THE IMPACT OF FRUCTOSE THE GROWTH AND STORAGE OF GRAPEVINE COLLECTION IN THE IN VITRO

It is important to evaluate the nutritional quality of milk during the shelf-life, especially during home storage, from a consumer viewpoint. In this study, we investigated the impact of pasteurization (85 °C/15 s) and subsequent storage (at 4 °C for 7 days) on the coagulation behavior of milk and protein digestibility in a dynamic in vitro gastric digestion test. A high level of hydration in curd formed in pasteurized milk upon 7-day cold storage compared to raw and pasteurized milk, indicating fast pepsin diffusion in the interior of curds, increasing the hydrolysis rate. The digesta collected at various time points throughout the gastric digestion were studied using o-phthaldialdehyde (OPA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), liquid chromatography tandem mass spectrometry (LC-MS/MS), and amino acid analysis. These results showed that milk proteins were hydrolyzed quickly upon a long period of cold storage. Additionally, qualitative and quantitative results obtained using LC-MS/MS exhibited significant differences between samples, especially in pasteurized milk upon cold storage. Processing and storage played a decisive role in bioactive peptide generation. Such knowledge could provide insights into and directions for the storage of pasteurized milk for further clinical studies on protein bioavailability and the generation of bioactive peptides for desired health outcomes.

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The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

Nutrients ◽

10.3390/nu12010182 ◽

2020 ◽

Vol 12 (1) ◽

pp. 182 ◽

Cited By ~ 1

Author(s):

Anastasia Mantziari ◽

Satu Tölkkö ◽

Artur C. Ouwehand ◽

Eliisa Löyttyniemi ◽

Erika Isolauri ◽

...

Keyword(s):

Human Milk ◽

Liquid Scintillation ◽

Adhesion Properties ◽

Protein Supplements ◽

Donor Human Milk ◽

Probiotic Strains ◽

Human Milk Fortifier ◽

The Impact ◽

And Storage

Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.

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Impact of storage media and temperature on color stability of tooth-colored CAD/CAM materials for final restorations

Journal of Applied Biomaterials & Functional Materials ◽

10.1177/2280800019836832 ◽

2019 ◽

Vol 17 (4) ◽

pp. 228080001983683 ◽

Cited By ~ 1

Author(s):

Anja Liebermann ◽

Dirk Vehling ◽

Marlis Eichberger ◽

Bogna Stawarczyk

Keyword(s):

Red Wine ◽

Storage Temperature ◽

Resin Composite ◽

Distilled Water ◽

Storage Duration ◽

Cad Cam ◽

The Impact ◽

And Storage ◽

Storage Solution

Background: This in-vitro study examined the impact of storage solution, storage duration, and storage temperature on discoloration of three tooth-colored CAD/CAM materials for final restorations. Methods: Specimens ( N = 288; n = 96 per material) with a thickness of 1 ± 0.03 mm of the following CAD/CAM materials were fabricated: resin composite (Lava Ultimate, 3M), polymer-infiltrated ceramic (VITA Enamic, VITA Zahnfabrik), and leucite ceramic (IPS Empress CAD, Ivoclar Vivadent). After baseline measurement, specimens were stored in red wine, curry solution, cress solution, and distilled water at 37°C or 55°C. The discoloration was measured using a spectrophotometer (Lambda 35 Perkin Elmer, Perkin Elmer Inc.) after 1 and 7 days storage. Data were analyzed using four-way ANOVA followed by the Scheffé post-hoc test and partial eta squared ( ηP²) test ( p < 0.05). Results: The highest influence on ∆ E was exerted by storage duration ( ηP² = 0.295, p < 0.001), followed by storage solution ( ηP² = 0.171, p < 0.001), CAD/CAM material ( ηP² = 0.049, p < 0.001), and storage temperature ( ηP² = 0.033, p < 0.001). Specimens stored for 7 days in staining solutions showed more discoloration than those stored for just 1 day. Higher ∆ E values were achieved for specimens stored in curry solution, followed by red wine, cress solution, and distilled water. Resin composite Lava Ultimate showed larger ∆ E values compared with the resin hybrid ceramic VITA Enamic and leucite ceramic IPS Empress CAD. Specimens stored at 37°C showed significantly less discoloration than those stored at 55°C. Conclusions: The degree of coloration of the materials depends on food and temperature and was most pronounced with Lava Ultimate.

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In vitro testing of the hemaPEN microsampling device for the quantification of acetaminophen in human blood

Bioanalysis ◽

10.4155/bio-2020-0271 ◽

2020 ◽

Vol 12 (24) ◽

pp. 1725-1737

Author(s):

Arundhuti Sen ◽

Molly Gillett ◽

Lucy Weaver ◽

Matthew Barfield ◽

Pawanbir Singh ◽

...

Keyword(s):

Human Blood ◽

Dried Blood Spots ◽

Extraction Process ◽

User Training ◽

Accuracy And Precision ◽

Volumetric Accuracy ◽

Performance Results ◽

The Impact ◽

And Storage

Background: The hemaPEN is a liquid microsampling device for the reproducible collection and storage of blood samples as dried blood spots, for subsequent quantitative analysis. Materials & methods: We examined the device’s ability to collect accurate and precise blood volumes, at different hematocrit levels, via in vitro studies using acetaminophen in human blood. We also investigated the impact of different user training approaches on device performance. Results: The hemaPEN demonstrated acceptable volumetric accuracy and precision, regardless of the training medium used. Issues with apparent hematocrit-dependent bias were found to be associated with the extraction process, rather than the volumetric performance of the device. Conclusion: The hemaPEN is capable of readily producing high quality blood microsamples for reproducible and accurate quantitative bioanalysis.

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Evaluation of the Impact of Excipients on Albendazole Concentrations in Upper Small Intestine Using an In Vitro Biorelevant Gastrointestinal Transfer (BioGIT) System

10.26226/morressier.57d6b2bbd462b8028d88e13f ◽

2016 ◽

Author(s):

Maria Vertzoni

Keyword(s):

Small Intestine ◽

The Impact

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The Predominant microRNAs in β-cell Clusters for Insulin Regulation and Diabetic Control

Current Drug Targets ◽

10.2174/1389450121666191230145848 ◽

2020 ◽

Vol 21 (7) ◽

pp. 722-734

Author(s):

Adele Soltani ◽

Arefeh Jafarian ◽

Abdolamir Allameh

Keyword(s):

Mirna Expression ◽

Expression Profiles ◽

Expression Patterns ◽

Diabetic Control ◽

In Vitro Proliferation ◽

Cell Functions ◽

Mirna Expression Profiles ◽

Multiple Processes ◽

The Impact

micro (mi)-RNAs are vital regulators of multiple processes including insulin signaling pathways and glucose metabolism. Pancreatic β-cells function is dependent on some miRNAs and their target mRNA, which together form a complex regulative network. Several miRNAs are known to be directly involved in β-cells functions such as insulin expression and secretion. These small RNAs may also play significant roles in the fate of β-cells such as proliferation, differentiation, survival and apoptosis. Among the miRNAs, miR-7, miR-9, miR-375, miR-130 and miR-124 are of particular interest due to being highly expressed in these cells. Under diabetic conditions, although no specific miRNA profile has been noticed, the expression of some miRNAs and their target mRNAs are altered by posttranscriptional mechanisms, exerting diverse signs in the pathobiology of various diabetic complications. The aim of this review article is to discuss miRNAs involved in the process of stem cells differentiation into β-cells, resulting in enhanced β-cell functions with respect to diabetic disorders. This paper will also look into the impact of miRNA expression patterns on in vitro proliferation and differentiation of β-cells. The efficacy of the computational genomics and biochemical analysis to link the changes in miRNA expression profiles of stem cell-derived β-cells to therapeutically relevant outputs will be discussed as well.

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The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180502120804 ◽

2018 ◽

Vol 16 (2) ◽

pp. 127-137

Author(s):

Paula Sofia Coutinho Medeiros ◽

Ana Lúcia Marques Batista de Carvalho ◽

Cristina Ruano ◽

Juan Carlos Otero ◽

Maria Paula Matos Marques

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cell Line ◽

Breast Cancer Cells ◽

Triple Negative ◽

Breast Adenocarcinoma ◽

Coumaric Acid ◽

Human Breast ◽

The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

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VDAC1 Mediated Anticancer Activity of Gallic Acid in Human Lung Adenocarcinoma A549 Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170912115441 ◽

2018 ◽

Vol 18 (2) ◽

pp. 255-262 ◽

Cited By ~ 5

Author(s):

Aikebaier Maimaiti ◽

Amier Aili ◽

Hureshitanmu Kuerban ◽

Xuejun Li

Keyword(s):

Western Blot ◽

Cell Viability ◽

Gallic Acid ◽

Molecular Mechanisms ◽

A549 Cells ◽

Dependent Manner ◽

Lung Carcinoma Cells ◽

Induced Apoptosis ◽

The Impact

Aims: Gallic acid (GA) is generally distributed in a variety of plants and foods, and possesses cell growth-inhibiting activities in cancer cell lines. In the present study, the impact of GA on cell viability, apoptosis induction and possible molecular mechanisms in cultured A549 lung carcinoma cells was investigated. Methods: In vitro experiments showed that treating A549 cells with various concentrations of GA inhibited cell viability and induced apoptosis in a dose-dependent manner. In order to understand the mechanism by which GA inhibits cell viability, comparative proteomic analysis was applied. The changed proteins were identified by Western blot and siRNA methods. Results: Two-dimensional electrophoresis revealed changes that occurred to the cells when treated with or without GA. Four up-regulated protein spots were clearly identified as malate dehydrogenase (MDH), voltagedependent, anion-selective channel protein 1(VDAC1), calreticulin (CRT) and brain acid soluble protein 1(BASP1). VDAC1 in A549 cells was reconfirmed by western blot. Transfection with VDAC1 siRNA significantly increased cell viability after the treatment of GA. Further investigation showed that GA down regulated PI3K/Akt signaling pathways. These data strongly suggest that up-regulation of VDAC1 by GA may play an important role in GA-induced, inhibitory effects on A549 cell viability.

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Effects of Transport Medium Composition on in vitro Drug Permeation across Excised Pig Intestinal Tissue

Drug Delivery Letters ◽

10.2174/2210303110999201005214114 ◽

2020 ◽

Vol 10 ◽

Author(s):

Bianca Peterson ◽

Henrico Heystek ◽

Josias H. Hamman ◽

Johan D. Steyn

Keyword(s):

Medium Composition ◽

Screening Tests ◽

Intestinal Tissue ◽

Predictive Values ◽

Transport Medium ◽

Intestinal Fluids ◽

Lower Permeability ◽

The Impact ◽

Simulated Intestinal Fluids

Background:: Knowledge of the permeation characteristics of new chemical entities across biological membranes is essential to drug research and development. Transport medium composition may affect the absorption of compounds during in vitro drug transport testing. To preserve the predictive values of screening tests, the possible influence of transport media on the solubility of model drugs, and on the activities of tight junctions and efflux transporter proteins (e.g. P-glycoprotein) must be known. Objective:: The aim of this study was to compare the impact of different transport media on the bi-directional transport of standard compounds, selected from the four classes of the Biopharmaceutical Classification System (BCS), across excised pig intestinal tissue. Methods:: The Sweetana-Grass diffusion apparatus was used for the transport studies. Krebs-Ringer bicarbonate (KRB) buffer and simulated intestinal fluids in the fed (FeSSIF) and fasted (FaSSIF) states were used as the three transport media, while the chosen compounds were abacavir (BCS class 1), dapsone (BCS class 2), lamivudine (BCS class 3) and furosemide (BCS class 4). Results:: Abacavir exhibited lower permeability in both the simulated intestinal fluids than in the KRB buffer. Dapsone showed similar permeability in all media. Lamivudine exhibited lower permeability in FaSSIF than in the other two media. Furosemide exhibited improved transport with pronounced efflux in FaSSIF. Conclusion:: Different permeation behaviors were observed for the selected drugs in the respective media, which may have resulted from their different physico-chemical properties, as well as from the effects that dissimilar transport media components had on excised pig intestinal tissue.

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