scholarly journals In vitro Evaluation of Actinobacteria against Tomato Bacterial Wilt (Ralstonia solanacearum EF Smith) in West Showa, Ethiopia

2012 ◽  
Vol 04 (01) ◽  
Author(s):  
Kassahun Sadessa Biratu
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
In Vitro Evaluation ◽  
Tomato Bacterial Wilt

scholarly journals Effect of Allium fistulosum Extract on Ralstonia solanacearum Populations and Tomato Bacterial Wilt

Plant Disease ◽  
2012 ◽  
Vol 96 (5) ◽  
pp. 687-692 ◽  
Author(s):  
Péninna Deberdt ◽  
Benjamin Perrin ◽  
Régine Coranson-Beaudu ◽  
Pierre-François Duyck ◽  
Emmanuel Wicker
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Management Strategies ◽  
Antimicrobial Effect ◽  
Allium Fistulosum ◽  
Inhibition Assay ◽  
In Vivo Experiments ◽  
Tomato Bacterial Wilt

To control bacterial wilt (Ralstonia solanacearum, phylotype IIB/4NPB), the antimicrobial effect of Allium fistulosum aqueous extract was assessed as a preplant soil treatment. Three concentrations of extract (100, 50, and 25%, 1:1 [wt/vol]) were evaluated by in vitro inhibition assay and in vivo experiments in a growth chamber. In vitro, A. fistulosum (100 and 50%) suppressed growth of R. solanacearum. Preplant treatment of the soil with A. fistulosum extract significantly reduced the R. solanacearum populations. No pathogen was detected in the soil after treatment with 100% concentrated extract from the third day after application until the end of the experiment. A. fistulosum also significantly reduced the incidence of tomato bacterial wilt. In the untreated control, the disease affected 61% of the plants whereas, with 100 and 50% extracts, only 6 and 14% of the plants, respectively, were affected. These results suggest that A. fistulosum extracts could be used in biocontrol-based management strategies for bacterial wilt of tomato.

Rhizophagus irregularis MUCL 41833 transitorily reduces tomato bacterial wilt incidence caused by Ralstonia solanacearum under in vitro conditions

Mycorrhiza ◽  
2017 ◽  
Vol 27 (7) ◽  
pp. 719-723 ◽  
Author(s):  
Marie Chave ◽  
Patrice Crozilhac ◽  
Péninna Deberdt ◽  
Katia Plouznikoff ◽  
Stéphane Declerck
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Rhizophagus Irregularis ◽  
Tomato Bacterial Wilt ◽  
Wilt Incidence

Effectiveness of Egyptian propolis on control of tomato bacterial wilt caused by Ralstonia solanacearum

2017 ◽  
Vol 124 (5) ◽  
pp. 467-472 ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Mohamed E. A. Seleim ◽  
Rafeek M. El-Sharkawy ◽  
Hadel M. M. Khalil Bagy
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Tomato Bacterial Wilt

scholarly journals Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

2018 ◽  
Vol 28 (1) ◽  
Author(s):  
Narasimhamurthy Konappa ◽  
Soumya Krishnamurthy ◽  
Chandra Nayaka Siddaiah ◽  
Niranjana Siddapura Ramachandrappa ◽  
Srinivas Chowdappa
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Trichoderma Asperellum ◽  
Biological Efficacy ◽  
Tomato Bacterial Wilt

scholarly journals Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms

2021 ◽  
Vol 12 ◽  
Author(s):  
Khanh Duy Le ◽  
Jeun Kim ◽  
Hoa Thi Nguyen ◽  
Nan Hee Yu ◽  
Ae Ran Park ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Foliar Application ◽  
Antagonistic Activity ◽  
Fungal Diseases ◽  
Dependent Manner ◽  
Minimum Concentration ◽  
Bacteria And Fungi ◽  
Tomato Bacterial Wilt

Plant bacterial and fungal diseases cause significant agricultural losses and need to be controlled. Beneficial bacteria are promising candidates for controlling these diseases. In this study, Streptomyces sp. JCK-6131 exhibited broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In vitro assays showed that the fermentation filtrate of JCK-6131 inhibited the growth of bacteria and fungi with minimum concentration inhibitory (MIC) values of 0.31–10% and 0.31–1.25%, respectively. In the in vivo experiments, treatment with JCK-6131 effectively suppressed the development of apple fire blight, tomato bacterial wilt, and cucumber Fusarium wilt in a dose-dependent manner. RP-HPLC and ESI-MS/MS analyses indicated that JCK-6131 can produce several antimicrobial compounds, three of which were identified as streptothricin E acid, streptothricin D, and 12-carbamoyl streptothricin D. In addition, the disease control efficacy of the foliar application of JCK-6131 against tomato bacterial wilt was similar to that of the soil drench application, indicating that JCK-6131 could enhance defense resistance in plants. Molecular studies on tomato plants showed that JCK-6131 treatment induced the expression of the pathogenesis-related (PR) genes PR1, PR3, PR5, and PR12, suggesting the simultaneous activation of the salicylate (SA) and jasmonate (JA) signaling pathways. The transcription levels of PR genes increased earlier and were higher in treated plants than in untreated plants following Ralstonia solanacearum infection. These results indicate that Streptomyces sp. JCK-6131 can effectively control various plant bacterial and fungal diseases via two distinct mechanisms of antibiosis and induced resistance.

scholarly journals Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro

2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biological Control Agent ◽  
Wilt Disease ◽  
Molecular Characteristics ◽  
Streptomyces Sp ◽  
Bacterial Wilt Disease ◽  
Wide Range

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.

Distribution and incidence of tomato bacterial wilt caused by Ralstonia solanacearum in the central region of Togo

2021 ◽  
Author(s):  
Bitang Bamazi ◽  
Agnassim Banito ◽  
K. D. Ayisah ◽  
Rachidatou Sikirou ◽  
Mathews Paret ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Laboratory Investigation ◽  
Central Region ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Yield Losses ◽  
Tomato Production ◽  
Solanum Lycopersicum L ◽  
Survey Results ◽  
Tomato Bacterial Wilt

Tomato (Solanum lycopersicum L.) is one of the most important vegetables in Togo. Unfortunately, tomatoes are susceptible to many diseases, among which bacterial wilt caused by Ralstonia solanacearum causes major yield losses. In this study, incidence of bacterial wilt and its distribution was evaluated in the central region of Togo, the major tomato producing area in the country. Overall, 16 localities were surveyed in four prefectures. In each locality, three fields were visited, and the incidence of the disease was recorded, and diseased samples were collected for laboratory investigation. The results showed that bacterial wilt occurred in all the fields visited, indicating a field incidence of 100%, whereas the plant incidence ranged from 10.00±00% to 43.33±3.33%, with an average of 20.94±1.77%. The antibody based Immunostrip test was positive for R. solanacearum in 100% of the visited fields. From 144 samples collected from fields, 45 R. solanacearum isolates were isolated on Modified SMSA media. This survey results show that tomato bacterial wilt is a real threat to tomato production in the central region of Togo.

scholarly journals Comparison of Bacterial Community Structures in the Rhizoplane of Tomato Plants Grown in Soils Suppressive and Conducive towards Bacterial Wilt

1999 ◽  
Vol 65 (9) ◽  
pp. 3996-4001 ◽  
Author(s):  
Yoshitaka Shiomi ◽  
Masaya Nishiyama ◽  
Tomoko Onizuka ◽  
Takuya Marumoto
Keyword(s):  
Bacterial Wilt ◽  
In Vitro Assays ◽  
Gram Negative Bacteria ◽  
16S Rdna Sequence ◽  
Tomato Plants ◽  
16S Rdna Sequence Analysis ◽  
Bacterial Groups ◽  
Chloroform Fumigation ◽  
Tomato Bacterial Wilt

ABSTRACT It has been reported that the growth of Ralstonia solanacearum is suppressed at the rhizoplane of tomato plants and that tomato bacterial wilt is suppressed in plants grown in a soil (Mutsumi) in Japan. To evaluate the biological factors contributing to the suppressiveness of the soil in three treated Mutsumi soils (chloroform fumigated soil; autoclaved soil mixed with intact Mutsumi soil; and autoclaved soil mixed with intact, wilt-conducive Yamadai soil) infested with R. solanacearum, we bioassayed soil samples for tomato bacterial wilt. Chloroform fumigation increased the extent of wilt disease. More of the tomato plant samples wilted when mixed with Yamadai soil than when mixed with Mutsumi soil. Consequently, the results indicate that the naturally existing population of microorganisms in Mutsumi soil was significantly able to reduce the severity of bacterial wilt of tomato plants. To characterize the types of bacteria present at the rhizoplane, we isolated rhizoplane bacteria and classified them into 22 groups by comparing their 16S restriction fragment length polymorphism patterns. In Yamadai soil a single group of bacteria was extremely predominant (73.1%), whereas in Mutsumi soil the distribution of the bacterial groups was much more even. The 16S rDNA sequence analysis of strains of dominant groups suggested that gram-negative bacteria close to the β-proteobacteria were most common at the rhizoplane of the tomato plants. During in vitro assays, rhizoplane bacteria in Mutsumi soil grew more vigorously on pectin, one of the main root exudates of tomato, compared with those in Yamadai soil. Our results imply that it is difficult for the pathogen to dominate in a diversified rhizobacterial community that thrives on pectin.

Sign in / Sign up

Export Citation Format

Share Document