scholarly journals EFFECTS OF ENSILING UPON FREE AMINO ACIDS AND AMINES IN WHOLE-PLANT CORN, AND ON ITS SUBSEQUENT NUTRITIVE VALUE FOR LAMBS

1982 ◽  
Vol 62 (1) ◽  
pp. 259-267 ◽  
Author(s):  
L. E. PHILLIP ◽  
J. G. BUCHANAN-SMITH
Keyword(s):  
Amino Acids ◽  
Nutritive Value ◽  
Latin Square ◽  
N Balance ◽  
Nonprotein Nitrogen ◽  
Total N ◽  
Whole Plant ◽  
N Utilization ◽  
Voluntary Intake

In a replicated 2 × 2 double latin square, 16 lambs were fed whole-plant corn harvested at 26% or 38% dry matter (DM) and either ensiled or frozen. Corn was supplemented with urea (1.25%, DM basis). Voluntary intake was measured during a 17-day period of ad libitum feeding, after which the lambs were restricted on feed (65 g DM/Wkg0.75) for 14 days for the determination of nitrogen (N) balance and digestibility. Ensiling resulted in an increase in nonprotein nitrogen (NPN), mainly as amino acid-N, from 21% to 48% of the total N in the low DM corn, and from 25% to 43% in the high DM corn. Basic and acidic amino acids were selectively degraded during ensilage. Amine-N accounted for less than 5% of total N in the silages. Voluntary intake (g DM/Wkg0.75) of ensiled corn was not significantly different from that of frozen corn (77.3 vs. 81.7) but was higher (P < 0.05) for the low DM than the high DM corn (85.1 vs. 73.9). Estimates of N balance and digestibility of DM and organic matter were not affected (P < 0.05) by ensiling or by stage of harvest. There appears to be no adverse effect of ensiling whole-plant corn on its voluntary intake and N utilization by ruminants, provided the silage is supplemented with urea. Key words: corn, ensiling, intake, Digestibility, ruminants, nonprotein nitrogen

scholarly journals Comparison of the isotope dilution method for determination of the ileal endogenous amino acid losses with labelled diet and labelled pigs

2000 ◽  
Vol 83 (2) ◽  
pp. 123-130 ◽  
Author(s):  
Vincent Hess ◽  
Philippe Ganier ◽  
Jean-Noel Thibault ◽  
Bernard Sève
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Latin Square ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
First Case ◽  
Endogenous Protein ◽  
The Individual ◽  
Protein Free Diet

The aims of the present study were first to compare the amino acid dilution method performed using labelled animals with that using labelled diets, and second to determine real digestibilities and total ileal endogenous losses of N and amino acids. Two diets containing pea cultivars (Solara and Amino) and a protein-free diet were compared in a 3 × 3 Latin-square experiment. The three pigs were each prepared with an ileo-rectal anastomosis and were continuously infused with [1-13C]leucine. For each cultivar,15N-labelled and unlabelled diets were formulated. The real digestibility and endogenous losses of leucine were higher when obtained by labelling the pig than by labelling the foodstuff. This was due either to the inadequate estimation of the endogenous protein enrichment in the first case or to the importance of dietary N recycling in the second case. However, in both cases the ileal endogenous losses of N and amino acids were higher than the basal losses determined with the protein-free diet. There were significant differences between the two pea cultivars in terms of phenylalanine and leucine when measured with labelled diets. It is suggested that, although ileal endogenous losses may be underestimated, using labelled feedstuffs is of great interest due to the direct estimation of the individual amounts of amino acids.

Nutritive value of peas for lactating dairy cattle

2001 ◽  
Vol 81 (4) ◽  
pp. 541-551 ◽  
Author(s):  
G. R. Khorasani ◽  
E. K. Okine ◽  
R. R. Corbett ◽  
J. J. Kennelly
Keyword(s):  
Dairy Cows ◽  
Nutritive Value ◽  
Starch Content ◽  
Latin Square ◽  
Barley Grain ◽  
Linear Increase ◽  
Ruminal Fermentation ◽  
Total N ◽  
Ad Libitum Intake ◽  
Dietary Treatments

The objective of this study was to determine the nutritive value for lactating dairy cows of peas relative to soybean meal (SBM) and barley. Four Holstein cows (200 ± 23 d in milk), fitted with rumen and duodenal cannulae were assigned to four dietary treatments in an experiment designed as a 4 × 4 Latin square with 21 d in each of the four periods. Cows were fed a diet for ad libitum intake with a 50:50 forage:concentrate ratio (DM basis). Peas replaced SBM at the levels of 0, 33.3, 66.7%, and 100% of the concentrate portion in the four test diets. In the 100% pea-based diet, barley was replaced (at 72.35%) to obtain a similar starch content as the SBM-based concentrate. The forage components of the diets consisted of 25% alfalfa silage and 25% bromegrass silage. Dry matter intake (21.6 ± 0.4 kg d–1) and milk yield were not affected by substitution of peas for SBM and barley. Mean rumen pH decreased linearly (P < 0.01) with increasing level of peas in the diet. Substitution of peas for SBM and barley resulted in a linear increase in the concentration of rumen acetate, butyrate, isovalerate, and valerate (P < 0.01), and had a quadratic effect on caproate. Rumen bacterial yield and duodenal flow of total N were not affected by treatment. Ruminal fermentation characteristics and digestibility of nutrients suggest that the substitution of SBM and barley grain with peas may alter the site and end-products of digestion; however, the substitution had no significant effects on production parameters. Key words: Peas, digestion, rumen fermentation, dairy cows

Accretion of total protein and individual amino acids by organs and tissues of growing lambs and the ability of nitrogen balance techniques to quantitate protein retention

1993 ◽  
Vol 57 (2) ◽  
pp. 237-245 ◽  
Author(s):  
J. C. MacRae ◽  
A. Walker ◽  
D. Brown ◽  
G. E. Lobley
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Intestinal Tract ◽  
Live Weight ◽  
N Balance ◽  
Total Amino Acid ◽  
Total N ◽  
Comparative Slaughter ◽  
Total Body ◽  
Gastro Intestinal Tract

AbstractTwelve Suffolk-Finn Dorset lambs were reared from 25 to 40 or 25 to 55 kg body weight on either pelleted dried grass or a ration of pelleted grass plus barley (ratio 1:1) in a comparative slaughter experiment designed to determine the amounts of total nitrogen and individual amino acids accreted in different body components during growth. Nitrogen (N) balance measurements were determined frequently during this growth phase and accumulated N retentions were compared with the total N accretion determined by comparative slaughter. Total N and individual amino acids accumulated in carcass, wool, skin, offal and blood, head and feet, gastro-intestinal tract and liver were linearly related to body weight in all cases other than for cysteine in carcass. At 25 kg live weight, proportionately 0·52 of total body N was in carcass components, 0·115 in wool, 0·08 in skin, 0·10 in offal and blood, 0·095 in head and feet, 0·06 in the gastro-intestinal tract and 0·02 in liver. However as the animals grew from 25 to 55 kg, 0·256 of the total N accretion was in wool, which was rich in cysteine (98 g/kg total amino acid). Carcass accretion represented only 0·449 of total body N accretion. The N balance technique overestimated net protein accretion by 0·24 (s.e. 0·036).

scholarly journals The effect of cell wall degrading enzymes on the preservation of grass and on the silage intake and digestibility in sheep

1990 ◽  
Vol 62 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Seija Jaakkola
Keyword(s):  
Cell Wall ◽  
Dry Matter ◽  
Latin Square ◽  
Pilot Scale ◽  
Cell Wall Degrading Enzymes ◽  
Crude Fibre ◽  
Total N ◽  
Degrading Enzymes ◽  
Voluntary Intake ◽  
Forage Harvester

Two experiments were carried out to study the effects of cell wall degrading enzymes as silage additive. A primary growth of timothy was cut with a mower and picked up with a precision-chop forage harvester in Exp. I (early cut), and harvested as direct-cut with a precision-chop harvester in Exp. II (late cut). The additive treatments were in Exp. I: 1) unwilted formic acid (FA) applied as AIV II (4.5 l/t), 2) wilted untreated, 3) wilted FA (4 l/t), 4) wilted enzyme A (glucose oxidase (GO) + hemicellulase (HC) 150 ml/t + cellulase (C) 200 ml/t), 5) wilted enzyme B (HC 150 ml/t + C 200 ml/t); and in Exp. II: 1) untreated, 2) FA (4 l/t), 3) E200 (C 200 ml/t + GO), 4) E400 (C 400 ml/t + GO), 5) E800 (C 800 ml/t + GO). The rate of application of GO was 50 000 IU/t. The silages were ensiled in pilot scale silos (3 m3) and the voluntary intake and digestibility in sheep were determined in two experiments designed as a 5 x 5 Latin square. The use of enzymes decreased the fibre content of silages, mainly the cellulose fraction, as compared with FA and untreated silages. Enzyme silages were well preserved with a low pH (3.93—4.15), moderate ammonia N (72—119 g/kg total N) and no butyric acid. As compared with untreated silages (mean pH 4.6, ammonia N 131) the preservation was improved. The FA silages were also well preserved (pH 4.0, ammonia N 57) with more restricted fermentation than enzyme silages. FA and especially higher levels of enzymes increased the amount of effluent. In Exp. I, the digestibilities of dry matter (DM), organic matter (OM) and crude fibre were not significantly (P>0.05) affected by the silage treatment. In Exp. II, the digestibility of DM and OM decreased linearly (P

Determination of the methionine requirement of finishing double-muscled Belgian blue bulls with a three-step method

2002 ◽  
Vol 82 (1) ◽  
pp. 95-102
Author(s):  
E. Froidmont ◽  
Y. Beckers ◽  
A. Théwis
Keyword(s):  
Plasma Concentrations ◽  
Latin Square ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Net Energy ◽  
Step Method ◽  
N Utilization ◽  
Animal Growth ◽  
Total Amino Acids

A three-step technique was used to determine total amino acids (AA) and the first limiting AA requirements in finishing double-muscled Belgian Blue (dmBB) bulls. In a first experiment, three dmBB bulls (505 ± 21 kg) received a low metabolizable protein diet containing 25% meadow hay and 75% concentrate. Net energy supply was adequate for maximizing daily gains because of continuous infusion of dextrose into the duodenum. The intestinal apparent disappearance of essential AA (EAA) averaged 70.8% and was the lowest for histidine (61.3%) and the highest for arginine (79.9%). In a second experiment, four dmBB bulls (517 ± 16 kg) received the same diet supplemented with duodenal infusion of dextrose and four doses of Na-caseinate (17, 33, 50 and 66% of metabolizable dietary AA) in a 4 × 4 Latin square design. Nitrogen retention for the basal diet alone and the four increasing supplements of Na-caseinate averaged 61, 64, 74, 75 and 78 g d–1, respectively. A supply of 720 g d–1 of metabolizable AA was defined as optimising the N utilization for animal growth. Based on patterns of plasma concentrations, methionine and phenylalanine were probably the limiting AA. In a third experiment, five dmBB bulls (513 ± 60 kg) fed the basal diet received duodenal infusion of dextrose and AA, equivalent to the second dose in exp. 2 except for the supply of metabolizable methionine (12.8, 15.1, 17.6, 20.1, 22.6 and 25.1 g d–1) that varied in a 6 × 6 Latin square design with one missing animal. On the basis of N retention, the metabolizable methionine requirement was estimated to 22.8 g d–1 and corresponded to 360 mg of metabolizable methionine per gram of N retained. Key words: Ruminant, amino acid, duodenal infusion, requirement, plasma, nitrogen pollution

The influence of formic acid and formaldehyde additives and type of harvesting machine on the utilization of nitrogen in lucerne silages: 2. Changes in amino-acid composition during ensiling and their influence on nutritive value

1978 ◽  
Vol 91 (3) ◽  
pp. 717-725 ◽  
Author(s):  
T. N. Barry ◽  
D. N. Mundell ◽  
R. J. Wilkins ◽  
D. E. Beever
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Formic Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Aspartic Acid ◽  
Nutritive Value ◽  
Formaldehyde Treatment ◽  
Amino Acid Degradation ◽  
Voluntary Intake

SummaryLucerne was made into ten silages using either a flail or a precision-chop harvester with application of formaldehyde or a range of rates of formic acid during harvesting. Amino-acid analyses were done on the silages and on samples taken from the herbage at ensiling.In excess of 70% of aspartic acid, serine, glutamic acid, tyrosine, threonine, lysine, histidine and arginine were destroyed in untreated silages when a flail harvester was used and in excess of 50% when a precision-chop harvester was used. Glycine, leucine, iso-leucine, valine and methionine showed little change, with net losses or increases of up to 10%, whilst proline, cystine + cysteine and phenylalanine were intermediate between these two categories. Net synthesis of alanine and α and γ amino-butyric acids occurred in the untreated silages. Increasing rates of formic acid addition, and the use of the precision-chop harvester, reduced the loss of the amino acids which were extensively degraded in the untreated silages and minimized the increases in alanine and α and γ amino-butyric acids. Formaldehyde treatment also reduced amino-acid degradation, apart from apparently high losses of lysine, histidine and tyrosine. It was concluded, however, that these losses arose through problems of estimation in formaldehyde-treated silages following HCl hydrolysis.It is suggested that the most probable precursors for alanine and α and γ aminobutyric acids were respectively aspartic acid, threonine and glutamic acid. Voluntary intake and the utilization of silage nitrogen by young sheep were related to the net changes in amino acids involved in decarboxylation reactions, the best measures of this being the interconversions involving the formation of alanine and α and γ aminobutyric acids. Deamination reactions appeared to be of much less importance in limiting silage nutritive value than those involving decarboxylation.

scholarly journals 122 Nutrient digestibility of heat pretreated or multienzyme treated corn whole stillage for pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 107-107
Author(s):  
Kevin Jerez-Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Amino Acids ◽  
Nutritive Value ◽  
Direct Method ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Net Energy ◽  
Ileal Digestibility ◽  
Whole Stillage ◽  
Net Energy Value ◽  
Corn Ddgs

Abstract A study was conducted to determine standardized ileal digestibility (SID) of amino acids (AA) and net energy value (NE) for pigs of heat-pretreated or enzyme-predigested corn whole stillage (WS; slurry material that is dried into DDGS). Ten ileal-cannulated barrows (initial BW = 65.6 ± 3.5 kg) were fed 5 diets in a replicated 5 × 5 Latin square design. The diets were cornstarch-based, containing corn DDGS, untreated WS (C-WS), heat-pretreated WS (Heat-WS) or enzyme-predigested WS (Predigested-WS), and N-free diet. Digestibility of AA in feedstuffs was determined by the direct method. Energy digestibility in feedstuffs was determined by difference from the N-free diet. The WS was heat pretreated at 140 °C and 70 psi for 15 min. Predigestion of the WS was achieved by incubating with multienzyme that supplied xylanase, celullase, α-galactosidase at 2.4, 2.0 and 2.3 mg per gram of WS, respectively, for 12 h at 55 °C. On DM basis, DDGS, C-WS, Heat-WS, Predigested-WS contained 32.8, 30.8, 28.18, and 39.7% CP, 39.8, 51.0, 52.2 and 53.8% NDF, and 4.5, 4.6, 5.7 and 4.5% EE, respectively. The SID of Lys for C-WS (75.5%) was greater (P &lt; 0.05) than that for C-DDGS (67.4%) and Heat-WS (53.9%), but lower (P &lt; 0.05) than for Predigested-WS (84.1%). The NE value for C-WS (2,793 kcal/kg) did not differ from that of C-DDGS (2,668 kcal/kg DM). The NE value for C-WS was greater (P &lt; 0.05) than that for Heat-WS (1,834 kcal/kg DM) and lower than that for Predigested-WS (2,814 kcal/kg DM). In conclusion, enzymatic predigestion of WS increased its SID of Lys and NE value, and hence it can be an attractive technology to increase the nutritive value of corn DDGS for pigs. Heat pretreatment reduced SID of AA and NE values of the WS.

scholarly journals Some factors associated with the difference in nutritive value of artificially dried red clover and perennial ryegrass for sheep

1979 ◽  
Vol 42 (1) ◽  
pp. 139-147 ◽  
Author(s):  
G. Moseley ◽  
J. R. Jones
Keyword(s):  
Perennial Ryegrass ◽  
Nutritive Value ◽  
Latin Square ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Voluntary Intake ◽  
The Difference

1. Three sheep fitted with duodenal re-entrant cannulas and three with large rumen fistulas were given red clover, perennial ryegrass and a 2:1 (w/w) mixture of grass and clover in two Latin square arrangements. Measurements were made of voluntary intake, digestibility, flow of nutrients into the duodenum and the flow of Cr-EDTA marker through the reticulo-rumen.2. Organic matter (OM) digestibility was similar for the three feeds but the voluntary intake decreased in the order mixture > red clover > perennial ryegrass. There was an increase in the rate of marker flow from the rumen and a decrease in retention time of the same order. Rumen volume did not change significantly.3. There was a reduction in the mean particle size of rumen contents in the order perennial ryegrass > clover > mixture. The in vitro digestibility of particles decreased with size; the reduction being more rapid for clover than perennial ryegrass.4. The proportion of ingested digestible OM appearing at the duodenum increased from 18.4% to 26.7% to 30.0% for perennial ryegrass, clover and the mixture respectively.5. A higher proportion of digestible cellulose and hemicellulose disappeared over the stomach for the perennial ryegrass feed compared to the clover and the mixture but over 96% of water soluble carbohydrates and starch disappeared over the stomach for all three feeds.6. The apparent digestibility of nitrogen was similar for all three feeds but the proportion of undigested feed N appearing at the duodenum was calculated to be greater by a factor of 1.71 and 2.52 for clover and mixture feeds compared to grass.7. It was concluded that the higher nutritive value of red clover compared to perennial ryegrass was due to an increased rate of flow of nutrients througth the reticulo-rumen and an increase in the proportion of digestible OM digested post ruminally.

DETERMINATION OF THE METHIONINE REQUIREMENT OF GROWING PIGS USING SERUM FREE AMINO ACIDS

1972 ◽  
Vol 52 (1) ◽  
pp. 163-169 ◽  
Author(s):  
M. O. KEITH ◽  
D. A. CHRISTENSEN ◽  
B. D. OWEN
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Free Amino Acids ◽  
Free Amino ◽  
Latin Square ◽  
Growing Pigs ◽  
Performance Data ◽  
Serum Free ◽  
Methionine Concentration

Six littermate Yorkshire gilts averaging 18 kg body weight were fed a semipurified diet containing six graded levels of methionine during six 4-day feeding periods in a Latin square design. Serum methionine concentrations were determined at the end of each period. Plotting serum methionine concentration against dietary methionine intake showed the methionine requirement to be 0.46% of the diet; this estimate was substantiated by animal performance data.

WET CAGE LAYER EXCRETA ADDED TO WHOLE PLANT CORN PRIOR TO ITS ENSILING AND EFFECT ON FOOD INTAKE AND UTILIZATION BY SHEEP

1982 ◽  
Vol 62 (1) ◽  
pp. 163-172 ◽  
Author(s):  
J. G. BUCHANAN-SMITH ◽  
G. K. MACLEOD ◽  
J. C. JOFRIET
Keyword(s):  
Soybean Meal ◽  
Crude Protein ◽  
Nutritive Value ◽  
Nitrogen Retention ◽  
Corn Silage ◽  
Balance Study ◽  
Whole Plant ◽  
Key Words Animal ◽  
Voluntary Intake ◽  
Extensive Degradation

Corn ensiled with wet cage layer excreta was compared with untreated corn silage supplemented at feeding either with excreta or with soybean meal, in an intake assay and balance study using 25-kg lambs. Comparisons were made at both 10 and 20% inclusion levels (wt/wt, as-is basis) for excreta and to equivalent levels of crude protein from soybean meal, providing six treatments in all. Voluntary intake of dry matter for excreta-silage (mean – 56.8 g/kg0.75 body wt) was lower (P < 0.05) than for corn silage supplemented with excreta (mean – 80.7 g/kg0.75 body wt) which was comparable to the value obtained for silage supplemented with soybean meal. Digestibilities of dry and organic matter were significantly lower (P < 0.05) for all diets containing cage layer excreta compared to soybean meal. Nitrogen retention was lower (P < 0.05) for sheep fed silage supplemented with excreta rather than soybean meal, but was not adversely affected for sheep fed corn ensiled with excreta. Relative to untreated silage, addition of excreta at ensiling caused extensive degradation of free amino acids to amines and ammonia but did not enhance proteolysis. Absolute levels of five amines were increased by 46% in 10% excreta-silage and by 64% in 20% excreta-silage compared to untreated silage (163 mg amine-N/100 g DM). Low intake of excreta-silage may be explained by the amine levels associated with them but it is quite probable that several other factors were involved. Key words: Animal waste, ruminant nutrition, nutritive value, ensiling, corn

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