In vitro testing of the hemaPEN microsampling device for the quantification of acetaminophen in human blood

Bioanalysis ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 1725-1737
Author(s):  
Arundhuti Sen ◽  
Molly Gillett ◽  
Lucy Weaver ◽  
Matthew Barfield ◽  
Pawanbir Singh ◽  
...  
Keyword(s):  
Human Blood ◽  
Dried Blood Spots ◽  
Extraction Process ◽  
User Training ◽  
Accuracy And Precision ◽  
Volumetric Accuracy ◽  
Performance Results ◽  
The Impact ◽  
And Storage

Background: The hemaPEN is a liquid microsampling device for the reproducible collection and storage of blood samples as dried blood spots, for subsequent quantitative analysis. Materials & methods: We examined the device’s ability to collect accurate and precise blood volumes, at different hematocrit levels, via in vitro studies using acetaminophen in human blood. We also investigated the impact of different user training approaches on device performance. Results: The hemaPEN demonstrated acceptable volumetric accuracy and precision, regardless of the training medium used. Issues with apparent hematocrit-dependent bias were found to be associated with the extraction process, rather than the volumetric performance of the device. Conclusion: The hemaPEN is capable of readily producing high quality blood microsamples for reproducible and accurate quantitative bioanalysis.

scholarly journals Influence of Pasteurization and Storage on Dynamic In Vitro Gastric Digestion of Milk Proteins: Quantitative Insights Based on Peptidomics

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 998 ◽  
Author(s):  
Xing Li ◽  
Yuxiang Gu ◽  
Shudong He ◽  
Olayemi Eyituoyo Dudu ◽  
Qiming Li ◽  
...  
Keyword(s):  
Cold Storage ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Milk Proteins ◽  
Hydrolysis Rate ◽  
Gastric Digestion ◽  
Pasteurized Milk ◽  
The Impact ◽  
And Storage

It is important to evaluate the nutritional quality of milk during the shelf-life, especially during home storage, from a consumer viewpoint. In this study, we investigated the impact of pasteurization (85 °C/15 s) and subsequent storage (at 4 °C for 7 days) on the coagulation behavior of milk and protein digestibility in a dynamic in vitro gastric digestion test. A high level of hydration in curd formed in pasteurized milk upon 7-day cold storage compared to raw and pasteurized milk, indicating fast pepsin diffusion in the interior of curds, increasing the hydrolysis rate. The digesta collected at various time points throughout the gastric digestion were studied using o-phthaldialdehyde (OPA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), liquid chromatography tandem mass spectrometry (LC-MS/MS), and amino acid analysis. These results showed that milk proteins were hydrolyzed quickly upon a long period of cold storage. Additionally, qualitative and quantitative results obtained using LC-MS/MS exhibited significant differences between samples, especially in pasteurized milk upon cold storage. Processing and storage played a decisive role in bioactive peptide generation. Such knowledge could provide insights into and directions for the storage of pasteurized milk for further clinical studies on protein bioavailability and the generation of bioactive peptides for desired health outcomes.

Impact Deposition of Radon Progeny (218Po ,214Po and 210Po) on the Surface of Human Blood: In Vitro Study

2011 ◽  
Vol 110-116 ◽  
pp. 2004-2009 ◽  
Author(s):  
Asaad Hamid Ismail ◽  
Mohamad S. Jaafar
Keyword(s):  
Human Blood ◽  
Radon Concentration ◽  
Absorbed Dose ◽  
Blood Component ◽  
Good Efficiency ◽  
Present Design ◽  
Human Blood Samples ◽  
The Impact ◽  
Exposure Technique

This work presented new irradiation technique to estimate the impact of radon's progeny deposition on the human blood surface on some blood diseases, using CR-39Nuclear Track Detectors (NTDs). The results show that the present design has good efficiency and the loss rate of radon concentration was a little. Amount of the loss of radon concentration during the process of mixing blood component did not affect on the efficiency of exposure technique. Therefore, method of mixing blood component process was successful. As well as, human blood exposure to radon gas (2210±5.1Bq/m3) make thrombocytopenia, and no effect on red blood cell. And rate of radon absorbed dose into the human blood samples is high at 20 minutes.

Thermodynamic Properties of Rifampicin Redox Current Peaks in Human Blood Samples Using Nano-Sensor (Carbon Nanotubes / Glassy Carbon Electrode)

2021 ◽  
Vol 43 (1) ◽  
pp. 41-41
Author(s):  
Sura Ali AL ASADI Sura Ali AL ASADI ◽  
Muhammed Mizher RADHI and Wisam Hindawi HOIDY Muhammed Mizher RADHI and Wisam Hindawi HOIDY
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Human Blood ◽  
Electrochemical Technique ◽  
Activation Entropy ◽  
Carbon Electrode ◽  
Different Temperatures ◽  
Human Blood Samples ◽  
The Impact

The cyclic voltammetric electrochemical technique was utilized to investigate the effect of different temperatures on the redox current peaks of rifampicin (RF), a drug commonly used to treat many diseases including tuberculosis (TB), in vitro for human blood medium. A modified working electrode of glassy carbon electrode (GCE) with carbon nanotube (CNT) (CNT / GCE) was used as a sensitive nano-sensor to evaluate the impact of temperature on the blood medium in the presence of RF ions. The results confirmed the presence of two oxidation and one reduction current peaks of RF in blood medium at 0.5, 1, and -0.5 V respectively. The redox current peaks of RF ions in blood medium were enhanced with increasing the temperature from 20 to 36oC. The activation energy (E*) values were determined by applying Arrhenius equation with oxidative and anti-oxidative peaks of Ea*(Ipa)= 9.252 and 11.026 kJ.mol-1.K-1,respectively. Other thermodynamic functions such as the change in each of activation Enthalpy (ΔH*), activation Gibbs energy (ΔG*) and activation Entropy (ΔS*) values were estimated using Eyring equation. The present results of the effects of different temperatures on the blood status in presence of RF lead to the explanation of the oxidative stress of the drug which used in an inflammatory of blood at different temperature.

scholarly journals THE IMPACT OF FRUCTOSE THE GROWTH AND STORAGE OF GRAPEVINE COLLECTION IN THE IN VITRO

2020 ◽  
Vol 6 (66) ◽  
pp. 184-197
Author(s):  
Natalia Petrovna Dorochenko ◽  
◽  
Valentina Georgievna Puzyrnova ◽  
Keyword(s):  
The Impact ◽  
And Storage

scholarly journals Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma

2021 ◽  
Vol 22 (19) ◽  
pp. 10614
Author(s):  
Eva Korábková ◽  
Věra Kašpárková ◽  
Daniela Jasenská ◽  
Dita Moricová ◽  
Eliška Daďová ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Body Fluids ◽  
Human Blood Plasma ◽  
Tio2 Particles ◽  
Commercial Mixture ◽  
Increased Risk ◽  
The Impact

The growing application of materials containing TiO2 particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO2 still exists. In this work, TiO2 particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO2 in these media were determined. The time dependent agglomeration of TiO2 related to the type of TiO2, and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO2 agglomeration. TiO2 was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO2 behavior in all the abovementioned environments is critical when TiO2 safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity.

scholarly journals The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 182 ◽  
Author(s):  
Anastasia Mantziari ◽  
Satu Tölkkö ◽  
Artur C. Ouwehand ◽  
Eliisa Löyttyniemi ◽  
Erika Isolauri ◽  
...  
Keyword(s):  
Human Milk ◽  
Liquid Scintillation ◽  
Adhesion Properties ◽  
Protein Supplements ◽  
Donor Human Milk ◽  
Probiotic Strains ◽  
Human Milk Fortifier ◽  
The Impact ◽  
And Storage

Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.

scholarly journals Impact of storage media and temperature on color stability of tooth-colored CAD/CAM materials for final restorations

2019 ◽  
Vol 17 (4) ◽  
pp. 228080001983683 ◽  
Author(s):  
Anja Liebermann ◽  
Dirk Vehling ◽  
Marlis Eichberger ◽  
Bogna Stawarczyk
Keyword(s):  
Red Wine ◽  
Storage Temperature ◽  
Resin Composite ◽  
Distilled Water ◽  
Storage Duration ◽  
Cad Cam ◽  
The Impact ◽  
And Storage ◽  
Storage Solution

Background: This in-vitro study examined the impact of storage solution, storage duration, and storage temperature on discoloration of three tooth-colored CAD/CAM materials for final restorations. Methods: Specimens ( N = 288; n = 96 per material) with a thickness of 1 ± 0.03 mm of the following CAD/CAM materials were fabricated: resin composite (Lava Ultimate, 3M), polymer-infiltrated ceramic (VITA Enamic, VITA Zahnfabrik), and leucite ceramic (IPS Empress CAD, Ivoclar Vivadent). After baseline measurement, specimens were stored in red wine, curry solution, cress solution, and distilled water at 37°C or 55°C. The discoloration was measured using a spectrophotometer (Lambda 35 Perkin Elmer, Perkin Elmer Inc.) after 1 and 7 days storage. Data were analyzed using four-way ANOVA followed by the Scheffé post-hoc test and partial eta squared ( ηP²) test ( p < 0.05). Results: The highest influence on ∆ E was exerted by storage duration ( ηP² = 0.295, p < 0.001), followed by storage solution ( ηP² = 0.171, p < 0.001), CAD/CAM material ( ηP² = 0.049, p < 0.001), and storage temperature ( ηP² = 0.033, p < 0.001). Specimens stored for 7 days in staining solutions showed more discoloration than those stored for just 1 day. Higher ∆ E values were achieved for specimens stored in curry solution, followed by red wine, cress solution, and distilled water. Resin composite Lava Ultimate showed larger ∆ E values compared with the resin hybrid ceramic VITA Enamic and leucite ceramic IPS Empress CAD. Specimens stored at 37°C showed significantly less discoloration than those stored at 55°C. Conclusions: The degree of coloration of the materials depends on food and temperature and was most pronounced with Lava Ultimate.

scholarly journals Borrelia burgdorferi Organisms Lacking Plasmids 25 and 28-1 Are Internalized by Human Blood Phagocytes at a Rate Identical to That of the Wild-Type Strain

2005 ◽  
Vol 73 (9) ◽  
pp. 5547-5553 ◽  
Author(s):  
Samiya Al-Robaiy ◽  
Jens Knauer ◽  
Reinhard K. Straubinger
Keyword(s):  
Borrelia Burgdorferi ◽  
Human Blood ◽  
Fluorescein Isothiocyanate ◽  
Blood Monocytes ◽  
Wild Type ◽  
Phagocytic Cells ◽  
Linear Plasmids ◽  
High Passage ◽  
The Impact

ABSTRACT Lyme borreliosis caused by Borrelia burgdorferi is a persistent infection capable of withstanding the host's vigorous immune response. Several reports have shown that the spirochete's linear plasmids 25 and 28-1 are essential for its infectivity. In this context, it was proposed that Borrelia burgdorferi organisms control their uptake by macrophages and polymorphonuclear leukocytes (PMNs) through plasmid-encoded proteins and that this mechanism confers resistance to phagocytosis. To investigate this proposal, a precise flow-cytometry-based method with human blood was used to study the impact of the plasmids 25 and 28-1 on B. burgdorferi clearance over 150 min and to investigate whether low-passage organisms are more resistant to phagocytosis than high-passage B. burgdorferi. Exposure of human blood PMNs or blood monocytes to fluorescein isothiocyanate-labeled B. burgdorferi B31 organisms lacking the linear plasmids 25, 28-1, or both revealed that all spirochete populations were internalized at the same rate as the wild-type borrelia parent strain B31. Moreover, no differences in phagocytosis kinetics were detected when low- or high-passage wild-type B. burgdorferi B31 or N40 were cocultured with blood cells. Plasmid loss and probable associated surface protein changes due to serial in vitro propagation of B. burgdorferi do not affect the resistance of these organisms to internalization by phagocytic cells. In particular, we found no evidence for a plasmid-controlled (lp25 and lp28-1) resistance of B. burgdorferi to phagocytosis by leukocytes of the host's innate immune system.

Thermodynamic Properties of Rifampicin Redox Current Peaks in Human Blood Samples Using Nano-Sensor (Carbon Nanotubes / Glassy Carbon Electrode)

2021 ◽  
Vol 43 (1) ◽  
pp. 41-41
Author(s):  
Sura Ali AL ASADI Sura Ali AL ASADI ◽  
Muhammed Mizher RADHI and Wisam Hindawi HOIDY Muhammed Mizher RADHI and Wisam Hindawi HOIDY
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Human Blood ◽  
Electrochemical Technique ◽  
Activation Entropy ◽  
Carbon Electrode ◽  
Different Temperatures ◽  
Human Blood Samples ◽  
The Impact

The cyclic voltammetric electrochemical technique was utilized to investigate the effect of different temperatures on the redox current peaks of rifampicin (RF), a drug commonly used to treat many diseases including tuberculosis (TB), in vitro for human blood medium. A modified working electrode of glassy carbon electrode (GCE) with carbon nanotube (CNT) (CNT / GCE) was used as a sensitive nano-sensor to evaluate the impact of temperature on the blood medium in the presence of RF ions. The results confirmed the presence of two oxidation and one reduction current peaks of RF in blood medium at 0.5, 1, and -0.5 V respectively. The redox current peaks of RF ions in blood medium were enhanced with increasing the temperature from 20 to 36oC. The activation energy (E*) values were determined by applying Arrhenius equation with oxidative and anti-oxidative peaks of Ea*(Ipa)= 9.252 and 11.026 kJ.mol-1.K-1,respectively. Other thermodynamic functions such as the change in each of activation Enthalpy (ΔH*), activation Gibbs energy (ΔG*) and activation Entropy (ΔS*) values were estimated using Eyring equation. The present results of the effects of different temperatures on the blood status in presence of RF lead to the explanation of the oxidative stress of the drug which used in an inflammatory of blood at different temperature.

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