05) on dry matter and organic matter digestibility. Orthogonal polynomial test indicated that level of molasses increase (P0.05) of dry matter and organic matter digestibility linearly. It can be concluded that addition up to 30 percent in ammoniating of rice straw using urea can improve quality of ammonization and increasing dry matter and organic matter digestibility.


2013 ◽  
Vol 13 (2) ◽  
pp. 59-67 ◽  
Author(s):  
Syapura Syapura ◽  
Muhamad Bata ◽  
Wardhana Surya Pratama

Improving of rice straw quality and its effect on ability nutrient digestibility and rumen metabolism products of buffalo in-vitro with feces as inoculum source ABSTRACT.  This study was aimed to determine the effect of feeding  ammoniated rice straw plus concentrate on buffalo nutrient digestibility and rumen fermentation products by in vitro. The Research was carried out by using  experimental method, designed according to completely  randomized design (CRD). The source of inoculum was obtain from different feces of three  buffalos kept in  Datar Village of Purwokerto region fed  rice straw, rice straw plus concentrate and rice straw ammoniated plus concentrate with dry matter ratio of 80 : 20. The treatments tested consisted of three treatments, namely R0 =  control feed using rice straw; R1 = the use of rice straw plus concentrate with a ratio of  (DM basis) 80:20; R2 = the use of ammoniated rice straw plus concentrate with a ratio of (DM basis) 80:20. The treatments were repeated 7 times, so there were 21 experimental units. The Variables measured included total VFA, Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS),   Dry Matter and Organic Matter Digestibility. The result of this study showed that the treatment had an effect significant (P0.05) on the concentration of VFA, Ratio A/P,  N-NH3,  Microbial Protein Synthesis (MPS), and Dry Matter and Organic Matter Digestibility. The HSD test showed that the highest production of  VFA,Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS), Dry Matter and Organic Matter Digestibility were achieved at R2 followed by R1 and R0 respectively. The conclusion is that the ammoniated rice straw supplemented with concentrate can be recommended to be fed to buffalo


2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques


2017 ◽  
Vol 2 (2) ◽  
pp. 1-6
Author(s):  
H. Husnaeni ◽  
M. Arifin Amril ◽  
Sjamsuddin Rasjid

This research aims to improve the digestibility and nutritional value of rice straw as a ruminant animal feed and determine the effect of long soaking in sea water of rice straw on digestibility in vitro dry matter and organic matter. The used materials in this study are a bales machine, weighing scales, rapiah rope, poly bags, rice straw varieties Ciliwung obtained from rice fields in Bantimurung, sea water with 2.71% saline, as well as tools and chemicals for analysis of in vitro dry matter and organic matter digestibility with pepsin, calculus method. This study is done based on Randomized Complete  Design (RCD) with 5 treatments and 4 replications. Variance based on the long soaking treatment gives the nature of the response curve linear on the digestibility of dry matter and also to organic matter digestibility. The magnitude of the correlation length relationship soaking rice straw and with sea water for in vitro dry matter and organic matter digestibility that is 96.5% and 96.8% respectively. Based on the results of research and discussion can be concluded that rice straw is soaked with sea water 3-12 days producing dry matter digestibility and organic matter are higher than the rice straw that is not soaked with sea water. Soaking of the sea water for 3-12 days increase the digestibility of dry matter and organic matter.


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