scholarly journals Research Article Phosphate solubilizing activity of native soil microorganisms from the rhizosphere of Jatropha curcas and from phosphate-solubilizing bacteria inoculum

2019 ◽  
Vol 18 (4) ◽  
Author(s):  
E.B. Santana ◽  
J.C.T. Dias
Keyword(s):  
Soil Microorganisms ◽  
Phosphate Solubilizing Bacteria ◽  
Research Article ◽  
Native Soil ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

scholarly journals Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Meli Astriani ◽  
SITI ZUBAIDAH ◽  
ABDUL LATIEF ABADI ◽  
ENDANG SUARSINI
Keyword(s):  
Acetic Acid ◽  
Molecular Identification ◽  
Phosphate Solubilizing Bacteria ◽  
Chemical Fertilizers ◽  
Iaa Production ◽  
Novel Bacterium ◽  
Pseudomonas Plecoglossicida ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity ◽  
Indole 3 Acetic Acid

Abstract. Astriani M, Zubaidah S, Abadi AL, Suarsini E. 2020. Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia. Biodiversitas 21: 578-586. The use of synthetic fertilizers to grow soybean (Glycine max (L.) Merrill) in a long time, can increase the risk of environmental damage. Therefore, the current study aimed to find phosphate solubilizing bacteria that can produce indole-3-acetic acid (IAA) hormone to minimize the use of chemical fertilizers. Superior isolates selected through characterization of phosphate solubilization activity on Pikovskaya medium, screening of IAA producing bacteria, quantitative estimation of phosphate solubilizing activity using a spectrophotometer, hypersensitivity assay, antagonist within isolate assay, and molecular identification of selected bacterial isolates using 16S rRNA sequencing with primer forward 63f and primer reverse 1387r. Among the isolated bacteria, isolate Arj8 showed the highest phosphate solubilizing activity and IAA production. Molecular identification indicated that isolate Arj8 shared 100% similarity with Pseudomonas plecoglossicida. The highest phosphate solubilizing activity (75.39 mg/L) and IAA production (38.89 ppm) recorded on day-3. Multiple potentialities of P. plecoglossicida as phosphate solubilizing and IAA producing bacterium are a novel finding in the development of bioinoculants as bio-fertilizers that can reduce dependency on synthetic chemical fertilizers.

scholarly journals Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere

2016 ◽  
Vol 8 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Anurag Yadav ◽  
Kusum Yadav ◽  
Anupam Vashistha
Keyword(s):  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Ph Value ◽  
Culture Collection ◽  
Phosphate Solubilizing Bacteria ◽  
Linear Regression Equation ◽  
Wheat Rhizosphere ◽  
Bacterial Treatment ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.

The Presence of Beneficial Organisms Associated to N and P Economy in the Rhizosphere of Native Vegetation in an Oligotrophic Savanna of Guárico State, Venezuela

2017 ◽  
Vol 10 (1) ◽  
pp. 123-133
Author(s):  
Edith Mora ◽  
Marcia Toro ◽  
Danilo López-Hernández
Keyword(s):  
Soil Microorganisms ◽  
P Uptake ◽  
Ecological Niches ◽  
Phosphate Solubilizing Bacteria ◽  
Natural Conditions ◽  
Tropical Plants ◽  
Plant P Uptake ◽  
Am Symbiosis ◽  
Phosphate Solubilizing ◽  
Total Bacteria

Background:In natural conditions, tropical plants are adapted to different ecological niches that can be associated to soil microorganisms which play a key role in nutrient cycling likeArbuscular mycorrhiza(AM), phosphate solubilizing bacteria (PSB) and/or nitrogen fixing rhizobia.Methods:We report a survey of the presence in a Trachypogon savanna located at Estación Experimental La Iguana (EELI) in Central Venezuela, of some beneficial plant-microorganism associations. In this savanna, plants present a high AM symbiosis affinity. The high mycorrhization and the presence of potential PSB suggest a synergic effect in plant P-uptake.Results:After screening the rhizospheres of 25 plant species from the zone, we could isolate a high proportion of potential PSB in relation to the total bacteria number from the rhizospheres ofCentrosema venosumandGalactia jussiaeana.Conclusion:Therefore, the presence of potential PSB in the rhizosphere of those species constitutes an important finding to discover novel biofertilizers for crop plants.

PHOSPHATSOLUBILIZING BACTERIA OF THE GENUS PSEUDOMONAS AND THE EFFICIENCY OF THEIR APPLICATION TO INCREASE THE AVAILABILITY OF PHOSPHORUS

2021 ◽  
Vol 0 (3) ◽  
pp. 11-16
Author(s):  
E.V. KUZINA ◽  
◽  
G.F. RAFIKOVA ◽  
T.YU. KORSHUNOVA ◽  
◽  
...  
Keyword(s):  
Nitrogen Fertilizers ◽  
Phosphate Solubilizing Bacteria ◽  
Mobile Phosphorus ◽  
Insoluble Phosphate ◽  
Treatment Facilities ◽  
Agar Media ◽  
Phosphate Solubilizing ◽  
Solid Nutrient Medium ◽  
Solubilizing Activity

Phosphorus is the second most important element for plants after nitrogen. Fertilizers based on it, used to stimulate productivity, are inaccessible for most crops, which leads to their accumulation in the soil and environmental pollution. The use of phosphate-solubilizing bacteria increases the amount of phosphorus absorbed by plants. In most publications describing this group of bacteria, their effectiveness is assessed only in vitro by the halo zones formed on agar media with calcium orthophosphate. The aim of this study was to compare the solubilizing properties of bacteria of the genus Pseudomonas on a solid nutrient medium, as well as in sand and soil. It was shown that all studied cultures of microorganisms are capable of solubilizing insoluble phosphate in Pikovskaya's medium. The most active strains were Pseudomonas laurentiana ANT 56 and Pseudomonas sp. IB 182, isolated from the activated sludge of biological treatment facilities and arable soil, respectively. Experiments with the introduction of strains showed that the amount of mobile phosphorus in the sand increased 2.6-3.8 times in two weeks (in the control 1.2 times), while in the experiment with soil, a significant increase in the content of mobile phosphorus compared to the control was recorded only for the strain P . laurentiana ANT 17 (by 29.1%). It is assumed that the high solubilizing activity of the P. laurentiana ANT 17 strain may be due to the complex action of mechanisms of different nature, including the synthesis of indolyl-3-acetic acid and exopolysaccharide. The studies carried out make it possible to consider this bacterial strain as a promising object for creating on its basis a biological preparation for agricultural purposes.

scholarly journals BIODIVERSITY OF PHOSPHATE SOLUBILIZING FUNGIISOLATED FROM AGRICULTURAL FIELDS OF MARATHWADA REGION

2021 ◽  
Vol 9 (07) ◽  
pp. 799-804
Author(s):  
Parwez Qayum ◽  
◽  
J.D. Shaikh ◽  
Keyword(s):  
Soil Microorganisms ◽  
Soil Depth ◽  
Crop Productivity ◽  
Fungal Species ◽  
Trichoderma Spp ◽  
Fungal Isolates ◽  
Core Components ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity ◽  
Penicillium Spp

Fungi are the core components of soil microorganisms, accounting for more of the soil biomass than bacteria, depending on soil depth and nutritional requirements. In the current study, phosphate solubilizing fungi were isolated from the soil of Maharashtras Marathwada area, and all fungal isolates were examined for their ability to solubilize phosphate.Only 11 fungal isolates out of a total of 40 were found to have P-solubilizing activity. After 48 hours of incubation, the fungal isolates Aspergillus niger (PQ9), Trichoderma spp (PQ36), and Penicillium spp (PQ19) demonstrated a considerable zone of solubilization with 34, 31 to 30 mm on selective agar medium. The potent phosphate solubilizing fungi were identified in 18SrRNA analysis.The study, therefore, proposed that these fungal species have strong phosphate solubilizing properties and can be used for excellent crop productivity as a biofertilizer.

scholarly journals USE OF 16S rRNA GENE FOR CHARACTERIZATION OF PHOSPHATE-SOLUBILIZING BACTERIA ASSOCIATED WITH CORN

2009 ◽  
Vol 32 (1) ◽  
pp. 31-37
Author(s):  
David Espinosa-Victoria ◽  
Lucía López-Reyes ◽  
Aldo De La Cruz-Benítez
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
Accession Number ◽  
Ncbi Accession ◽  
Buffering Agent ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity ◽  
Ncbi Accession Number

Thirty-six strains of phosphate-solubilizing bacteria (PSB) isolated from the rhizosphere and rhizoplane of corn (Zea mays L.) crops in different states of México were subjected to phenotypic and genotypic characterization. The phosphate-solubilizing activity of each strain was first evaluated using tricalcium phosphate as the phosphorus source in the NBRIP-BPB culture medium. Phosphatesolubilizing capacity was also evaluated by adding the pH buffering agent MES (2-[Morpholine] ethanosulfonic acid) to the growth medium. Amplified ribosomal RNA restriction pattern analysis (ARDRA) was used to evaluate the genetic diversity. From the data matrix obtained, a dendrogram was built using the UPGMA method. The 16S rRNA gene of the BUAP29, BUAP36 and CP08 strains was amplified, cloned and sequenced for taxonomic identification. The 36 bacterial strains exhibited different levels of tricalcium phosphate solubilizing activity. Only BUAP33, BUAP17 and BUAP21 strains did not show the typical solubilization halo when the MES buffering agent was added to the growth medium. The analysis of ARDRA patterns as well as the dendrogram exhibited a large genetic diversity among the 36 PSB analyzed, with BUAP36 and BUAP15 strains showing 100 % similarity. The 16S rRNA gene sequence alignment of CP08, BUAP29 and BUAP36 strains showed 99 % identity with the sequences of Advenella incenata strain R-16599 (NCBI accession number AY569458.1), Burkholderia sp (NCBI accession number AY353696) and Burkholderia gladioli strain 223-1 (NCBI accession number DQ355168.1), respectively. In this study the A. incenata strain is reported as a PSB for the first time.

scholarly journals Manajemen Pemupukan N-P-K dengan Pupuk Hayati untuk Mempertahankan Kandungan N dan P Tanah serta Hasil Kedelai

Jurnal Agro ◽  
10.15575/1201 ◽  
2017 ◽  
Vol 4 (1) ◽  
pp. 15-23
Author(s):  
Emma Trinurani Sofyan ◽  
Stefina Liana Sari
Keyword(s):  
Soil Microorganisms ◽  
Block Design ◽  
Inorganic Fertilizer ◽  
Soybean Plant ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Nitrogen Fixing Bacteria ◽  
Available Nutrients ◽  
Randomized Block Design ◽  
Phosphate Solubilizing

One effort to increase the productivity of soybeans in Inceptisols Jatinangor is through utilize inoculant of soil microorganisms such as nitrogen-fixing bacteria and phosphate-solubilizing bacteria in the form of specific biofertilizer (SB) for soybean plant without leaving inorganic fertilizer as a source of available nutrients to plants. This research aimed to determine fertilization management of N-P-K and specific biofertilizer in retaining N and P availability and yield of soybean. The Research was conducted from April to July 2016, arranged in Simple Randomized Block Design (RBD) and consisted of 10 treatments. The combination of biofertilizer and N-P-K were as follows; A = control (without fertilizer), B = Standard N-P-K, C = 0 N-P-K + 1 SB , D = 1/4 N-P-K + 1 SB, E = 1/2 N-P-K + 1 SB, F = 3/4 N-P-K + 1 SB, G = 1 N-P-K + 1 SB, H = 3/4 + 1/4 SB, I = 3/4 N-P-K + 1/2 SB dan J = 3/4 N-P-K + 3/4 SB. The results showed a dose of N-P-K and biofertilizer was recommended in the treatment E (250 kg ha-1 SB) and 1/2 N-P-K (37.5-50-50 kg ha-1). The dose was able to increase nitrogen and phosphor content in soil and plant, also on yield of soybean up to 0.4 t ha-1 or 32% of a standard N-P-K.

Isolation and Identification of Potential Phosphate Solubilizing Bacteria from the Rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh

2007 ◽  
Vol 62 (1-2) ◽  
pp. 103-110 ◽  
Author(s):  
Md. Tofazzal Islam ◽  
Abhinandan Deora ◽  
Yasuyuki Hashidoko ◽  
Atiqur Rahman ◽  
Toshiaki Ito ◽  
...  
Keyword(s):  
Ph Value ◽  
Oryza Sativa L ◽  
Agar Plate ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Acinetobacter Sp ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

A total of 30 bacteria were isolated from the rhizoplane of rice cv. BR29 cultivated in Mymensingh, Bangladesh and from the seedlings obtained from surface-sterilized seeds of BR29. Upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using National Botanical Research Institute’s phosphate medium. The bacterial isolates were identified based on their phenotypic and 16S rRNA genes sequencing data as Acinetobacter sp. BR-12, Klebsiella sp. BR-15, Acinetobacter sp. BR-25, Enterobacter sp. BR-26, Microbacterium sp. BRS-1 and Pseudomonas sp. BRS-2. The BR-25 exhibited highest phosphate solubilizing activity followed by BR-15. They grew rapidly in the liquid medium at pH 5 and 7 but almost no growth occurred at pH 3. The pH value of the culture medium was decreased with bacterial growth suggesting that they might secrete organic acids to solubilize insoluble phosphorus. Scanning electron microscope analysis of two-week-old rice seedlings germinated from seeds previously inoculated with BR-25 and BR-15 revealed dense colonization at the root surfaces presumably using fimbriae on the bacterial cells.

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