scholarly journals Chemical and microbial properties of kiln-smoked catfish in selected locations in Ilorin Metropolis, Nigeria

2021 ◽  
Vol 23 (2) ◽  
pp. 83-94
Author(s):  
O. A. Abiodun ◽  
A. Ojo ◽  
R. M. O. Kayode ◽  
V. E. Edem ◽  
M. O. Shittu ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Mineral Elements ◽  
Polycyclic Hydrocarbon ◽  
Salmonella Spp ◽  
Crude Fibre ◽  
Trichoderma Spp ◽  
Lead And Cadmium ◽  
Shigella Spp ◽  
Klebsiella Spp ◽  
Vibrio Spp

The use of smoking kiln for drying catfish is on the increase in Nigeria due to its effectiveness in reducing moisture and contamination with toxic compounds during smoking. Chemical properties of kiln-smoked catfish in selected locations in Ilorin metropolis were determined in this study. Kiln-smoked catfish were obtained from four major locations (Oyun, Asa-Dam, Tanke and Agbo-Oba) in Ilorin, Nigeria. The samples were subjected to chemical and polycyclic hydrocarbon content analyses. Dried catfish from all the locations had low moisture and crude fibre contents. There were no significant differences (p>0.05) in the crude fibre contents of the kiln-smoked catfish. Protein contents ranging from 53.72-56.49% were obtained for the kilnsmoked catfish samples. Fat contents ranged from 9.92-11.77% while the carbohydrate contents ranged from 19.02-23.59%. Potassium was the most abundant mineral in the samples with no traces of lead and cadmium. Agbo-Oba samples contained the highest pyrene (2983.215 ng/ml) and total polycyclic hydrocarbon (PAHs) (3349.090 ng/ml). Data on steroid fractions indicated that 17b-estradiol was significantly greater in quantity than those recorded from other steroid fractions. Microbial analyses of the samples revealed the presence of the following pathogenic organisms: Klebsiella spp, Salmonella spp, Shigella spp, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Vibrio spp, Aspergillus niger, penicilium spp, Trichoderma spp which are harmful to man. The use of smoking kiln method improved the nutrients and reduced PAHs in catfish. Keywords: Smoking kiln, Catfish, Chemical properties, Polycyclic hydrocarbon, Mineral elements

scholarly journals Assessment of microbiological quality of municipal supplied water within Dhaka Metropolis

2015 ◽  
Vol 4 (1) ◽  
pp. 28-30
Author(s):  
Rabeya Islam ◽  
Ahmad Kamruzzaman Majumder ◽  
Md Monzer Hossain Sarker
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Health Risks ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Pump Stations ◽  
Klebsiella Spp ◽  
Vibrio Spp

Microbiological health risks related to water quality are persisting issues especially in the developing countries. Present study was conducted to evaluate the microbiological quality of municipal supply water of a defined region within Dhaka metropolis, Bangladesh. A total of 20 water samples were collected from 10 selected pump stations and 10 different consuming points to examine the burden of Salmonella spp., Shigella spp., Citrobactor spp., Escherichia coli, Klebsiella spp., Aeromonas spp. and Vibrio spp. The study also included the evaluation of impacts due to the consumption of municipal water. The results revealed that the quality of municipal supplied water was not microbiologically safe for the consumers and also demonstrated the possible commencement of various diseases including diarrhoea, typhoid, dysentery and others. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22758 Stamford Journal of Microbiology, Vol.4(1) 2014: 28-30

scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals Microbiological analysis and antibacterial activity of pear samples

2016 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Habiba Afroz ◽  
Tasnia Ahmed ◽  
Md Aftab Uddin
Keyword(s):  
Fungal Growth ◽  
Bacterial Count ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Two Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Vibrio Spp

Present study attempted to isolate and quantify the microorganisms from the pear samples collected within Dhaka metropolis. Out of the five samples studied, total viable bacterial count and the total coliform count were near about 105 cfu/g and 104 cfu/g, respectively. Among the specific pathogens, prevalence of Salmonella spp. and Vibrio spp. were found only in two samples. Whereas Klebsiella spp, Pseudomonas spp. and Staphylococcus aureus were detected in all the samples studied. Fungal growth was also observed in each sample within the range of 8.8×104 – 9.5×104 cfu/g. The study of antibiogram revealed that, most of the pathogens were resistant to more than one antibiotic. Interestingly the pear samples showed no anti-bacterial activity against any of the laboratory isolates such as, Escherichia coli, Pseudomonas spp., Salmonella spp. and Bacillus spp.Stamford Journal of Microbiology, Vol.5(1) 2015: 1-4

scholarly journals Assessment of Antibiotics and Fluoride Toothpastes against Pathogenic Bacteria associated with Dental Caries

2018 ◽  
pp. 1-10
Keyword(s):  
Dental Caries ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Significant Activity ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Test Organisms ◽  
Susceptibility Tests ◽  
Vibrio Spp

Dental caries is the most contagious disease throughout the world. In the present study, a total of 100 dental caries patients were selected and samples from these patients were collected through sterile pincers for microbiological examination. A total of 9 different pathogenic bacterial isolates were identified through conventional culturing technique and among them maximum number of occurrence was by Salmonella spp (26.13%), followed by E. coli (17.39%), Proteus spp (17.39%), Shigella spp (8.69%), Pseudomonas spp (8.69%), Vibrio spp (8.69%), Enterobacter spp (4.34%), Yersinia spp (4.34%) and S. mutans (4.34%). Furthermore, antibiotic susceptibility tests were performed by 9 different commercially available antibiotics i.e. vancomycin, erythromycin, clindamycin, amoxicillin, tetracycline, rifampicin, ampicillin, ticarcillin and metronidazole/silver sulphadizine. Kirby Bauer disc diffusion method was used against test organisms and it was observed that all these antibiotics expressed significant activity against greater part of test organisms but few of them showed resistance to metronidazole/silver sulphadizine. In addition to this, different toothpastes encoded as TP-1, TP-2, TP-3, TP-4 and TP-5 were used at a concentration of 20 mg/mL in order to evaluate their activity against test organisms and it was observed that all these toothpastes showed significant activity against isolated bacteria. It was concluded that all these toothpastes contained fluoride which supports to avoid tooth decay and promote minty fresh breath. Furthermore, it was recommended that avoid usage of too much sweets, chocolates, candies especially in children/adults, and there must be a proper use of toothpastes twice a day at every stage of life. Keywords: Dental Caries, Pathogenic Bacteria, Antibiotics, Fluoride Toothpastes

scholarly journals Detection of Vibrio spp., Salmonella spp., and Shigella spp. among the frozen food samples employing enrichment culture technique

2016 ◽  
Vol 5 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Tahmina Shammi
Keyword(s):  
Enrichment Culture ◽  
Bacterial Load ◽  
False Negative ◽  
Food Samples ◽  
Food Preservation ◽  
Salmonella Spp ◽  
Potential Health ◽  
Frozen Food ◽  
Shigella Spp ◽  
Vibrio Spp

Freezing has long been an established method for food preservation. Freezing temperature may act as a stress factor for microbial cells, transforming the cells into injured or dormant state. Upon inoculation, these debilitated cells cannot grow on solid media and hence produce false negative results. Foods contaminated with injured cells of pathogenic bacterial strains are of potential health risk. Employing enrichment cultivation technique, present study attempted to detect such injured, dormant or viable but non culturable (VBNC) cells in different frozen food samples, collected from local markets and super-shops of Dhaka metropolis. Compared to the conventional cultivation means, the enrichment procedure revealed a significant increase in bacterial burden as well as increase in the pathogenic load. A maximum of 3 log increase in case of total bacterial load while 4 log, 5 log and 2 log increase in case of Vibrio spp., Salmonella spp. and Shigella spp., consecutively were observed. These findings clearly demonstrated the presence of injured cells in frozen foods which could be lethal under normal condition thereby posing public health risk.Stamford Journal of Microbiology, Vol.5(1) 2015: 26-29

scholarly journals Identification and antibiogram study of bacteria isolated from different street food

2018 ◽  
Vol 4 (3) ◽  
pp. 279-287
Author(s):  
Mohamed Ahmed Khalif ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Aoulad Hosen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Wide Spectrum ◽  
Salmonella Spp ◽  
Street Vendor ◽  
Street Food ◽  
Food Borne ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Food borne diseases are an increasingly recognized problem involving a wide spectrum of illnesses caused by bacterial contamination of food.Microorganism poses potential human health problems and is mainly transmitted through consumption of contaminated foods .Bangladesh is one of the densely populated country where majority of the people consume cheap foods prepared in unlicensed food selling points. The objective of the present study was to investigate the microbiological quality of different street food (Amra, Fusca, Chanachur and Guava) sold by various street vendor at Dinajpur, Bangladesh. A total of 20 samples were collected randomly from street vendors and tested for the presence of bacteria following standard microbiological method used for isolation, enumaration and identification of bacreria. Among the samples all had bacterial contamination. The total viable count (TVC) in diferrent street food samples was ranged from 8.0 × 105 CFU/g to 6.7x107 CFU/g. Among samples Escherichia coli 7(35%), Staphylococcus spp. 6(30%), Klebsiella spp. 5(25%), Salmonella spp. 1(5%) and Shigella spp. 1 (5%) were isolated. Escherichia coli were found highest 3(50%) Amra samples and 2(40%) Guava samples, then another organisms Staphylococcus spp. was found highest 2(50%) Chanachur and 2(40%) Fusca samples and then Klebsiella spp. was found highest 2(40%) Fusca samples. Antibiotic sensitivity test showed that Shigella spp., Staphylococcus spp., Klebsiella spp., Salmonella spp. and Escherichia coli were sensitive to Ciprofloxacin and Salmonella spp. were resistance to Gentamycin. All isolates found resistant to Cefixime, Cefalexin, Erythromycin, Fusidic acid, Cefuroxime and Aztreonam. All of the sample harbor multidrug resistant food borne bacteria which might cause public health hazards if these antibiotic resistance transfer to human.Asian J. Med. Biol. Res. September 2018, 4(3): 279-287

scholarly journals Microbiological quality assessment for drug resistant pathogenic microorganisms from the fresh vended fruit juices

2017 ◽  
Vol 6 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Nurunnahar Akter Lucky ◽  
Ifra Tun Nur ◽  
Tasnia Ahmed
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Fruit Juices ◽  
Drug Resistant ◽  
Pathogenic Microorganisms ◽  
Salmonella Spp ◽  
Juice Processing ◽  
Nutritive Values ◽  
Shigella Spp ◽  
Vibrio Spp

Fruit juices are very popular due to their freshness and taste. These juices contain all the nutritive values those were present in whole fruits. Fresh juices are healthful drink for people of all ages. But the condition is quite opposite if they become contaminated with pathogenic bacteria during the juice processing. Because of the nutritive properties juice can give enough opportunities to the microorganisms to survive and multiply. In our current study we included eight types of fresh juices to study the microbial load. All the samples were heavily contaminated. Total bacterial and fungal count was found to be up to 3.0×107 cfu/ml and 2.6×106 cfu/ml, respectively. Salmonella spp. and Shigella spp. was present only in one sample. Staphylococcus aureus and Pseudomonas spp. were the most prominent in all of the eight samples. Vibrio spp. was found in six samples ranging from 2.0×102 cfu/ml to 2.9×104 cfu/ml. All the pathogenic isolates showed drug resistance towards the most commonly prescribed antibiotics indicating the risk of difficulty in eradicating diseases.Stamford Journal of Microbiology, Vol.6(1) 2016: 7-10

scholarly journals Prevalence of Pathogenic Bacteria in Shrimp Samples Collected from Hatchery, Local Markets and the Shrimp Processing Plant

2016 ◽  
Vol 29 (1) ◽  
pp. 7-10 ◽  
Author(s):  
M Majibur Rahman ◽  
Farjana Rahman ◽  
Farzana Afroze ◽  
Farzana Yesmin ◽  
Kazi Kaniz Fatema ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Economic Interests ◽  
Local Markets ◽  
E Coli ◽  
Export Quality ◽  
Klebsiella Spp ◽  
Vibrio Spp

Export quality frozen shrimps comprise as one of the major economic interests in Bangladesh. During cultivation or processing and packaging, microbial condition is an important issue to maintain the quality of shrimps. Eight shrimp samples, of which, one from hatchery, 3 from local markets and 4 processed export quality samples were studied for microbiological risk assessment (MRA). One hatchery, three market and two export quality shrimp samples were found to contain bacterial pathogens probationary identified as Escherichia coli, Klebsiella spp., Vibrio spp., Aeromonas spp., Pseudomonas spp., Listeria spp., Shigella-like organisms, Staphylococcus aureus, and Salmonella spp. and two other processed export quality shrimp samples were completely free of pathogens of any type. Surprisingly, the later two processed export quality shrimp samples showed antibacterial activity against E. coli, Klebsiella sp. and Vibrio sp.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 7-10

scholarly journals CTX-M extended-spectrum β-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals

2016 ◽  
Vol 47 (3) ◽  
pp. 706-711 ◽  
Author(s):  
Abed Zahedi Bialvaei ◽  
Hossein Samadi Kafil ◽  
Mohammad Asgharzadeh ◽  
Mohammad Aghazadeh ◽  
Mehdi Yousefi
Keyword(s):  
Escherichia Coli ◽  
Salmonella Spp ◽  
Extended Spectrum ◽  
Shigella Spp ◽  
Klebsiella Spp
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