scholarly journals Molecular Examination of Trichomonas vaginalis Infection and Risk of Prostate Cancer in the Biopsy of Patients with Different Prostate Lesions

2021 ◽  
Vol 31 (2) ◽  
Author(s):  
Zeinab Kamarkhani ◽  
Raheleh Rafiei-Sefiddashti ◽  
Leila Haghighi ◽  
Alireza Badirzadeh
Keyword(s):  
Prostate Cancer ◽  
Trichomonas Vaginalis ◽  
Potential Role ◽  
The Elderly ◽  
Diagnostic Techniques ◽  
Causative Role ◽  
Sexually Transmitted ◽  
Polymerase Chain ◽  
Urological Diseases

BACKGROUND፡ Trichomoniasis is a sexually transmitted infectious disease caused by a flagellated protozoa, Trichomonas vaginalis (T.vaginalis) and is often asymptomatic in men. Benign prostatic hyperplasia (BPH) and prostate cancer (PCA) are the most common urological diseases in the elderly. Scientists have proposed various factors which trigger prostate cancer, including sexually transmitted diseases. Thus, this study aimed to evaluate the potential role of T. vaginalis as a risk factor for various prostate lesions such as hyperplasia and prostate cancer.METHODS: A total of 250 paraffin-embedded of different prostate lesion biopsies were analyzed by Polymerase Chain Reaction (PCR) using the beta-tubulin gene for identifying T. vaginalis.RESULT: All 250 pathologic specimens were negative for this parasite by using PCR technique.CONCLUSION: It seems that T. vaginalis may have not had a causative role for different prostate lesions and it seems proposed PCR technique is an insufficient method to find the parasite in paraffin-embedded tissues. Therefore, other diagnostic techniques to identify the parasite in biopsy samples are suggested.

scholarly journals Chest CT texture-based radiomics analysis in differentiating COVID-19 from other interstitial pneumonia

2021 ◽  
Author(s):  
Damiano Caruso ◽  
Francesco Pucciarelli ◽  
Marta Zerunian ◽  
Balaji Ganeshan ◽  
Domenico De Santis ◽  
...  
Keyword(s):  
Interstitial Pneumonia ◽  
Potential Role ◽  
Texture Features ◽  
Roc Curves ◽  
Chest Ct ◽  
Preliminary Evaluation ◽  
Rt Pcr ◽  
Mean Intensity ◽  
Polymerase Chain

Abstract Purpose To evaluate the potential role of texture-based radiomics analysis in differentiating Coronavirus Disease-19 (COVID-19) pneumonia from pneumonia of other etiology on Chest CT. Materials and methods One hundred and twenty consecutive patients admitted to Emergency Department, from March 8, 2020, to April 25, 2020, with suspicious of COVID-19 that underwent Chest CT, were retrospectively analyzed. All patients presented CT findings indicative for interstitial pneumonia. Sixty patients with positive COVID-19 real-time reverse transcription polymerase chain reaction (RT-PCR) and 60 patients with negative COVID-19 RT-PCR were enrolled. CT texture analysis (CTTA) was manually performed using dedicated software by two radiologists in consensus and textural features on filtered and unfiltered images were extracted as follows: mean intensity, standard deviation (SD), entropy, mean of positive pixels (MPP), skewness, and kurtosis. Nonparametric Mann–Whitney test assessed CTTA ability to differentiate positive from negative COVID-19 patients. Diagnostic criteria were obtained from receiver operating characteristic (ROC) curves. Results Unfiltered CTTA showed lower values of mean intensity, MPP, and kurtosis in COVID-19 positive patients compared to negative patients (p = 0.041, 0.004, and 0.002, respectively). On filtered images, fine and medium texture scales were significant differentiators; fine texture scale being most significant where COVID-19 positive patients had lower SD (p = 0.004) and MPP (p = 0.004) compared to COVID-19 negative patients. A combination of the significant texture features could identify the patients with positive COVID-19 from negative COVID-19 with a sensitivity of 60% and specificity of 80% (p = 0.001). Conclusions Preliminary evaluation suggests potential role of CTTA in distinguishing COVID-19 pneumonia from other interstitial pneumonia on Chest CT.

scholarly journals The Role of MicroRNAs in Selected Forms of Glomerulonephritis

2019 ◽  
Vol 20 (20) ◽  
pp. 5050 ◽  
Author(s):  
Magdalena Nalewajska ◽  
Klaudia Gurazda ◽  
Ewa Styczyńska-Kowalska ◽  
Małgorzata Marchelek-Myśliwiec ◽  
Andrzej Pawlik ◽  
...  
Keyword(s):  
Risk Factors ◽  
Immune System ◽  
Genetic Risk ◽  
Potential Role ◽  
Kidney Diseases ◽  
Diagnostic Techniques ◽  
Targeted Treatments ◽  
Harmful Effects ◽  
Insight Into

Glomerulonephritis (GN) represents a collection of kidney diseases characterized by inflammation within the renal glomeruli and small blood vessels. The lesions that occur in other nephron structures mainly result from the harmful effects of proteinuria. In recent years, an emphasis has been placed on gaining a better insight into the pathogenesis and pathophysiology of GN in order to facilitate diagnoses and provide efficient and targeted treatments of the disease. Owing to the advanced molecular and genetic diagnostic techniques available today, researchers have been able to elucidate that most cases of GN are determined by genetic risk factors and are associated with the abnormal functioning of the immune system (the immunologically mediated forms of GN). MicroRNAs (miRNAs) are a group of single-stranded, non-coding molecules, approximately 20 nucleotides in length, that act as regulatory factors in the post-transcriptional processes capable of regulating the expression of multiple genes. In this paper we present the available research aiming to determine effects of miRNAs on the development and progression of GN and discuss the potential role of miRNAs as new diagnostic markers and therapeutic targets.

scholarly journals Unexplained Anemia in the Elderly: Potential Role of Arterial Stiffness

2016 ◽  
Vol 7 ◽  
Author(s):  
David Montero ◽  
Candela Diaz-Cañestro ◽  
Andreas Flammer ◽  
Carsten Lundby
Keyword(s):  
Arterial Stiffness ◽  
Potential Role ◽  
The Elderly

Phosphoproteome analysis demonstrates the potential role of THRAP3 phosphorylation in androgen-independent prostate cancer cell growth

PROTEOMICS ◽  
2016 ◽  
Vol 16 (7) ◽  
pp. 1069-1078 ◽  
Author(s):  
Yoko Ino ◽  
Noriaki Arakawa ◽  
Hitoshi Ishiguro ◽  
Hiroji Uemura ◽  
Yoshinobu Kubota ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Potential Role ◽  
Prostate Cancer Cell ◽  
Cancer Cell Growth ◽  
Androgen Independent

Potential Role of Hyaluronic Acid on Bone in Osteoarthritis: Matrix Metalloproteinases, Aggrecanases, and RANKL Expression are Partially Prevented by Hyaluronic Acid in Interleukin 1-stimulated Osteoblasts

2014 ◽  
Vol 41 (5) ◽  
pp. 945-954 ◽  
Author(s):  
Zvezdana Mladenovic ◽  
Anne-Sophie Saurel ◽  
Francis Berenbaum ◽  
Claire Jacques
Keyword(s):  
Hyaluronic Acid ◽  
Matrix Metalloproteinases ◽  
Potential Role ◽  
Proteolytic Enzymes ◽  
Interleukin 1 ◽  
Nuclear Factor Κb ◽  
Mrna Levels ◽  
Receptor Activator ◽  
Polymerase Chain

Objective.To determine the effect of hyaluronic acid (HA) on proteolytic enzymes and bone remodeling mediators induced by interleukin 1β (IL-1β) and related to cartilage catabolism in murine osteoblasts.Methods.Osteoblasts were obtained from Swiss mice and cultured for 3 weeks. HA-treated osteoblasts were incubated with 100 μg/ml HA during the last week of culture, then stimulated with IL-1β (10 ng/ml) for 24 h. The expression of matrix metalloproteinases 3 and 13 (MMP-3 and MMP-13), ADAMTS-4 and ADAMTS-5, tissue inhibitor of metalloproteinases (TIMP), osteoprotegerin, and receptor activator of nuclear factor-κB ligand (RANKL) was determined by real-time polymerase chain reaction. MMP-3 and MMP-13 release was assessed by Western blot analysis.Results.IL-1β increased the mRNA levels of MMP-3 and MMP-13 and ADAMTS-4 and ADAMTS-5 and release of MMP-3 and MMP-13. Seven days of HA treatment significantly prevented the IL-1β-increased mRNA levels of MMP-3 (−61%, p < 0.01), MMP-13 (−56%, p < 0.01), ADAMTS-4 (−58%, p < 0.05), ADAMTS-5 (−52%, p < 0.01), and RANKL (−49%, p < 0.05), but not TIMP. As well, IL-1β-induced production of MMP-3 and MMP-13 was inhibited, by 27% (p < 0.01) and 40% (p < 0.01), respectively.Conclusion.In an inflammatory context in murine osteoblasts, HA can inhibit the expression of MMP and ADAMTS. Because HA can counteract the production of these mediators in chondrocytes, its beneficial effect in osteoarthritis may be due to its action on cartilage and subchondral bone.

scholarly journals Potential role of the OPG/RANK/RANKL axis in prostate cancer invasion and bone metastasis

2014 ◽  
Vol 32 (6) ◽  
pp. 2605-2611 ◽  
Author(s):  
XIAOMING LI ◽  
YAOMING LIU ◽  
BIN WU ◽  
ZHILONG DONG ◽  
YICHEN WANG ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Potential Role ◽  
Cancer Invasion
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