scholarly journals The effect of ante mortem electrolyte therapy on animal physiology and meat quality in pigs segregating at the halothane gene

1993 ◽  
Vol 73 (2) ◽  
pp. 231-240 ◽  
Author(s):  
A. L. Schaefer ◽  
A. C. Murray ◽  
A. K. W. Tong ◽  
S. D. M. Jones ◽  
A. P. Sather
Keyword(s):  
Slow Release ◽  
B Value ◽  
Heart Tissue ◽  
Pork Quality ◽  
Drip Loss ◽  
Tissue Levels ◽  
Beneficial Effects ◽  
Animal Physiology ◽  
Key Words Magnesium ◽  
Plasma Magnesium

The present study was initiated to investigate the effect of oral potassium (600 mg, slow release, 24 h preslaughter), intramuscular (IM) magnesium (500 mg MgSO4, 2 h preslaughter) or slow release magnesium (Magnesium-aspartate-hydrochloride (Mg Asp) 20 or 40 (g d−1 for 5 d) on animal physiology and pork quality. Experiments were undertaken on 318 barrows and gilts (100 kg ± 5 kg) represented by three genotypes segregating at the halothane locus (nn, Nn and NN genotypes). Experiment 1 indicated that the oral potassium effectively (P ≤ 0.05) reduced pCO2 values in the nn genotype. Intramuscular injectable magnesium treatments were also found to raise plasma magnesium (P < 0.05) but did not alter muscle, liver or heart tissue levels. Both oral potassium and i.m. magnesium exacerbated subjective pork structure and texture scores but were found to increase muscle brightness and hue (higher b* value). In exp. 2, oral magnesium-aspartate (Mg Asp) was found to raise plasma, but not tissue, levels of magnesium. When fed to a group of halothane carrier pigs (Nn genotype), Mg Asp reduced muscle temperature 45 min post-stunning, increased muscle redness (CIE a* value) and reduced muscle drip loss (P < 0.06). It was concluded that oral potassium and i.m. magnesium as administered in the present study had no beneficial effects on pork quality but that the beneficial effects of Mg Asp on meat colour and drip loss may warrant further investigation. Key words: Magnesium, potassium, preslaughter, pork quality, PSE pork

scholarly journals Beneficial Effects of Slow-Release Large Neutral Amino Acids after a Phenylalanine Oral Load in Patients with Phenylketonuria

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 4012
Author(s):  
Iris Scala ◽  
Daniela Concolino ◽  
Anna Nastasi ◽  
Giulia Esposito ◽  
Daniela Crisci ◽  
...  
Keyword(s):  
Amino Acids ◽  
Slow Release ◽  
Good Adherence ◽  
Large Neutral Amino Acids ◽  
Neutral Amino Acids ◽  
Beneficial Effects ◽  
Adherence To Diet ◽  
Low Protein ◽  
Single Oral Administration ◽  
Stable Plasma

The mainstay of phenylketonuria treatment is a low protein diet, supplemented with phenylalanine (Phe)-free protein substitutes and micronutrients. Adhering to this diet is challenging, and even patients with good metabolic control who follow the dietary prescriptions in everyday life ignore the recommendations occasionally. The present study explores the ability of slow-release large neutral amino acids (srLNAAs) to prevent Phe increase following a Phe dietary load. Fourteen phenylketonuric patients aged ≥13 years were enrolled in a 6-week protocol. Oral acute Phe loads of 250 and 500 mg were added to the evening meal together with srLNAAs (0.5 gr/kg). Phe and tyrosine were dosed before dinner, 2h-after dinner, and after the overnight fast. After oral Phe loads, mean plasma Phe remained stable and below 600 µmol/L. No Phe peaks were registered. Tyrosine levels significantly increased, and Phe/Tyrosine ratio decreased. No adverse events were registered. In conclusion, a single oral administration of srLNAAs at the dose of 0.5 gr/kg is effective in maintaining stable plasma Phe during acute oral loads with Phe-containing food and may be added to the dietetic scheme in situations in which patients with generally good adherence to diet foresee a higher than prescribed Phe intake due to their commitments.

scholarly journals Naringenin Release to Biomembrane Models by Incorporation into Nanoparticles. Experimental Evidence Using Differential Scanning Calorimetry

Surfaces ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 295-305
Author(s):  
Cristina Torrisi ◽  
Marco Di Guardia ◽  
Francesco Castelli ◽  
Maria Grazia Sarpietro
Keyword(s):  
Differential Scanning Calorimetry ◽  
Heart Tissue ◽  
Protective Role ◽  
Delivery Systems ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Drug Bioavailability ◽  
Cell Level ◽  
Scanning Calorimetry ◽  
Beneficial Effects

Naringenin (4′,5,7-trihydroxyflavanone-7-rhamnoglucosideor naringenin-7-rhamnoglucoside), a flavonoid present in large quantities in citrus, has different beneficial effects on human health as an antioxidant, free radical scavenger, anti-inflammatory, carbohydrate metabolism promoter, and immune system modulator. Different studies have shown that this substance also has a hypoglycemic and antihypertensive effect, reduces cholesterol and triglycerides, and plays an important protective role in the heart tissue; moreover, it provides neuroprotection against various neurological disorders such as Parkinson’s disease and unpredictable chronic stress-induced depression. Despite these advantages, Naringenin is poorly absorbed, and the small percentage absorbed is rapidly degraded by the liver, as a result losing its activity. Several approaches have been attempted to overcome these obstacles, among them, nanotechnology, with the use of Drug Delivery Systems (DDS) as Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC). DDS can, in fact, improve the drug bioavailability. The aim of this study was to develop and characterize SLN and NLC containing Naringenin and to evaluate the ability of these nanoparticles to release Naringenin at the cell level using biomembrane models represented by Multilamellar Vesicles (MLV). These studies were performed using Differential Scanning Calorimetry, a powerful technique to detect the interaction of drugs and delivery systems with MLV. It was shown that Naringenin could be better incorporated into NLC with respect to SLN and that Naringenin could be released by NLC into the biomembrane model. Therefore, suggesting the administration of Naringenin loaded into nanoparticles could help avoid the disadvantages associated with the use of the free molecule.

scholarly journals Increased Inclusion of Dietary Methionine Sources Improves Pork Quality in Association with the Regulation of Energy and Protein Metabolism and Muscle’s Fiber Profile

2020 ◽  
Author(s):  
Peiqiang Yuan ◽  
Sen Lin ◽  
Jiyong Peng ◽  
Yunxia Li ◽  
Yunhan Liu ◽  
...  
Keyword(s):  
Meat Quality ◽  
Protein Metabolism ◽  
Shear Force ◽  
Lipid Synthesis ◽  
Short Chain Fatty Acids ◽  
Pork Quality ◽  
Fiber Types ◽  
Drip Loss ◽  
Lower Shear ◽  
Nutritional Strategies

Abstract Background Intensive selection for faster growth rate and higher lean percentage led to increase in protein deposition but deterioration in meat quality of pigs, thus there is growing interest in exploring the nutritional strategies to improve meat quality. Methionine has been shown to activate mechanistic target of rapamycin complex 1 protein kinase that plays pivotal roles in the regulation of protein and lipid synthesis. However, few study reports are available regarding the effects of dietary methionine supplementation at levels beyond growth requirements on lipid and protein metabolism and thus on pork quality. The objective of this study was to assess whether pork quality was improved by increasing dietary digestible sulfur amino acids (SAA) levels, with pigs fed the control (100% SAA), DL-Methionine (125% SAA)- or OH-Methionine (125% SAA)-supplemented diets during 11–110 kg period. Results Increasing SAA above requirements did not significantly affect growth performance, whereas improved pork quality as indicated by the decreased drip loss and a tendency towards decrease in shear force of longissimus lumborum muscle. Moreover, fresh muscle from barrows fed OH-Methionine showed a higher lightness value compared with the control and DL-Methionine treatments. The relatively lower shear force might be explained by the decrease in crude protein and increase in glycolytic potential, while the decreased drip loss was associated with down-regulation of genes (like fast glycolytic IIx) regulating fiber types. The increased lightness value of fresh muscle from barrows fed OH-Met diets appeared to be associated with the increased lactate level, which can be further explained by the increased plasma short-chain fatty acids concentrations, up-regulated G-protein coupled receptor 43 activation and enhanced glucagon-like peptide 1 secretion. Conclusion Increased SAA consumption appeared to improve pork water-holding capacity and tenderness likely through regulation of energy and protein metabolism and muscle’s fiber profile, which provides new insights into the nutritional strategies to improve meat quality.

scholarly journals Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice

2020 ◽  
Vol 11 ◽  
pp. 204062232097483
Author(s):  
Qing-Qing Zhang ◽  
Guo-Qing Li ◽  
Yi Zhong ◽  
Jie Wang ◽  
An-Ning Wang ◽  
...  
Keyword(s):  
Myocardial Dysfunction ◽  
Sglt2 Inhibitor ◽  
Heart Tissue ◽  
Left Ventricular ◽  
Tissue Samples ◽  
Beneficial Effects ◽  
Reduced Body ◽  
Sodium Glucose Cotransporter ◽  
And Function ◽  
Visceral Adipose

Background: Chronic exposure to excess glucocorticoids is frequently associated with a specific cardiomyopathy. Empagliflozin, a sodium-glucose cotransporter 2 (SGLT2) inhibitor, has beneficial effects as it aids in the reduction of heart failure and cardiovascular mortality in hospitalized patients. The aim of this study was to investigate the effects of empagliflozin on chronic hypercortisolism-induced myocardial fibrosis and myocardial dysfunction in mice. Methods: Male C57BL/6J mice (6 weeks old) were randomized to control, corticosterone (CORT), and empagliflozin + CORT groups. After 4 weeks of administration, heart structure and function were evaluated by echocardiography, and peripheral blood and tissue samples were collected. Expressions of Ccl2, Itgax, Mrc1, and Adgre1 mRNA in heart tissue were evaluated by RT-PCR, and signal transducer and activator of transcription 3 (STAT3) and Toll-like receptor 4 (TLR4) protein expression were analyzed by Western blotting. Results: Empagliflozin effectively reduced body weight, liver triglyceride, visceral adipose volume, and uric acid in CORT-treated mice. Left ventricular hypertrophy and cardiac dysfunction were improved significantly, phosphorylated STAT3 and TLR4 were alleviated, and macrophage infiltration in the myocardium was inhibited after administration of empagliflozin in CORT-treated mice. Conclusion: Empagliflozin has beneficial effects on specific cardiomyopathy associated with CORT, and the results provide new evidence that empagliflozin might be a potential drug for the prevention of this disease.

scholarly journals Effect of Pre- and Post-Plant Fungicide and Fertilizer Treatments on Infection by Colletotrichum acutatum, Plant Survival, and Yield of Annual Strawberry in Florida

2010 ◽  
Vol 11 (1) ◽  
pp. 30 ◽  
Author(s):  
James C. Mertely ◽  
Teresa E. Seijo ◽  
Natalia A. Peres
Keyword(s):  
Hydrogen Peroxide ◽  
Slow Release ◽  
Colletotrichum Acutatum ◽  
Kaolin Clay ◽  
Plant Mortality ◽  
Plant Survival ◽  
Beneficial Effects ◽  
Handling Practices ◽  
Spraying Plants ◽  
And Control

The effects of pre-plant dips and post-plant treatments on the infection by Colletotrichum acutatum, survival, and yields of strawberry plants were evaluated over two seasons in Florida using naturally infected transplants imported from northern nurseries. Handling practices such as washing, refrigerating, and dipping plants in water had no effect on infection, survival, or yield. Pre-plant dips with the fungicide Switch 62.5 WDG (cyprodinil + fludioxonil) and, in some cases, with Abound 2.08F (azoxystrobin), reduced infection and plant mortality, and increased yield. Pre-plant dips with Oxidate (hydrogen peroxide) were ineffective and, in some cases, increased plant mortality and reduced yield. Fertilizer treatments, such as dipping and spraying plants with Brotomax (8-0-0 plus micronutrients) or application of slow-release Osmocote (15-9-12 plus micronutrients) after planting, had no effect or reduced plant survival and yield. Post-plant application of Surround WP, a kaolin clay intended to reduce heat stress and control mites and insects, had little or no effect on infection, survival, or yield. In summary, strawberry transplants infected with C. acutatum can be dipped in Switch or Abound at planting to reduce infection and increase survival and yield, but other treatments and practices examined are unlikely to have beneficial effects. Accepted for publication 6 October 2009. Published 25 January 2010.

scholarly journals The effect of transport density and gender on stress indicators and carcass and meat quality in pigs

2015 ◽  
Vol 13 (3) ◽  
pp. e0606 ◽  
Author(s):  
Thuanny L. Pereira ◽  
Anderson Corassa ◽  
Cláudia M. Komiyama ◽  
Cláudio V. Araújo ◽  
Alessandra Kataoka
Keyword(s):  
Lactate Dehydrogenase ◽  
Meat Quality ◽  
Pork Quality ◽  
Drip Loss ◽  
Finishing Pigs ◽  
Blood Components ◽  
Stress Indicators ◽  
Ph Values ◽  
And Gender

<p>A total of 168 finishing pigs were used to investigate the effects of gender (barrows and gilts) and transport densities for slaughter (236, 251, and 275 kg/m²) on stress indicators and carcass and pork quality. The animals transported at 251 kg/m² (T251) presented cortisol values below those at 236 kg/m<sup>2</sup> (T236), but no different from those at 275 kg/m<sup>2</sup> (T275). The lactate dehydrogenase (LDH) values in pigs transported at T236 were the lowest. The blood components did not differ between T236 and T275. The pH values at 45 min (pH<sub>45</sub>) and at 24 h (pH<sub>24</sub>) <em>postmortem </em>were higher for pigs subjected to T236. However, the pH<sub>45 </sub>was higher at T251 than at T275, but pH<sub>24</sub> was lower at T251 than at T275. The lightness values in the muscles of the pigs transported at T236 and T251 were higher than those at T275. Lower drip loss values were observed in the muscle of animals at T251. Carcasses of pigs at T236 contained more 1–5 cm lesions while those at T275 contained more 5–10 cmlesions in sections of loin. No significant effects of gender were found on the stress indicators, blood components, pH<sub>45</sub>, pH<sub>24</sub>, color, drip loss or carcass lesions in general. These results indicate that the pre-slaughter transport of pigs at densities of 251 kg/m² generates less physiological damage and smaller losses on carcass and pork quality irrespective of gender.</p>

Effects of thyroid hormones on cardiac development in oculo

1993 ◽  
Vol 264 (1) ◽  
pp. H244-H251 ◽  
Author(s):  
A. Torres ◽  
D. C. Tucker
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Slow Release ◽  
Cardiac Development ◽  
Sympathetic Innervation ◽  
Heart Tissue ◽  
Heart Weight ◽  
Cardiac Growth ◽  
Beating Rate ◽  
Whole Heart

Embryonic rat myocardium was grafted into the anterior eye chamber (in oculo) of adult host rats in a series of two experiments that studied the effects of thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] on heart tissue developing without hemodynamic load. In each experiment, surgical sympathectomy of one eye chamber was used to define possible interactions between thyroid hormones and sympathetic innervation to the graft. In the first experiment, propylthiouracil (PTU, 20 mg/kg sc) greatly suppressed growth and beating rate of whole heart grafts, while excess T4 (0.1 mg/kg sc) transiently increased beating rate but not growth. In the second experiment, T3 (5 mg/21 days, slow-release pellet) failed to promote growth in ventricular grafts. However, the size of atrial grafts in surgically sympathectomized eye chambers was larger in T3-treated compared with control rats. T3 administration increased beating rate in ventricular grafts. In both experiments, thyroid hormone treatments were effective in increasing heart weight-to-body weight ratios in the host rats. The data suggest that exposure to normal levels of thyroid hormones may be necessary for optimal cardiac growth. The data also support the hypothesis that a hemodynamic load is required for thyroid hormone-induced cardiac growth.

The effects of feeding conjugated linoleic acid on subsequent pork quality

1999 ◽  
Vol 79 (1) ◽  
pp. 45-51 ◽  
Author(s):  
M. E. R. Dugan ◽  
J. L. Aalhus ◽  
L. E. Jeremiah ◽  
J. K. G. Kramer ◽  
A. L. Schaefer
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Shear Force ◽  
Detrimental Effect ◽  
Intramuscular Fat ◽  
Pork Quality ◽  
Drip Loss ◽  
Protein Levels ◽  
Flavor Intensity ◽  
Glycogen Utilization

Feeding conjugated linoleic acid (CLA) has recently been shown to repartition fat to lean in pigs. The present study was undertaken to determine if feeding CLA affects pork quality. Pigs were fed a cereal-based diet containing either 2% CLA or 2% sunflower oil. Fifty-four pigs (27 gilts and 27 barrows) were fed per diet, and diets were fed from 61.5 to 106 kg liveweight. Diet did not affect postmortem longissimus thoracis (LT) glycogen utilization, lactate accumulation, or pH decline. Conjugated linoleic acid fed pigs had slightly higher LT temperatures at 3 h postmortem (+1.15 °C; P < 0.05), but subsequent LT shear force, drip loss and soluble protein levels were unaffected. Diet did not affect subjective LT scores for structure or color, but objective color measurements indicated LT from CLA-fed pigs had slightly higher chroma (color saturation) values (+0.84; P < 0.05). Longissimus thoracis from CLA-fed pigs also had increased subjective marbling scores (P < 0.01) and increased petroleum-ether-extractable intramuscular fat (+22%; P < 0.01). Diet did not affect any measured palatability characteristic (initial and overall tenderness, juiciness, flavor desirability, flavor intensity, connective tissue amount, overall palatability; P > 0.05). Feeding 2% dietary CLA to pigs, therefore, shows some potential for improving pork composition by increasing intramuscular fat, while having no detrimental effect on pork quality. Key words: Swine, conjugated linoleic acid, pork quality

scholarly journals Technological and sensory pork quality in relation to muscle and drip loss protein profiles

2012 ◽  
Vol 234 (5) ◽  
pp. 883-894 ◽  
Author(s):  
Elżbieta Żelechowska ◽  
Wiesław Przybylski ◽  
Danuta Jaworska ◽  
Véronique Santé-Lhoutellier
Keyword(s):  
Pork Quality ◽  
Drip Loss ◽  
Protein Profiles

scholarly journals Effect of caffeine on zinc absorption and Zn concentration in rat tissue

1990 ◽  
Vol 64 (2) ◽  
pp. 553-559 ◽  
Author(s):  
Magdalena J. Rossowska ◽  
Tetsuo Nakamoto
Keyword(s):  
Femoral Vein ◽  
Direct Injection ◽  
Heart Tissue ◽  
Female Rats ◽  
Zinc Absorption ◽  
Contrast Injection ◽  
Tissue Levels ◽  
Zn Concentration ◽  
Rat Tissue ◽  
Different Doses

The purposes of the present study were to determine whether caffeine has an effect on zinc absorption and tissue levels of Zn. Under anaesthesia, one side of the intestine of female rats was connected to infusion pumps and an infused solution (first caffeine and later Zn solution) was collected from the other side of the intestine using either 300 mm or the whole small intestine to determine Zn absorption. In a further study, different doses of caffeine solution were injected directly into the femoral vein and a saline or Zn solution was infused into the intestine to measure Zn absorption and tissue Zn concentrations. The results consistently showed that the caffeine solution infused into the intestine did not affect intestinal Zn absorption nor was absorption affected by the direct injection of caffeine into the vein. In contrast, injection of different doses of the caffeine solution significantly decreased Zn tissue levels for the heart only. Calcium concentrations in the heart tissue were also decreased, but not magnesium concentrations. Tissue Zn levels recovered immediately on infusion of a Zn solution into the intestine.

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